Biomedical implants are susceptible to bacterial colonization,which can lead to challenging implant-associated infections.In particular,dental implant abutments-which are continuously exposed to bacteria within the or...Biomedical implants are susceptible to bacterial colonization,which can lead to challenging implant-associated infections.In particular,dental implant abutments-which are continuously exposed to bacteria within the oral cavity-stand to greatly benefit from strategies which inhibit the development of bacterial biofilms.Liquid-infused titanium surfaces have demonstrated excellent biofilm repellency,but to date have not been analyzed with substances suitable for medical device approval in terms of biocompatibility under conditions mimicking the environment of dental implant abutments.In this study,different medical-grade lubricants coated onto laser-structured titanium were screened for stability and water-repellency-with the results suggesting that unmodified structured titanium coated with silicone oil was the most promising combination of materials.When analyzing biofilm formation,the coated surfaces showed a statistically significant reduction in oral commensal Streptococcus oralis biofilms grown under static conditions as well as oral multispecies biofilms grown under salivation-resembling flow conditions.This biofilm-reducing effect was also observed when the coated surface interfaced with a 3D implant-tissue-oral-bacterial-biofilm(INTERbACT)in vitro model,which allows for the direct interaction of human tissue and oral multispecies biofilm at the implant interface.Importantly,this biofilm reduction was not due to toxicity of the coated surfaces,but is most likely attributable to inhibition of bacterial attachment.Additionally,the surfaces were not cytotoxic,without altering adjacent soft tissue or causing elevated pro-inflammatory cytokine secretion.These findings highlight the promise of biocompatible liquid-infused titanium surfaces as biofilmrepellent implant abutment modifications and provide the basis for further investigations in targeted pre-clinical studies.展开更多
One dimensional(1D)nanostructures attract considerable attention,enabling a broad application owing to their unique properties.However,the precise mechanism of 1D morphology attainment remains a matter of debate.In th...One dimensional(1D)nanostructures attract considerable attention,enabling a broad application owing to their unique properties.However,the precise mechanism of 1D morphology attainment remains a matter of debate.In this study,ultrafast picosecond(ps)laser-induced treatment on upconversion nanoparticles(UCNPs)is offered as a tool for 1 D-nanostructures formation.Fragmentation,reshaping through recrystallization process and bioadaptation of initially hydrophobic(β-Na_(1.5)Y_(1.5_)F_(6):Yb^(3+),Tm^(3+)/β-Na_(1.5)Y_(1.5_)F_(6))core/shell nanoparticles by means of one-step laser treatment in water are demonstrated.“True”1D nanostructures through"Medusa^-like structures can be obtained,maintaining anti-Stokes luminescence functionalities.A matter of the one-dimensional UCNPs based on direction of energy migration processes is debated.The proposed laser treatment approach is suitable for fast UCNP surface modification and nano-to-nano transformation,that open unique opportunities to expand UCNP applications in industry and biomedicine.展开更多
基金funded by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)under the Collaborative Research Center SFB/TRR-298-SIIRI-Project ID 426335750the Deutsche Gesellschaft für Prothetische Zahnmedizin und Biomaterialien e.V.(DGPro,German Society for Prosthetic Dentistry and Biomaterials).
文摘Biomedical implants are susceptible to bacterial colonization,which can lead to challenging implant-associated infections.In particular,dental implant abutments-which are continuously exposed to bacteria within the oral cavity-stand to greatly benefit from strategies which inhibit the development of bacterial biofilms.Liquid-infused titanium surfaces have demonstrated excellent biofilm repellency,but to date have not been analyzed with substances suitable for medical device approval in terms of biocompatibility under conditions mimicking the environment of dental implant abutments.In this study,different medical-grade lubricants coated onto laser-structured titanium were screened for stability and water-repellency-with the results suggesting that unmodified structured titanium coated with silicone oil was the most promising combination of materials.When analyzing biofilm formation,the coated surfaces showed a statistically significant reduction in oral commensal Streptococcus oralis biofilms grown under static conditions as well as oral multispecies biofilms grown under salivation-resembling flow conditions.This biofilm-reducing effect was also observed when the coated surface interfaced with a 3D implant-tissue-oral-bacterial-biofilm(INTERbACT)in vitro model,which allows for the direct interaction of human tissue and oral multispecies biofilm at the implant interface.Importantly,this biofilm reduction was not due to toxicity of the coated surfaces,but is most likely attributable to inhibition of bacterial attachment.Additionally,the surfaces were not cytotoxic,without altering adjacent soft tissue or causing elevated pro-inflammatory cytokine secretion.These findings highlight the promise of biocompatible liquid-infused titanium surfaces as biofilmrepellent implant abutment modifications and provide the basis for further investigations in targeted pre-clinical studies.
基金the Ministry of Science and Higher Education within the State assignment FSRC《Crystallography and Photonics》RAS in part of《UCNP synthesis》,by the Russian Foundation for Basic Research according to the research projects No 18-29-20064 in the part of《PL analysis》and Ns 20-32-70174 in the part o f《complex structures analysis》,by the Russian Science Foundation project No18-79-10198 in the part of《UCNP analysis》.BC acknowledges financial support from Lower Saxony through“Quanten und Nanometrologie”project(QUANOMET)and DFG Cluster of Excellence PhoenixD(EXC 2122,Project ID 390833453).
文摘One dimensional(1D)nanostructures attract considerable attention,enabling a broad application owing to their unique properties.However,the precise mechanism of 1D morphology attainment remains a matter of debate.In this study,ultrafast picosecond(ps)laser-induced treatment on upconversion nanoparticles(UCNPs)is offered as a tool for 1 D-nanostructures formation.Fragmentation,reshaping through recrystallization process and bioadaptation of initially hydrophobic(β-Na_(1.5)Y_(1.5_)F_(6):Yb^(3+),Tm^(3+)/β-Na_(1.5)Y_(1.5_)F_(6))core/shell nanoparticles by means of one-step laser treatment in water are demonstrated.“True”1D nanostructures through"Medusa^-like structures can be obtained,maintaining anti-Stokes luminescence functionalities.A matter of the one-dimensional UCNPs based on direction of energy migration processes is debated.The proposed laser treatment approach is suitable for fast UCNP surface modification and nano-to-nano transformation,that open unique opportunities to expand UCNP applications in industry and biomedicine.
