DNA methyltransferase 1(DNMT1)is a useful biomarker for lung cancer in early clinical diagnosis.A rapid magnetic chemiluminescence immunoassay(MCLIA)for DNMT1 in human serum has been developed.Horseradish peroxidase(H...DNA methyltransferase 1(DNMT1)is a useful biomarker for lung cancer in early clinical diagnosis.A rapid magnetic chemiluminescence immunoassay(MCLIA)for DNMT1 in human serum has been developed.Horseradish peroxidase(HRP)-second-Ab was used to labeled polyclonal antibodies of anti-DNMT1.DNMT1 in sample integrates with specific immunomagnetic beads and can constitute a supersandwiched immunoreaction.In magnetic field,nonspecific materials can be separated.After luminescent substrate luminol-H2O2-BIP was added,the relative light unit(RLU)of HRP was detected and was discovered to be directly proportional to the content of DNMT1 in sample.The correlative variables involved in the MCLIA value were optimized and the methodological evaluation was carried out.After optimization,in the range of0.5–128 ng/mL,the linear regression equation was y=0.5014 x+1.769(x was logCDNMT1,y was relative luminescence units(RLU)/RLU0),and the limit of detection was 0.01 ng/mL.The RSD of intra-and interassays were 15.8%–16.9%and 14.3%–18.1%,respectively.The recovery was from 70.0%to 106.2%.Furthermore,paralleled with purchasable enzyme-linked immunosorbent assay(ELISA)kits,MCLEIA had lower detection limit,wider linear range and shorter detection time.Therefore,the MCLEIA established in this study could be used for the sensitive detection of DNMT1 in serum sample.展开更多
The characterization of exoplanets and their birth protoplanetary disks has enormously advanced in the last decade.Benefitting from that,our global understanding of the planet formation processes has been substantiall...The characterization of exoplanets and their birth protoplanetary disks has enormously advanced in the last decade.Benefitting from that,our global understanding of the planet formation processes has been substantially improved.In this review,we first summarize the cutting-edge states of the exoplanet and disk observations.We further present a comprehensive panoptic view of modern core accretion planet formation scenarios,including dust growth and radial drift,planetesimal formation by the streaming instability,core growth by planetesimal accretion and pebble accretion.We discuss the key concepts and physical processes in each growth stage and elaborate on the connections between theoretical studies and observational revelations.Finally,we point out the critical questions and future directions of planet formation studies.展开更多
With the increase in the need for flood prevention and lake resource used by humans,the construction of floodgates and sluices has changed the hydrological connection between rivers and lakes,and between adjacent lake...With the increase in the need for flood prevention and lake resource used by humans,the construction of floodgates and sluices has changed the hydrological connection between rivers and lakes,and between adjacent lakes.In river-disconnected lakes,exploitation and use of lake resources have resulted in water quality decline and mechanical disturbance intensification to a different degree.Of the large number of river-disconnected lakes in the middle-lower reaches of the Changjiang(Yangtze)River,the Futou Lake,and the Xiliang Lake lie close together and are,historically,directly connected,and so do Liangzi Lake and Baoan Lake.The extent of human disturbance is severe in the Futou Lake and the Baoan Lake,but relatively mild in the Xiliang Lake and Liangzi Lake.The freshwater rosette-forming submerged plant Vallisneria natans is one of the dominant species in the four lakes.Using microsatellite markers,we studied the genetic variation of V.natans subpopulations in lakes with different intensities of human disturbance and historical direct hydrological connections.Our results showed that human disturbance decreased plant density and clonal growth in V.natans,but might increase genetic and clonal diversity at a subpopulation level and enhance gene flow among subpopulations by sexual propagule movement.Under similar climatic conditions,different intensities of disturbance seem to have such a high selective potential to differentiate genetically adjacent lake populations that they outperform the forces of gene flow through historical direct hydrological interconnection,which tends to produce genetic homogeneity.Our findings imply that human disturbance has a profound eff ect on the evolutionary process of natural populations of submerged plants.Moreover,increased subpopulation genetic diversity can enhance resistance and resilience to environmental disturbances.To a certain degree,we could expect that disturbed populations have the possibility of restoring spontaneously if humans cease to perturb natural ecosystems in the future.展开更多
Objective Evidence on potential cardiovascular benefits of personal-level intervention among the elderly exposed to high levels of particulate matter(PM)remains limited.We aimed to assess improvements in surrogate mar...Objective Evidence on potential cardiovascular benefits of personal-level intervention among the elderly exposed to high levels of particulate matter(PM)remains limited.We aimed to assess improvements in surrogate markers of cardiovascular injury in vulnerable populations at risks by using indoor air filtration units.Methods We conducted a randomized crossover trial for 2 separate 2-week air filtration interventions in 20 households of patients with stable chronic obstructive pulmonary disease and their partners in the winter of 2013,with concurrent measurements of indoor PM.The changes in biomarkers indicative of cardiac injury,atherosclerosis progression and systemic inflammation following intervention were evaluated using linear mixed-effect models.Results In the analysis,average levels of indoor PM with aerodynamic diameters<2.5µm(PM2.5)decreased significantly by 59.2%(from 59.6 to 24.3µg/m3,P<0.001)during the active air filtration.The reduction was accompanied by improvements in levels of high-sensitivity cardiac troponin I by−84.6%(95%confidence interval[CI]:−90.7 to−78.6),growth differentiation factor-15 by−48.1%(95%CI:−31.2 to−25.6),osteoprotegerin by−65.4%(95%CI:−56.5 to−18.7),interleukin-4 by−46.6%(95%CI:−62.3 to−31.0)and myeloperoxidase by−60.3%(95%CI:−83.7 to−3.0),respectively.Conclusion Indoor air filtration intervention may provide potential cardiovascular benefits in vulnerable populations at risks.展开更多
Polycyclic aromatic hydrocarbons (PAHs) pollution in mangroves has drawn much attention, but knowledge of the sorption of PAHs in mangrove sediment is limited. This study investigated the particles and water-stable ag...Polycyclic aromatic hydrocarbons (PAHs) pollution in mangroves has drawn much attention, but knowledge of the sorption of PAHs in mangrove sediment is limited. This study investigated the particles and water-stable aggregates (WSA) of mangrove sediment in Jiulong River Estuary, China, and the characteristics of anthracene adsorption to them. The adsorption of anthracene was strongly influenced by the physicochemical and structural properties of sediment particles and WSA. The main sorbents of mangrove sediment were carbonized particles and clays. The porous structure of carbonized particles made it easy to sequestrate sequester the anthracene, and the aging allowed anthracene to move into deeper sites of the carbonized particles. Clays had high anthracene-fixing capacities, and they included organic matters and formed aggregates. The sorption contents coefficient <i>K<sub>f</sub></i> of anthracene with WSA of different sizes increased in the order 0.063 - 0.25 mm > 0.063 mm > 0.25 - 1.0 mm > 1.0 mm. The order was correlated with which due to the contents and characteristics of organic matters in the aggregates.展开更多
Cervical cancer is one of the most common malignant gynecological tumors and has the second highest incidence of all malignancies in females.Chronic and persistent infection with High Risk Human Papillomavirus(HR-HPV)...Cervical cancer is one of the most common malignant gynecological tumors and has the second highest incidence of all malignancies in females.Chronic and persistent infection with High Risk Human Papillomavirus(HR-HPV)is the main cause of cervical cancer.There is a distinct lack of methodology by which to determine whether cervical epithelial dysplasia is cancerous following HPV infection.HPV L1 capsid protein is a major structural protein of human papillomavirus(HPV),and it is the main target of the local cellular immune response aiming to combat human papillomavirus after HPV infection within cervical cells.Greater understanding of HPV L1 capsid protein and its association with cervical cytology,histopathology,patient age and human papillomavirus viral load has the potential to contribute toward improved the diagnosis and management of cervical cancer,providing useful information for gynecological clinicians in the hope of improving patient treatment and quality of life.This article reviews the predictive utility of HPV L1 capsid protein for cervical lesions.展开更多
Silicone is a kind of polymer material with high cross-linked structure,which is com-posed by Si-O-Si main chain.Due to the special molecular chain structure,silicone mate-rials are characterized by oxidation resistan...Silicone is a kind of polymer material with high cross-linked structure,which is com-posed by Si-O-Si main chain.Due to the special molecular chain structure,silicone mate-rials are characterized by oxidation resistance,aging resistance,high and low temperature resistance and chemical corrosion resistance.Moreover,silicone materials have process-able properties,simple forming process,good mechanical property,non-toxic and pollution-free.Therefore,silicone has been widely concerned by researchers at home and abroad.In this paper,the main research progress and application directions of carbon-silicone composite at home and abroad in recent years are reviewed.展开更多
Introduction:Public health surveillance is crucial for decision-making.Given the significant threat of influenza to public health,developing predictive models using multichannel surveillance systems is imperative.Meth...Introduction:Public health surveillance is crucial for decision-making.Given the significant threat of influenza to public health,developing predictive models using multichannel surveillance systems is imperative.Methods:Data were collected from multichannel surveillance systems,including hospitals,search engines,and climatological and air pollutant surveillance systems,in a southern Chinese city from January 2023 to January 2025.Spearman’s correlation analysis assessed the relationships between variables and reported influenza cases.Several machine learning models were used to predict trends in reported cases.Results:Correlation analysis showed that all four surveillance systems were related to influenza,with 27 variables correlated with daily reported cases.The Long Short-Term Memory model,established based on variables with the highest lagged correlations(5-day to 7-day lag)through combined surveillance systems,outperformed other models for 5-day forecasts(R2=0.92;mean absolute error=156.92;mean absolute percentage error=79.95%;root Mean Squared Error=292.33).Conclusions:Data from various surveillance systems effectively track influenza epidemics.The model shows potential for infectious disease surveillance and epidemic preparedness.展开更多
Vitality transfer patterns are essential for creating vibrant,sustainable cities,yet their dynamic changes over time remain underexplored.Taking Nanjing as a case study,this study employed 24 h of location-based servi...Vitality transfer patterns are essential for creating vibrant,sustainable cities,yet their dynamic changes over time remain underexplored.Taking Nanjing as a case study,this study employed 24 h of location-based service data as a time series to explore the vitality transfer pattern within a day from both distribution and aggregation perspectives.Spatial dependence decay patterns were detected using residual clustering relationships,and the LightGBM model was used to explore the relationship between vitality transfer and 50 factors in five categories:transportation,function,economy,morphology,and geography.The results show that the urban vitality distribution has a polycentric agglomeration pattern,which goes through four periods in a day.Vitality transfer is the cyclical process of transformation from one aggregated state to another.The spatial dependence was maximized at 0.75 km^(2).The magnitude of vitality fluctuation is strongly influenced by factors such as morphology,transportation,and function.Spatial differences in factors combine to drive vitality transfer in distribution and aggregation,with factors such as accessibility and building age diversity influencing distribution,and factors such as accessibility and POI diversity altering aggregation.This study supports the rational design of vibrant urban spaces and promotes effective vitality transfer and sustainable urban development.展开更多
Aging is characterized by a progressive deterioration of physiological integrity,leading to impaired functional ability and ultimately increased susceptibility to death.It is a major risk factor for chronic human dise...Aging is characterized by a progressive deterioration of physiological integrity,leading to impaired functional ability and ultimately increased susceptibility to death.It is a major risk factor for chronic human diseases,including cardiovascular disease,diabetes,neurological degeneration,and cancer.Therefore,the growing emphasis on “healthy aging” raises a series of important questions in life and social sciences.In recent years,there has been unprecedented progress in aging research,particularly the discovery that the rate of aging is at least partly controlled by evolutionarily conserved genetic pathways and biological processes.In an attempt to bring full-fledged understanding to both the aging process and age-associated diseases,we review the descriptive,conceptual,and interventive aspects of the landscape of aging composed of a number of layers at the cellular,tissue,organ,organ system,and organismal levels.展开更多
While deltas fed by seasonal rivers are common in modem sedimentary environments,their characteristics remain unclear as compared to those fed by perennial rivers.This study identifies a small delta discharged by a se...While deltas fed by seasonal rivers are common in modem sedimentary environments,their characteristics remain unclear as compared to those fed by perennial rivers.This study identifies a small delta discharged by a seasonal stream flowing into Daihai Lake,in northern China,which is driven by ephemeral and high-energy flood events.Detailed 3D facies architecture was analyzed using ground-penetrating radar (GPR) and sedimentary logs from outcrop and trenches.Four types of radar surfaces,including truncations of underlying inclined strata,weak reflections,and depositional surface of downlap and onlap,were identified.Six radar facies (highangle obliquetangential,low-angle subparallel,gently plane parallel,plane-parallel,chaotic,and continuous strong reflection) were identified based on distinctive reflections,including amplitude,continuity,dip,and termination patterns.Five depositional units (Unit A to E) were documented from proximal to distal delta.Seasonal discharge signatures include significant grain-size decrease over short distance,abundant Froude supercritical flow sedimentary structures,poorly developed barforms,and small-scale scour and fill structures.Records of lake-level and sediment budget were evaluated over the past 60 years.In highstand stage (1960- 1980),amalgamated channel (Units A and B),and delta front (Unit C) were deposited.In slope stage (1980-1996),the lower deposits (Units A,B,C) were eroded by Unit D with a distinct truncation surface.In lowstand stage,most eroded sediments bypassed the incised channel and accumulated in the distal part,in which a new depositional unit was formed (Unit E).The model demonstrates that deltas fed by seasonal rivers tend to accumulate large amounts of sediments carried by high magnitude floods within short periods.展开更多
The electrical conductivity(EC)of extracted muscle fluid has been extensively used to evaluate meat freshness and shelf life in the field of food sanitation for decades.The opposite of freshness is the corruption that...The electrical conductivity(EC)of extracted muscle fluid has been extensively used to evaluate meat freshness and shelf life in the field of food sanitation for decades.The opposite of freshness is the corruption that increases with time.Based on the freshness/corruption principle,we investigated the relationship between long postmortem intervals(PMIs)and EC in cadaver skeletal muscle.EC values of extracted fluid from rat muscles were measured at different PMIs for 10 days.The results indicate that there was a significant correlation between PMI and EC,and the data fit well to the cubic polynomial regression equation y=‑0.01x3+0.264x2‑13.657x+1769.148(R2=0.925).In addition,the EC of different dilutions of these muscle extracts showed strict quadratic correlation(R2=1)with the dilution ratios,suggesting that EC can be measured with very small quantities of muscle sample.Our study suggests that determination of the EC of cadaver skeletal muscle extracts may be a useful method for estimating long PMIs.展开更多
Ammonium(NH_(4)^(+))and nitrate(NO_(3)^(-))are major inorganic nitrogen(N)sources for plants.When serving as the sole or dominant N supply,NH_(4)^(+)often causes root inhibition and shoot chlorosis in plants,known as ...Ammonium(NH_(4)^(+))and nitrate(NO_(3)^(-))are major inorganic nitrogen(N)sources for plants.When serving as the sole or dominant N supply,NH_(4)^(+)often causes root inhibition and shoot chlorosis in plants,known as ammonium toxicity.NO_(3)^(-) usually causes no toxicity and can mitigate ammonium toxicity even at low concentrations,referred to as nitrate-dependent alleviation of ammonium toxicity.Our previous studies indicated a NO_(3)^(-) efflux channel SLAH3 is involved in this process.However,whether additional components contribute to NO_(3)^(-)-mediated NH_(4)^(+)detoxification is unknown.Previously,mutations in NO_(3)^(-) transporter NRT1.1 were shown to cause enhanced resistance to high concentrations of NH_(4)^(+).Whereas,in this study,we found when the high-NH_(4)^(+) medium was supplemented with low concentrations of NO_(3)^(-),nrt1.1 mutant plants showed hyper-sensitive phenotype instead.Furthermore,mutation in NRT1.1 caused enhanced medium acidification under high-NH_(4)^(+)/Iow-NO_(3)^(-) condition,suggesting NRT1.1 regulates ammonium toxicity by facilitating H+uptake.Moreover,NRT1.1 was shown to interact with SLAH3 to form a transporter-channel complex.Interestingly,SLAH3 appeared to affect NO_(3)^(-) influx while NRT1.1 influenced NO_(3)^(-) efflux,suggesting NRT1.1 and SLAH3 regulate each other at protein and/or gene expression levels.Our study thus revealed NRT1.1 and SLAH3 form a functional unit to regulate nitrate-dependent alleviation of ammonium toxicity through regulating NO_(3)^(-) transport and balancing rhizosphere acidification.展开更多
The prognosis of glioblastoma(GBM)remains challenging,primarily due to the lack of a precise,effective imaging technique for comprehensively characterization.Addressing GBM diagnostic challenges,our study introduces a...The prognosis of glioblastoma(GBM)remains challenging,primarily due to the lack of a precise,effective imaging technique for comprehensively characterization.Addressing GBM diagnostic challenges,our study introduces an innovative dual-modal imaging that merges near-infrared(NIR)fluorescent imaging with magnetic resonance imaging(MR).This method employs superparamagnetic iron oxide nanoparticies coated with NIR fluorescent dyes,specifically Cyanine 7,and targeted peptides.This synthetic probe facilitates MRI functionality through superparamagnetic iron oxide nanoparticles,provides NIR imaging capability via Cyanine 7 and enhances tumor targeting trough peptide interactions,offering a comprehensive diagnostic tool for GBM.Notably,the probe traverses the blood-brain barrier,targeting GBM in vivo via peptides,producing clear and discermible images in both modalities.Cytotoxicity and histopathology assessments confirm the probe's favorable safet profile.These findings suggest that the dual-modal MRINIR fluorescent imaging probe could revolutionize GBM prognosis and survival rate which can also be extended to other tumors type.展开更多
Dear Editor, N6-methyladenosine(m6A)is an abundant epitranscriptomic modification that regulates messenger RNA(mRNA)biology.The m6A modification regulates mRNA splicing,transport,stability,and translation through coor...Dear Editor, N6-methyladenosine(m6A)is an abundant epitranscriptomic modification that regulates messenger RNA(mRNA)biology.The m6A modification regulates mRNA splicing,transport,stability,and translation through coordinated activities by methyltransferases(writers),binding proteins(readers),and demethylases(erasers)(Huang et al.,2020;Wu et al.,2020).Among m6A regulators,fat mass of obesity-associ-ated protein(FTO),is the first discovered eraser with RNA m6A demethylation activity(Jia et al.,2011).Since then,FTO has been reported to play m6A-dependent roles in a variety of physiological processes including adipogenesis,neuro-genesis and tumorigenesis(Fischer et al.,2009;Li et al.,2017;Huang et al.,2020).Consequently,FTO deficiency in mice leads to dramatic phenotypes,such as decreased fat mass and impaired brain development(Fischer et al.,2009;Li et al.,2017).Similarly,inhibition of FTO reduces tumori-genesis in multiple types of cancer models,while FTO is highly expressed in many cancers(Huang et al.,2020).展开更多
基金supported by the National Natural Science Foundation of China(Nos.81402721,81573203,21605131)Science and Technology Department of Henan Province(No.22170004)
文摘DNA methyltransferase 1(DNMT1)is a useful biomarker for lung cancer in early clinical diagnosis.A rapid magnetic chemiluminescence immunoassay(MCLIA)for DNMT1 in human serum has been developed.Horseradish peroxidase(HRP)-second-Ab was used to labeled polyclonal antibodies of anti-DNMT1.DNMT1 in sample integrates with specific immunomagnetic beads and can constitute a supersandwiched immunoreaction.In magnetic field,nonspecific materials can be separated.After luminescent substrate luminol-H2O2-BIP was added,the relative light unit(RLU)of HRP was detected and was discovered to be directly proportional to the content of DNMT1 in sample.The correlative variables involved in the MCLIA value were optimized and the methodological evaluation was carried out.After optimization,in the range of0.5–128 ng/mL,the linear regression equation was y=0.5014 x+1.769(x was logCDNMT1,y was relative luminescence units(RLU)/RLU0),and the limit of detection was 0.01 ng/mL.The RSD of intra-and interassays were 15.8%–16.9%and 14.3%–18.1%,respectively.The recovery was from 70.0%to 106.2%.Furthermore,paralleled with purchasable enzyme-linked immunosorbent assay(ELISA)kits,MCLEIA had lower detection limit,wider linear range and shorter detection time.Therefore,the MCLEIA established in this study could be used for the sensitive detection of DNMT1 in serum sample.
基金supported by the European Research Council(ERC Consolidator Grant 724687-PLANETESYS)the Swedish Walter Gyllenberg Foundation+3 种基金start-up grant of Bairen program from Zhejiang Universitysupported by the B-type Strategic Priority Program of the Chinese Academy of Sciences(Grant No.XDB41000000)the National Natural Science Foundation of China(Grant Nos.12033010 and 11773081)CAS Interdisciplinary Innovation Team and Foundation of Minor Planets of the Purple Mountain Observatory。
文摘The characterization of exoplanets and their birth protoplanetary disks has enormously advanced in the last decade.Benefitting from that,our global understanding of the planet formation processes has been substantially improved.In this review,we first summarize the cutting-edge states of the exoplanet and disk observations.We further present a comprehensive panoptic view of modern core accretion planet formation scenarios,including dust growth and radial drift,planetesimal formation by the streaming instability,core growth by planetesimal accretion and pebble accretion.We discuss the key concepts and physical processes in each growth stage and elaborate on the connections between theoretical studies and observational revelations.Finally,we point out the critical questions and future directions of planet formation studies.
基金Supported by the National Natural Science Foundation of China(No.31600325)the self-determined research funds of the Central China Normal University(CCNU)from the Colleges’Basic Research and Operation of MOE(No.CCNU20TS022)。
文摘With the increase in the need for flood prevention and lake resource used by humans,the construction of floodgates and sluices has changed the hydrological connection between rivers and lakes,and between adjacent lakes.In river-disconnected lakes,exploitation and use of lake resources have resulted in water quality decline and mechanical disturbance intensification to a different degree.Of the large number of river-disconnected lakes in the middle-lower reaches of the Changjiang(Yangtze)River,the Futou Lake,and the Xiliang Lake lie close together and are,historically,directly connected,and so do Liangzi Lake and Baoan Lake.The extent of human disturbance is severe in the Futou Lake and the Baoan Lake,but relatively mild in the Xiliang Lake and Liangzi Lake.The freshwater rosette-forming submerged plant Vallisneria natans is one of the dominant species in the four lakes.Using microsatellite markers,we studied the genetic variation of V.natans subpopulations in lakes with different intensities of human disturbance and historical direct hydrological connections.Our results showed that human disturbance decreased plant density and clonal growth in V.natans,but might increase genetic and clonal diversity at a subpopulation level and enhance gene flow among subpopulations by sexual propagule movement.Under similar climatic conditions,different intensities of disturbance seem to have such a high selective potential to differentiate genetically adjacent lake populations that they outperform the forces of gene flow through historical direct hydrological interconnection,which tends to produce genetic homogeneity.Our findings imply that human disturbance has a profound eff ect on the evolutionary process of natural populations of submerged plants.Moreover,increased subpopulation genetic diversity can enhance resistance and resilience to environmental disturbances.To a certain degree,we could expect that disturbed populations have the possibility of restoring spontaneously if humans cease to perturb natural ecosystems in the future.
基金This work was supported by Beijing Natural Science Foundation(7222246)Royal Dutch Philips Electronics Ltd.(Grant NL18-2100478471).
文摘Objective Evidence on potential cardiovascular benefits of personal-level intervention among the elderly exposed to high levels of particulate matter(PM)remains limited.We aimed to assess improvements in surrogate markers of cardiovascular injury in vulnerable populations at risks by using indoor air filtration units.Methods We conducted a randomized crossover trial for 2 separate 2-week air filtration interventions in 20 households of patients with stable chronic obstructive pulmonary disease and their partners in the winter of 2013,with concurrent measurements of indoor PM.The changes in biomarkers indicative of cardiac injury,atherosclerosis progression and systemic inflammation following intervention were evaluated using linear mixed-effect models.Results In the analysis,average levels of indoor PM with aerodynamic diameters<2.5µm(PM2.5)decreased significantly by 59.2%(from 59.6 to 24.3µg/m3,P<0.001)during the active air filtration.The reduction was accompanied by improvements in levels of high-sensitivity cardiac troponin I by−84.6%(95%confidence interval[CI]:−90.7 to−78.6),growth differentiation factor-15 by−48.1%(95%CI:−31.2 to−25.6),osteoprotegerin by−65.4%(95%CI:−56.5 to−18.7),interleukin-4 by−46.6%(95%CI:−62.3 to−31.0)and myeloperoxidase by−60.3%(95%CI:−83.7 to−3.0),respectively.Conclusion Indoor air filtration intervention may provide potential cardiovascular benefits in vulnerable populations at risks.
文摘Polycyclic aromatic hydrocarbons (PAHs) pollution in mangroves has drawn much attention, but knowledge of the sorption of PAHs in mangrove sediment is limited. This study investigated the particles and water-stable aggregates (WSA) of mangrove sediment in Jiulong River Estuary, China, and the characteristics of anthracene adsorption to them. The adsorption of anthracene was strongly influenced by the physicochemical and structural properties of sediment particles and WSA. The main sorbents of mangrove sediment were carbonized particles and clays. The porous structure of carbonized particles made it easy to sequestrate sequester the anthracene, and the aging allowed anthracene to move into deeper sites of the carbonized particles. Clays had high anthracene-fixing capacities, and they included organic matters and formed aggregates. The sorption contents coefficient <i>K<sub>f</sub></i> of anthracene with WSA of different sizes increased in the order 0.063 - 0.25 mm > 0.063 mm > 0.25 - 1.0 mm > 1.0 mm. The order was correlated with which due to the contents and characteristics of organic matters in the aggregates.
文摘Cervical cancer is one of the most common malignant gynecological tumors and has the second highest incidence of all malignancies in females.Chronic and persistent infection with High Risk Human Papillomavirus(HR-HPV)is the main cause of cervical cancer.There is a distinct lack of methodology by which to determine whether cervical epithelial dysplasia is cancerous following HPV infection.HPV L1 capsid protein is a major structural protein of human papillomavirus(HPV),and it is the main target of the local cellular immune response aiming to combat human papillomavirus after HPV infection within cervical cells.Greater understanding of HPV L1 capsid protein and its association with cervical cytology,histopathology,patient age and human papillomavirus viral load has the potential to contribute toward improved the diagnosis and management of cervical cancer,providing useful information for gynecological clinicians in the hope of improving patient treatment and quality of life.This article reviews the predictive utility of HPV L1 capsid protein for cervical lesions.
文摘Silicone is a kind of polymer material with high cross-linked structure,which is com-posed by Si-O-Si main chain.Due to the special molecular chain structure,silicone mate-rials are characterized by oxidation resistance,aging resistance,high and low temperature resistance and chemical corrosion resistance.Moreover,silicone materials have process-able properties,simple forming process,good mechanical property,non-toxic and pollution-free.Therefore,silicone has been widely concerned by researchers at home and abroad.In this paper,the main research progress and application directions of carbon-silicone composite at home and abroad in recent years are reviewed.
基金Supported by the National Key Research and Development Program of China(grant no.2022YFC2305103).
文摘Introduction:Public health surveillance is crucial for decision-making.Given the significant threat of influenza to public health,developing predictive models using multichannel surveillance systems is imperative.Methods:Data were collected from multichannel surveillance systems,including hospitals,search engines,and climatological and air pollutant surveillance systems,in a southern Chinese city from January 2023 to January 2025.Spearman’s correlation analysis assessed the relationships between variables and reported influenza cases.Several machine learning models were used to predict trends in reported cases.Results:Correlation analysis showed that all four surveillance systems were related to influenza,with 27 variables correlated with daily reported cases.The Long Short-Term Memory model,established based on variables with the highest lagged correlations(5-day to 7-day lag)through combined surveillance systems,outperformed other models for 5-day forecasts(R2=0.92;mean absolute error=156.92;mean absolute percentage error=79.95%;root Mean Squared Error=292.33).Conclusions:Data from various surveillance systems effectively track influenza epidemics.The model shows potential for infectious disease surveillance and epidemic preparedness.
基金supported by the National Natural Science Foundation of China(Grant No.52078307).
文摘Vitality transfer patterns are essential for creating vibrant,sustainable cities,yet their dynamic changes over time remain underexplored.Taking Nanjing as a case study,this study employed 24 h of location-based service data as a time series to explore the vitality transfer pattern within a day from both distribution and aggregation perspectives.Spatial dependence decay patterns were detected using residual clustering relationships,and the LightGBM model was used to explore the relationship between vitality transfer and 50 factors in five categories:transportation,function,economy,morphology,and geography.The results show that the urban vitality distribution has a polycentric agglomeration pattern,which goes through four periods in a day.Vitality transfer is the cyclical process of transformation from one aggregated state to another.The spatial dependence was maximized at 0.75 km^(2).The magnitude of vitality fluctuation is strongly influenced by factors such as morphology,transportation,and function.Spatial differences in factors combine to drive vitality transfer in distribution and aggregation,with factors such as accessibility and building age diversity influencing distribution,and factors such as accessibility and POI diversity altering aggregation.This study supports the rational design of vibrant urban spaces and promotes effective vitality transfer and sustainable urban development.
基金supported by the National Natural Science Foundation of China(31871380,32000500,32070730,32170756,32170804,81330008,81671377,81725010,81725010,81872874,81921006,81922027,81971312,81991512,82030041,82103167,82122024,82125009,82125011,82130044,91749126,91949101,91949207,92049302)the National Key Research and Development Program of China(2017YFA0506400,2018YFA0800200,2018YFA0800700,2018YFA0900200,2018YFC2000100,2018YFC2000400,2018YFE-0203700,20192ACB70002,2019YFA0802202,2020YFA0113400,2020YFA0803401,2020YFA0804000,2020YFC2002800,2020YFC-2002900,2021ZD0202401)+11 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16010100,XDA16010603,XDA16020400,XDB29020000,XDB39000000,XDB39000000,XDB39030300)the China Association for Science and Technology(2021QNRC001)the Beijing Municipal Science and Technology Commission(Z200022)the Natural Science Foundation of Shanghai(21JC1406400)the Key Programs of the Jiangxi ProvinceChina(20192ACB70002)the“Shu Guang”Project supported by the Shanghai Municipal Education Commission and Shanghai Education Development Foundation(19SG18)the Shanghai Sailing Program(22YF1434300)the Research Project of Joint Laboratory of University of Science and Technology of China and Anhui Mental Health Center(2019LH03)the Fundamental Research Funds for the Central Universities(WK2070210004)the Young Elite Scientists Sponsorship Program by China Association for Science and Technology(YESS20210002)the Youth Innovation Promotion Association of Chinese Academy of Sciences(2022083)。
文摘Aging is characterized by a progressive deterioration of physiological integrity,leading to impaired functional ability and ultimately increased susceptibility to death.It is a major risk factor for chronic human diseases,including cardiovascular disease,diabetes,neurological degeneration,and cancer.Therefore,the growing emphasis on “healthy aging” raises a series of important questions in life and social sciences.In recent years,there has been unprecedented progress in aging research,particularly the discovery that the rate of aging is at least partly controlled by evolutionarily conserved genetic pathways and biological processes.In an attempt to bring full-fledged understanding to both the aging process and age-associated diseases,we review the descriptive,conceptual,and interventive aspects of the landscape of aging composed of a number of layers at the cellular,tissue,organ,organ system,and organismal levels.
基金the National Natural Science Foundation of China (Grant No.41602117)the opening foundation of the Key Laboratory of Marine Sedimentology & Environmental Geology,SOA (No.MASEG201706).
文摘While deltas fed by seasonal rivers are common in modem sedimentary environments,their characteristics remain unclear as compared to those fed by perennial rivers.This study identifies a small delta discharged by a seasonal stream flowing into Daihai Lake,in northern China,which is driven by ephemeral and high-energy flood events.Detailed 3D facies architecture was analyzed using ground-penetrating radar (GPR) and sedimentary logs from outcrop and trenches.Four types of radar surfaces,including truncations of underlying inclined strata,weak reflections,and depositional surface of downlap and onlap,were identified.Six radar facies (highangle obliquetangential,low-angle subparallel,gently plane parallel,plane-parallel,chaotic,and continuous strong reflection) were identified based on distinctive reflections,including amplitude,continuity,dip,and termination patterns.Five depositional units (Unit A to E) were documented from proximal to distal delta.Seasonal discharge signatures include significant grain-size decrease over short distance,abundant Froude supercritical flow sedimentary structures,poorly developed barforms,and small-scale scour and fill structures.Records of lake-level and sediment budget were evaluated over the past 60 years.In highstand stage (1960- 1980),amalgamated channel (Units A and B),and delta front (Unit C) were deposited.In slope stage (1980-1996),the lower deposits (Units A,B,C) were eroded by Unit D with a distinct truncation surface.In lowstand stage,most eroded sediments bypassed the incised channel and accumulated in the distal part,in which a new depositional unit was formed (Unit E).The model demonstrates that deltas fed by seasonal rivers tend to accumulate large amounts of sediments carried by high magnitude floods within short periods.
文摘The electrical conductivity(EC)of extracted muscle fluid has been extensively used to evaluate meat freshness and shelf life in the field of food sanitation for decades.The opposite of freshness is the corruption that increases with time.Based on the freshness/corruption principle,we investigated the relationship between long postmortem intervals(PMIs)and EC in cadaver skeletal muscle.EC values of extracted fluid from rat muscles were measured at different PMIs for 10 days.The results indicate that there was a significant correlation between PMI and EC,and the data fit well to the cubic polynomial regression equation y=‑0.01x3+0.264x2‑13.657x+1769.148(R2=0.925).In addition,the EC of different dilutions of these muscle extracts showed strict quadratic correlation(R2=1)with the dilution ratios,suggesting that EC can be measured with very small quantities of muscle sample.Our study suggests that determination of the EC of cadaver skeletal muscle extracts may be a useful method for estimating long PMIs.
基金supported by the National Natural Science Foundation of China(31870235,32170280)the 111 Project(B16022)the Fundamental Research Funds for the Central Universities(lzujbky-2019-kb05,lzujbky-2020-kb05,lzujbky-2021-it22)。
文摘Ammonium(NH_(4)^(+))and nitrate(NO_(3)^(-))are major inorganic nitrogen(N)sources for plants.When serving as the sole or dominant N supply,NH_(4)^(+)often causes root inhibition and shoot chlorosis in plants,known as ammonium toxicity.NO_(3)^(-) usually causes no toxicity and can mitigate ammonium toxicity even at low concentrations,referred to as nitrate-dependent alleviation of ammonium toxicity.Our previous studies indicated a NO_(3)^(-) efflux channel SLAH3 is involved in this process.However,whether additional components contribute to NO_(3)^(-)-mediated NH_(4)^(+)detoxification is unknown.Previously,mutations in NO_(3)^(-) transporter NRT1.1 were shown to cause enhanced resistance to high concentrations of NH_(4)^(+).Whereas,in this study,we found when the high-NH_(4)^(+) medium was supplemented with low concentrations of NO_(3)^(-),nrt1.1 mutant plants showed hyper-sensitive phenotype instead.Furthermore,mutation in NRT1.1 caused enhanced medium acidification under high-NH_(4)^(+)/Iow-NO_(3)^(-) condition,suggesting NRT1.1 regulates ammonium toxicity by facilitating H+uptake.Moreover,NRT1.1 was shown to interact with SLAH3 to form a transporter-channel complex.Interestingly,SLAH3 appeared to affect NO_(3)^(-) influx while NRT1.1 influenced NO_(3)^(-) efflux,suggesting NRT1.1 and SLAH3 regulate each other at protein and/or gene expression levels.Our study thus revealed NRT1.1 and SLAH3 form a functional unit to regulate nitrate-dependent alleviation of ammonium toxicity through regulating NO_(3)^(-) transport and balancing rhizosphere acidification.
基金the Scientific and Technological Achievements Transformation Fund of West China Hospital,Sichuan University(grant CGZH21002)the Innovative Research Project of Sichuan University(grant 2022SCUH00132)the Sichuan Foundation for Distinguished Young Scholars(grant 2022JDJQ0049).
文摘The prognosis of glioblastoma(GBM)remains challenging,primarily due to the lack of a precise,effective imaging technique for comprehensively characterization.Addressing GBM diagnostic challenges,our study introduces an innovative dual-modal imaging that merges near-infrared(NIR)fluorescent imaging with magnetic resonance imaging(MR).This method employs superparamagnetic iron oxide nanoparticies coated with NIR fluorescent dyes,specifically Cyanine 7,and targeted peptides.This synthetic probe facilitates MRI functionality through superparamagnetic iron oxide nanoparticles,provides NIR imaging capability via Cyanine 7 and enhances tumor targeting trough peptide interactions,offering a comprehensive diagnostic tool for GBM.Notably,the probe traverses the blood-brain barrier,targeting GBM in vivo via peptides,producing clear and discermible images in both modalities.Cytotoxicity and histopathology assessments confirm the probe's favorable safet profile.These findings suggest that the dual-modal MRINIR fluorescent imaging probe could revolutionize GBM prognosis and survival rate which can also be extended to other tumors type.
基金supported by the National Key Research and Development Program of China(2019YFA0110100)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16010000)+6 种基金the National Natural Science Foundation of China(Grant Nos.31900524,31970597,81921006,81625009,91749202,81861168034,91949209,92049304,82125011,81822018,92049116,82071588,82122024,32100937,92149301,and 92168201)the National Key Research and Development Program of China(2018YFC2000100,2020YFA0804000,2017YFA0103300,2017YFA0102800,2018YFA0107200,2019YFA0110900,2020YFA0112200,2020YFA0803401,and 2019YFA0802202)Beijing Natural Science Foundation(Z190019)the Key Research Program of the Chinese Academy of Sciences(KFZD-SW-221)the 14th Five-year Network Security and Informatization Plan of Chinese Academy of Sciences(WX145XQ07-18)K.C.Wong Education Foundation(GJTD-2019-06,GJTD-2019-08)Youth Innovation Promotion Association of CAS(E1CAZW0401)。
文摘Dear Editor, N6-methyladenosine(m6A)is an abundant epitranscriptomic modification that regulates messenger RNA(mRNA)biology.The m6A modification regulates mRNA splicing,transport,stability,and translation through coordinated activities by methyltransferases(writers),binding proteins(readers),and demethylases(erasers)(Huang et al.,2020;Wu et al.,2020).Among m6A regulators,fat mass of obesity-associ-ated protein(FTO),is the first discovered eraser with RNA m6A demethylation activity(Jia et al.,2011).Since then,FTO has been reported to play m6A-dependent roles in a variety of physiological processes including adipogenesis,neuro-genesis and tumorigenesis(Fischer et al.,2009;Li et al.,2017;Huang et al.,2020).Consequently,FTO deficiency in mice leads to dramatic phenotypes,such as decreased fat mass and impaired brain development(Fischer et al.,2009;Li et al.,2017).Similarly,inhibition of FTO reduces tumori-genesis in multiple types of cancer models,while FTO is highly expressed in many cancers(Huang et al.,2020).
