In this study,we investigated the effects of collagen peptides(CP)and elastic peptides(EP)on UV-induced photoaging in HaCaT,B16,and ESF cells.We measured hyaluronic acid(HA),melanin,and collagen levels,as well as the ...In this study,we investigated the effects of collagen peptides(CP)and elastic peptides(EP)on UV-induced photoaging in HaCaT,B16,and ESF cells.We measured hyaluronic acid(HA),melanin,and collagen levels,as well as the expression of related genes and proteins,to evaluate their potential and combined effects.Compared with UV-irradiated controls,HA content increased by 87.57%(CP),39.13%(EP),and 97.60%(combined treatment).Both peptides enhanced collagen levels,reduced melanin content,and exhibited synergistic effects.Additionally,CP and EP significantly increased Superoxide Dismutase(SOD)and Glutathione Peroxidase(GSH-Px)activities in HaCaT cells,decreased Malondialdehyde(MDA)levels,upregulated Has2 mRNA,and downregulated Hayl1 mRNA.In B16 cells,they downregulated key melanogenesis-related genes and proteins(MITF,CREB,PKA,TYR,TRP-2).In ESF cells,they upregulated genes involved in collagen metabolism(Tgf-β,Smad3,P4ha2,Adamts2,Bmp1,Lox).The combination index method confirmed their synergistic effects on improving skin photoaging.Our findings suggest that CP and EP can ameliorate skin photoaging by modulating key biomolecules in hydration,melanogenesis,and collagen metabolism,providing a basis for developing antiphotoaging strategies.展开更多
文摘In this study,we investigated the effects of collagen peptides(CP)and elastic peptides(EP)on UV-induced photoaging in HaCaT,B16,and ESF cells.We measured hyaluronic acid(HA),melanin,and collagen levels,as well as the expression of related genes and proteins,to evaluate their potential and combined effects.Compared with UV-irradiated controls,HA content increased by 87.57%(CP),39.13%(EP),and 97.60%(combined treatment).Both peptides enhanced collagen levels,reduced melanin content,and exhibited synergistic effects.Additionally,CP and EP significantly increased Superoxide Dismutase(SOD)and Glutathione Peroxidase(GSH-Px)activities in HaCaT cells,decreased Malondialdehyde(MDA)levels,upregulated Has2 mRNA,and downregulated Hayl1 mRNA.In B16 cells,they downregulated key melanogenesis-related genes and proteins(MITF,CREB,PKA,TYR,TRP-2).In ESF cells,they upregulated genes involved in collagen metabolism(Tgf-β,Smad3,P4ha2,Adamts2,Bmp1,Lox).The combination index method confirmed their synergistic effects on improving skin photoaging.Our findings suggest that CP and EP can ameliorate skin photoaging by modulating key biomolecules in hydration,melanogenesis,and collagen metabolism,providing a basis for developing antiphotoaging strategies.
