Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may...Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.展开更多
The Quadric Error Metrics(QEM)algorithm is a widely used method for mesh simplification;however,it often struggles to preserve high-frequency geometric details,leading to the loss of salient features.To address this l...The Quadric Error Metrics(QEM)algorithm is a widely used method for mesh simplification;however,it often struggles to preserve high-frequency geometric details,leading to the loss of salient features.To address this limitation,we propose the Salient Feature Sampling Points-based QEM(SFSP-QEM)—also referred to as the Deep Learning-Based Salient Feature-Preserving Algorithm for Mesh Simplification—which incorporates a Salient Feature-Preserving Point Sampler(SFSP).This module leverages deep learning techniques to prioritize the preservation of key geometric features during simplification.Experimental results demonstrate that SFSP-QEM significantly outperforms traditional QEM in preserving geometric details.Specifically,for general models from the Stanford 3D Scanning Repository,which represent typical mesh structures used in mesh simplification benchmarks,the Hausdorff distance of simplified models using SFSP-QEM is reduced by an average of 46.58% compared to those simplified using traditional QEM.In customized models such as the Zigong Lantern used in cultural heritage preservation,SFSP-QEM achieves an average reduction of 28.99% in Hausdorff distance.Moreover,the running time of this method is only 6%longer than that of traditional QEM while significantly improving the preservation of geometric details.These results demonstrate that SFSP-QEMis particularly effective for applications requiring high-fidelity simplification while retaining critical features.展开更多
Hydroformylation of olefins is one of the highest-volume industrial reactions to meet the vast demands for aldehydes as well as their derivatives.Homogeneous Co complexes were the original catalysts industrialized sin...Hydroformylation of olefins is one of the highest-volume industrial reactions to meet the vast demands for aldehydes as well as their derivatives.Homogeneous Co complexes were the original catalysts industrialized since 1960s.Heterogeneous catalysis is considered superior owing to the facile separation of catalysts from products,shorter technical process,and reduced manufacturing costs.Unexpectedly,there has not been a single case of plant using heterogenized Co-based catalyst successfully.To address the separation issue and understand the catalytic mechanism of the reactions,this review summarizes the progress in heterogeneous systems and provides a detailed discussion of their catalytic performance.Strategies for stabilizing Co species through support modification and additive incorporation are carefully considered to elucidate why heterogeneous systems have not yet succeeded on an industrial scale.Furthermore,we provide our insights for the development of heterogeneous catalytic hydroformylation,including the challenges,opportunities,and outlooks.The aim is to deepen the fundamental understanding of heterogeneous alkene hydroformylation,guiding the community's research efforts towards realizing its successful application in the future.展开更多
Over more than a decade of development,medium to deep shale gas reservoirs have faced rapid production declines,making sustained output challenging.To harness remaining reserves effectively,advanced fracturing techniq...Over more than a decade of development,medium to deep shale gas reservoirs have faced rapid production declines,making sustained output challenging.To harness remaining reserves effectively,advanced fracturing techniques such as infill drilling are essential.This study develops a complex fracture network model for dual horizontal wells and a four-dimensional in-situ stress evolution model,grounded in elastic porous media theory.These models simulate and analyze the evolution of formation pore pressure and in-situ stress during production.The investigation focuses on the influence of infill well fracturing timing on fracture propagation patterns,individual well productivity,and the overall productivity of well clusters.The findings reveal that,at infill well locations,the maximum horizontal principal stress undergoes the most significant reduction,while changes in the minimum horizontal principal stress and vertical stress remain minimal.The horizontal stress surrounding the infill well may reorient,potentially transitioning the stress regime from strike-slip to normal faulting.Delays in infill well fracturing increase lateral fracture deflection and diminish fracture propagation between wells.Considering the stable production phase and cumulative gas output of the well group,the study identifies an optimal timing for infill fracturing.Notably,larger well spacing shifts the optimal timing to a later stage.展开更多
With the advent of the digital economy,there has been a rapid proliferation of small-scale Internet data centers(SIDCs).By leveraging their spatiotemporal load regulation potential through data workload balancing,aggr...With the advent of the digital economy,there has been a rapid proliferation of small-scale Internet data centers(SIDCs).By leveraging their spatiotemporal load regulation potential through data workload balancing,aggregated SIDCs have emerged as promising demand response(DR)resources for future power distribution systems.This paper presents an innovative framework for assessing capacity value(CV)by aggregating SIDCs participating in DR programs(SIDC-DR).Initially,we delineate the concept of CV tailored for aggregated SIDC scenarios and establish a metric for the assessment.Considering the effects of the data load dynamics,equipment constraints,and user behavior,we developed a sophisticated DR model for aggregated SIDCs using a data network aggregation method.Unlike existing studies,the proposed model captures the uncertainties associated with end tenant decisions to opt into an SIDC-DR program by utilizing a novel uncertainty modeling approach called Z-number formulation.This approach accounts for both the uncertainty in user participation intentions and the reliability of basic information during the DR process,enabling high-resolution profiling of the SIDC-DR potential in the CV evaluation.Simulation results from numerical studies conducted on a modified IEEE-33 node distribution system confirmed the effectiveness of the proposed approach and highlighted the potential benefits of SIDC-DR utilization in the efficient operation of future power systems.展开更多
AIM:To compare the visual outcomes and corneal higherorder aberrations(HOAs)of patients with high or low myopic astigmatism after small incision lenticule extraction(SMILE).METHODS:A total of 157 eyes of 157 patients ...AIM:To compare the visual outcomes and corneal higherorder aberrations(HOAs)of patients with high or low myopic astigmatism after small incision lenticule extraction(SMILE).METHODS:A total of 157 eyes of 157 patients who underwent SMILE were included in this retrospective,nonrandomized,comparative study.All the eyes which were with the rule astigmatism were divided into high astigmatism group(HAG;astigmatism≤-2.00 D,73 eyes)and low astigmatism group(LAG;astigmatism≥-1.00 D,84 eyes).Visual and refractive examinations were performed,HOAs of the anterior surface,posterior surface,and total cornea of the eyes were evaluated preoperatively and 6mo postoperatively.RESULTS:At the postoperative 6-month follow-up,uncorrected distance visual acuity of 20/20 or better was achieved in 97%and 100%eyes in HAG and LAG respectively and 74%and 100%eyes were within-0.50 D.Vector analysis revealed no significant differences in the correction index(P=0.066),angle of error(P=0.091)or flattening index(P=0.987)between two groups.The magnitude of error was-0.37±0.31 D in HAG and-0.04±0.19 D in LAG(P<0.001).Index of success(IOS)was 0.22±0.09 in the HAG and 0.50±0.46 in the LAG(P<0.001).HOAs of most anterior,posterior and total cornea significantly increased after SMILE,especially the spherical aberration and coma.For HAG,the SMILE procedure induced significantly higher anterior,posterior and total cornea horizontal coma and total corneal total HOAs compared with LAG(P<0.001)and these surgically induced HOAs predominantly originated from the anterior surface of the cornea.CONCLUSION:SMILE surgery induces more HOAs and a mild under-correction of astigmatism in eyes with high astigmatism.The increment in HOAs after SMILE is related to preoperative astigmatism.展开更多
AIM:To investigate the changes in posterior corneal elevation within 6mo after small incision lenticule extraction(SMILE)surgery for myopia and myopic astigmatism in patients with thin corneas.METHODS:A prospective st...AIM:To investigate the changes in posterior corneal elevation within 6mo after small incision lenticule extraction(SMILE)surgery for myopia and myopic astigmatism in patients with thin corneas.METHODS:A prospective study included patients with thin corneas(preoperative thinnest corneal thickness ranging from 480 to 520μm)who underwent SMILE for myopia or myopic astigmatism.Corneal topography and posterior corneal elevation were assessed using Pentacam HR at three time points:preoperatively,1mo,and 6mo postoperatively.The measured parameters included thinnest point elevation(PTE),posterior maximal elevation(PME),posterior central elevation(PCE),and 24 additional reference points.RESULTS:A total of 106 eyes from 106 patients(age range:18-34)were included in the study.Uncorrected distance visual acuity(UDVA)improved significantly,with a mean logMAR value of-0.07±0.06 at the final follow-up visit.Measurements of posterior corneal elevation showed no significant changes in most points,hemispheres,and meridians at 6mo postoperatively.Notably,only two points,ΔE_(2mm-45°)andΔE_(2mm-90°),exhibited statistically significant elevation changes:the elevation ofΔE_(2mm-45°)increased from-2.3±4.99 to-1.0±5.9μm(P=0.0037),and that ofΔE_(2mm-90°)increased from-16±7.53 to-15±7.4μm(P=0.016).However,these changes were within the measurement error range of the Pentacam HR(±5μm in a 5 mm area).CONCLUSION:SMILE surgery is a safe and stable procedure for correcting myopia or myopic astigmatism in patients with thin corneas,as evidenced by the stability of posterior corneal elevation.展开更多
Sensor nodes cannot directly communicate with the distant unmanned aerial vehicle( UAV) for their low transmission power. Distributed collaborative beamforming from sensor nodes within a cluster is proposed to provide...Sensor nodes cannot directly communicate with the distant unmanned aerial vehicle( UAV) for their low transmission power. Distributed collaborative beamforming from sensor nodes within a cluster is proposed to provide high speed data transmission to the distant UAV. The bit error ratio( BER) closed-form expression of distributed collaborative beamforming transmission with mobile sensor nodes has been derived. Furthermore,based on the theoretical BER analysis and the numerical results,we have analyzed the impacts of nodes 'mobility,number of sensor nodes,transmission power and the elevation angle of UAV on the BER performance of collaborative beamforming. And we come to the following conclusions: the mobility of sensor nodes largely decreases the BER performance; when the position deviation radius is large,incensement in power cannot improve BER anymore; the size of cluster should be bigger than 10 for the purpose of achieving good BER performance in Rayleigh fading channel.展开更多
The hypothalamic-pituitary-ovarian(HPO)axis represents a central neuroendocrine network essential for reproductive function.Despite its critical role,the intrinsic heterogeneity within the HPO axis across vertebrates ...The hypothalamic-pituitary-ovarian(HPO)axis represents a central neuroendocrine network essential for reproductive function.Despite its critical role,the intrinsic heterogeneity within the HPO axis across vertebrates and the complex intercellular interactions remain poorly defined.This study provides the first comprehensive,unbiased,cell type-specific molecular profiling of all three components of the HPO axis in adult Lohmann layers and Liangshan Yanying chickens.Within the hypothalamus,pituitary,and ovary,seven,12,and 13 distinct cell types were identified,respectively.Results indicated that the pituitary adenylate cyclase activating polypeptide(PACAP),follicle-stimulating hormone(FSH),and prolactin(PRL)signaling pathways may modulate the synthesis and secretion of gonadotropin-releasing hormone(GnRH),FSH,and luteinizing hormone(LH)within the hypothalamus and pituitary.In the ovary,interactions between granulosa cells and oocytes involved the KIT,CD99,LIFR,FN1,and ANGPTL signaling pathways,which collectively regulate follicular maturation.The SEMA4 signaling pathway emerged as a critical mediator across all three tissues of the HPO axis.Additionally,gene expression analysis revealed that relaxin 3(RLN3),gastrin-releasing peptide(GRP),and cocaine-and amphetamine regulated transcripts(CART,also known as CARTPT)may function as novel endocrine hormones,influencing the HPO axis through autocrine,paracrine,and endocrine pathways.Comparative analyses between Lohmann layers and Liangshan Yanying chickens demonstrated higher expression levels of GRP,RLN3,CARTPT,LHCGR,FSHR,and GRPR in the ovaries of Lohmann layers,potentially contributing to their superior reproductive performance.In conclusion,this study provides a detailed molecular characterization of the HPO axis,offering novel insights into the regulatory mechanisms underlying reproductive biology.展开更多
Esophageal disease is a common disorder of the digestive system that can severely affect the quality of life andprognosis of patients. Esophageal stenting is an effective treatment that has been widely used in clinica...Esophageal disease is a common disorder of the digestive system that can severely affect the quality of life andprognosis of patients. Esophageal stenting is an effective treatment that has been widely used in clinical practice.However, esophageal stents of different types and parameters have varying adaptability and effectiveness forpatients, and they need to be individually selected according to the patient’s specific situation. The purposeof this study was to provide a reference for clinical doctors to choose suitable esophageal stents. We used 3Dprinting technology to fabricate esophageal stents with different ratios of thermoplastic polyurethane (TPU)/(Poly-ε-caprolactone) PCL polymer, and established an artificial neural network model that could predict the radial forceof esophageal stents based on the content of TPU, PCL and print parameter. We selected three optimal ratios formechanical performance tests and evaluated the biomechanical effects of different ratios of stents on esophagealimplantation, swallowing, and stent migration processes through finite element numerical simulation and in vitrosimulation tests. The results showed that different ratios of polymer stents had different mechanical properties,affecting the effectiveness of stent expansion treatment and the possibility of postoperative complications of stentimplantation.展开更多
Existing studies contend that latent heating(LH)will replace sensible heating(SH)to become the dominant factor affecting the development of the Tibetan Plateau vortex(TPV)after it moves off the Tibetan Plateau(TP).How...Existing studies contend that latent heating(LH)will replace sensible heating(SH)to become the dominant factor affecting the development of the Tibetan Plateau vortex(TPV)after it moves off the Tibetan Plateau(TP).However,in the process of the TPV moving off the TP requires that the airmass traverse the eastern slope of the Tibetan Plateau(ESTP)where the topography and diabatic heating(DH)conditions rapidly change.How LH gradually replaces SH to become the dominant factor in the development of the TPV over the ESTP is still not very clear.In this paper,an analysis of a typical case of a TPV with a long life history over the ESTP is performed by using multi-sourced meteorological data and model simulations.The results show that SH from the TP surface can change the TPV-associated precipitation distribution by temperature advection after the TPV moves off the TP.The LH can then directly promote the development of the TPV and has a certain guiding effect on the track of the TPV.The SH can control the active area of LH by changing the falling area of the TPV-associated precipitation,so it still plays a key role in the development and tracking of the TPV even though it has moved out of the main body of the TP.展开更多
Casing deformation and frac-hit pose significant challenges to the development of deep shale gas in southern Sichuan Basin.By analyzing the mechanism and main control factors of casing deformation and frac-hit,two kin...Casing deformation and frac-hit pose significant challenges to the development of deep shale gas in southern Sichuan Basin.By analyzing the mechanism and main control factors of casing deformation and frac-hit,two kinds of risk assessment methods were defined,and the overall prevention and control concept and practice were formulated.The results show that initial stress,pore pressure,fault development and large scale fracturing in local block are the main factors leading to the deformation.The development of fracture through well group and uncontrolled fracturing fluid volume are the main factors leading to pressure channeling.Based on this,the risk classification technology of casing deformation and frac-hit is established,and the dual-optimal,dual-control concept and technology are formed.In terms of the prevention and control of casing deformation,the formation of small-diameter bridge plug fracturing,large section combined fracturing,glass beads cementing,singlewell staggered and platform straddle fracturing mode,dual-dimension controlled and lift fracturing,hyperbolic diagnosis,etc.Frac-hit prevention and control formed pump sequence optimization mode,physical and chemical temporary plugging and other methods.The above technology achieved casing deformation rate decreased from 50.4%to 25.4%,frac-hit rate decreased from 58.6%to 33.9%,and the average well kilometer EUR reached 0.52e0.7 million square meters,an increase of 7.7%compared with the previous research,with remarkable results.展开更多
Proper cellular metabolism in T cells is critical for a productive immune response.However,when dysregulated by intrinsic or extrinsic metabolic factors,T cells may contribute to a wide spectrum of diseases,such as ca...Proper cellular metabolism in T cells is critical for a productive immune response.However,when dysregulated by intrinsic or extrinsic metabolic factors,T cells may contribute to a wide spectrum of diseases,such as cancers and autoimmune diseases.However,the metabolic regulation of T cells remains incompletely understood.Here,we show that MYO1F is required for human and mouse T-cell activation after TCR stimulation and that T-cell-specific Myo1f knockout mice exhibit an increased tumor burden and attenuated EAE severity due to impaired T-cell activation in vivo.Mechanistically,after TCR stimulation,MYO1F is phosphorylated by LCK at tyrosines 607 and 634,which is critical for glyceraldehyde-3-phosphate dehydrogenase(GAPDH)acetylation at Lys84,86 and 227 mediated byα-TAT1,which is an acetyltransferase,and these processes are important for its activation,cellular glycolysis and thus the effector function of T cells.Importantly,we show that a fusion protein of VAV1-MYO1F,a recurrent peripheral T-cell lymphoma(PTCL)-associated oncogenic protein,promotes hyperacetylation of GAPDH and its activation,which leads to aberrant glycolysis and T-cell proliferation,and that inhibition of the activity of GAPDH significantly limits T-cell activation and proliferation and extends the survival of hVAV1-MYO1F knock-in mice.Moreover,hyperacetylation of GAPDH was confirmed in human PTCL patient samples containing the VAV1-MYO1F gene fusion.Overall,this study revealed not only the mechanisms by which MYO1F regulates T-cell metabolism and VAV1-MYO1F fusion-induced PTCL but also promising therapeutic targets for the treatment of PTCL.展开更多
In grey system theory,the studies in the field of grey prediction model are focused on real number sequences,rather than grey number ones.Hereby,a prediction model based on interval grey number sequences is proposed.B...In grey system theory,the studies in the field of grey prediction model are focused on real number sequences,rather than grey number ones.Hereby,a prediction model based on interval grey number sequences is proposed.By mining the geometric features of interval grey number sequences on a two-dimensional surface,all the interval grey numbers are converted into real numbers by means of certain algorithm,and then the prediction model is established based on those real number sequences.The entire process avoids the algebraic operations of grey number,and the prediction problem of interval grey number is usefully solved.Ultimately,through an example＇s program simulation,the validity and practicability of this novel model are verified.展开更多
Objective:To explore the protective effect and its molecular mechanism of apoptosis signalregulating kinase 1(ASK1) inhibitor(GS-459679) on acetaminophen-induced liver injury in mice.Methods:The model of liver injury ...Objective:To explore the protective effect and its molecular mechanism of apoptosis signalregulating kinase 1(ASK1) inhibitor(GS-459679) on acetaminophen-induced liver injury in mice.Methods:The model of liver injury was established by administration of acetaminophen(APAP)(300 mg/kg,i.p.) on C57BL/6 mice.Forty-eight male C57BL/6 mice were randomly divided into four groups,consisting of control group,GS group(GS-459679,30 mg/kg,i.p.),APAPinduced group,and GS combined with APAP-induced group.For GS combined with APAPinduced group,mice were treated with GS 30 min prior to administration of APAP.After mice were euthanized at 6 h or 12 h.respectively,serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were analyzed,and mRNA levels of TNF- α,IL-6 and IL-1βwere tested.The activity of glutathione(GSH),oxidized GSH(GSSG) and malondialdehyde were quantified.In addition,ASK1,P-ASK1,JNK and P-JNK protein levels were tested in all groups.Results:The ASK1 and P-ASK1 levels were up-regulated in APAP-induced group.Compared to the control group,serum levels of ALT and AST.and mRNA levels of TNF- a,IL-6 and IL-1(3were increased in APAP-induced group.Meanwhile,the levels of MAD and GSSG.and the ratio of GSSG/GSH were higher and the JNK was activatedin APAP-induced group compared with that in control group.However,compared to APAP-induced group,GS combined with APAP-induced group displayed a decrease of protein expression levels of ASK 1,P-ASKI and P-JNK,a reduction of serum levels of ALT and AST,a decrease in TNF- a.IL-6 and IL-1(3 mRNA levels,and a low ration of GSSG/GSH.Conclusions:GS-459679 treatment effectively down-regulates ASK1 and P-ASK 1 expression.Addition of GS-459679 decreases the generation of liver metabolites and inflammatory factors,reduces oxidative stress reaction,inhibits JNK activation,and then protects the responsiveness to APAP-induced liver injury.展开更多
Infection with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) causes diverse clinical manifestations and tissue injuries in multiple organs.However, cellular and molecular understanding of SARS-CoV-2 infe...Infection with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) causes diverse clinical manifestations and tissue injuries in multiple organs.However, cellular and molecular understanding of SARS-CoV-2 infection-associated pathology and immune defense features in different organs remains incomplete. Here, we profiled approximately 77 000single-nucleus transcriptomes of the lung, liver,kidney, and cerebral cortex in rhesus macaques(Macaca mulatta) infected with SARS-CoV-2 and healthy controls. Integrated analysis of the multiorgan dataset suggested that the liver harbored the strongest global transcriptional alterations. We observed prominent impairment in lung epithelial cells, especially in AT2 and ciliated cells, and evident signs of fibrosis in fibroblasts. These lung injury characteristics are similar to those reported in patients with coronavirus disease 2019(COVID-19).Furthermore, we found suppressed MHC class I/II molecular activity in the lung, inflammatory response in the liver, and activation of the kynurenine pathway,which induced the development of an immunosuppressive microenvironment. Analysis of the kidney dataset highlighted tropism of tubule cells to SARS-CoV-2, and we found membranous nephropathy(an autoimmune disease) caused by podocyte dysregulation. In addition, we identified the pathological states of astrocytes and oligodendrocytes in the cerebral cortex, providing molecular insights into COVID-19-related neurological implications. Overall, our multi-organ single-nucleus transcriptomic survey of SARS-CoV-2-infected rhesus macaques broadens our understanding of disease features and antiviral immune defects caused by SARS-CoV-2 infection,which may facilitate the development of therapeutic interventions for COVID-19.展开更多
AIM:To investigate the effect of amniotic membrane covering(AMC) on the healing of cornea epithelium and visual acuity for fungal keratitis after debridement.METHODS:Twenty fungal keratitis patients were divided into ...AIM:To investigate the effect of amniotic membrane covering(AMC) on the healing of cornea epithelium and visual acuity for fungal keratitis after debridement.METHODS:Twenty fungal keratitis patients were divided into two groups randomly, the AMC group and the control group, ten patients each group. Both debridement of the infected cornea tissue and standard anti-fungus drugs treatments were given to every patients, monolayer amniotic membrane were sutured to the surface of the entire cornea and bulbar conjunctiva with 10-0 nylon suture for patients in the AMC group.The diameter of the ulcer was determined with slit lamp microscope and the depth of the infiltration was determined with anterior segment optical coherence tomography. Uncorrected visual acuity(UCVA) was tested before surgery and three month after healing of the epithelial layer. The healing time of the cornea epithelium, visual acuity(VA) was compared between the two groups using t- test.RESULTS:There was no statistical difference of the diameter of the ulcer, depth of the infiltration, height of the hypopyon and VA between the two groups beforesurgery(P 】0.05). The average healing time of the AMC group was 6.89 ±2.98 d, which was statistically shorter than that of the control group(10.23±2.78d)(P 【0.05).The average UCVA of the AMC group was 0.138 ±0.083,which was statistically better than that of the control group(0.053±0.068)(P 【0.05).CONCLUSION:AMC surgery could promote healing of cornea epithelium after debridement for fungal keratitis and lead to better VA outcome.展开更多
Bladder cancer is the most common malignant tumor in the urinary system,with high morbidity,mortality and recurrence after surgery.However,current bladder cancer urine diagnosis methods are limited by the low accuracy...Bladder cancer is the most common malignant tumor in the urinary system,with high morbidity,mortality and recurrence after surgery.However,current bladder cancer urine diagnosis methods are limited by the low accuracy and specificity due to the low abundance of bladder cancer biomarkers in the urine with complex biological environments.Herein,we present a high stability indium gallium zinc oxide field effect transistor(IGZO-FET)biosensor for efficient identification of bladder cancer biomarkers from human urine samples.The recognition molecular functionalized IGZO-FET biosensor exhibits stable electronic and sensing performance due to the large-area fabrication of IGZO thin-film FET.Owing to the excellent electrical performance of IGZO-FET,the IGZO-FET biosensor exhibits high sensitivity and extremely low detection limit(2.7 amol/L)towards bladder cancer biomarkers.The IGZO-FET biosensor is also able to directly detect bladder tumor biomarker in human urine with high sensitivity and specificity,and could differentiate bladder cancer patients’urine samples from healthy donors effectively.These results indicate that our designed high-performance biosensor shows great potential in the application of portable digital bladder cancer diagnosis devices.展开更多
基金supported partly by the National Natural Science Foundation of China,No.82071332the Chongqing Natural Science Foundation Joint Fund for Innovation and Development,No.CSTB2023NSCQ-LZX0041 (both to ZG)。
文摘Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.
基金Our research was funded by the Sichuan Key Provincial Research Base of Intelligent Tourism(No.ZHZJ23-02)supported by the Scientific Research and Innovation Team Program of Sichuan University of Science and Engineering(No.SUSE652A006)+1 种基金Additional support was provided by the National Cultural and Tourism Science and Technology Innovation Research andDevelopment Project(No.202417)the Lantern Culture and Crafts Innovation Key Laboratory Project of the Sichuan ProvincialDepartment of Culture and Tourism(No.SCWLCD-A02).
文摘The Quadric Error Metrics(QEM)algorithm is a widely used method for mesh simplification;however,it often struggles to preserve high-frequency geometric details,leading to the loss of salient features.To address this limitation,we propose the Salient Feature Sampling Points-based QEM(SFSP-QEM)—also referred to as the Deep Learning-Based Salient Feature-Preserving Algorithm for Mesh Simplification—which incorporates a Salient Feature-Preserving Point Sampler(SFSP).This module leverages deep learning techniques to prioritize the preservation of key geometric features during simplification.Experimental results demonstrate that SFSP-QEM significantly outperforms traditional QEM in preserving geometric details.Specifically,for general models from the Stanford 3D Scanning Repository,which represent typical mesh structures used in mesh simplification benchmarks,the Hausdorff distance of simplified models using SFSP-QEM is reduced by an average of 46.58% compared to those simplified using traditional QEM.In customized models such as the Zigong Lantern used in cultural heritage preservation,SFSP-QEM achieves an average reduction of 28.99% in Hausdorff distance.Moreover,the running time of this method is only 6%longer than that of traditional QEM while significantly improving the preservation of geometric details.These results demonstrate that SFSP-QEMis particularly effective for applications requiring high-fidelity simplification while retaining critical features.
文摘Hydroformylation of olefins is one of the highest-volume industrial reactions to meet the vast demands for aldehydes as well as their derivatives.Homogeneous Co complexes were the original catalysts industrialized since 1960s.Heterogeneous catalysis is considered superior owing to the facile separation of catalysts from products,shorter technical process,and reduced manufacturing costs.Unexpectedly,there has not been a single case of plant using heterogenized Co-based catalyst successfully.To address the separation issue and understand the catalytic mechanism of the reactions,this review summarizes the progress in heterogeneous systems and provides a detailed discussion of their catalytic performance.Strategies for stabilizing Co species through support modification and additive incorporation are carefully considered to elucidate why heterogeneous systems have not yet succeeded on an industrial scale.Furthermore,we provide our insights for the development of heterogeneous catalytic hydroformylation,including the challenges,opportunities,and outlooks.The aim is to deepen the fundamental understanding of heterogeneous alkene hydroformylation,guiding the community's research efforts towards realizing its successful application in the future.
基金supported by the National Natural Science Foundation of China(Grant No.52374043)the Southwest Oil&Gas Field Branch in PetroChina(Grant No.JS2023-115)。
文摘Over more than a decade of development,medium to deep shale gas reservoirs have faced rapid production declines,making sustained output challenging.To harness remaining reserves effectively,advanced fracturing techniques such as infill drilling are essential.This study develops a complex fracture network model for dual horizontal wells and a four-dimensional in-situ stress evolution model,grounded in elastic porous media theory.These models simulate and analyze the evolution of formation pore pressure and in-situ stress during production.The investigation focuses on the influence of infill well fracturing timing on fracture propagation patterns,individual well productivity,and the overall productivity of well clusters.The findings reveal that,at infill well locations,the maximum horizontal principal stress undergoes the most significant reduction,while changes in the minimum horizontal principal stress and vertical stress remain minimal.The horizontal stress surrounding the infill well may reorient,potentially transitioning the stress regime from strike-slip to normal faulting.Delays in infill well fracturing increase lateral fracture deflection and diminish fracture propagation between wells.Considering the stable production phase and cumulative gas output of the well group,the study identifies an optimal timing for infill fracturing.Notably,larger well spacing shifts the optimal timing to a later stage.
基金supported in part by the National Natural Science Foundation of China under Grant 52177082in part by the Beijing Nova Program under Grant 20220484007.
文摘With the advent of the digital economy,there has been a rapid proliferation of small-scale Internet data centers(SIDCs).By leveraging their spatiotemporal load regulation potential through data workload balancing,aggregated SIDCs have emerged as promising demand response(DR)resources for future power distribution systems.This paper presents an innovative framework for assessing capacity value(CV)by aggregating SIDCs participating in DR programs(SIDC-DR).Initially,we delineate the concept of CV tailored for aggregated SIDC scenarios and establish a metric for the assessment.Considering the effects of the data load dynamics,equipment constraints,and user behavior,we developed a sophisticated DR model for aggregated SIDCs using a data network aggregation method.Unlike existing studies,the proposed model captures the uncertainties associated with end tenant decisions to opt into an SIDC-DR program by utilizing a novel uncertainty modeling approach called Z-number formulation.This approach accounts for both the uncertainty in user participation intentions and the reliability of basic information during the DR process,enabling high-resolution profiling of the SIDC-DR potential in the CV evaluation.Simulation results from numerical studies conducted on a modified IEEE-33 node distribution system confirmed the effectiveness of the proposed approach and highlighted the potential benefits of SIDC-DR utilization in the efficient operation of future power systems.
文摘AIM:To compare the visual outcomes and corneal higherorder aberrations(HOAs)of patients with high or low myopic astigmatism after small incision lenticule extraction(SMILE).METHODS:A total of 157 eyes of 157 patients who underwent SMILE were included in this retrospective,nonrandomized,comparative study.All the eyes which were with the rule astigmatism were divided into high astigmatism group(HAG;astigmatism≤-2.00 D,73 eyes)and low astigmatism group(LAG;astigmatism≥-1.00 D,84 eyes).Visual and refractive examinations were performed,HOAs of the anterior surface,posterior surface,and total cornea of the eyes were evaluated preoperatively and 6mo postoperatively.RESULTS:At the postoperative 6-month follow-up,uncorrected distance visual acuity of 20/20 or better was achieved in 97%and 100%eyes in HAG and LAG respectively and 74%and 100%eyes were within-0.50 D.Vector analysis revealed no significant differences in the correction index(P=0.066),angle of error(P=0.091)or flattening index(P=0.987)between two groups.The magnitude of error was-0.37±0.31 D in HAG and-0.04±0.19 D in LAG(P<0.001).Index of success(IOS)was 0.22±0.09 in the HAG and 0.50±0.46 in the LAG(P<0.001).HOAs of most anterior,posterior and total cornea significantly increased after SMILE,especially the spherical aberration and coma.For HAG,the SMILE procedure induced significantly higher anterior,posterior and total cornea horizontal coma and total corneal total HOAs compared with LAG(P<0.001)and these surgically induced HOAs predominantly originated from the anterior surface of the cornea.CONCLUSION:SMILE surgery induces more HOAs and a mild under-correction of astigmatism in eyes with high astigmatism.The increment in HOAs after SMILE is related to preoperative astigmatism.
文摘AIM:To investigate the changes in posterior corneal elevation within 6mo after small incision lenticule extraction(SMILE)surgery for myopia and myopic astigmatism in patients with thin corneas.METHODS:A prospective study included patients with thin corneas(preoperative thinnest corneal thickness ranging from 480 to 520μm)who underwent SMILE for myopia or myopic astigmatism.Corneal topography and posterior corneal elevation were assessed using Pentacam HR at three time points:preoperatively,1mo,and 6mo postoperatively.The measured parameters included thinnest point elevation(PTE),posterior maximal elevation(PME),posterior central elevation(PCE),and 24 additional reference points.RESULTS:A total of 106 eyes from 106 patients(age range:18-34)were included in the study.Uncorrected distance visual acuity(UDVA)improved significantly,with a mean logMAR value of-0.07±0.06 at the final follow-up visit.Measurements of posterior corneal elevation showed no significant changes in most points,hemispheres,and meridians at 6mo postoperatively.Notably,only two points,ΔE_(2mm-45°)andΔE_(2mm-90°),exhibited statistically significant elevation changes:the elevation ofΔE_(2mm-45°)increased from-2.3±4.99 to-1.0±5.9μm(P=0.0037),and that ofΔE_(2mm-90°)increased from-16±7.53 to-15±7.4μm(P=0.016).However,these changes were within the measurement error range of the Pentacam HR(±5μm in a 5 mm area).CONCLUSION:SMILE surgery is a safe and stable procedure for correcting myopia or myopic astigmatism in patients with thin corneas,as evidenced by the stability of posterior corneal elevation.
文摘Sensor nodes cannot directly communicate with the distant unmanned aerial vehicle( UAV) for their low transmission power. Distributed collaborative beamforming from sensor nodes within a cluster is proposed to provide high speed data transmission to the distant UAV. The bit error ratio( BER) closed-form expression of distributed collaborative beamforming transmission with mobile sensor nodes has been derived. Furthermore,based on the theoretical BER analysis and the numerical results,we have analyzed the impacts of nodes 'mobility,number of sensor nodes,transmission power and the elevation angle of UAV on the BER performance of collaborative beamforming. And we come to the following conclusions: the mobility of sensor nodes largely decreases the BER performance; when the position deviation radius is large,incensement in power cannot improve BER anymore; the size of cluster should be bigger than 10 for the purpose of achieving good BER performance in Rayleigh fading channel.
基金supported by the Natural Science Foundation of Sichuan Province(2022NSFSC1767)National Natural Science Foundation of China(32360828)。
文摘The hypothalamic-pituitary-ovarian(HPO)axis represents a central neuroendocrine network essential for reproductive function.Despite its critical role,the intrinsic heterogeneity within the HPO axis across vertebrates and the complex intercellular interactions remain poorly defined.This study provides the first comprehensive,unbiased,cell type-specific molecular profiling of all three components of the HPO axis in adult Lohmann layers and Liangshan Yanying chickens.Within the hypothalamus,pituitary,and ovary,seven,12,and 13 distinct cell types were identified,respectively.Results indicated that the pituitary adenylate cyclase activating polypeptide(PACAP),follicle-stimulating hormone(FSH),and prolactin(PRL)signaling pathways may modulate the synthesis and secretion of gonadotropin-releasing hormone(GnRH),FSH,and luteinizing hormone(LH)within the hypothalamus and pituitary.In the ovary,interactions between granulosa cells and oocytes involved the KIT,CD99,LIFR,FN1,and ANGPTL signaling pathways,which collectively regulate follicular maturation.The SEMA4 signaling pathway emerged as a critical mediator across all three tissues of the HPO axis.Additionally,gene expression analysis revealed that relaxin 3(RLN3),gastrin-releasing peptide(GRP),and cocaine-and amphetamine regulated transcripts(CART,also known as CARTPT)may function as novel endocrine hormones,influencing the HPO axis through autocrine,paracrine,and endocrine pathways.Comparative analyses between Lohmann layers and Liangshan Yanying chickens demonstrated higher expression levels of GRP,RLN3,CARTPT,LHCGR,FSHR,and GRPR in the ovaries of Lohmann layers,potentially contributing to their superior reproductive performance.In conclusion,this study provides a detailed molecular characterization of the HPO axis,offering novel insights into the regulatory mechanisms underlying reproductive biology.
基金Nanning Technology and Innovation Special Program(20204122)and Research Grant for 100 Talents of Guangxi Plan.
文摘Esophageal disease is a common disorder of the digestive system that can severely affect the quality of life andprognosis of patients. Esophageal stenting is an effective treatment that has been widely used in clinical practice.However, esophageal stents of different types and parameters have varying adaptability and effectiveness forpatients, and they need to be individually selected according to the patient’s specific situation. The purposeof this study was to provide a reference for clinical doctors to choose suitable esophageal stents. We used 3Dprinting technology to fabricate esophageal stents with different ratios of thermoplastic polyurethane (TPU)/(Poly-ε-caprolactone) PCL polymer, and established an artificial neural network model that could predict the radial forceof esophageal stents based on the content of TPU, PCL and print parameter. We selected three optimal ratios formechanical performance tests and evaluated the biomechanical effects of different ratios of stents on esophagealimplantation, swallowing, and stent migration processes through finite element numerical simulation and in vitrosimulation tests. The results showed that different ratios of polymer stents had different mechanical properties,affecting the effectiveness of stent expansion treatment and the possibility of postoperative complications of stentimplantation.
基金supported by the National Natural Science Foundation of China(Grant Nos.42175002,42030611,42075013)the Natural Science Foundation of Sichuan,China(Grant No.2023NSFSC0242)the Innovation Team Fund of Southwest Regional Meteorological Center,China Meteorological Administration(Grant No.XNQYCXTD-202202)。
文摘Existing studies contend that latent heating(LH)will replace sensible heating(SH)to become the dominant factor affecting the development of the Tibetan Plateau vortex(TPV)after it moves off the Tibetan Plateau(TP).However,in the process of the TPV moving off the TP requires that the airmass traverse the eastern slope of the Tibetan Plateau(ESTP)where the topography and diabatic heating(DH)conditions rapidly change.How LH gradually replaces SH to become the dominant factor in the development of the TPV over the ESTP is still not very clear.In this paper,an analysis of a typical case of a TPV with a long life history over the ESTP is performed by using multi-sourced meteorological data and model simulations.The results show that SH from the TP surface can change the TPV-associated precipitation distribution by temperature advection after the TPV moves off the TP.The LH can then directly promote the development of the TPV and has a certain guiding effect on the track of the TPV.The SH can control the active area of LH by changing the falling area of the TPV-associated precipitation,so it still plays a key role in the development and tracking of the TPV even though it has moved out of the main body of the TP.
文摘Casing deformation and frac-hit pose significant challenges to the development of deep shale gas in southern Sichuan Basin.By analyzing the mechanism and main control factors of casing deformation and frac-hit,two kinds of risk assessment methods were defined,and the overall prevention and control concept and practice were formulated.The results show that initial stress,pore pressure,fault development and large scale fracturing in local block are the main factors leading to the deformation.The development of fracture through well group and uncontrolled fracturing fluid volume are the main factors leading to pressure channeling.Based on this,the risk classification technology of casing deformation and frac-hit is established,and the dual-optimal,dual-control concept and technology are formed.In terms of the prevention and control of casing deformation,the formation of small-diameter bridge plug fracturing,large section combined fracturing,glass beads cementing,singlewell staggered and platform straddle fracturing mode,dual-dimension controlled and lift fracturing,hyperbolic diagnosis,etc.Frac-hit prevention and control formed pump sequence optimization mode,physical and chemical temporary plugging and other methods.The above technology achieved casing deformation rate decreased from 50.4%to 25.4%,frac-hit rate decreased from 58.6%to 33.9%,and the average well kilometer EUR reached 0.52e0.7 million square meters,an increase of 7.7%compared with the previous research,with remarkable results.
基金supported by grants from the National Science Fund for Distinguished Young Scholars(82225029,to C.H.W.)the Youth Fund of the National Natural Science Foundation of China(82302628,to Y.Y.D.,82301989,to R.R.H.,82301987 to B.Z.and 82402704 to Y.Y.L.)+5 种基金the Original Exploration Program of the National Natural Science Foundation of China(82150102,to C.H.W.)the National Key Research and Development Program of China(2020YFA0710700,to C.H.W.)the Postdoctoral Foundation of China(2022M720658,to Y.Y.D.,and 2022M720659 to R.R.H.)the Sichuan Postdoctoral Innovation Plan(BX202202,to Y.Y.D.)the Postdoctoral Foundation of Sichuan Provincial People’s Hospital(2022BH01,to R.R.H.and 2022BH07,to M.Y.)the Postdoctoral Foundation of Sichuan Province(TB2022086,to R.R.H.,TB2023092,to L.Y.F.).
文摘Proper cellular metabolism in T cells is critical for a productive immune response.However,when dysregulated by intrinsic or extrinsic metabolic factors,T cells may contribute to a wide spectrum of diseases,such as cancers and autoimmune diseases.However,the metabolic regulation of T cells remains incompletely understood.Here,we show that MYO1F is required for human and mouse T-cell activation after TCR stimulation and that T-cell-specific Myo1f knockout mice exhibit an increased tumor burden and attenuated EAE severity due to impaired T-cell activation in vivo.Mechanistically,after TCR stimulation,MYO1F is phosphorylated by LCK at tyrosines 607 and 634,which is critical for glyceraldehyde-3-phosphate dehydrogenase(GAPDH)acetylation at Lys84,86 and 227 mediated byα-TAT1,which is an acetyltransferase,and these processes are important for its activation,cellular glycolysis and thus the effector function of T cells.Importantly,we show that a fusion protein of VAV1-MYO1F,a recurrent peripheral T-cell lymphoma(PTCL)-associated oncogenic protein,promotes hyperacetylation of GAPDH and its activation,which leads to aberrant glycolysis and T-cell proliferation,and that inhibition of the activity of GAPDH significantly limits T-cell activation and proliferation and extends the survival of hVAV1-MYO1F knock-in mice.Moreover,hyperacetylation of GAPDH was confirmed in human PTCL patient samples containing the VAV1-MYO1F gene fusion.Overall,this study revealed not only the mechanisms by which MYO1F regulates T-cell metabolism and VAV1-MYO1F fusion-induced PTCL but also promising therapeutic targets for the treatment of PTCL.
基金supported by the National Natural Science Foundation of China(7084001290924022)the Ph.D.Thesis Innovation and Excellent Foundation of Nanjing University of Aeronautics and Astronautics(2010)
文摘In grey system theory,the studies in the field of grey prediction model are focused on real number sequences,rather than grey number ones.Hereby,a prediction model based on interval grey number sequences is proposed.By mining the geometric features of interval grey number sequences on a two-dimensional surface,all the interval grey numbers are converted into real numbers by means of certain algorithm,and then the prediction model is established based on those real number sequences.The entire process avoids the algebraic operations of grey number,and the prediction problem of interval grey number is usefully solved.Ultimately,through an example＇s program simulation,the validity and practicability of this novel model are verified.
基金supported by Soft Science Foundation of Yongchuan District of Chongqing City(Grant No.YCSTC.2011BE5015)
文摘Objective:To explore the protective effect and its molecular mechanism of apoptosis signalregulating kinase 1(ASK1) inhibitor(GS-459679) on acetaminophen-induced liver injury in mice.Methods:The model of liver injury was established by administration of acetaminophen(APAP)(300 mg/kg,i.p.) on C57BL/6 mice.Forty-eight male C57BL/6 mice were randomly divided into four groups,consisting of control group,GS group(GS-459679,30 mg/kg,i.p.),APAPinduced group,and GS combined with APAP-induced group.For GS combined with APAPinduced group,mice were treated with GS 30 min prior to administration of APAP.After mice were euthanized at 6 h or 12 h.respectively,serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were analyzed,and mRNA levels of TNF- α,IL-6 and IL-1βwere tested.The activity of glutathione(GSH),oxidized GSH(GSSG) and malondialdehyde were quantified.In addition,ASK1,P-ASK1,JNK and P-JNK protein levels were tested in all groups.Results:The ASK1 and P-ASK1 levels were up-regulated in APAP-induced group.Compared to the control group,serum levels of ALT and AST.and mRNA levels of TNF- a,IL-6 and IL-1(3were increased in APAP-induced group.Meanwhile,the levels of MAD and GSSG.and the ratio of GSSG/GSH were higher and the JNK was activatedin APAP-induced group compared with that in control group.However,compared to APAP-induced group,GS combined with APAP-induced group displayed a decrease of protein expression levels of ASK 1,P-ASKI and P-JNK,a reduction of serum levels of ALT and AST,a decrease in TNF- a.IL-6 and IL-1(3 mRNA levels,and a low ration of GSSG/GSH.Conclusions:GS-459679 treatment effectively down-regulates ASK1 and P-ASK 1 expression.Addition of GS-459679 decreases the generation of liver metabolites and inflammatory factors,reduces oxidative stress reaction,inhibits JNK activation,and then protects the responsiveness to APAP-induced liver injury.
基金supported by the National Basic Research Program of China(2020YFA0804000,2020YFC0842000,2020YFA0112200,2021YFC2301703)Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32010100)+6 种基金Special Associate Research Program of the Chinese Academy of Sciences(E1290601)National Natural Science Foundation of China(32122037,81891001,32192411,32100512,U1902215)Collaborative Research Fund of the Chinese Institute for Brain Research,Beijing(2020-NKX-PT-03)CAS Project for Young Scientists in Basic Research(YSBR-013)Young Elite Scientist Sponsorship Program by the China Association for Science and Technology(2020QNRC001)National Resource Center for Non-Human Primates。
文摘Infection with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) causes diverse clinical manifestations and tissue injuries in multiple organs.However, cellular and molecular understanding of SARS-CoV-2 infection-associated pathology and immune defense features in different organs remains incomplete. Here, we profiled approximately 77 000single-nucleus transcriptomes of the lung, liver,kidney, and cerebral cortex in rhesus macaques(Macaca mulatta) infected with SARS-CoV-2 and healthy controls. Integrated analysis of the multiorgan dataset suggested that the liver harbored the strongest global transcriptional alterations. We observed prominent impairment in lung epithelial cells, especially in AT2 and ciliated cells, and evident signs of fibrosis in fibroblasts. These lung injury characteristics are similar to those reported in patients with coronavirus disease 2019(COVID-19).Furthermore, we found suppressed MHC class I/II molecular activity in the lung, inflammatory response in the liver, and activation of the kynurenine pathway,which induced the development of an immunosuppressive microenvironment. Analysis of the kidney dataset highlighted tropism of tubule cells to SARS-CoV-2, and we found membranous nephropathy(an autoimmune disease) caused by podocyte dysregulation. In addition, we identified the pathological states of astrocytes and oligodendrocytes in the cerebral cortex, providing molecular insights into COVID-19-related neurological implications. Overall, our multi-organ single-nucleus transcriptomic survey of SARS-CoV-2-infected rhesus macaques broadens our understanding of disease features and antiviral immune defects caused by SARS-CoV-2 infection,which may facilitate the development of therapeutic interventions for COVID-19.
基金Supported by National Natural Science Foundation of China(No.81200661)Nature Science Foundation of Hubei Province(No.2010CDB09802)Wuhan Chenguang Plan Grant(No.201150431124)
文摘AIM:To investigate the effect of amniotic membrane covering(AMC) on the healing of cornea epithelium and visual acuity for fungal keratitis after debridement.METHODS:Twenty fungal keratitis patients were divided into two groups randomly, the AMC group and the control group, ten patients each group. Both debridement of the infected cornea tissue and standard anti-fungus drugs treatments were given to every patients, monolayer amniotic membrane were sutured to the surface of the entire cornea and bulbar conjunctiva with 10-0 nylon suture for patients in the AMC group.The diameter of the ulcer was determined with slit lamp microscope and the depth of the infiltration was determined with anterior segment optical coherence tomography. Uncorrected visual acuity(UCVA) was tested before surgery and three month after healing of the epithelial layer. The healing time of the cornea epithelium, visual acuity(VA) was compared between the two groups using t- test.RESULTS:There was no statistical difference of the diameter of the ulcer, depth of the infiltration, height of the hypopyon and VA between the two groups beforesurgery(P 】0.05). The average healing time of the AMC group was 6.89 ±2.98 d, which was statistically shorter than that of the control group(10.23±2.78d)(P 【0.05).The average UCVA of the AMC group was 0.138 ±0.083,which was statistically better than that of the control group(0.053±0.068)(P 【0.05).CONCLUSION:AMC surgery could promote healing of cornea epithelium after debridement for fungal keratitis and lead to better VA outcome.
基金supported by the National Key Research and Development Program of China(No.2017YFA0208000)the National Natural Science Foundation of China(Nos.21925401,21904033,21675120)Changsha Municipal Science and Technology Projects,China(No.kq1901030)。
文摘Bladder cancer is the most common malignant tumor in the urinary system,with high morbidity,mortality and recurrence after surgery.However,current bladder cancer urine diagnosis methods are limited by the low accuracy and specificity due to the low abundance of bladder cancer biomarkers in the urine with complex biological environments.Herein,we present a high stability indium gallium zinc oxide field effect transistor(IGZO-FET)biosensor for efficient identification of bladder cancer biomarkers from human urine samples.The recognition molecular functionalized IGZO-FET biosensor exhibits stable electronic and sensing performance due to the large-area fabrication of IGZO thin-film FET.Owing to the excellent electrical performance of IGZO-FET,the IGZO-FET biosensor exhibits high sensitivity and extremely low detection limit(2.7 amol/L)towards bladder cancer biomarkers.The IGZO-FET biosensor is also able to directly detect bladder tumor biomarker in human urine with high sensitivity and specificity,and could differentiate bladder cancer patients’urine samples from healthy donors effectively.These results indicate that our designed high-performance biosensor shows great potential in the application of portable digital bladder cancer diagnosis devices.
