Tetrandrine （TET）, a traditional Chinese medicine, exerts remarkable anticancer activity on various cancer cells. However, little is known about the effect of TET on human prostate cancer cells, and the mechanism of...Tetrandrine （TET）, a traditional Chinese medicine, exerts remarkable anticancer activity on various cancer cells. However, little is known about the effect of TET on human prostate cancer cells, and the mechanism of function of TET on prostate cancer has not yet been elucidated. To investigate the effects of TET on the suppression of proliferation, induction of apoptosis, and inhibition of migration and invasion in human prostate cancer cell lines, DU145 and PC-3. Inhibition of growth was determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay and clone formation assay, and flow cytometry analysis was performed to detect the induction of apoptosis. Activation of poly （ADP-ribose） polymerase, caspase-3, Akt, phospho-Akt, Bcl-2, and Bax was analyzed by Western blotting. Wound healing assay and transwell migration assay were used to evaluate the effect of TET on migration and invasion of cancer cells. TET inhibited the growth of DU145 and PC-3 cells in a dose- and time-dependent manner. Cell cloning was inhibited in the presence of TET in DU145 and PC-3 cells. TET suppressed the migration of DU145 and PC-3 cells. Transwell invasion assay showed that TET significantly weakened invasion capacity of DU 145 and PC-3 cells. TET exhibited strong inhibitory effect on proliferation, migration, and invasion of prostate cancer cells. In addition, TET induced apoptosis in a dose-dependent manner by activating the caspase cascade and inhibiting phosphoinositide 3-kinase-Akt signal pathway. The accumulating evidence suggests that TET could be a potential therapeutic candidate against prostate cancer in a clinical setting.展开更多
The transmission medium of natural gas gathering and transportation pipelines usually contains cor-rosive gases,which will cause serious corrosion on the inner wall of the pipelines when they coexist with water.Theref...The transmission medium of natural gas gathering and transportation pipelines usually contains cor-rosive gases,which will cause serious corrosion on the inner wall of the pipelines when they coexist with water.Therefore,it is necessary to add corrosion inhibitor to form a protective film to protect the pipeline.The distribution of corrosion inhibitors in a gathering and transportation pipeline in Moxi gas field was studied by combining experiment and simulation.The Pearson function was used to calculate the experimental and simulation results,and the correlation was more than 80%,indicating a high degree of agreement.The simulation results show that:①The larger the pipe angle,filling speed and gas flow rate,the smaller the particle size,the better the distribution of corrosion inhibitor particles in the pipe.The filling amount will affect the concentration,but the distribution trend is unchanged;②A method to determine the filling mode based on the loss was proposed,and for this pipeline,the loss of corrosion inhibitor was determined to be 5.31×10^(-3) kg/s,and the flling amount was recommended to be adjusted to 2o L/h,which has certain guiding significance for the actual flling strategy of pipeline corrosion inhibitor.展开更多
This study aimed to investigate the correlations among androgen receptor （AR） CAG repeat polymorphism, sex hormones and penile length in healthy Chinese young adult men. Two hundred and fifty-three healthy men （age...This study aimed to investigate the correlations among androgen receptor （AR） CAG repeat polymorphism, sex hormones and penile length in healthy Chinese young adult men. Two hundred and fifty-three healthy men （aged 22.8 ± 3.1years） were enrolled. The individuals were grouped as CAG short （CAGs） if they harbored repeat length of 〈20 or as CAG long （CAGL） if their CAG repeat length was 〉20. Body height/weight, penile length and other parameters were examined and recorded by the specified physicians; CAG repeat polymorphism was determined by the polymerase chain reaction （PCR） method; and the serum levels of the sex hormones were detected by radioimmunoassay. Student＇s t-test or linear regression analysis was used to assess the associations among AR CAG repeat polymorphism, sex hormones and penile length. This investigation showed that the serum total testosterone （T） level was positively associated with the AR CAG repeat length （P = 0.01）; whereas, no significant correlation of T or AR CAG repeat polymorphism with the penile length was found （P = 0.593）. Interestingly, an inverse association was observed between serum prolactin （PRL） levels and penile length by linear regression analyses （β = -0.024, P = 0.039, 95% confidence interval （CI）： -0.047, 0）. Collectively, this study provides the first evidence that serum PRL, but not T or AR CAG repeat polymorphism, is correlated with penile length in the Han adult population from northwestern China.展开更多
The widespread commercial application of direct formate fuel cell(DFFC)is limited by the lack of efficient electrocatalysts for the formate oxidation reaction(FOR).AgPdCo hollow nanospheres(H-NSs)with jagged surfaces ...The widespread commercial application of direct formate fuel cell(DFFC)is limited by the lack of efficient electrocatalysts for the formate oxidation reaction(FOR).AgPdCo hollow nanospheres(H-NSs)with jagged surfaces are successfully synthesized via a facile method involving the wet-chemical synthesis of AgPdCo nanospheres(NSs)and galvanic replacement reaction between Pd salt and AgPdCo NSs.Surpassing Ag_(30)Pd_(69)Co_(1) NSs and most of previously reported electrocatalysts,Ag_(9)Pd_(90)Co_(1) H-NSs exhibit extremely high FOR activity with a peak current density of 3.08 A·mg_(Pd)^(−1).Apart from the competitive activity,Ag_(9)Pd_(90)Co_(1) H-NSs show greatly improved chronoamperometric and cycling stability,whereby the current density retains about 0.24 A·mg_(Pd)^(−1) after 3,600 s electrocatalysis and the mass activity maintains 54.06%of the initial value after 500 cycles.The unique hollow nanosphere and synergistic effect are responsible for the enhanced activity and stability.This study will provide new clues for the development of outstanding electrocatalysts.展开更多
文摘Tetrandrine （TET）, a traditional Chinese medicine, exerts remarkable anticancer activity on various cancer cells. However, little is known about the effect of TET on human prostate cancer cells, and the mechanism of function of TET on prostate cancer has not yet been elucidated. To investigate the effects of TET on the suppression of proliferation, induction of apoptosis, and inhibition of migration and invasion in human prostate cancer cell lines, DU145 and PC-3. Inhibition of growth was determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay and clone formation assay, and flow cytometry analysis was performed to detect the induction of apoptosis. Activation of poly （ADP-ribose） polymerase, caspase-3, Akt, phospho-Akt, Bcl-2, and Bax was analyzed by Western blotting. Wound healing assay and transwell migration assay were used to evaluate the effect of TET on migration and invasion of cancer cells. TET inhibited the growth of DU145 and PC-3 cells in a dose- and time-dependent manner. Cell cloning was inhibited in the presence of TET in DU145 and PC-3 cells. TET suppressed the migration of DU145 and PC-3 cells. Transwell invasion assay showed that TET significantly weakened invasion capacity of DU 145 and PC-3 cells. TET exhibited strong inhibitory effect on proliferation, migration, and invasion of prostate cancer cells. In addition, TET induced apoptosis in a dose-dependent manner by activating the caspase cascade and inhibiting phosphoinositide 3-kinase-Akt signal pathway. The accumulating evidence suggests that TET could be a potential therapeutic candidate against prostate cancer in a clinical setting.
基金supported by the Sichuan Natural Science Foundation(2023NSFSC0422)the Petrochina's"14th Five-Year plan"Project(2021Dj2804).
文摘The transmission medium of natural gas gathering and transportation pipelines usually contains cor-rosive gases,which will cause serious corrosion on the inner wall of the pipelines when they coexist with water.Therefore,it is necessary to add corrosion inhibitor to form a protective film to protect the pipeline.The distribution of corrosion inhibitors in a gathering and transportation pipeline in Moxi gas field was studied by combining experiment and simulation.The Pearson function was used to calculate the experimental and simulation results,and the correlation was more than 80%,indicating a high degree of agreement.The simulation results show that:①The larger the pipe angle,filling speed and gas flow rate,the smaller the particle size,the better the distribution of corrosion inhibitor particles in the pipe.The filling amount will affect the concentration,but the distribution trend is unchanged;②A method to determine the filling mode based on the loss was proposed,and for this pipeline,the loss of corrosion inhibitor was determined to be 5.31×10^(-3) kg/s,and the flling amount was recommended to be adjusted to 2o L/h,which has certain guiding significance for the actual flling strategy of pipeline corrosion inhibitor.
文摘This study aimed to investigate the correlations among androgen receptor （AR） CAG repeat polymorphism, sex hormones and penile length in healthy Chinese young adult men. Two hundred and fifty-three healthy men （aged 22.8 ± 3.1years） were enrolled. The individuals were grouped as CAG short （CAGs） if they harbored repeat length of 〈20 or as CAG long （CAGL） if their CAG repeat length was 〉20. Body height/weight, penile length and other parameters were examined and recorded by the specified physicians; CAG repeat polymorphism was determined by the polymerase chain reaction （PCR） method; and the serum levels of the sex hormones were detected by radioimmunoassay. Student＇s t-test or linear regression analysis was used to assess the associations among AR CAG repeat polymorphism, sex hormones and penile length. This investigation showed that the serum total testosterone （T） level was positively associated with the AR CAG repeat length （P = 0.01）; whereas, no significant correlation of T or AR CAG repeat polymorphism with the penile length was found （P = 0.593）. Interestingly, an inverse association was observed between serum prolactin （PRL） levels and penile length by linear regression analyses （β = -0.024, P = 0.039, 95% confidence interval （CI）： -0.047, 0）. Collectively, this study provides the first evidence that serum PRL, but not T or AR CAG repeat polymorphism, is correlated with penile length in the Han adult population from northwestern China.
基金This work was supported by the National Natural Science Foundation of China(Nos.51874243,51271148,and 50971100)the Research Fund of State Key Laboratory of Solidification Processing(NPU),China(No.2020-TS-02)+1 种基金the Project of Transformation of Scientific and Technological Achievements of NWPU(No.19-2017)the Open Fund of State Key Laboratory of Advanced Technology for Material Synthesis and Processing(Wuhan University of Technology,No.2018KF-18).
文摘The widespread commercial application of direct formate fuel cell(DFFC)is limited by the lack of efficient electrocatalysts for the formate oxidation reaction(FOR).AgPdCo hollow nanospheres(H-NSs)with jagged surfaces are successfully synthesized via a facile method involving the wet-chemical synthesis of AgPdCo nanospheres(NSs)and galvanic replacement reaction between Pd salt and AgPdCo NSs.Surpassing Ag_(30)Pd_(69)Co_(1) NSs and most of previously reported electrocatalysts,Ag_(9)Pd_(90)Co_(1) H-NSs exhibit extremely high FOR activity with a peak current density of 3.08 A·mg_(Pd)^(−1).Apart from the competitive activity,Ag_(9)Pd_(90)Co_(1) H-NSs show greatly improved chronoamperometric and cycling stability,whereby the current density retains about 0.24 A·mg_(Pd)^(−1) after 3,600 s electrocatalysis and the mass activity maintains 54.06%of the initial value after 500 cycles.The unique hollow nanosphere and synergistic effect are responsible for the enhanced activity and stability.This study will provide new clues for the development of outstanding electrocatalysts.
