Diplodia tip blight,caused by Diplodia sapinea,is a global pine necrotic disease causing heavy economic losses to the pine industry.Chemical control,its main current management,easily induces pathogen resistance and e...Diplodia tip blight,caused by Diplodia sapinea,is a global pine necrotic disease causing heavy economic losses to the pine industry.Chemical control,its main current management,easily induces pathogen resistance and environmental pollution,which biological control avoids.This study investigated juniper essential oil’s efficacy against the disease on Mongolian Scots pine(Pinus sylvestris var.mongolica)and its induced resistance mechanisms via pot experiments,physiological assays(defense enzyme activities,resistant substances)and metabolomic sequencing(secondary metabolites).Results showed varied efficacy:three foliar sprays of 10μL mL^(-1) oil achieved the best control phenylalanine ammonia-lyase(PAL),polyphenol oxidase(82.9%).The 20μL mL^(-1) treatment significantly increased(PPO)activities,and contents of lignin,flavonoids and total phenolics.Metabolomic analysis showed 326 upregulated and 527 downregulated different metabolites in essential oilinduced and pathogen-inoculated pines,compared to 483 upregulated and 277 downregulated metabolites in noninduced but inoculated pines.The differentially expressed metabolites in treated pines were primarily enriched in pathways related to amino acid metabolism and plant secondary metabolite biosynthesis,with notably increased expression levels of ferulic acid,scopoletin,pipecolic acid,D-proline,and DL-arginine.Therefore,juniper essential oil protects against D.sapinea by inducing systemic acquired resistance in Mongolian Scots pine.In conclusion,juniper essential oil controls D.sapinea by inducing systemic acquired resistance(SAR)in Mongolian Scots pine,clarifying the molecular mechanism and supporting biological control of the disease.展开更多
Ferritin, a universal intracellular protein, can store large amounts of iron and improve plant resistance to abiotic and biotic stress. In this study, a ferritin gene(TaFer) from Tamarix androssowii Litv. was transfer...Ferritin, a universal intracellular protein, can store large amounts of iron and improve plant resistance to abiotic and biotic stress. In this study, a ferritin gene(TaFer) from Tamarix androssowii Litv. was transferred into Populus tomentosa Carr. cv 'BJR01' via Agrobacterium. Six independent transgenic lines were obtained with a tolerance to kanamycin and three were randomly selected for further analysis. The PCR and RT-PCR results indicate that the TaFer gene had been integrated into the poplar genome. The effect of the gene on abiotic stress tolerance was tested, and the results show that transgenic plants improve growth, had higher chlorophyll and lower MDA contents, and higher relative electrical conductivity,fewer changes of SOD and POD activities, higher iron content, higher root ferric reductase activity and lower levels of ROS accumulation and cell death in response to drought, Fe-insufficient or Fe-excess tolerance. These results indicate that the TaFer gene can improve abiotic stress tolerance in transgenic Populus tomentosa.展开更多
The AP2/ERF family is one of the largest plant-specific transcription factors and plays a vital role in plant growth and stress response.In this study,PsnERF138 was cloned from Populus alba×Populus glandulosa and...The AP2/ERF family is one of the largest plant-specific transcription factors and plays a vital role in plant growth and stress response.In this study,PsnERF138 was cloned from Populus alba×Populus glandulosa and transformed into tobacco using the Agrobacterium-mediated transformation method.PsnERF138 was localized in the nucleus through subcellular localization assay in tobacco.Under normal conditions,the root lengths of PsnERF138 transgenic lines were much longer than those of wild type.Under salt stress,the transgenic tobacco lines over-expressing PsnERF138 showed a significant increase in seed germination rate,plant height,and root length,compared to control plants.In addition,the transgenic tobacco lines displayed some advantages at the physiological level,such as higher superoxide dismutase(SOD)activity,peroxidase(POD)activity,proline content,and lower malondialdehyde(MDA)content,as compared to those in the control plants.Histochemical staining also showed that the transgenic tobacco lines had lower reactive oxygen species(ROS)accumulation,compared to control plants under salt stress.The combined results indicate that poplar PsnERF138 plays a contributing role in augmenting salt tolerance and conferring multiple growth advantages as being overexpressed in tobacco.展开更多
基金supported by the National Key R&D Program of China(2022YFD1401005).
文摘Diplodia tip blight,caused by Diplodia sapinea,is a global pine necrotic disease causing heavy economic losses to the pine industry.Chemical control,its main current management,easily induces pathogen resistance and environmental pollution,which biological control avoids.This study investigated juniper essential oil’s efficacy against the disease on Mongolian Scots pine(Pinus sylvestris var.mongolica)and its induced resistance mechanisms via pot experiments,physiological assays(defense enzyme activities,resistant substances)and metabolomic sequencing(secondary metabolites).Results showed varied efficacy:three foliar sprays of 10μL mL^(-1) oil achieved the best control phenylalanine ammonia-lyase(PAL),polyphenol oxidase(82.9%).The 20μL mL^(-1) treatment significantly increased(PPO)activities,and contents of lignin,flavonoids and total phenolics.Metabolomic analysis showed 326 upregulated and 527 downregulated different metabolites in essential oilinduced and pathogen-inoculated pines,compared to 483 upregulated and 277 downregulated metabolites in noninduced but inoculated pines.The differentially expressed metabolites in treated pines were primarily enriched in pathways related to amino acid metabolism and plant secondary metabolite biosynthesis,with notably increased expression levels of ferulic acid,scopoletin,pipecolic acid,D-proline,and DL-arginine.Therefore,juniper essential oil protects against D.sapinea by inducing systemic acquired resistance in Mongolian Scots pine.In conclusion,juniper essential oil controls D.sapinea by inducing systemic acquired resistance(SAR)in Mongolian Scots pine,clarifying the molecular mechanism and supporting biological control of the disease.
基金supported by Hi-Tech Research and Development Program of China(2013AA102701)Excellent Creative Talents Supporting Program of Heilongjiang University of Chinese Medicine(2012RCQ24)
文摘Ferritin, a universal intracellular protein, can store large amounts of iron and improve plant resistance to abiotic and biotic stress. In this study, a ferritin gene(TaFer) from Tamarix androssowii Litv. was transferred into Populus tomentosa Carr. cv 'BJR01' via Agrobacterium. Six independent transgenic lines were obtained with a tolerance to kanamycin and three were randomly selected for further analysis. The PCR and RT-PCR results indicate that the TaFer gene had been integrated into the poplar genome. The effect of the gene on abiotic stress tolerance was tested, and the results show that transgenic plants improve growth, had higher chlorophyll and lower MDA contents, and higher relative electrical conductivity,fewer changes of SOD and POD activities, higher iron content, higher root ferric reductase activity and lower levels of ROS accumulation and cell death in response to drought, Fe-insufficient or Fe-excess tolerance. These results indicate that the TaFer gene can improve abiotic stress tolerance in transgenic Populus tomentosa.
基金supported by State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University)(201205)the 111 Project(B16010).
文摘The AP2/ERF family is one of the largest plant-specific transcription factors and plays a vital role in plant growth and stress response.In this study,PsnERF138 was cloned from Populus alba×Populus glandulosa and transformed into tobacco using the Agrobacterium-mediated transformation method.PsnERF138 was localized in the nucleus through subcellular localization assay in tobacco.Under normal conditions,the root lengths of PsnERF138 transgenic lines were much longer than those of wild type.Under salt stress,the transgenic tobacco lines over-expressing PsnERF138 showed a significant increase in seed germination rate,plant height,and root length,compared to control plants.In addition,the transgenic tobacco lines displayed some advantages at the physiological level,such as higher superoxide dismutase(SOD)activity,peroxidase(POD)activity,proline content,and lower malondialdehyde(MDA)content,as compared to those in the control plants.Histochemical staining also showed that the transgenic tobacco lines had lower reactive oxygen species(ROS)accumulation,compared to control plants under salt stress.The combined results indicate that poplar PsnERF138 plays a contributing role in augmenting salt tolerance and conferring multiple growth advantages as being overexpressed in tobacco.
