Rice bran is nutrient-dense milling by-product,but the high content of insoluble dietary fiber(IDF)restricts processing suitability and application in foods.To address this,cellulolytic fungi were isolated from tradit...Rice bran is nutrient-dense milling by-product,but the high content of insoluble dietary fiber(IDF)restricts processing suitability and application in foods.To address this,cellulolytic fungi were isolated from traditional fermented foods.A total of seven fungal strains with clear hydrolysis zones were obtained and identified based on morphology and internal transcribed spacer sequencing.Their growth behavior,carbon and nitrogen utilization,and enzyme activities were further assessed to compare their metabolic capacities.Solid-state fermentation using each isolate induced distinct changes in dietary fiber composition of rice bran.Among the isolates,HTDQt-JL2 showed the most pronounced effect during SSF,yielding the highest conversion of IDF to soluble dietary fiber along with substantial reductions in cellulose and hemicellulose content.Genomic analysis of HTDQt-JL2 revealed an extensive set of genes encoding carbohydrate-active enzymes,particularly those involved in plant cell wall degradation,consistent with its observed fermentation efficiency.These findings indicate that HTDQt-JL2 is an effective microbial candidate for rice bran modification,enhancing the physicochemical properties and expanding the potential as a functional food ingredient.展开更多
基金funded by National Natural Science Foundation of China(32330081,32302136)support R&D Program of Beijing Municipal Education Commission(KM202310011011).
文摘Rice bran is nutrient-dense milling by-product,but the high content of insoluble dietary fiber(IDF)restricts processing suitability and application in foods.To address this,cellulolytic fungi were isolated from traditional fermented foods.A total of seven fungal strains with clear hydrolysis zones were obtained and identified based on morphology and internal transcribed spacer sequencing.Their growth behavior,carbon and nitrogen utilization,and enzyme activities were further assessed to compare their metabolic capacities.Solid-state fermentation using each isolate induced distinct changes in dietary fiber composition of rice bran.Among the isolates,HTDQt-JL2 showed the most pronounced effect during SSF,yielding the highest conversion of IDF to soluble dietary fiber along with substantial reductions in cellulose and hemicellulose content.Genomic analysis of HTDQt-JL2 revealed an extensive set of genes encoding carbohydrate-active enzymes,particularly those involved in plant cell wall degradation,consistent with its observed fermentation efficiency.These findings indicate that HTDQt-JL2 is an effective microbial candidate for rice bran modification,enhancing the physicochemical properties and expanding the potential as a functional food ingredient.
