Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributi...Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality.However,the details of their biosynthesis and metabolism in tea plants are still unknown.Here,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature treatment.Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of eugenol.Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78A15.Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic acid.The expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of tea.Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress.The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.展开更多
Lightning-generated nitrogen oxides(LNO_(x))have a major influence on the atmosphere and global climate change.Therefore,it is of great importance to obtain a more accurate estimation of LNO_(x).The aim of this study ...Lightning-generated nitrogen oxides(LNO_(x))have a major influence on the atmosphere and global climate change.Therefore,it is of great importance to obtain a more accurate estimation of LNO_(x).The aim of this study is to provide a reference for the accurate estimation of the total LNO_(x) in the mainland of China based on cloud-to-ground lightning(CG)location data from 2014 to 2018.The energy of each CG flash was based on the number of return strokes per CG flash,the peak current of each return stroke,and the assumed CG breakdown voltage.The energy of intracloud lightning(IC)was based on the estimated frequencies of IC and the assumed energy of each IC flash.Combining the energy of lightning and the number of nitric oxide(NO)molecules produced by unit energy(ρno),the total LNO_(x) production in the mainland of China was determined.The LNO_(x) in the mainland of China estimated in this study is in the range(0.157-0.321)×10^(9) kg per year[Tg(N)yr-1],which is on the high end of other scholars’works.Negative cloud-to-ground lightning(NCG)flashes produce the most moles of NO_(x),while positive cloud-to-ground lightning(PCG)flashes produce the least total moles of NO_(x).The breakdown voltage of PCG is greater than that of IC or NCG,while the latter has a greater output of LNO_(x).展开更多
In this study, we examine how the quantum circuit of the Advanced Encryption Standard(AES) can be optimized from two aspects, i.e., number of qubits and T-depth. To reduce the number of qubits, we present three kinds ...In this study, we examine how the quantum circuit of the Advanced Encryption Standard(AES) can be optimized from two aspects, i.e., number of qubits and T-depth. To reduce the number of qubits, we present three kinds of improved quantum circuits of S-box for different phases in the AES. We found that the number of qubits in the round function can be decreased by introducing the circuit sending |a> to |S(a)>. As a result, compared with the previous quantum circuits where 400/640/768 qubits are required,our circuits of AES-128/-192/-256 only require 270/334/398 qubits. To reduce the T-depth, we propose a new circuit of AES's S-box with a T-depth of 4. Accordingly, the T-depth of our AES-128/-192/-256 quantum circuits become 80/80/84 instead of120/120/126 in a previous study.展开更多
The major features of different road sections were identified.Methods quantitatively determining the ecological impact of highway construction were discussed,and a demonstration was presented.The Dabao highway(from Da...The major features of different road sections were identified.Methods quantitatively determining the ecological impact of highway construction were discussed,and a demonstration was presented.The Dabao highway(from Dali to Baoshan)and the Sixiao highway(from Simao to Xiaomengyang)passing through the Longitudinal Range-Gorge Region were used as examples in this paper.For the normal road sections having no important species to protect,a method of ecosystem health assessment was introduced to calculate the overall range of the ecological impact of highway construction.For the road sections having plants or soils of interest,indexes were selected and the range of impact was determined using mathematical methods such as regression analysis and variance analysis.The range of impact on animal populations was also discussed in terms of the minimum living areas required by animal populations and the fragmentation caused by highway construction.The results indicate that the zones impacted by highway construction were composed of both regular and anomalous figures,the range of impacts for different landforms of the two sample highways were substantially different;highway construction had dramatic effects on roadside ecosystem health;the impact on soil factors of farmland was greater than 200 m,and the distance may exceed 1000 m when important large animals were considered.展开更多
基金the Science Fund for the National Natural Science Foundation of China(Grant Nos.31961133030,31870678)the National Key Research and Development Program of China(Grant No.2018YFD1000601)+1 种基金Distinguished Young Scientists of Anhui Province(Grant No.1908085J12)the Graduate Innovation Fund(Grant No.2020ysj-32).
文摘Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality.However,the details of their biosynthesis and metabolism in tea plants are still unknown.Here,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature treatment.Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of eugenol.Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78A15.Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic acid.The expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of tea.Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress.The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.
基金supported by the National Natural Science Foundation of China (Grant Nos. 91537209 and 91644224)
文摘Lightning-generated nitrogen oxides(LNO_(x))have a major influence on the atmosphere and global climate change.Therefore,it is of great importance to obtain a more accurate estimation of LNO_(x).The aim of this study is to provide a reference for the accurate estimation of the total LNO_(x) in the mainland of China based on cloud-to-ground lightning(CG)location data from 2014 to 2018.The energy of each CG flash was based on the number of return strokes per CG flash,the peak current of each return stroke,and the assumed CG breakdown voltage.The energy of intracloud lightning(IC)was based on the estimated frequencies of IC and the assumed energy of each IC flash.Combining the energy of lightning and the number of nitric oxide(NO)molecules produced by unit energy(ρno),the total LNO_(x) production in the mainland of China was determined.The LNO_(x) in the mainland of China estimated in this study is in the range(0.157-0.321)×10^(9) kg per year[Tg(N)yr-1],which is on the high end of other scholars’works.Negative cloud-to-ground lightning(NCG)flashes produce the most moles of NO_(x),while positive cloud-to-ground lightning(PCG)flashes produce the least total moles of NO_(x).The breakdown voltage of PCG is greater than that of IC or NCG,while the latter has a greater output of LNO_(x).
基金supported by the National Natural Science Foundation of China (Grant Nos. 61972048, and 61976024)Beijing Natural Science Foundation (Grant No. 4222031)。
文摘In this study, we examine how the quantum circuit of the Advanced Encryption Standard(AES) can be optimized from two aspects, i.e., number of qubits and T-depth. To reduce the number of qubits, we present three kinds of improved quantum circuits of S-box for different phases in the AES. We found that the number of qubits in the round function can be decreased by introducing the circuit sending |a> to |S(a)>. As a result, compared with the previous quantum circuits where 400/640/768 qubits are required,our circuits of AES-128/-192/-256 only require 270/334/398 qubits. To reduce the T-depth, we propose a new circuit of AES's S-box with a T-depth of 4. Accordingly, the T-depth of our AES-128/-192/-256 quantum circuits become 80/80/84 instead of120/120/126 in a previous study.
基金the National Natural Science Foundation of China(Grant No.40701173)the Public Benefit Project of Scientific Research on Environmental Protection(Grant No.200809072)。
文摘The major features of different road sections were identified.Methods quantitatively determining the ecological impact of highway construction were discussed,and a demonstration was presented.The Dabao highway(from Dali to Baoshan)and the Sixiao highway(from Simao to Xiaomengyang)passing through the Longitudinal Range-Gorge Region were used as examples in this paper.For the normal road sections having no important species to protect,a method of ecosystem health assessment was introduced to calculate the overall range of the ecological impact of highway construction.For the road sections having plants or soils of interest,indexes were selected and the range of impact was determined using mathematical methods such as regression analysis and variance analysis.The range of impact on animal populations was also discussed in terms of the minimum living areas required by animal populations and the fragmentation caused by highway construction.The results indicate that the zones impacted by highway construction were composed of both regular and anomalous figures,the range of impacts for different landforms of the two sample highways were substantially different;highway construction had dramatic effects on roadside ecosystem health;the impact on soil factors of farmland was greater than 200 m,and the distance may exceed 1000 m when important large animals were considered.
