Objective:Histology grade,subtypes and TNM stage of lung adenocarcinomas are useful predictors of prognosis and survival.The aim of the study was to investigate the relationship between chromosomal instability,morphol...Objective:Histology grade,subtypes and TNM stage of lung adenocarcinomas are useful predictors of prognosis and survival.The aim of the study was to investigate the relationship between chromosomal instability,morphological subtypes and the grading system used in lung non-mucinous adenocarcinoma(LNMA).Methods:We developed a whole genome copy number variation(WGCNV)scoring system and applied next generation sequencing to evaluate CNVs present in 91 LNMA tumor samples.Results:Higher histological grades,aggressive subtypes and more advanced TNM staging were associated with an increased WGCNV score,particularly in CNV regions enriched for tumor suppressor genes and oncogenes.In addition,we demonstrate that 24-chromosome CNV profiling can be performed reliably from specific cell types(<100 cells)isolated by sample laser capture microdissection.Conclusions:Our findings suggest that the WGCNV scoring system we developed may have potential value as an adjunct test for predicting the prognosis of patients diagnosed with LNMA.展开更多
Objective: To investigate the role of miR-34a on the mice knee osteoarthritis.Methods: Mice knee osteoarthritis model was established, and HE staining was used to evaluate the Mankin score of knee cartilage injury. Th...Objective: To investigate the role of miR-34a on the mice knee osteoarthritis.Methods: Mice knee osteoarthritis model was established, and HE staining was used to evaluate the Mankin score of knee cartilage injury. Then the chondrocyte of knee joint was cultured. RT-PCR was used to detect mRNA expression of miR-34a, and Western Blot was used to detect expression of bcl-2 and MMP-13.Results: In the mice knee osteoarthritis model, Mankin score increased, expression of miR-34a and MMP-13 increased, and expression of bcl-2 decreased.Conclusions: In mice knee osteoarthritis model, over-expression of miR-34a leads to the increased expression of MMP-13 and the decreased expression of bcl-2, and promotes osteoarthritis progression. This suggests that silencing miR-34a may become a new method for prevention and treatment of osteoarthritis.展开更多
基金grants from Beijing Hospital Key Research Program(121 Research Program,No.BJ2019-195)。
文摘Objective:Histology grade,subtypes and TNM stage of lung adenocarcinomas are useful predictors of prognosis and survival.The aim of the study was to investigate the relationship between chromosomal instability,morphological subtypes and the grading system used in lung non-mucinous adenocarcinoma(LNMA).Methods:We developed a whole genome copy number variation(WGCNV)scoring system and applied next generation sequencing to evaluate CNVs present in 91 LNMA tumor samples.Results:Higher histological grades,aggressive subtypes and more advanced TNM staging were associated with an increased WGCNV score,particularly in CNV regions enriched for tumor suppressor genes and oncogenes.In addition,we demonstrate that 24-chromosome CNV profiling can be performed reliably from specific cell types(<100 cells)isolated by sample laser capture microdissection.Conclusions:Our findings suggest that the WGCNV scoring system we developed may have potential value as an adjunct test for predicting the prognosis of patients diagnosed with LNMA.
基金Shenzhen Science and Technology Plan Project,No.JCYJ20170307100726777The National Nature Science Foundation of China.Project NO.81272032.
文摘Objective: To investigate the role of miR-34a on the mice knee osteoarthritis.Methods: Mice knee osteoarthritis model was established, and HE staining was used to evaluate the Mankin score of knee cartilage injury. Then the chondrocyte of knee joint was cultured. RT-PCR was used to detect mRNA expression of miR-34a, and Western Blot was used to detect expression of bcl-2 and MMP-13.Results: In the mice knee osteoarthritis model, Mankin score increased, expression of miR-34a and MMP-13 increased, and expression of bcl-2 decreased.Conclusions: In mice knee osteoarthritis model, over-expression of miR-34a leads to the increased expression of MMP-13 and the decreased expression of bcl-2, and promotes osteoarthritis progression. This suggests that silencing miR-34a may become a new method for prevention and treatment of osteoarthritis.
