Drought,as major abiotic stress,primarily restricts the production of oat(Avena sativa)around the world.With the aim of elucidating oat leaves’early response to drought stress,this study focused on examining the cont...Drought,as major abiotic stress,primarily restricts the production of oat(Avena sativa)around the world.With the aim of elucidating oat leaves’early response to drought stress,this study focused on examining the contents of malondialdehyde(MDA),soluble sugar(SS),and proline(Pro)and the transcriptome of oat leaves under 0,12,24,36,and 48 h of drought treatment,revealing that the content of MDA increased with drought duration,whereas the contents of SS and Pro reached their maximum at 24 h of treatment,with a total between 0 h of treatment and the other groups.Differentially expressed genes(DEGs)presented significant enrichment in the phenylpropanoid biosynthetic pathway,flavonoid biosynthetic pathway,galactose metabolism,and glutathione metabolism.The biological changes caused by drought stress resulted in the obvious activation of antioxidase genes.The sugar and flavonoid biosynthetic genes were studied based on the detailed temporal patterns of expression of four major metabolic pathways.Additionally,14 candidate hub genes,such as the genes that encoded galactinol synthase,serine/threonineprotein kinase,and 1-aminocyclopropane-1-carboxylate oxidase,were identified by virtue of weighted gene co-expression network analysis.Differential transcription factor(TF)analysis showed that NAM,ATAF1/2,CUC2(NAC),Heat Shock(HS),Trihelix,TEOSINTE BRANCHED1/CYCLOIDEA/PCF(TCP),and other 11 TF families were closely related to drought stress.These findings help us understand the early responses of oat to drought stress from new perspectives and can guide further research.展开更多
The phenylpropane metabolic pathway is one of the most significant metabolic pathways in plants,synthesizing more than 8,000 metabolites,including flavonoids,lignans,lignin,coumarins,and other metabolites through mult...The phenylpropane metabolic pathway is one of the most significant metabolic pathways in plants,synthesizing more than 8,000 metabolites,including flavonoids,lignans,lignin,coumarins,and other metabolites through multiple branching pathways(Zhang and Liu 2015;Gao et al.2024).This pathway is crucial in plant growth,development,and plant-environment interactions(Dong and Lin 2021).展开更多
基金conducted with the funds from the Oat Whole Industry Chain Technology Innovation Teamthe National Key Research and Development Program of China(No.2018YFE0107900)+2 种基金the National Modern Agricultural Industry Technology System(No.CARS-07)the Inner Mongolia Autonomous Region Oat Engineering Laboratory Capacity Building Project(No.BR221023)the Inner Mongolia University Oat Engineering Research Center,the Oat Engineering Laboratory of Inner Mongolia Autonomous Region,and the Inner Mongolia Agricultural University Coarse-grain Industry Collaborative Innovation Center,China.
文摘Drought,as major abiotic stress,primarily restricts the production of oat(Avena sativa)around the world.With the aim of elucidating oat leaves’early response to drought stress,this study focused on examining the contents of malondialdehyde(MDA),soluble sugar(SS),and proline(Pro)and the transcriptome of oat leaves under 0,12,24,36,and 48 h of drought treatment,revealing that the content of MDA increased with drought duration,whereas the contents of SS and Pro reached their maximum at 24 h of treatment,with a total between 0 h of treatment and the other groups.Differentially expressed genes(DEGs)presented significant enrichment in the phenylpropanoid biosynthetic pathway,flavonoid biosynthetic pathway,galactose metabolism,and glutathione metabolism.The biological changes caused by drought stress resulted in the obvious activation of antioxidase genes.The sugar and flavonoid biosynthetic genes were studied based on the detailed temporal patterns of expression of four major metabolic pathways.Additionally,14 candidate hub genes,such as the genes that encoded galactinol synthase,serine/threonineprotein kinase,and 1-aminocyclopropane-1-carboxylate oxidase,were identified by virtue of weighted gene co-expression network analysis.Differential transcription factor(TF)analysis showed that NAM,ATAF1/2,CUC2(NAC),Heat Shock(HS),Trihelix,TEOSINTE BRANCHED1/CYCLOIDEA/PCF(TCP),and other 11 TF families were closely related to drought stress.These findings help us understand the early responses of oat to drought stress from new perspectives and can guide further research.
基金supported by the National Key Research and Development Program of China(2023YFD1600700 and 2023YFD1600701)。
文摘The phenylpropane metabolic pathway is one of the most significant metabolic pathways in plants,synthesizing more than 8,000 metabolites,including flavonoids,lignans,lignin,coumarins,and other metabolites through multiple branching pathways(Zhang and Liu 2015;Gao et al.2024).This pathway is crucial in plant growth,development,and plant-environment interactions(Dong and Lin 2021).
