Near infrared(NIR)fluorescence imaging guided photodynamic therapy(PDT)is a technique which has been developed in many clinical trials due to its advantage of real-time optical monitoring,specific spatiotemporal selec...Near infrared(NIR)fluorescence imaging guided photodynamic therapy(PDT)is a technique which has been developed in many clinical trials due to its advantage of real-time optical monitoring,specific spatiotemporal selectivity,and minimal invasiveness.For this,photosensitizers with NIR fluorescence emission and high^(1)O_(2)generation quantum yield are highly desirable.Herein,we designed and synthesized a"donor-acceptor"(D-A)structured semiconductor polymer(SP),which was then wrapped with an amphiphilic compound(Pluronic■F127)to prepare water-soluble nanoparticles(F-SP NPs).The obtained F-SP NPs exhibit good water solubility,excellent particle size stability,strong absorbance at deep red region,and strong NIR fluorescent emission characteristics.The maximal mass extinction coe±cient and fluorescence quantum yield of these F-SPs were calculated to be 21.7 L/(g·cm)and 6.5%,respectively.Moreover,the^(1)O_(2)quantum yield of 89%for F-SP NPs has been achieved under 635 nm laser irradiation,which is higher than Methylene Blue,Ce6,and PpIX.The outstanding properties of these F-SP NPs originate from their unique D-A molecular characteristic.This work should help guide the design of novel semiconductor polymer for NIR fluorescent imaging guided PDT applications.展开更多
Near-infrared(NIR)-Ⅱ fluorescence and photoacoustic(PA)dual-model imaging-guided photothermal therapy(PTT)can precisely diagnose and treat tumors and evaluate the therapeutic efficacy in real-time.Herein,we utilized ...Near-infrared(NIR)-Ⅱ fluorescence and photoacoustic(PA)dual-model imaging-guided photothermal therapy(PTT)can precisely diagnose and treat tumors and evaluate the therapeutic efficacy in real-time.Herein,we utilized a donor-π-acceptor(D-π-A)structured hemicyanine dye(named M1)with a large conjugated structure and strong intramolecular charge transfer effect and demonstrated that the aggregation of M1 could significantly enhance its photophysical performance by improving its photostability and photothermal conversion capability as compared with M1 in a single molecular state.Furthermore,we prepared water-dispersible NIR-Ⅱ fluorescent nanoparticles(M1 NPs)by wrapping M1 with DSPE-PEG2000-NH2.The obtained M1 NPs exhibit strong NIR-I absorption and NIR-Ⅱ fluorescence with their maxima at 734 and 1040 nm,respectively,with a fluorescence quantum yield of 2.84%.Moreover,M1 NPs also exhibit excellent biocompatibility,good photostability,and high photothermal conversion efficiency of 77.5%.In vitro and in vivo experiments reveal that M1 NPs can effectively image tumors through NIR-Ⅱ fluorescence and PA signals,inhibit DNA replication,trigger cytoskeleton collapse,and eventually induce tumor cell apoptosis under 808 nm laser irradiation.Based on these outstanding properties,the application of M1 NPs in PA and NIR-Ⅱ fluorescence imaging-guided PTT of tumors is demonstrated.展开更多
基金This work was supported by National Natural Science Foundation of China(Nos.61805287 and 62175262)The Open Fund of the State Key Laboratory of Luminescent Materials and Devices(South China University of Technology,No.2021-skllmd-10)+1 种基金The Open Sharing Fund for Large-scale Instruments and Equipment of Central South University(CSUZC202218),Fundamental Research Funds for the Central South Universities(Nos.2020CX021,2020zzts387,and 2020zzts404)Key R&D plan of Hunan Province(No.2022SK2101).
文摘Near infrared(NIR)fluorescence imaging guided photodynamic therapy(PDT)is a technique which has been developed in many clinical trials due to its advantage of real-time optical monitoring,specific spatiotemporal selectivity,and minimal invasiveness.For this,photosensitizers with NIR fluorescence emission and high^(1)O_(2)generation quantum yield are highly desirable.Herein,we designed and synthesized a"donor-acceptor"(D-A)structured semiconductor polymer(SP),which was then wrapped with an amphiphilic compound(Pluronic■F127)to prepare water-soluble nanoparticles(F-SP NPs).The obtained F-SP NPs exhibit good water solubility,excellent particle size stability,strong absorbance at deep red region,and strong NIR fluorescent emission characteristics.The maximal mass extinction coe±cient and fluorescence quantum yield of these F-SPs were calculated to be 21.7 L/(g·cm)and 6.5%,respectively.Moreover,the^(1)O_(2)quantum yield of 89%for F-SP NPs has been achieved under 635 nm laser irradiation,which is higher than Methylene Blue,Ce6,and PpIX.The outstanding properties of these F-SP NPs originate from their unique D-A molecular characteristic.This work should help guide the design of novel semiconductor polymer for NIR fluorescent imaging guided PDT applications.
基金supported by the National Natural Science Foundation of China(62175262)the Fundamental Research Funds for the Central Universities(2020CX021)the Key R&D plan of Hunan Province(2022SK2101)。
基金supported by National Natural Science Foundation of China(No.62175262)Fundamental Research Funds for the Central South University(No.2020CX021)The Science and Technology Innovation Program of Hunan Province(No.2022RC1201).
文摘Near-infrared(NIR)-Ⅱ fluorescence and photoacoustic(PA)dual-model imaging-guided photothermal therapy(PTT)can precisely diagnose and treat tumors and evaluate the therapeutic efficacy in real-time.Herein,we utilized a donor-π-acceptor(D-π-A)structured hemicyanine dye(named M1)with a large conjugated structure and strong intramolecular charge transfer effect and demonstrated that the aggregation of M1 could significantly enhance its photophysical performance by improving its photostability and photothermal conversion capability as compared with M1 in a single molecular state.Furthermore,we prepared water-dispersible NIR-Ⅱ fluorescent nanoparticles(M1 NPs)by wrapping M1 with DSPE-PEG2000-NH2.The obtained M1 NPs exhibit strong NIR-I absorption and NIR-Ⅱ fluorescence with their maxima at 734 and 1040 nm,respectively,with a fluorescence quantum yield of 2.84%.Moreover,M1 NPs also exhibit excellent biocompatibility,good photostability,and high photothermal conversion efficiency of 77.5%.In vitro and in vivo experiments reveal that M1 NPs can effectively image tumors through NIR-Ⅱ fluorescence and PA signals,inhibit DNA replication,trigger cytoskeleton collapse,and eventually induce tumor cell apoptosis under 808 nm laser irradiation.Based on these outstanding properties,the application of M1 NPs in PA and NIR-Ⅱ fluorescence imaging-guided PTT of tumors is demonstrated.
