The jasmonate signaling pathway coordinates plant defenses and growth,thereby enhancing fitness in changing conditions.Jasmonate-mediated responses are triggered by the recognition of external signals via pattern reco...The jasmonate signaling pathway coordinates plant defenses and growth,thereby enhancing fitness in changing conditions.Jasmonate-mediated responses are triggered by the recognition of external signals via pattern recognition receptors(PRRs)located on the cell membrane.Following signal perception,cells rapidly activate jasmonic acid(JA)biosynthesis,resulting in the accumulation of the bioactive jasmonate,jasmonoyl-isoleucine(JA-Ile).In the nucleus,the coronatine insensitive 1-jasmonate-ZIMdomain(COI1-JAZ)complex recognizes JA-Ile and triggers JAZ ubiquitination and proteasomal degradation.Consequently,transcription factors(e.g.,MYC2)bound by JAZ are released,enabling the activation and amplification of JA responses.In parallel to this activation,feedback regulation orchestrated by transcription factors terminates transcription,preventing overcommitment to JA signaling.In this review,we summarize recent advances in understanding JA signaling,emphasizing the connection between PRR activation and JA biosynthesis,and the feedback regulatory mechanisms that ensure precision and robustness of the JA signaling pathway.Finally,we discuss how these mechanistic insights can be leveraged to optimize JA signaling for crop genetic improvement.展开更多
Dear Editor,The doubled haploid(DH)breeding methodology,which combines in vivo haploid induction,selection,and chromosome doubling to stabilize recombinant haplotypes within two generations,has revolutionized conventi...Dear Editor,The doubled haploid(DH)breeding methodology,which combines in vivo haploid induction,selection,and chromosome doubling to stabilize recombinant haplotypes within two generations,has revolutionized conventional breeding by accelerating genetic gains.Initially pioneered in maize,this technology has been expanded to several major crops,including rice,wheat,tomato,soybean,and rapeseed(Zhong et al.,2022;Qu et al.,2024;Xia et al.,2024).However,haploid induction rates(HIRs)in dicots,such as tomato,remain remarkably low,and efficient approaches for identifying high-HIR inducers(HHIs)are lacking,creating a bottleneck for the broader implementation of DH breeding in these crops.Furthermore,rapid and reliable haploid identification(HID)for subsequent chromosome doubling remains a technical challenge.To overcome these limitations,we established a new strategy based on anthocyanin as a marker that significantly advances DH efficiency in tomato breeding.Specifically,we developed an anthocyanin-deficient male-sterile tester line that enables precise screening of HHIs,and an anthocyanin over-accumulating transgenic haploid inducer that facilitates rapid and accurate HID.展开更多
基金supported by the National Natural Science Foundation of China(grants 32402586,32372705)Beijing Rural Revitalization Agricultural Science and Technology Project(NY2401080000)+2 种基金the 2115 Talent Development Program of China Agricultural University,Chinese Universities Scientific Fund(15055005)the Open Project of National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops,Hainan Provincial Natural Science Foundation of China(324CXTD426)Science and Technology Special Fund of Hainan Province(ZDYF2025XDNY082).
文摘The jasmonate signaling pathway coordinates plant defenses and growth,thereby enhancing fitness in changing conditions.Jasmonate-mediated responses are triggered by the recognition of external signals via pattern recognition receptors(PRRs)located on the cell membrane.Following signal perception,cells rapidly activate jasmonic acid(JA)biosynthesis,resulting in the accumulation of the bioactive jasmonate,jasmonoyl-isoleucine(JA-Ile).In the nucleus,the coronatine insensitive 1-jasmonate-ZIMdomain(COI1-JAZ)complex recognizes JA-Ile and triggers JAZ ubiquitination and proteasomal degradation.Consequently,transcription factors(e.g.,MYC2)bound by JAZ are released,enabling the activation and amplification of JA responses.In parallel to this activation,feedback regulation orchestrated by transcription factors terminates transcription,preventing overcommitment to JA signaling.In this review,we summarize recent advances in understanding JA signaling,emphasizing the connection between PRR activation and JA biosynthesis,and the feedback regulatory mechanisms that ensure precision and robustness of the JA signaling pathway.Finally,we discuss how these mechanistic insights can be leveraged to optimize JA signaling for crop genetic improvement.
基金supported by grants from the Beijing Municipal Bureau of Agriculture and Rural Affairs(grant no.NY2401080000)the Hainan Provincial Natural Science Foundation of China(grant no.324CXTD426)+2 种基金the Beijing Academy of Agriculture and Forestry Sciences(grant nos.KYCX202303 and XTCX202303)the Science and Technology Special Fund of Hainan Province(grant no.ZDYF2025XDNY082)the National Natural Science Foundation of China(grant nos.32402586 and 32372705).
文摘Dear Editor,The doubled haploid(DH)breeding methodology,which combines in vivo haploid induction,selection,and chromosome doubling to stabilize recombinant haplotypes within two generations,has revolutionized conventional breeding by accelerating genetic gains.Initially pioneered in maize,this technology has been expanded to several major crops,including rice,wheat,tomato,soybean,and rapeseed(Zhong et al.,2022;Qu et al.,2024;Xia et al.,2024).However,haploid induction rates(HIRs)in dicots,such as tomato,remain remarkably low,and efficient approaches for identifying high-HIR inducers(HHIs)are lacking,creating a bottleneck for the broader implementation of DH breeding in these crops.Furthermore,rapid and reliable haploid identification(HID)for subsequent chromosome doubling remains a technical challenge.To overcome these limitations,we established a new strategy based on anthocyanin as a marker that significantly advances DH efficiency in tomato breeding.Specifically,we developed an anthocyanin-deficient male-sterile tester line that enables precise screening of HHIs,and an anthocyanin over-accumulating transgenic haploid inducer that facilitates rapid and accurate HID.
