1-Octacosanol(1-Octa)is recognized worldwide as a functional anti-fatigue agent with additional antiinflammatory and energy-boosting benefits.However,it has low bioavailability owing to the poor water solubility and i...1-Octacosanol(1-Octa)is recognized worldwide as a functional anti-fatigue agent with additional antiinflammatory and energy-boosting benefits.However,it has low bioavailability owing to the poor water solubility and instability in the gastrointestinal tract;this limits its use in food,medical,and health care products.To address this,we aimed to prepare a water-soluble octenyl succinate anhydride debranched starch(OSD)-Octa complex and investigate the effects of degree of substitution of OSD and reaction temperature on the drug loading,structure,and water solubility of the complex.The contact angle of free 1-Octa was 110.3°,which decreased to<55°in the complex,and the solubility of 1-Octa in the complex ranged from 0.329±0.008 to 1.532±0.023 mg/mL,which indicated a notable increase in the aqueous solubility of 1-Octa.Furthermore,1-Octa was found to penetrate the amylose helical cavity of OSD and form exohydrophilic and endohydrophobic nanoparticles with OSD through hydrophobic interaction.Finally,OSD-Octa effectively mitigated the degradation of 1-Octa under extreme pH conditions and in the presence of digestive enzymes compared to that of free 1-Octa.These findings suggest that the OSD system is an effective approach to enhancing the solubility and stability of hydrophobic functional factors.展开更多
The maintenance of bioactive proteins is crucial for increasing the quality of infant formula.This study aimed to establish targeted protection through membrane filtration for bacteria removal,whey separation and refi...The maintenance of bioactive proteins is crucial for increasing the quality of infant formula.This study aimed to establish targeted protection through membrane filtration for bacteria removal,whey separation and refilling,low temperature and low pressure spray drying techniques(LTLPSD)to maintain the bioactivity of infant formula.Through optimizing the lactoferrin(Lf)enriched whey(Lf-W)separation,we found that microfiltration(pore sizes of 1.4μm for sterilization and 0.1μm for separating whey)with 0.3M NaCl at 37℃ could achieve 71.53%Lf yield rate in whey.According to commercial processing of infant formula,our control group was processed based on commercial processing techniques,which includes centrifugation and defatting,pasteurization(85℃,15s),ingredient addition,homogenization,sterilization(95℃,15s),evaporation concentration,and LTLPSD.Furthermore,we established the two novel processing routes by applying targeted protection strategy.Experimental Group 1:adding Lf-W before LTLPSD and Experimental Group 2:adding Lf-W before sterilization.The results showed that infant formula produced by Experimental Groups 1 maintained 86.97%of bioactive whey,with and immunoglobulin levels of 1274.40 mg and 2618.07 mg per 100g of total protein,respectively.Furthermore,it had higher enzyme activity,lower glycation level ofα-lactalbumin andβ-lactoglobulin and furosine levels compared to the Experimental Group 2 and Control Group.These results indicated that targeted protection of Lf enriched whey,by avoiding heat treatment as much as possible,significantly reduced the whey protein denaturation,glycation,and keep the immunoactivity of infant formula.展开更多
基金supported by National Natural Science Foundation of China(22276078,32302066)Research Program of State Key Labora-tory of Food Science and Resources,Jiangnan University(No.SKLF-ZZB-202406)+1 种基金China Postdoctoral Science Foundation(2023M731337)Postdoctoral Fellowship Program of CPSF(GZC20230986).
文摘1-Octacosanol(1-Octa)is recognized worldwide as a functional anti-fatigue agent with additional antiinflammatory and energy-boosting benefits.However,it has low bioavailability owing to the poor water solubility and instability in the gastrointestinal tract;this limits its use in food,medical,and health care products.To address this,we aimed to prepare a water-soluble octenyl succinate anhydride debranched starch(OSD)-Octa complex and investigate the effects of degree of substitution of OSD and reaction temperature on the drug loading,structure,and water solubility of the complex.The contact angle of free 1-Octa was 110.3°,which decreased to<55°in the complex,and the solubility of 1-Octa in the complex ranged from 0.329±0.008 to 1.532±0.023 mg/mL,which indicated a notable increase in the aqueous solubility of 1-Octa.Furthermore,1-Octa was found to penetrate the amylose helical cavity of OSD and form exohydrophilic and endohydrophobic nanoparticles with OSD through hydrophobic interaction.Finally,OSD-Octa effectively mitigated the degradation of 1-Octa under extreme pH conditions and in the presence of digestive enzymes compared to that of free 1-Octa.These findings suggest that the OSD system is an effective approach to enhancing the solubility and stability of hydrophobic functional factors.
基金supported by National Key R&D Program of China(2022YFD2101504)Fundamental Research Funds for the Central Universities(JUSRP202416004).
文摘The maintenance of bioactive proteins is crucial for increasing the quality of infant formula.This study aimed to establish targeted protection through membrane filtration for bacteria removal,whey separation and refilling,low temperature and low pressure spray drying techniques(LTLPSD)to maintain the bioactivity of infant formula.Through optimizing the lactoferrin(Lf)enriched whey(Lf-W)separation,we found that microfiltration(pore sizes of 1.4μm for sterilization and 0.1μm for separating whey)with 0.3M NaCl at 37℃ could achieve 71.53%Lf yield rate in whey.According to commercial processing of infant formula,our control group was processed based on commercial processing techniques,which includes centrifugation and defatting,pasteurization(85℃,15s),ingredient addition,homogenization,sterilization(95℃,15s),evaporation concentration,and LTLPSD.Furthermore,we established the two novel processing routes by applying targeted protection strategy.Experimental Group 1:adding Lf-W before LTLPSD and Experimental Group 2:adding Lf-W before sterilization.The results showed that infant formula produced by Experimental Groups 1 maintained 86.97%of bioactive whey,with and immunoglobulin levels of 1274.40 mg and 2618.07 mg per 100g of total protein,respectively.Furthermore,it had higher enzyme activity,lower glycation level ofα-lactalbumin andβ-lactoglobulin and furosine levels compared to the Experimental Group 2 and Control Group.These results indicated that targeted protection of Lf enriched whey,by avoiding heat treatment as much as possible,significantly reduced the whey protein denaturation,glycation,and keep the immunoactivity of infant formula.
