Objectives This study aimed to examine the associations between depression,resilience,and fatigue in patients with multivessel coronary disease and verify their causal relationships.Methods Between October 2023 and Ju...Objectives This study aimed to examine the associations between depression,resilience,and fatigue in patients with multivessel coronary disease and verify their causal relationships.Methods Between October 2023 and June 2024,316 patients with multivessel coronary disease were recruited from three tertiary hospitals in Tangshan,China.The Patient Health Questionnaire,Connor-Davidson Resilience Scale,and the Multidimensional Fatigue Inventory were administered to the patients on the third day of admission(T1),one month after discharge(T2),and three months after discharge(T3).Pearson correlation analysis was conducted to examine the relationships among depression,resilience,and fatigue in patients with multivessel coronary disease,and cross-lagged analysis to explore the temporal causal relationships.Results In patients with multivessel coronary disease,levels of depression and fatigue decreased from T1 to T3,while resilience scores increased during the same period.The correlation analysis revealed significant relationships among depression,resilience,and fatigue at T1,T2,and T3(P<0.01).The autoregressive paths indicated high stability over time for depression,medium stability for resilience,and low stability for fatigue.Cross-lagged paths demonstrated that depression at T1 significantly predicted fatigue at T2(β=0.461,P<0.001),and depression at T2 significantly predicted fatigue at T3(β=0.957,P<0.001).And resilience at T1 significantly predicted fatigue at T2(β=−0.271,P<0.001),and resilience at T2 significantly predicted fatigue at T3(β=−0.176,P<0.001).Additionally,resilience had a moderating effect on the relationship between depression and fatigue(β=−0.760,P<0.001).Conclusions Our study confirmed that depression and resilience predicted fatigue in patients with multivessel coronary disease.To prevent and mitigate fatigue,alleviating depressive symptoms and enhancing resilience levels in patients at an early stage is essential.展开更多
Increasing evidence suggests that white matter disorders based on myelin sheath impairment may underlie the neuropathological changes in schizophrenia.But it is unknown whether enhancing remyelination is a beneficial ...Increasing evidence suggests that white matter disorders based on myelin sheath impairment may underlie the neuropathological changes in schizophrenia.But it is unknown whether enhancing remyelination is a beneficial approach to schizophrenia.To investigate this hypothesis,we used clemastine,an FDA-approved drug with high potency in promoting oligodendroglial differentiation and myelination,on a cuprizone-induced mouse model of demyelination.The mice exposed to cuprizone（0.2%in chow） for 6 weeks displayed schizophrenia-like behavioral changes,including decreased exploration of the center in the open field test and increased entries into the arms of the Y-maze,as well as evident demyelination in the cortex and corpus callosum.Clemastine treatment was initiated upon cuprizone withdrawal at 10 mg/kg per day for3 weeks.As expected,myelin repair was greatly enhanced in the demyelinated regions with increased mature oligodendrocytes（APC-positive） and myelin basic protein.More importantly,the clemastine treatment rescued the schizophrenia-like behavioral changes in the open field test and the Y-maze compared to vehicle,suggesting a beneficial effect via promoting myelin repair.Our findings indicate that enhancing remyelination may be a potential therapy for schizophrenia.展开更多
The rapid elongation of rice(Oryza sativa)coleoptile is pivotal for the plant plumule to evade hypoxia stress induced by submergence,a condition often arising from overirrigation,ponding,rainstorms,or flooding.While b...The rapid elongation of rice(Oryza sativa)coleoptile is pivotal for the plant plumule to evade hypoxia stress induced by submergence,a condition often arising from overirrigation,ponding,rainstorms,or flooding.While brassinosteroids(BRs)are recognized for their diverse roles in plant growth and development,their influence on coleoptile elongation under hypoxic conditions remains largely unexplored.In this study,we demonstrate the significant requirement of BRs for coleoptile elongation in deep water.During coleoptile development,Glycogen Synthase Kinase3-Like Kinase2(GSK2),the central inhibitor of BR signaling in rice,undergoes substantial suppression in deep water but induction in air.In contrast,the dephosphorylated form of BRASSINAZOLE RESISTANT1(OsBZR1),representing the active form of the key BR signaling transcription factor,is induced in water but suppressed in air.Remarkably,the knockout of GSK3-like kinase genes significantly enhances coleoptile elongation in deep water,strongly indicating a vital contribution of BR response to hypoxia-stimulated coleoptile elongation.Transcriptome analysis uncovers both BR-associated and BR-independent hypoxia responses,implicating substance metabolism,redox reactions,abiotic stress responses,and crosstalk with other hormones in the regulation of BR-induced hypoxia responses.In summary,our findings suggest that rice plumules rapidly elongate coleoptiles through the activation of BR response in deep water,enabling them to escape from submergence-induced hypoxia stress.展开更多
Nano-biochar(nano-BC)is one of the most active fractions in the BC continuum and frequently detected in terrestrial ecosystems.However,a paucity of information exists on reactivity and environmental functions of nano-...Nano-biochar(nano-BC)is one of the most active fractions in the BC continuum and frequently detected in terrestrial ecosystems.However,a paucity of information exists on reactivity and environmental functions of nano-BC in the rhizosphere.The present study investigated the potential of nano-BC in transforming silver ions(Ag^(+))to silver nanoparticles(AgNPs)in the rhizosphere of rice.We found that the synergistic effect of nano-BC and dioxygen secreted from rice roots was essential for Ag^(+)reduction to AgNPs.In this process,nano-BC transferred electrons to dioxygen,resulting in the formation of superoxide free radicals,which subsequently donate electrons to Ag^(+).Notably,excess nano-BC was unfavorable to dioxygen secretion from roots and thus inhibited the formation of AgNPs.Our results highlight that although nano-BC significantly decreased the uptake of Ag by rice plants,it contributed to the accumulation of AgNPs in plant tissues.TEM and single-particle ICP-MS analyses confirmed the presence of AgNPs not only in intercellular spaces of leaf tissues but also within the interior of leaf cells.These findings indicate that nano-BC plays a critical role in regulating the chemical species and bioaccumulation of redox-active metals(such as Ag)in the rhizosphere,which has important implications for element cycling from the pedosphere to terrestrial vegetation and warrants further investigation.展开更多
Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic function.Here,an anatomically correct IVD scaf...Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic function.Here,an anatomically correct IVD scaffold composed of biomaterials,cells,and growth factors were fabricated via three-dimensional(3D)bioprinting technology.Connective tissue growth factor(CTGF)and transforming growth factor-β3(TGF-β3)were loaded onto polydopamine nanoparticles,which were mixed with bone marrow mesenchymal stem cells(BMSCs)for regenerating and simulating the structure and function of the nucleus pulposus and annular fibrosus.In vitro experiments confirmed that CTGF and TGF-β3 could be released from the IVD scaffold in a spatially controlled manner,and induced the corresponding BMSCs to differentiate into nucleus pulposus like cells and annulus fibrosus like cells.Next,the fabricated IVD scaffold was implanted into the dorsum subcutaneous of nude mice.The reconstructed IVD exhibited a zone-specific matrix that displayed the corresponding histological and immunological phenotypes:primarily type II collagen and glycosaminoglycan in the core zone,and type I collagen in the surrounding zone.The testing results demonstrated that it exhibited good biomechanical function of the reconstructed IVD.The results presented herein reveal the clinical application potential of the dual growth factors-releasing IVD scaffold fabricated via 3D bioprinting.However,the evaluation in large mammal animal models needs to be further studied.展开更多
Articular cartilage injury is a common disease in the field of orthopedics.Because cartilage has poor self-repairing ability,medical intervention is needed.Using melt electro-writing(MEW)technology,tissue engineering ...Articular cartilage injury is a common disease in the field of orthopedics.Because cartilage has poor self-repairing ability,medical intervention is needed.Using melt electro-writing(MEW)technology,tissue engineering scaffolds with high porosity and high precision can be prepared.However,ordinary materials,especially natural polymer materials,are difficult to print.In this study,gelatin was mixed with poly(lactic-co-glycolic acid)to prepare high-concentration and high-viscosity printer ink,which had good printability and formability.A composite scaffold with full-layer TGF-β1 loading mixed with hydroxyapatite was prepared,and the scaffold was implanted at the cartilage injury site;microfracture surgery was conducted to induce the mesenchyme in the bone marrow.Quality stem cells thereby promoted the repair of damaged cartilage.In summary,this study developed a novel printing method,explored the molding conditions based on MEW printing ink,and constructed a bioactive cartilage repair scaffold.The scaffold can use autologous bone marrow mesenchymal stem cells and induce their differentiation to promote cartilage repair.展开更多
Tissue engineering focuses on repairing tissue and restoring tissue functions by employing three elements： scaffolds, cells and biochemical signals. In tissue engineering, bioactive material scaffolds have been used ...Tissue engineering focuses on repairing tissue and restoring tissue functions by employing three elements： scaffolds, cells and biochemical signals. In tissue engineering, bioactive material scaffolds have been used to cure tissue and organ defects with stem cell-based therapies being one of the best documented approaches. In the review, different biomaterials which are used in several methods to fabricate tissue engineering scaffolds were explained and show good properties （biocompatibility, biodegradability, and mechanical properties etc.） for cell migration and infiltration. Stem cell homing is a recruitment process for inducing the migration of the systemically transplanted cells, or host cells, to defect sites. The mechanisms and modes of stem cell homing-based tissue engineering can be divided into two types depending on the source of the stem cells： endogenous and exogenous. Exogenous stem cell-based bioactive scaffolds have the challenge of long-term culturing in vitro and for endogenous stem cells the biochemical signal homing recruitment mechanism is not clear yet. Although the stem cell homing-based bioactive scaffolds are attractive candidates for tissue defect therapies, based on in vitro studies and animal tests, there is still a long way before clinical application.展开更多
The conjunctiva is crucial in safeguarding the eye from harm or infection,thereby ensuring the preservation of the vision.The repair of infected conjunctival damage is necessary.The objective of this study is to devel...The conjunctiva is crucial in safeguarding the eye from harm or infection,thereby ensuring the preservation of the vision.The repair of infected conjunctival damage is necessary.The objective of this study is to develop copper-doped flexible silica nanofibers(SiO_(2)@Cu NFs)with multifunctional antibacterial and anti-inflammatory characteristics.The continuous release of copper ions from electrospun membranes is shown to be effective to promote antibacterial and bioactive functions.Nanofiber membranes also exhibit biocompatibility and promote cell growth,angiogenesis,and inflammation modulation.In vivo evaluations further reveal the therapeutic efficacy of SiO_(2)@Cu NFs to promote the structural and the functional recoveries of the conjunctiva.Taken together,SiO_(2)@Cu NFs may hold significant promise for the fabrication of alterna-tive ocular bandage to suppress bacterial infection and promote repair of ocular tissues and may potential be also used for related disciplines.展开更多
Marrow niches in osteosarcoma(OS)are a specialized microenvironment that is essential for the maintenance and regulation of OS cells.However,existing animal xenograft models are plagued by variability,complexity,and h...Marrow niches in osteosarcoma(OS)are a specialized microenvironment that is essential for the maintenance and regulation of OS cells.However,existing animal xenograft models are plagued by variability,complexity,and high cost.Herein,we used a decellularized osteosarcoma extracellular matrix(dOsEM)loaded with extracellular vesicles from human bone marrow-derived stem cells(hBMSC-EVs)and OS cells as a bioink to construct a micro-osteosarcoma(micro-OS)through 3D printing.The micro-OS was further combined with a microfluidic system to develop into an OS-on-a-chip(OOC)with a built-in recirculating perfusion system.The OOC system successfully integrated bone marrow niches,cell‒cell and cell-matrix crosstalk,and circulation,allowing a more accurate representation of OS characteristics in vivo.Moreover,the OOC system may serve as a valuable research platform for studying OS biological mechanisms compared with traditional xenograft models and is expected to enable precise and rapid evaluation and consequently more effective and comprehensive treatments for OS.展开更多
Despite recent progress in multiple myeloma(MM)treatments,most patients will relapse and require additional treatment.Intravenous daratumumab,a human IgGκmonoclonal antibody targeting CD38,has shown good efficacy in ...Despite recent progress in multiple myeloma(MM)treatments,most patients will relapse and require additional treatment.Intravenous daratumumab,a human IgGκmonoclonal antibody targeting CD38,has shown good efficacy in the treatment of MM.A subcutaneous version of daratumumab was formulated to reduce the burden of intravenous infusions.We aimed to investigate the efficacy and safety of subcutaneous daratumumab in Chinese patients with relapsed/refractory MM based on the demonstrated noninferiority of subcutaneous daratumumab to intravenous daratumumab,with a shorter administration time and reduced infusion-related reaction rate in global studies.This phase 1,multicenter study(MMY1010;ClinicalTrials.gov Identifier:NCT04121260)evaluated subcutaneous daratumumab in Chinese patients with relapsed/refractory MM after 1 prior line(n=1)or≥2 prior lines(n=20)of therapy,including a proteasome inhibitor and an immunomodulatory drug.Primary endpoints were pharmacokinetics and safety.Mean(standard deviation)maximum trough concentration of daratumumab was 826(335)μg/mL,which was consistent with prior studies of subcutaneous daratumumab and intravenous daratumumab.Safety was consistent with safety profiles observed in other daratumumab studies,with no new safety concerns identified.Incidences of infusion-related reactions and injection-site reactions were low and consistent with other subcutaneous daratumumab studies.At a median follow-up of 7.5 months,the overall response rate was 57.1%,with a very good partial response or better rate of 38.1%and complete response or better rate of 19.0%.Our results demonstrate a favorable benefit/risk profile of subcutaneous daratumumab in Chinese patients with relapsed/refractory MM,potentially impacting clinical administration of daratumumab in this population.展开更多
Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P（LLA-CL） nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the mat...Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P（LLA-CL） nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the material as tissue engineering nerve scaffold was investigated in vitro. The average diameter increased with decreasing the blend ratio of ApF to P（LLA-CL）. Characterization of 13C NMR and FTIR clarified that there is no obvious chemical bond reaction between ApF and P（LLA-CL）. The tensile strength and elongation at break increased with the content increase of P（LLA-CL）. The surface hydrophilic property of nanofibrous scaffolds enhanced with the increased content of ApF. Cell viability studies with Schwann cells demonstrated that ApFIP（LLA-CL） blended nanofibrous scaffolds significantly promoted cell growth as compare to P（LLA-CL）, especially when the weight ratio of ApF to P（LLA-CL） was 25：75. The present work provides a basis for further studies of this novel nanofibrous material （ApF/P（LLA-CL）） in peripheral nerve tissue repair or regeneration.展开更多
Massive hemorrhage may be detrimental to the patients,which necessitates the advent of new materials with high hemostatic efficiency and good biocompatibility.The objective of this research was to screen for the effec...Massive hemorrhage may be detrimental to the patients,which necessitates the advent of new materials with high hemostatic efficiency and good biocompatibility.The objective of this research was to screen for the effect of the different types of bio-elastomers as hemostatic dressings.3D loose nanofiber sponges were prepared;PU-TA/Gel showed promising potential.Polyurethane(PU)was synthesized and electrospun to afford porous sponges,which were crosslinked with glutaraldehyde(GA).FTIR and 1H-NMR evidenced the successful synthesis of PU.The prepared PU-TA/Gel sponge had the highest porosity and water absorption ratio.Besides,PU-TA/Gel sponges exhibited cytocompatibility,negligible hemolysis and the shortest clotting time.PU-TA/Gel sponge rapidly induced stable blood clots with shorter hemostasis time and less bleeding volume in a liver injury model in rats.Intriguingly,PU-TA/Gel sponges also induced good skin regeneration in a full-thickness excisional defect model as revealed by the histological analysis.These results showed that the PU-TA/Gel-based sponges may offer an alternative platform for hemostasis and wound healing.展开更多
Background:Small-diameter vascular grafts have become the focus of attention in tissue engineering.Thrombosis and aneurysmal dilatation are the two major complications of the loss of vascular access after surgery.Ther...Background:Small-diameter vascular grafts have become the focus of attention in tissue engineering.Thrombosis and aneurysmal dilatation are the two major complications of the loss of vascular access after surgery.Therefore,we focused on fabricating 3D printed electrospun vascular grafts loaded with tetramethylpyrazine(TMP)to overcome these limitations.Methods:Based on electrospinning and 3D printing,3D-printed electrospun vascular grafts loaded with TMP were fabricated.The inner layer of the graft was composed of electrospun poly(L-lactic-cocaprolactone)(PLCL)nanofibers and the outer layer consisted of 3D printed polycaprolactone(PCL)microfibers.The characterization and mechanical properties were tested.The blood compatibility and in vitro cytocompatibility of the grafts were also evaluated.Additionally,rat abdominal aortas were replaced with these 3D-printed electrospun grafts to evaluate their biosafety.Results:Mechanical tests demonstrated that the addition of PCL microfibers could improve the mechanical properties.In vitro experimental data proved that the introduction of TMP effectively inhibited platelet adhesion.Afterwards,rat abdominal aorta was replaced with 3D-printed electrospun grafts.The 3D-printed electrospun graft loaded with TMP showed good biocompatibility and mechanical strength within 6 months and maintained substantial patency without the occurrence of acute thrombosis.Moreover,no obvious aneurysmal dilatation was observed.Conclusions:The study demonstrated that 3D-printed electrospun vascular grafts loaded with TMP may have the potential for injured vascular healing.展开更多
Anti-scaling technology for pipelines has always been a focus of oilfield industrial production.Compared with traditional metal pipes,polyethylene(PE)pipes have unique advantages in terms of corrosion resistance,surfa...Anti-scaling technology for pipelines has always been a focus of oilfield industrial production.Compared with traditional metal pipes,polyethylene(PE)pipes have unique advantages in terms of corrosion resistance,surface friction resistance,and service life.In this paper,aiming at an enhancement of antiscaling and corrosion-resistant properties,as well as increased mechanical properties,PE nanocomposites have been prepared by the introduction of modified carbon nanotubes(m-CNTs)into the PE matrix.To improve the interface compatibility of the composites,the CNTs were treated with reactive tetrabutyl titanate after nitric acid oxidation,which brings about uniform dispersion of the CNTs and intimate interface interaction.As the m-CNT fraction increases,the PE crystallinity displays a slight increase.Polarized microscopy shows that the scaling on the surface of the composite material is obviously reduced compared with pure PE,because the surface free energy of the composite material decreases.Moreover,due to the good dispersion,the composites show enhanced mechanical properties.That is,by adding 1.10 wt%CNTs,the tensile stress and impact toughness of the composites are 20.76 MPa and 37.89 kJ m^(-2),respectively,increases of 15.0% and 11.9% compared with pure PE.This paper supports the idea that the crystallinity of the PE matrix can be improved by adding CNTs,thereby increasing the corrosion resistance and anti-scaling properties.This work can provide inspiration for using the methods of scale inhibition and corrosion resistance in polymer nanocomposites.展开更多
基金supported by the Medical Science Research Project of Hebei(No.20241512).
文摘Objectives This study aimed to examine the associations between depression,resilience,and fatigue in patients with multivessel coronary disease and verify their causal relationships.Methods Between October 2023 and June 2024,316 patients with multivessel coronary disease were recruited from three tertiary hospitals in Tangshan,China.The Patient Health Questionnaire,Connor-Davidson Resilience Scale,and the Multidimensional Fatigue Inventory were administered to the patients on the third day of admission(T1),one month after discharge(T2),and three months after discharge(T3).Pearson correlation analysis was conducted to examine the relationships among depression,resilience,and fatigue in patients with multivessel coronary disease,and cross-lagged analysis to explore the temporal causal relationships.Results In patients with multivessel coronary disease,levels of depression and fatigue decreased from T1 to T3,while resilience scores increased during the same period.The correlation analysis revealed significant relationships among depression,resilience,and fatigue at T1,T2,and T3(P<0.01).The autoregressive paths indicated high stability over time for depression,medium stability for resilience,and low stability for fatigue.Cross-lagged paths demonstrated that depression at T1 significantly predicted fatigue at T2(β=0.461,P<0.001),and depression at T2 significantly predicted fatigue at T3(β=0.957,P<0.001).And resilience at T1 significantly predicted fatigue at T2(β=−0.271,P<0.001),and resilience at T2 significantly predicted fatigue at T3(β=−0.176,P<0.001).Additionally,resilience had a moderating effect on the relationship between depression and fatigue(β=−0.760,P<0.001).Conclusions Our study confirmed that depression and resilience predicted fatigue in patients with multivessel coronary disease.To prevent and mitigate fatigue,alleviating depressive symptoms and enhancing resilience levels in patients at an early stage is essential.
基金supported by the National Natural Science Foundation of China ( 81100897 and 81100926 )the Natural Science Foundation of Chongqing Municipality, China (cstc2011jj A0856)
文摘Increasing evidence suggests that white matter disorders based on myelin sheath impairment may underlie the neuropathological changes in schizophrenia.But it is unknown whether enhancing remyelination is a beneficial approach to schizophrenia.To investigate this hypothesis,we used clemastine,an FDA-approved drug with high potency in promoting oligodendroglial differentiation and myelination,on a cuprizone-induced mouse model of demyelination.The mice exposed to cuprizone（0.2%in chow） for 6 weeks displayed schizophrenia-like behavioral changes,including decreased exploration of the center in the open field test and increased entries into the arms of the Y-maze,as well as evident demyelination in the cortex and corpus callosum.Clemastine treatment was initiated upon cuprizone withdrawal at 10 mg/kg per day for3 weeks.As expected,myelin repair was greatly enhanced in the demyelinated regions with increased mature oligodendrocytes（APC-positive） and myelin basic protein.More importantly,the clemastine treatment rescued the schizophrenia-like behavioral changes in the open field test and the Y-maze compared to vehicle,suggesting a beneficial effect via promoting myelin repair.Our findings indicate that enhancing remyelination may be a potential therapy for schizophrenia.
基金supported by STI 2030–Major Projects (2023ZD0407101)National Key Research and Development Program of China (2022YFD1201700)+1 种基金National Natural Science Foundation (U21A20208,32201704)Innovation Program of CAAS。
文摘The rapid elongation of rice(Oryza sativa)coleoptile is pivotal for the plant plumule to evade hypoxia stress induced by submergence,a condition often arising from overirrigation,ponding,rainstorms,or flooding.While brassinosteroids(BRs)are recognized for their diverse roles in plant growth and development,their influence on coleoptile elongation under hypoxic conditions remains largely unexplored.In this study,we demonstrate the significant requirement of BRs for coleoptile elongation in deep water.During coleoptile development,Glycogen Synthase Kinase3-Like Kinase2(GSK2),the central inhibitor of BR signaling in rice,undergoes substantial suppression in deep water but induction in air.In contrast,the dephosphorylated form of BRASSINAZOLE RESISTANT1(OsBZR1),representing the active form of the key BR signaling transcription factor,is induced in water but suppressed in air.Remarkably,the knockout of GSK3-like kinase genes significantly enhances coleoptile elongation in deep water,strongly indicating a vital contribution of BR response to hypoxia-stimulated coleoptile elongation.Transcriptome analysis uncovers both BR-associated and BR-independent hypoxia responses,implicating substance metabolism,redox reactions,abiotic stress responses,and crosstalk with other hormones in the regulation of BR-induced hypoxia responses.In summary,our findings suggest that rice plumules rapidly elongate coleoptiles through the activation of BR response in deep water,enabling them to escape from submergence-induced hypoxia stress.
基金Funding Project for Young Backbone Teachers to Visit and Study in China(JNFX2023061)Chuzhou University Research Initiation Fund Project(2023qd49,2023qd44)+4 种基金National Natural Science Foundation of China(42207435,42477263)Natural Science Foundation of Hebei Province(B2023202077,D2022202004)Natural Science Foundation of Tianjin(23JCYBJC00940)China Postdoctoral Science Foundation(2020M680868)Young Scientists Fund-National Science Foundation of China(42207048).
文摘Nano-biochar(nano-BC)is one of the most active fractions in the BC continuum and frequently detected in terrestrial ecosystems.However,a paucity of information exists on reactivity and environmental functions of nano-BC in the rhizosphere.The present study investigated the potential of nano-BC in transforming silver ions(Ag^(+))to silver nanoparticles(AgNPs)in the rhizosphere of rice.We found that the synergistic effect of nano-BC and dioxygen secreted from rice roots was essential for Ag^(+)reduction to AgNPs.In this process,nano-BC transferred electrons to dioxygen,resulting in the formation of superoxide free radicals,which subsequently donate electrons to Ag^(+).Notably,excess nano-BC was unfavorable to dioxygen secretion from roots and thus inhibited the formation of AgNPs.Our results highlight that although nano-BC significantly decreased the uptake of Ag by rice plants,it contributed to the accumulation of AgNPs in plant tissues.TEM and single-particle ICP-MS analyses confirmed the presence of AgNPs not only in intercellular spaces of leaf tissues but also within the interior of leaf cells.These findings indicate that nano-BC plays a critical role in regulating the chemical species and bioaccumulation of redox-active metals(such as Ag)in the rhizosphere,which has important implications for element cycling from the pedosphere to terrestrial vegetation and warrants further investigation.
基金supported by National Key R&D Program of China(No.2018YFB1105600,No.2018YFA0703000)National Natural Science Foundation of China(No.81802131)Project funded by China Postdoctoral Science Foundation(No.2019T120347)and the fund of No.XK2019013.
文摘Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic function.Here,an anatomically correct IVD scaffold composed of biomaterials,cells,and growth factors were fabricated via three-dimensional(3D)bioprinting technology.Connective tissue growth factor(CTGF)and transforming growth factor-β3(TGF-β3)were loaded onto polydopamine nanoparticles,which were mixed with bone marrow mesenchymal stem cells(BMSCs)for regenerating and simulating the structure and function of the nucleus pulposus and annular fibrosus.In vitro experiments confirmed that CTGF and TGF-β3 could be released from the IVD scaffold in a spatially controlled manner,and induced the corresponding BMSCs to differentiate into nucleus pulposus like cells and annulus fibrosus like cells.Next,the fabricated IVD scaffold was implanted into the dorsum subcutaneous of nude mice.The reconstructed IVD exhibited a zone-specific matrix that displayed the corresponding histological and immunological phenotypes:primarily type II collagen and glycosaminoglycan in the core zone,and type I collagen in the surrounding zone.The testing results demonstrated that it exhibited good biomechanical function of the reconstructed IVD.The results presented herein reveal the clinical application potential of the dual growth factors-releasing IVD scaffold fabricated via 3D bioprinting.However,the evaluation in large mammal animal models needs to be further studied.
基金This work was supported by Shanghai Ninth People’s Hospital(grant number XK2019013)National Natural Science Foundation of China(No.81802131,82002293)China Postdoctoral Science Foundation(No.2019T120347).
文摘Articular cartilage injury is a common disease in the field of orthopedics.Because cartilage has poor self-repairing ability,medical intervention is needed.Using melt electro-writing(MEW)technology,tissue engineering scaffolds with high porosity and high precision can be prepared.However,ordinary materials,especially natural polymer materials,are difficult to print.In this study,gelatin was mixed with poly(lactic-co-glycolic acid)to prepare high-concentration and high-viscosity printer ink,which had good printability and formability.A composite scaffold with full-layer TGF-β1 loading mixed with hydroxyapatite was prepared,and the scaffold was implanted at the cartilage injury site;microfracture surgery was conducted to induce the mesenchyme in the bone marrow.Quality stem cells thereby promoted the repair of damaged cartilage.In summary,this study developed a novel printing method,explored the molding conditions based on MEW printing ink,and constructed a bioactive cartilage repair scaffold.The scaffold can use autologous bone marrow mesenchymal stem cells and induce their differentiation to promote cartilage repair.
文摘Tissue engineering focuses on repairing tissue and restoring tissue functions by employing three elements： scaffolds, cells and biochemical signals. In tissue engineering, bioactive material scaffolds have been used to cure tissue and organ defects with stem cell-based therapies being one of the best documented approaches. In the review, different biomaterials which are used in several methods to fabricate tissue engineering scaffolds were explained and show good properties （biocompatibility, biodegradability, and mechanical properties etc.） for cell migration and infiltration. Stem cell homing is a recruitment process for inducing the migration of the systemically transplanted cells, or host cells, to defect sites. The mechanisms and modes of stem cell homing-based tissue engineering can be divided into two types depending on the source of the stem cells： endogenous and exogenous. Exogenous stem cell-based bioactive scaffolds have the challenge of long-term culturing in vitro and for endogenous stem cells the biochemical signal homing recruitment mechanism is not clear yet. Although the stem cell homing-based bioactive scaffolds are attractive candidates for tissue defect therapies, based on in vitro studies and animal tests, there is still a long way before clinical application.
基金supported by Science and Technology Commission of Shanghai Municipality,China(Nos.20S31900900,20DZ2254900)Sino German Science Foundation Research Exchange Center,China(M-0263)+7 种基金China Education Association for International Exchange(2022181)supported by Researchers Supporting Project Number(RSP2024R65)King Saud University,Riyadh,Saudi Arabia.Moreover,this project was supported by the State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,Donghua University(KF2109)National Natural Science Foundation of China(No.82070919 and 82271041)the Program of Shanghai Academic/Technology Research Leader(22XD1401800)the Biomaterials and Regenerative Medicine Institute Cooperative Research Project,Shanghai Jiao Tong University School of Medicine(2022LHA06)Shanghai Key Clinical Specialty,and Shanghai Eye Disease Research Center(2022ZZ01003)supported by the Fundamental Research Funds for the Central Universities(CUSF-DH-T-2023064).
文摘The conjunctiva is crucial in safeguarding the eye from harm or infection,thereby ensuring the preservation of the vision.The repair of infected conjunctival damage is necessary.The objective of this study is to develop copper-doped flexible silica nanofibers(SiO_(2)@Cu NFs)with multifunctional antibacterial and anti-inflammatory characteristics.The continuous release of copper ions from electrospun membranes is shown to be effective to promote antibacterial and bioactive functions.Nanofiber membranes also exhibit biocompatibility and promote cell growth,angiogenesis,and inflammation modulation.In vivo evaluations further reveal the therapeutic efficacy of SiO_(2)@Cu NFs to promote the structural and the functional recoveries of the conjunctiva.Taken together,SiO_(2)@Cu NFs may hold significant promise for the fabrication of alterna-tive ocular bandage to suppress bacterial infection and promote repair of ocular tissues and may potential be also used for related disciplines.
基金the Shanghai Pujiang Program(21PJ1409200)the China Postdoctoral Science Foundation(2022M722122)+6 种基金Three-year Action Plan of Shenkang Development Center(SHDC2020CR2019B)Biomaterials and Regenerative Medicine Institute Cooperative Research Project,Shanghai Jiao Tong University School of Medicine(2022LHB07,2022LHA01)Shanghai Frontiers Science Center of Degeneration and Regeneration in Skeletal Systemthe Fundamental Research Funds for the Central Universities(YG2023LC07)Clinical Research Program of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYLJ202122)the Project of Biobank from Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(YBKB202116)the National Scientific Foundation of China(82171993,81972058,82130073).
文摘Marrow niches in osteosarcoma(OS)are a specialized microenvironment that is essential for the maintenance and regulation of OS cells.However,existing animal xenograft models are plagued by variability,complexity,and high cost.Herein,we used a decellularized osteosarcoma extracellular matrix(dOsEM)loaded with extracellular vesicles from human bone marrow-derived stem cells(hBMSC-EVs)and OS cells as a bioink to construct a micro-osteosarcoma(micro-OS)through 3D printing.The micro-OS was further combined with a microfluidic system to develop into an OS-on-a-chip(OOC)with a built-in recirculating perfusion system.The OOC system successfully integrated bone marrow niches,cell‒cell and cell-matrix crosstalk,and circulation,allowing a more accurate representation of OS characteristics in vivo.Moreover,the OOC system may serve as a valuable research platform for studying OS biological mechanisms compared with traditional xenograft models and is expected to enable precise and rapid evaluation and consequently more effective and comprehensive treatments for OS.
文摘Despite recent progress in multiple myeloma(MM)treatments,most patients will relapse and require additional treatment.Intravenous daratumumab,a human IgGκmonoclonal antibody targeting CD38,has shown good efficacy in the treatment of MM.A subcutaneous version of daratumumab was formulated to reduce the burden of intravenous infusions.We aimed to investigate the efficacy and safety of subcutaneous daratumumab in Chinese patients with relapsed/refractory MM based on the demonstrated noninferiority of subcutaneous daratumumab to intravenous daratumumab,with a shorter administration time and reduced infusion-related reaction rate in global studies.This phase 1,multicenter study(MMY1010;ClinicalTrials.gov Identifier:NCT04121260)evaluated subcutaneous daratumumab in Chinese patients with relapsed/refractory MM after 1 prior line(n=1)or≥2 prior lines(n=20)of therapy,including a proteasome inhibitor and an immunomodulatory drug.Primary endpoints were pharmacokinetics and safety.Mean(standard deviation)maximum trough concentration of daratumumab was 826(335)μg/mL,which was consistent with prior studies of subcutaneous daratumumab and intravenous daratumumab.Safety was consistent with safety profiles observed in other daratumumab studies,with no new safety concerns identified.Incidences of infusion-related reactions and injection-site reactions were low and consistent with other subcutaneous daratumumab studies.At a median follow-up of 7.5 months,the overall response rate was 57.1%,with a very good partial response or better rate of 38.1%and complete response or better rate of 19.0%.Our results demonstrate a favorable benefit/risk profile of subcutaneous daratumumab in Chinese patients with relapsed/refractory MM,potentially impacting clinical administration of daratumumab in this population.
基金This research was supported by the National Key Research Program of China （2016YFA0201702 of 2016YFA0201700）, the National Natural Science Foundation of China （Grant Nos. 31470941 and 31271035）, the Science and Technology Commission of Shanghai Municipality （Grant Nos. 15JC1490100 and 15441905100）, the Ph.D. Programs Foundation of Ministry of Education of China （Grant No. 20130075110005）, and the Yantai Double Hundred Talent Plan. The authors extend their appreciation to the International Scientific Partnership Program 1SPP at King Saud University for funding this research work through ISPP# 0049.
文摘Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P（LLA-CL） nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the material as tissue engineering nerve scaffold was investigated in vitro. The average diameter increased with decreasing the blend ratio of ApF to P（LLA-CL）. Characterization of 13C NMR and FTIR clarified that there is no obvious chemical bond reaction between ApF and P（LLA-CL）. The tensile strength and elongation at break increased with the content increase of P（LLA-CL）. The surface hydrophilic property of nanofibrous scaffolds enhanced with the increased content of ApF. Cell viability studies with Schwann cells demonstrated that ApFIP（LLA-CL） blended nanofibrous scaffolds significantly promoted cell growth as compare to P（LLA-CL）, especially when the weight ratio of ApF to P（LLA-CL） was 25：75. The present work provides a basis for further studies of this novel nanofibrous material （ApF/P（LLA-CL）） in peripheral nerve tissue repair or regeneration.
基金supported by the Science and Technology Commission of Shanghai Municipality(19441902600,20S31900900,20DZ2254900)Sino German Science Foundation Research Exchange Center(M-0263)+3 种基金the crossdisciplinary project of Donghua University(101080241022)This project was also supported by Researchers Supporting Project Number(RSP2023R65)King Saud University,Riyadh,Saudi Arabia.M.S.is an International Research Fellow of the Japan Society for the Promotion of Science(Postdoctoral Fellowships for Research in Japan(Standard))The part of this research was also funded by Grant-in-Aid for JSPS Fellows(JP21F21353).
文摘Massive hemorrhage may be detrimental to the patients,which necessitates the advent of new materials with high hemostatic efficiency and good biocompatibility.The objective of this research was to screen for the effect of the different types of bio-elastomers as hemostatic dressings.3D loose nanofiber sponges were prepared;PU-TA/Gel showed promising potential.Polyurethane(PU)was synthesized and electrospun to afford porous sponges,which were crosslinked with glutaraldehyde(GA).FTIR and 1H-NMR evidenced the successful synthesis of PU.The prepared PU-TA/Gel sponge had the highest porosity and water absorption ratio.Besides,PU-TA/Gel sponges exhibited cytocompatibility,negligible hemolysis and the shortest clotting time.PU-TA/Gel sponge rapidly induced stable blood clots with shorter hemostasis time and less bleeding volume in a liver injury model in rats.Intriguingly,PU-TA/Gel sponges also induced good skin regeneration in a full-thickness excisional defect model as revealed by the histological analysis.These results showed that the PU-TA/Gel-based sponges may offer an alternative platform for hemostasis and wound healing.
基金supported by the Science and Technology Commission of Shanghai Municipality,China(Nos.20S31900900,20DZ2254900)the Sino German Science Foundation Research Exchange Center,China(M-0263)+3 种基金China Education Association for International Exchange(2022181)supported by the General Project of SHDC(SHDC22021213)Fundamental Research Funds for the Central Universities(No.2232023D-10).This project was also supported by Researchers Supporting Project Number(RSP2024R65)King Saud University,Riyadh,Saudi Arabia.
文摘Background:Small-diameter vascular grafts have become the focus of attention in tissue engineering.Thrombosis and aneurysmal dilatation are the two major complications of the loss of vascular access after surgery.Therefore,we focused on fabricating 3D printed electrospun vascular grafts loaded with tetramethylpyrazine(TMP)to overcome these limitations.Methods:Based on electrospinning and 3D printing,3D-printed electrospun vascular grafts loaded with TMP were fabricated.The inner layer of the graft was composed of electrospun poly(L-lactic-cocaprolactone)(PLCL)nanofibers and the outer layer consisted of 3D printed polycaprolactone(PCL)microfibers.The characterization and mechanical properties were tested.The blood compatibility and in vitro cytocompatibility of the grafts were also evaluated.Additionally,rat abdominal aortas were replaced with these 3D-printed electrospun grafts to evaluate their biosafety.Results:Mechanical tests demonstrated that the addition of PCL microfibers could improve the mechanical properties.In vitro experimental data proved that the introduction of TMP effectively inhibited platelet adhesion.Afterwards,rat abdominal aorta was replaced with 3D-printed electrospun grafts.The 3D-printed electrospun graft loaded with TMP showed good biocompatibility and mechanical strength within 6 months and maintained substantial patency without the occurrence of acute thrombosis.Moreover,no obvious aneurysmal dilatation was observed.Conclusions:The study demonstrated that 3D-printed electrospun vascular grafts loaded with TMP may have the potential for injured vascular healing.
基金This work was supported by the research fund of China National Petroleum Corporation(No.2020E-2801(GF)).
文摘Anti-scaling technology for pipelines has always been a focus of oilfield industrial production.Compared with traditional metal pipes,polyethylene(PE)pipes have unique advantages in terms of corrosion resistance,surface friction resistance,and service life.In this paper,aiming at an enhancement of antiscaling and corrosion-resistant properties,as well as increased mechanical properties,PE nanocomposites have been prepared by the introduction of modified carbon nanotubes(m-CNTs)into the PE matrix.To improve the interface compatibility of the composites,the CNTs were treated with reactive tetrabutyl titanate after nitric acid oxidation,which brings about uniform dispersion of the CNTs and intimate interface interaction.As the m-CNT fraction increases,the PE crystallinity displays a slight increase.Polarized microscopy shows that the scaling on the surface of the composite material is obviously reduced compared with pure PE,because the surface free energy of the composite material decreases.Moreover,due to the good dispersion,the composites show enhanced mechanical properties.That is,by adding 1.10 wt%CNTs,the tensile stress and impact toughness of the composites are 20.76 MPa and 37.89 kJ m^(-2),respectively,increases of 15.0% and 11.9% compared with pure PE.This paper supports the idea that the crystallinity of the PE matrix can be improved by adding CNTs,thereby increasing the corrosion resistance and anti-scaling properties.This work can provide inspiration for using the methods of scale inhibition and corrosion resistance in polymer nanocomposites.
