AIM To study the effect of selenium on peripheral blood mononuclear cell (PBMC) membrane fluidity and immune function in patients with chronic hepatitis.METHODS: PBMCs were pretreated with selenium (1.156x 10.7 mol/L)...AIM To study the effect of selenium on peripheral blood mononuclear cell (PBMC) membrane fluidity and immune function in patients with chronic hepatitis.METHODS: PBMCs were pretreated with selenium (1.156x 10.7 mol/L) for 6 h in vitro or extracted directly from patients after administration of selenium-yeast continuously for 8-12 wk (200 pg/d), and then exposed to Con-A for 48 h. The membrane fluidity, interleukin-2 (IL-2) production and interleukin-2 receptor (IL-2R) expression in PBMCs and malondialdehyde (MDA) concentration in medium and lipid peroxide (LPO) in plasma were determined.RESULTS: The PBMC membrane fluidity, IL-2 production and IL-2R expression in patients with chronic hepatitis were significantly lower than those in healthy blood donators (particle adhesive degree R, 0.17±0.01 vs 0.14±0.01,P<0.01; IL-2, 40.26±9.55 vs72.96±11.36, P<0.01, IL-2R,31.05±5.09 vs 60.58±10.56, P<0.01), and the MDA concentration in medium in patients with chronic hepatitis was significantly higher than that in healthy blood donators (1.44±0.08 vs0.93±0.08, P<0.01). Both in vitro and in vivo administration of selenium could reverse the above parameters.CONCLUSION: Supplement of selenium can suppress lipid peroxidation, and improve PBMC membrane fluidity and immune function in patients with chronic hepatitis.展开更多
The PHBV (beta-hydroxybutyrate-co-beta-hydroxyvalerate) foams as oil sorbentmaterials, which were prepared by salting out method based on PHBV and chloroform additive and theiroil pick-up abilities were investigated. ...The PHBV (beta-hydroxybutyrate-co-beta-hydroxyvalerate) foams as oil sorbentmaterials, which were prepared by salting out method based on PHBV and chloroform additive and theiroil pick-up abilities were investigated. And the oil pick-up abilities of different PHBV foams asoil sorbent materials were compared. The results show that with the amount of chloroform additiveand sodium chloride increasing, the oil pick-up rates, the oil-keeping rates and the second oilpick-up rates of PHBV foams can increase to different extent. When the amount of sodium chloride was92 percent, the ratio of PHBV to chloroform was 1 (g): 14 (mL), the oil pick-up ability of the PHBVfoam was optimal. And their oil pick-up ratios can reach 25.51 (26 deg C) and 27.51 g/g (17 deg C)in raw oil, which were 4-5 times more than those of PHBV powder. In addition, the structure-modifiedPHBV as oil sorbent materials also has some advantages, such as three-dimension structure,bio-degradability and good oil pick-up ability, so this kind of green oil sorbent materials willhave great perspective in the future.展开更多
Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto t...Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto the microtubule plus ends and orchestrates micro- tubule plus-end dynamics during cell division. Here we show that TIP150 cooperates with MCAK to govern entosis via a regulatory cir- cuitry that involves Aurora A-mediated phosphorylation of MCAK. Our biochemical analyses show that MCAK forms an intra-molecular association, which is essential for TIP150 binding. Interestingly, Aurora A-mediated phosphorylation of MCAK modulates its intra-mo- lecular association, which perturbs the MCAK-TI P150 interaction in vitro and inhibits entosis in vivo. To probe if MCAK-TIP150 inter- action regulates microtubule plasticity to affect the mechanical properties of ceUs during entosis, we used an optical trap to measure the mechanical rigidity of live MCF7 ceils. We find that the MCAK cooperates with TIP150 to promote microtubule dynamics and modulate the mechanical rigidity of the cells during entosis. Our results show that a dynamic interaction of MCAK-TI P150 orchestrated by Aurora A-mediated phosphorylation governs entosis via regulating microtubule plus-end dynamics and cell rigidity. These data reveal a previously unknown mechanism of Aurora A regulation in the control of microtubule plasticity during ceU-in-ceU pro- cesses.展开更多
文摘AIM To study the effect of selenium on peripheral blood mononuclear cell (PBMC) membrane fluidity and immune function in patients with chronic hepatitis.METHODS: PBMCs were pretreated with selenium (1.156x 10.7 mol/L) for 6 h in vitro or extracted directly from patients after administration of selenium-yeast continuously for 8-12 wk (200 pg/d), and then exposed to Con-A for 48 h. The membrane fluidity, interleukin-2 (IL-2) production and interleukin-2 receptor (IL-2R) expression in PBMCs and malondialdehyde (MDA) concentration in medium and lipid peroxide (LPO) in plasma were determined.RESULTS: The PBMC membrane fluidity, IL-2 production and IL-2R expression in patients with chronic hepatitis were significantly lower than those in healthy blood donators (particle adhesive degree R, 0.17±0.01 vs 0.14±0.01,P<0.01; IL-2, 40.26±9.55 vs72.96±11.36, P<0.01, IL-2R,31.05±5.09 vs 60.58±10.56, P<0.01), and the MDA concentration in medium in patients with chronic hepatitis was significantly higher than that in healthy blood donators (1.44±0.08 vs0.93±0.08, P<0.01). Both in vitro and in vivo administration of selenium could reverse the above parameters.CONCLUSION: Supplement of selenium can suppress lipid peroxidation, and improve PBMC membrane fluidity and immune function in patients with chronic hepatitis.
文摘The PHBV (beta-hydroxybutyrate-co-beta-hydroxyvalerate) foams as oil sorbentmaterials, which were prepared by salting out method based on PHBV and chloroform additive and theiroil pick-up abilities were investigated. And the oil pick-up abilities of different PHBV foams asoil sorbent materials were compared. The results show that with the amount of chloroform additiveand sodium chloride increasing, the oil pick-up rates, the oil-keeping rates and the second oilpick-up rates of PHBV foams can increase to different extent. When the amount of sodium chloride was92 percent, the ratio of PHBV to chloroform was 1 (g): 14 (mL), the oil pick-up ability of the PHBVfoam was optimal. And their oil pick-up ratios can reach 25.51 (26 deg C) and 27.51 g/g (17 deg C)in raw oil, which were 4-5 times more than those of PHBV powder. In addition, the structure-modifiedPHBV as oil sorbent materials also has some advantages, such as three-dimension structure,bio-degradability and good oil pick-up ability, so this kind of green oil sorbent materials willhave great perspective in the future.
文摘Entosis, a ceU-in-ceU process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TI P150 facilitates the loading of MCAK onto the microtubule plus ends and orchestrates micro- tubule plus-end dynamics during cell division. Here we show that TIP150 cooperates with MCAK to govern entosis via a regulatory cir- cuitry that involves Aurora A-mediated phosphorylation of MCAK. Our biochemical analyses show that MCAK forms an intra-molecular association, which is essential for TIP150 binding. Interestingly, Aurora A-mediated phosphorylation of MCAK modulates its intra-mo- lecular association, which perturbs the MCAK-TI P150 interaction in vitro and inhibits entosis in vivo. To probe if MCAK-TIP150 inter- action regulates microtubule plasticity to affect the mechanical properties of ceUs during entosis, we used an optical trap to measure the mechanical rigidity of live MCF7 ceils. We find that the MCAK cooperates with TIP150 to promote microtubule dynamics and modulate the mechanical rigidity of the cells during entosis. Our results show that a dynamic interaction of MCAK-TI P150 orchestrated by Aurora A-mediated phosphorylation governs entosis via regulating microtubule plus-end dynamics and cell rigidity. These data reveal a previously unknown mechanism of Aurora A regulation in the control of microtubule plasticity during ceU-in-ceU pro- cesses.
