Diffusible signal factor(DSF)-family quorum sensing(QS)signals are widely utilized by many pathogenic bacteria to modulate various biological functions and virulence.Previous studies showed that cis-2-decenoic acid(ci...Diffusible signal factor(DSF)-family quorum sensing(QS)signals are widely utilized by many pathogenic bacteria to modulate various biological functions and virulence.Previous studies showed that cis-2-decenoic acid(cis-DA)is involved in the modulation of biofilm dispersion in Pseudomonas aeruginosa,but the regulatory mechanism is unclear.Here,we report that cis-DA regulates the physiology and virulence of P.aeruginosa through FadD1,a long-chain fatty acid-CoA ligase.cis-DA specifically binds to FadD1 and enhances the binding ability of FadD1 to the target gene promoter DNA regions.Further analysis showed that FadD1 is a global regulatory factor that controls the transcription of various target genes.Moreover,FadD1 showed catalytic activity on cis-2-dodecenoic acid(BDSF)of Burkholderia cenocepacia and enhanced the competitiveness of P.aeruginosa.Together,our work presents a new DSF-type QS signaling system in P.aeruginosa,which is highlighted by the signal receptor evolved from a canonical enzyme of fatty acid metabolism.展开更多
Purpose Colorectal cancer is a common malignant tumor worldwide.In China,the ratio of rectal cancer to coloncancer in terms of incidence is close to 1:1.Low rectal cancer accounts for more than half of all cases of re...Purpose Colorectal cancer is a common malignant tumor worldwide.In China,the ratio of rectal cancer to coloncancer in terms of incidence is close to 1:1.Low rectal cancer accounts for more than half of all cases of rectal cancer.In recent years,the proportion of rectal cancer has trended downward,however the incidence of rectal cancer inyounger adults is increasing.The CACA Guidelines for Holistic Integrative Management of Rectal Cancer were editedto help improve the diagnosis and comprehensive treatment in China.Methods This guideline has been prepared by consensuses reached by the CACA Committee of Colorectal CancerSociety,based on a careful review of the latest evidence including China’s studies,and referred to domestic and internationalrelative guidelines,also considered China’s specific national conditions and clinical practice.Results The CACA Guidelines for Holistic Integrative Management of Rectal Cancer include the epidemiology of rectalcancer,prevention and screening,diagnosis,treatment of nonmetastatic and metastatic rectal cancer,follow-up,and whole-course rehabilitation management.Conclusion Committee of Colorectal Cancer Society,Chinese Anti-Cancer Association,standardizes the diagnosisand treatment of rectal cancer in China through the formulation of the CACA Guidelines.展开更多
基金financially supported by the National Natural Science Foundation of China(32300033)the National Key Research and Development Program of China(2021YFA0717003)+1 种基金the Hainan Provincial Natural Science Foundation of China(325RC647)the Scientific Research Foundation of Hainan University(KYQD(ZR)-23006).
文摘Diffusible signal factor(DSF)-family quorum sensing(QS)signals are widely utilized by many pathogenic bacteria to modulate various biological functions and virulence.Previous studies showed that cis-2-decenoic acid(cis-DA)is involved in the modulation of biofilm dispersion in Pseudomonas aeruginosa,but the regulatory mechanism is unclear.Here,we report that cis-DA regulates the physiology and virulence of P.aeruginosa through FadD1,a long-chain fatty acid-CoA ligase.cis-DA specifically binds to FadD1 and enhances the binding ability of FadD1 to the target gene promoter DNA regions.Further analysis showed that FadD1 is a global regulatory factor that controls the transcription of various target genes.Moreover,FadD1 showed catalytic activity on cis-2-dodecenoic acid(BDSF)of Burkholderia cenocepacia and enhanced the competitiveness of P.aeruginosa.Together,our work presents a new DSF-type QS signaling system in P.aeruginosa,which is highlighted by the signal receptor evolved from a canonical enzyme of fatty acid metabolism.
文摘Purpose Colorectal cancer is a common malignant tumor worldwide.In China,the ratio of rectal cancer to coloncancer in terms of incidence is close to 1:1.Low rectal cancer accounts for more than half of all cases of rectal cancer.In recent years,the proportion of rectal cancer has trended downward,however the incidence of rectal cancer inyounger adults is increasing.The CACA Guidelines for Holistic Integrative Management of Rectal Cancer were editedto help improve the diagnosis and comprehensive treatment in China.Methods This guideline has been prepared by consensuses reached by the CACA Committee of Colorectal CancerSociety,based on a careful review of the latest evidence including China’s studies,and referred to domestic and internationalrelative guidelines,also considered China’s specific national conditions and clinical practice.Results The CACA Guidelines for Holistic Integrative Management of Rectal Cancer include the epidemiology of rectalcancer,prevention and screening,diagnosis,treatment of nonmetastatic and metastatic rectal cancer,follow-up,and whole-course rehabilitation management.Conclusion Committee of Colorectal Cancer Society,Chinese Anti-Cancer Association,standardizes the diagnosisand treatment of rectal cancer in China through the formulation of the CACA Guidelines.
