Rapidly spreading coronavirus disease 2019(COVID-19)is currently affecting the world.Specifically,cytokine storms are a key feature in a substantial num ber of COVID-19 patients,1 and studies from our group and others...Rapidly spreading coronavirus disease 2019(COVID-19)is currently affecting the world.Specifically,cytokine storms are a key feature in a substantial num ber of COVID-19 patients,1 and studies from our group and others suggest that the IL-6/IL-6R cascade plays a dom inant role in symptom-correlated cytokine storms.展开更多
The effects of severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)infection persist months and years after recovery.We conducted an online survey to assess the health condition of convalescents approximately...The effects of severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)infection persist months and years after recovery.We conducted an online survey to assess the health condition of convalescents approximately 5 months following the primary infection of SARS‐CoV‐2.The study recruited 5,510 individuals who were primary infected,626 participants who had experienced reinfection,and 521 participants who were without infective history.The most common disorders after the primary infection group were fatigue(15.18%),memory issue(13.13%),post‐exertional malaise(PEM,11.68%),and brain fog(11.29%)at the time of survey.In addition,SARS‐CoV‐2 infection had an impact on the reproductive systems.In stepwise logistic regression analysis,smoking currently,with background diseases,and outpatient visits in the acute phase could be associated with moderate/severe disorders.Further analysis of different background diseases showed that allergic rhinitis,hyperlipidemia,cardiovascular disease,autoimmune diseases,neurological diseases,and asthma likely increased the risk of moderate/severe disorders.The probability of developing disorders of individuals with SARS‐CoV‐2 reinfection was higher before the secondary infection than uninfected people.Fatigue,PEM,muscle pain/spasms,chills,joint pain,excessive sweating at rest,headache/dizziness,sore throat or foreign body sensation in the throat,cough,expectoration,dry/painful/watery eyes,loss of appetite and constipation were associated with an increased risk of reinfection.It was essential to undertake further research with enhanced randomization in a larger sample in the community,and to strengthen the validation of the research conclusions.The findings of this study contribute to a deeper understanding of the health recovery process among coronavirus disease 2019(COVID‐19)convalescents.Moreover,the findings help identify characteristic health risk factors associated with convalescents and highlight the risk of moderate/severe disorders and reinfection.Furthermore,the findings also provide valuable guidance and reference for SARS‐CoV‐2 rehabilitation strategies and the prevention of reinfection,offering insights for scientific recommendations。展开更多
Seasonal flu,primarily caused by influenza A H1N1 and H3N2 subtype viruses or influenza B viruses,is the most prevalent respiratory tract infection globally and leads to substantial morbidity andmortality annually.Des...Seasonal flu,primarily caused by influenza A H1N1 and H3N2 subtype viruses or influenza B viruses,is the most prevalent respiratory tract infection globally and leads to substantial morbidity andmortality annually.Despite the influenza virus being initially recognized as a respiratory pathogenwithwell-characterized transmission through respiratory droplets,its impact on the ocular epithelium and associated gene expression remains relatively unexplored.In this study,we investigated the transcriptional profiles of immortalized human corneal epithelial cells(HCE-S)and A549 human lung epithelial cells infected with H1N1 and H3N2 influenza virus.In comparison with A549 cells,a reduced number of differentially expressed geneswas observed in HCE-S upon influenza virus infection.Specifically,there was a significant upregulation of the genes IFI44L and OAS1,along with lower release of the CCL5/RANTES protein.Notably,our findings revealed uniquely upregulated LGALS9(encoding galectin-9)in HCE-S following infection with the 2009 pandemic H1N1 virus.Furthermore,targeted knockdown of LGALS9 in these cells resulted in a measurable decrease in viral infection,highlighting its role in the cellular responses to influenza virus and suggesting a novel avenue for antiviral therapy.Overall,our findings provide insight into the distinct mechanisms of influenza virus interactions with different epithelial cells and underscore the importance of studying the ocular surface in understanding influenza pathogenesis.展开更多
Nipah virus(NiV)and Langya virus(LayV)are emerging zoonotic henipaviruses with serious public health risks.However,no vaccine or drug is available for either disease.To address the persistent threats posed by NiV and ...Nipah virus(NiV)and Langya virus(LayV)are emerging zoonotic henipaviruses with serious public health risks.However,no vaccine or drug is available for either disease.To address the persistent threats posed by NiV and LayV,we preliminarily developed indirect ELISAs based on truncated fusion glycoprotein(F)and attachment glycoprotein(G)expressed in a mammalian expression system.We validated these assays using immunized BALB/c mice(for both NiV and LayV),immunized Syrian golden hamsters(for NiV),and goats and a dog that were naturally infected(for LayV).Specificity was assessed using negative sera from mice,ferrets,African green monkeys,hamsters and swine.The ELISAs demonstrated high sensitivity(1:64,000)for both viruses in immunized BALB/c mice,high specificity(approximately 95%in mice and 100%in ferrets,African green monkeys,hamsters and swine),and strong concordance with commercial NiV ELISA kits(>93%).The NiV ELISAs were further validated using immunized Syrian golden hamsters,which had sensitivities of 1:51,200(F-based)and 1:6400(G-based).The LayV ELISAs successfully detected antibodies in the sera of goats and a dog naturally infected with LayV.These preliminary indirect ELISAs serve as proof-of-concept tools and may be valuable for vaccine and therapeutic development,serological surveillance studies and future diagnostic platform development.展开更多
基金supported by CAMS Research Units of Adaptive Evolution and Control of Emerging Viruses(2018RU009)Beijing New-star Plan of Science and Technology(Z181100006218080)+3 种基金supported by the Excellent Young Scientist Program of the National Natural Science Foundation of China(81822040)the National Youth Talent Support Programfunded by the National Natural Science Foundation of China(31900466)funded by the China Postdoctoral Science Foundation(2020T130080ZX).
文摘Rapidly spreading coronavirus disease 2019(COVID-19)is currently affecting the world.Specifically,cytokine storms are a key feature in a substantial num ber of COVID-19 patients,1 and studies from our group and others suggest that the IL-6/IL-6R cascade plays a dom inant role in symptom-correlated cytokine storms.
基金approved by the Ethics Committee of the National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,and informed consent was obtained from the participants(No.IVDC 2023‐005).supported by the National Key Research and Development Program of China(2022YFC2604100,ZDYF‐2023YFC3041500)the National Natural Science Foundation of China(92269203).
文摘The effects of severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)infection persist months and years after recovery.We conducted an online survey to assess the health condition of convalescents approximately 5 months following the primary infection of SARS‐CoV‐2.The study recruited 5,510 individuals who were primary infected,626 participants who had experienced reinfection,and 521 participants who were without infective history.The most common disorders after the primary infection group were fatigue(15.18%),memory issue(13.13%),post‐exertional malaise(PEM,11.68%),and brain fog(11.29%)at the time of survey.In addition,SARS‐CoV‐2 infection had an impact on the reproductive systems.In stepwise logistic regression analysis,smoking currently,with background diseases,and outpatient visits in the acute phase could be associated with moderate/severe disorders.Further analysis of different background diseases showed that allergic rhinitis,hyperlipidemia,cardiovascular disease,autoimmune diseases,neurological diseases,and asthma likely increased the risk of moderate/severe disorders.The probability of developing disorders of individuals with SARS‐CoV‐2 reinfection was higher before the secondary infection than uninfected people.Fatigue,PEM,muscle pain/spasms,chills,joint pain,excessive sweating at rest,headache/dizziness,sore throat or foreign body sensation in the throat,cough,expectoration,dry/painful/watery eyes,loss of appetite and constipation were associated with an increased risk of reinfection.It was essential to undertake further research with enhanced randomization in a larger sample in the community,and to strengthen the validation of the research conclusions.The findings of this study contribute to a deeper understanding of the health recovery process among coronavirus disease 2019(COVID‐19)convalescents.Moreover,the findings help identify characteristic health risk factors associated with convalescents and highlight the risk of moderate/severe disorders and reinfection.Furthermore,the findings also provide valuable guidance and reference for SARS‐CoV‐2 rehabilitation strategies and the prevention of reinfection,offering insights for scientific recommendations。
基金supported by the National Key Research and Development Program of China(2022YFC2604100,2023YFC3041500)the National Natural Science Foundation of China(92269203).
文摘Seasonal flu,primarily caused by influenza A H1N1 and H3N2 subtype viruses or influenza B viruses,is the most prevalent respiratory tract infection globally and leads to substantial morbidity andmortality annually.Despite the influenza virus being initially recognized as a respiratory pathogenwithwell-characterized transmission through respiratory droplets,its impact on the ocular epithelium and associated gene expression remains relatively unexplored.In this study,we investigated the transcriptional profiles of immortalized human corneal epithelial cells(HCE-S)and A549 human lung epithelial cells infected with H1N1 and H3N2 influenza virus.In comparison with A549 cells,a reduced number of differentially expressed geneswas observed in HCE-S upon influenza virus infection.Specifically,there was a significant upregulation of the genes IFI44L and OAS1,along with lower release of the CCL5/RANTES protein.Notably,our findings revealed uniquely upregulated LGALS9(encoding galectin-9)in HCE-S following infection with the 2009 pandemic H1N1 virus.Furthermore,targeted knockdown of LGALS9 in these cells resulted in a measurable decrease in viral infection,highlighting its role in the cellular responses to influenza virus and suggesting a novel avenue for antiviral therapy.Overall,our findings provide insight into the distinct mechanisms of influenza virus interactions with different epithelial cells and underscore the importance of studying the ocular surface in understanding influenza pathogenesis.
基金supported by the National Key Research and Development Program of China(2024YFC2309400)Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-D-202208).
文摘Nipah virus(NiV)and Langya virus(LayV)are emerging zoonotic henipaviruses with serious public health risks.However,no vaccine or drug is available for either disease.To address the persistent threats posed by NiV and LayV,we preliminarily developed indirect ELISAs based on truncated fusion glycoprotein(F)and attachment glycoprotein(G)expressed in a mammalian expression system.We validated these assays using immunized BALB/c mice(for both NiV and LayV),immunized Syrian golden hamsters(for NiV),and goats and a dog that were naturally infected(for LayV).Specificity was assessed using negative sera from mice,ferrets,African green monkeys,hamsters and swine.The ELISAs demonstrated high sensitivity(1:64,000)for both viruses in immunized BALB/c mice,high specificity(approximately 95%in mice and 100%in ferrets,African green monkeys,hamsters and swine),and strong concordance with commercial NiV ELISA kits(>93%).The NiV ELISAs were further validated using immunized Syrian golden hamsters,which had sensitivities of 1:51,200(F-based)and 1:6400(G-based).The LayV ELISAs successfully detected antibodies in the sera of goats and a dog naturally infected with LayV.These preliminary indirect ELISAs serve as proof-of-concept tools and may be valuable for vaccine and therapeutic development,serological surveillance studies and future diagnostic platform development.
