OBJECTIVE： Prostate cancer（PCa） is a major health concern. Calliandra portoricensis（CP） is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative pro...OBJECTIVE： Prostate cancer（PCa） is a major health concern. Calliandra portoricensis（CP） is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative properties for PCa still need to be investigated. METHODS： Antioxidant activities of CP were determined by 1,1-diphenyl-2-picryhydrazyl（DPPH） and hydroxyl（OH-） radicals-scavenging methods. PC-3 and LNCa P（androgen-refractory and androgendependent PCa-derived cell lines） were cultured and treated with CP（10, 50 and 100 μg/m L）. Effects of CP on cells were determined by cytotoxicity assay（lactate dehydrogenase, LDH） and viability assay（sodium 3′-[1-（phenylaminocarbonyl）-3,4-tetrazolium]-bis（4-methoxy-6-nitro） benzene sulfonic acid hydrate, XTT）. DNA fragmentation was detected by cell death detection enzyme-linked immunosorbent assay plus kit. CP was tested as an inhibitor of angiogenesis using chicken chorioallantoic membrane（CAM） assay. RESULTS： CP showed significant scavenging of DPPH and OH- radicals. CP significantly（P〈0.05） inhibited lipid peroxidation in a dose-dependent manner. Precisely, CP（10, 50 and 100 μg/m L） inhibited PC-3 and LNCa P growth by 7%, 74% and 92%, and 27%, 73%, and 85% respectively at 48 h. CP had low toxicity in vitro at its half inhibitory concentration dose. Detection of cell death induced by CP at 50 μg/m L showed higher enrichment factors in LNCa P（7.38±0.95） than PC-3（3.48±0.55）. Also, treatment with CP（50 μg/m L） significantly reduced network of vessels in CAM, suggesting its antiangiogenic potential. CONCLUSION： Calliandra portoricensis elicited antioxidant, antiangiogenic and antiproliferative effects in PCa cells.展开更多
Epigenetic mechanisms play an important role in the development and persistence of cancer,and histone deacetylase(HDAC)inhibitors are promising anticancer drugs targeting epigenetic modes.Efficient anticancer drugs fo...Epigenetic mechanisms play an important role in the development and persistence of cancer,and histone deacetylase(HDAC)inhibitors are promising anticancer drugs targeting epigenetic modes.Efficient anticancer drugs for the treatment of castration-resistant prostate cancer(CRPC)are sought,and approved HDAC inhibitors have shown promising results on the one hand and severe drawbacks on the other hand.Hence,ways to break the drug resistance mechanisms of existing HDAC inhibitors as well as the design of new promising HDAC inhibitors which can overcome the disadvantages of the classic HDAC inhibitors are of great importance.In this work,HDAC inhibitors with the potential to become a mainstay for the treatment of CRPC in the future as well as suitable combination treatments of HDAC inhibitors with other anticancer drugs leading to considerable synergistic effects in treated CRPCs are discussed.展开更多
基金supported by a 6-month grant given to OA by the Bank-Anthony Charitable Trust Will (UK) which was disbursed by the College of Medicine, University of Ibadan, Nigeria, Charite University of Medicine, Berlin and Urologic Research Foundation Berlin, Germany
文摘OBJECTIVE： Prostate cancer（PCa） is a major health concern. Calliandra portoricensis（CP） is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative properties for PCa still need to be investigated. METHODS： Antioxidant activities of CP were determined by 1,1-diphenyl-2-picryhydrazyl（DPPH） and hydroxyl（OH-） radicals-scavenging methods. PC-3 and LNCa P（androgen-refractory and androgendependent PCa-derived cell lines） were cultured and treated with CP（10, 50 and 100 μg/m L）. Effects of CP on cells were determined by cytotoxicity assay（lactate dehydrogenase, LDH） and viability assay（sodium 3′-[1-（phenylaminocarbonyl）-3,4-tetrazolium]-bis（4-methoxy-6-nitro） benzene sulfonic acid hydrate, XTT）. DNA fragmentation was detected by cell death detection enzyme-linked immunosorbent assay plus kit. CP was tested as an inhibitor of angiogenesis using chicken chorioallantoic membrane（CAM） assay. RESULTS： CP showed significant scavenging of DPPH and OH- radicals. CP significantly（P〈0.05） inhibited lipid peroxidation in a dose-dependent manner. Precisely, CP（10, 50 and 100 μg/m L） inhibited PC-3 and LNCa P growth by 7%, 74% and 92%, and 27%, 73%, and 85% respectively at 48 h. CP had low toxicity in vitro at its half inhibitory concentration dose. Detection of cell death induced by CP at 50 μg/m L showed higher enrichment factors in LNCa P（7.38±0.95） than PC-3（3.48±0.55）. Also, treatment with CP（50 μg/m L） significantly reduced network of vessels in CAM, suggesting its antiangiogenic potential. CONCLUSION： Calliandra portoricensis elicited antioxidant, antiangiogenic and antiproliferative effects in PCa cells.
文摘Epigenetic mechanisms play an important role in the development and persistence of cancer,and histone deacetylase(HDAC)inhibitors are promising anticancer drugs targeting epigenetic modes.Efficient anticancer drugs for the treatment of castration-resistant prostate cancer(CRPC)are sought,and approved HDAC inhibitors have shown promising results on the one hand and severe drawbacks on the other hand.Hence,ways to break the drug resistance mechanisms of existing HDAC inhibitors as well as the design of new promising HDAC inhibitors which can overcome the disadvantages of the classic HDAC inhibitors are of great importance.In this work,HDAC inhibitors with the potential to become a mainstay for the treatment of CRPC in the future as well as suitable combination treatments of HDAC inhibitors with other anticancer drugs leading to considerable synergistic effects in treated CRPCs are discussed.
