Colorectal cancer(CRC)is a digestive tract malignancy with the highest global incidence and mortality rate.In China,the number of patients with colorectal cancer has been increasing annually.In 2020,the numbers of new...Colorectal cancer(CRC)is a digestive tract malignancy with the highest global incidence and mortality rate.In China,the number of patients with colorectal cancer has been increasing annually.In 2020,the numbers of new cases and cancer deaths were 560,000 and 290,000,respectively,ranking second among new cancer cases and fifth among cancer deaths in China.Treatment of colorectal cancer involves a combined regimen of surgery supplemented with radiotherapy,chemotherapy,targeted therapy,and immunotherapy.This article comprehensively analyzed the classification and function of natural killer(NK)cells,their development and metabolic processes,expression and effector mechanisms,their effects on colorectal cancer,and the development prospects of using NK cells to treat colorectal cancer.Here,we comprehensively review preclinical and clinical investigations exploring the therapeutic effects and mechanisms of NK cell-based immunotherapy against CRC.This approach not only leverages NK cells’unique abilities to recognize and eliminate tumor cells but also overcomes the immunosuppressive tumor microenvironment in advanced CRC,providing a promising strategy for future therapeutic development.展开更多
Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast...Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast cancer cells in vitro,which can provide experimental ideas and methods for the establishment of a living tumor tissue cell bank.Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who needed surgical treatment in our hospital.Cells were isolated and used to establish successful cell culture.Cell activity and cell purity were measured before liquid nitrogen cryopreservation.Results(1)At the initial culture stage,cells grew with adherence.Cell multiplication could be seen after the cell medium was exchanged three times.Cell viability was above 86%,while the viability of the target cells was above 75%,as detected by hematoxylin and eosin(HE)staining.(2)The number of breast cancer cells decreased,while the number of fibroblasts increased after five rounds of passage.(3)The success rate was 73.08%,which did not include polluted cells and those that were not successfully cryopreserved.Conclusion(1)breast cancer cells could be selected from primary culture in vitro through an appropriate method.(2)Exchange of the cell medium and further cell passage improved cell multiplication.(3)The experimental results could be monitored using trypan blue and HE staining.(4)The success of breast cancer cell culture in vitro could be used as a reference for other cell culture,so as to establish a tumor tissue cell bank.展开更多
基金supported by the Inner Mongolia Autonomous Region Science and Technology Plan Project (No.2022YFSH0055)Public Hospital Scientific Research Joint Fund Project (No.2023GLLH0106)The Start-up Fund for Doctoral Scientific Research of Inner Mongolia Autonomous Region People's Hospital(No.2024BS04)
文摘Colorectal cancer(CRC)is a digestive tract malignancy with the highest global incidence and mortality rate.In China,the number of patients with colorectal cancer has been increasing annually.In 2020,the numbers of new cases and cancer deaths were 560,000 and 290,000,respectively,ranking second among new cancer cases and fifth among cancer deaths in China.Treatment of colorectal cancer involves a combined regimen of surgery supplemented with radiotherapy,chemotherapy,targeted therapy,and immunotherapy.This article comprehensively analyzed the classification and function of natural killer(NK)cells,their development and metabolic processes,expression and effector mechanisms,their effects on colorectal cancer,and the development prospects of using NK cells to treat colorectal cancer.Here,we comprehensively review preclinical and clinical investigations exploring the therapeutic effects and mechanisms of NK cell-based immunotherapy against CRC.This approach not only leverages NK cells’unique abilities to recognize and eliminate tumor cells but also overcomes the immunosuppressive tumor microenvironment in advanced CRC,providing a promising strategy for future therapeutic development.
基金a grant from the Science Foundation of Inner Mongolia Autonomous Region People’s Hospital(No.2016015).
文摘Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast cancer cells in vitro,which can provide experimental ideas and methods for the establishment of a living tumor tissue cell bank.Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who needed surgical treatment in our hospital.Cells were isolated and used to establish successful cell culture.Cell activity and cell purity were measured before liquid nitrogen cryopreservation.Results(1)At the initial culture stage,cells grew with adherence.Cell multiplication could be seen after the cell medium was exchanged three times.Cell viability was above 86%,while the viability of the target cells was above 75%,as detected by hematoxylin and eosin(HE)staining.(2)The number of breast cancer cells decreased,while the number of fibroblasts increased after five rounds of passage.(3)The success rate was 73.08%,which did not include polluted cells and those that were not successfully cryopreserved.Conclusion(1)breast cancer cells could be selected from primary culture in vitro through an appropriate method.(2)Exchange of the cell medium and further cell passage improved cell multiplication.(3)The experimental results could be monitored using trypan blue and HE staining.(4)The success of breast cancer cell culture in vitro could be used as a reference for other cell culture,so as to establish a tumor tissue cell bank.
