Pseudomonas aeruginosa is an opportunistic pathogen widely distributed in the natural environment,which can cause a variety of infections,especially in people with low immunity and high pathogenicity.In recent years,s...Pseudomonas aeruginosa is an opportunistic pathogen widely distributed in the natural environment,which can cause a variety of infections,especially in people with low immunity and high pathogenicity.In recent years,significant progress has been made in the detection technology of Pseudomonas aeruginosa,covering traditional methods,molecular biology techniques,immunological methods and automated detection systems.Traditional methods such as the national standard method and the filter membrane method are easy to operate,but have the problems of long time consuming and limited sensitivity.Molecular biological techniques(such as PCR,gene cloning)and immunological methods(such as ELISA,colloidal gold immunochromatography)have significantly improved the sensitivity and specificity of detection,but they require high equipment and technology,and are expensive.Automated detection systems,such as VITEK 2 Compact and AutoMS 1000 mass spectrometry identification system,are excellent in improving detection efficiency and accuracy,but their high cost and complex operation process limit their wide application.In addition,the resistance of Pseudomonas aeruginosa to bacteriostatic agents further increases the difficulty of detection.In this paper,the development and application of immunological detection technology,molecular biological technology and immunological technology of Pseudomonas aeruginosa were reviewed,and the principles,advantages,disadvantages and research progress of various detection technologies of Pseudomonas aeruginosa were described,and the future development trend was prospected,in order to provide reference for the optimization and development of detection methods of Pseudomonas aeruginosa.展开更多
We report the clinical and laboratory findings and successful management of seven patients with critical coronavirus disease 2019(COVID-19)requiring mechanical ventilation(MV).The patients were diagnosed based on epid...We report the clinical and laboratory findings and successful management of seven patients with critical coronavirus disease 2019(COVID-19)requiring mechanical ventilation(MV).The patients were diagnosed based on epidemiological history,clinical manifestations,and nucleic acid testing.Upon diagnosis with COVID-19 of critical severity,the patients were admitted to the intensive care unit,where they received early noninvasive-invasive sequential ventilation,early prone positioning,and bundle pharmacotherapy regimen,which consists of antiviral,anti-inflammation,immune-enhancing,and complication-prophylaxis medicines.The patients presented fever(n=7,100%),dry cough(n=3,42.9%),weakness(n=2,28.6%),chest tightness(n=1,14.3%),and/or muscle pain(n=1,14.3%).All patients had normal or lower than normal white blood cell count/lymphocyte count,and chest computed tomography scans showed bilateral patchy shadows or ground glass opacity in the lungs.Nucleic acid testing confirmed COVID-19 in all seven patients.The median MV duration and intensive care unit stay were 9.9 days(interquartile range,6.5-14.6 days;range,5-17 days)and 12.9 days(interquartile range,9.7-17.6 days;range,7-19 days),respectively.All seven patients were extubated,weaned off MV,transferred to the common ward,and discharged as of the writing of this report.Thus,we concluded that good outcomes for patients with critical COVID-19 can be achieved with early noninvasive-invasive sequential ventilation and bundle pharmacotherapy.展开更多
基金College Students’Innovation and Entrepreneurship Training Program Project(X202511049398)College Students’Innovation and Entrepreneurship Training Program Project(X202511049201)+1 种基金College Students’Innovation and Entrepreneurship Training Program Project(D202504071303298456)Hainan Vocational University of Science and Technology University-Level Scientific Research Funding Project(HKKY2024-87).
文摘Pseudomonas aeruginosa is an opportunistic pathogen widely distributed in the natural environment,which can cause a variety of infections,especially in people with low immunity and high pathogenicity.In recent years,significant progress has been made in the detection technology of Pseudomonas aeruginosa,covering traditional methods,molecular biology techniques,immunological methods and automated detection systems.Traditional methods such as the national standard method and the filter membrane method are easy to operate,but have the problems of long time consuming and limited sensitivity.Molecular biological techniques(such as PCR,gene cloning)and immunological methods(such as ELISA,colloidal gold immunochromatography)have significantly improved the sensitivity and specificity of detection,but they require high equipment and technology,and are expensive.Automated detection systems,such as VITEK 2 Compact and AutoMS 1000 mass spectrometry identification system,are excellent in improving detection efficiency and accuracy,but their high cost and complex operation process limit their wide application.In addition,the resistance of Pseudomonas aeruginosa to bacteriostatic agents further increases the difficulty of detection.In this paper,the development and application of immunological detection technology,molecular biological technology and immunological technology of Pseudomonas aeruginosa were reviewed,and the principles,advantages,disadvantages and research progress of various detection technologies of Pseudomonas aeruginosa were described,and the future development trend was prospected,in order to provide reference for the optimization and development of detection methods of Pseudomonas aeruginosa.
文摘We report the clinical and laboratory findings and successful management of seven patients with critical coronavirus disease 2019(COVID-19)requiring mechanical ventilation(MV).The patients were diagnosed based on epidemiological history,clinical manifestations,and nucleic acid testing.Upon diagnosis with COVID-19 of critical severity,the patients were admitted to the intensive care unit,where they received early noninvasive-invasive sequential ventilation,early prone positioning,and bundle pharmacotherapy regimen,which consists of antiviral,anti-inflammation,immune-enhancing,and complication-prophylaxis medicines.The patients presented fever(n=7,100%),dry cough(n=3,42.9%),weakness(n=2,28.6%),chest tightness(n=1,14.3%),and/or muscle pain(n=1,14.3%).All patients had normal or lower than normal white blood cell count/lymphocyte count,and chest computed tomography scans showed bilateral patchy shadows or ground glass opacity in the lungs.Nucleic acid testing confirmed COVID-19 in all seven patients.The median MV duration and intensive care unit stay were 9.9 days(interquartile range,6.5-14.6 days;range,5-17 days)and 12.9 days(interquartile range,9.7-17.6 days;range,7-19 days),respectively.All seven patients were extubated,weaned off MV,transferred to the common ward,and discharged as of the writing of this report.Thus,we concluded that good outcomes for patients with critical COVID-19 can be achieved with early noninvasive-invasive sequential ventilation and bundle pharmacotherapy.
