Three types of MCM-41 absorbents, namely, Al^（3＋）-MCM-41, La^（3＋）-MCM-41, and Zn^（2＋）-MCM-41, were prepared through amine grafting, phosphonate modification, and metal ion chelation and characterized by Fouri...Three types of MCM-41 absorbents, namely, Al^（3＋）-MCM-41, La^（3＋）-MCM-41, and Zn^（2＋）-MCM-41, were prepared through amine grafting, phosphonate modification, and metal ion chelation and characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and N2adsorption-desorption analysis. Results revealed that functionalized MCM-41 maintains the original structure of the molecular sieve and that the pore diameter and surface area are reduced compared with those of pure MCM-41. The adsorption behavior of DNA molecules on the surface of the modified molecular sieves was discussed according to the hard-soft acid-base（HSAB） principle. Experimental results showed that DNA purification could be effectively carried out on functionalized MCM-41 and that DNA is easily released by3-4 molL^（-1）NaCl solution. This study could be used as a general platform for future work on DNA adsorption and enrichment.展开更多
基金supported by the National Natural Science Foundation of China (No. 21205070)the Outstanding Youth Scientist Research Foundation of Shandong Province (No. 2007BS02003)
文摘Three types of MCM-41 absorbents, namely, Al^（3＋）-MCM-41, La^（3＋）-MCM-41, and Zn^（2＋）-MCM-41, were prepared through amine grafting, phosphonate modification, and metal ion chelation and characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and N2adsorption-desorption analysis. Results revealed that functionalized MCM-41 maintains the original structure of the molecular sieve and that the pore diameter and surface area are reduced compared with those of pure MCM-41. The adsorption behavior of DNA molecules on the surface of the modified molecular sieves was discussed according to the hard-soft acid-base（HSAB） principle. Experimental results showed that DNA purification could be effectively carried out on functionalized MCM-41 and that DNA is easily released by3-4 molL^（-1）NaCl solution. This study could be used as a general platform for future work on DNA adsorption and enrichment.
