High-purity magnesium(HP Mg)and Mg-1Ca,as representations of Mg-matrix implants produced by purifying and alloying,are employed in biomedical applications primarily because of their bioactivity and degradability.The s...High-purity magnesium(HP Mg)and Mg-1Ca,as representations of Mg-matrix implants produced by purifying and alloying,are employed in biomedical applications primarily because of their bioactivity and degradability.The superiority of both degradation properties,the match between degradation and osteo-genesis in vivo,and biosafety are critical problems that will decide future purifying or alloying to construct Mg-based implants and promote clinical translation.The present study investigated the benefits and limitations of degradation behavior and biosafety of HP Mg and Mg-1Ca according to the electro-chemical experiment,hydrogen evolution test,immersion test,and in vivo bone implantation assay.The results indicated that due to its Mg 2 Ca phase,Mg-1Ca exhibited a considerably higher corrosion current density and hydrogen production than HP Mg in vitro.Furthermore,HP Mg and Mg-1Ca display a favorable match between their degradation and the surrounding osteogenesis,resulting in no significant variation in degradation in vivo during 26 weeks.Additionally,the implantation and degradation of HP Mg and Mg-1Ca do not result in major organ dysfunction or pathological abnormalities.This work is expected to lay the foundation for future clinical translation of Mg and Mg alloy orthopedic implants.展开更多
The impaired differentiation ability of resident cells and disordered immune microenvironment in periodontitis pose a huge challenge for bone regeneration.Herein,we construct a piezoelectric hydrogel to rescue the imp...The impaired differentiation ability of resident cells and disordered immune microenvironment in periodontitis pose a huge challenge for bone regeneration.Herein,we construct a piezoelectric hydrogel to rescue the impaired osteogenic capability and rebuild the regenerative immune microenvironment through bioenergetic activation.Under local mechanical stress,the piezoelectric hydrogel generated piezopotential that initiates osteogenic differentiation of inflammatory periodontal ligament stem cells(PDLSCs)via modulating energy metabolism and promoting adenosine triphosphate(ATP)synthesis.Moreover,it also reshapes an anti-inflammatory and pro-regenerative niche through switching M1 macrophages to the M2 phenotype.The synergy of tilapia gelatin and piezoelectric stimulation enhances in situ regeneration in periodontal inflammatory defects of rats.These findings pave a new pathway for treating periodontitis and other immune-related bone defects through piezoelectric stimulation-enabled energy metabolism modulation and immunomodulation.展开更多
Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroit...Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroitin sulfate)double network hydrogel was constructed in this paper.It was not only multiply bionic in its composition,structure and mechanical strength,but also endowed with the ability to immunomodulate microenvironment and simultaneously induce in situ repair of defected TMJ discs.On the one hand,it inhibited inflammatory effects of inflammasome in macrophages,reduced the extracellular matrix(ECM)-degrading enzymes secreted by chondrocytes,reversed the local inflammatory state,promoted the proliferation of TMJ disc cells and induced fibrochondrogenic differentiation of synovium-derived mesenchymal stem cells(SMSCs).On the other hand,it gave an impetus to repairing a relatively-large(6 mm-sized)defect in mini pigs’TMJ discs in a rapid and high-quality manner,which suggested a promising clinical application.展开更多
基金the Science and Technology Commission of Shanghai Municipality(18DZ2201500,19DZ2203900)Innovative Research Team of High-level Local Universities in Shanghai(SHSMU-ZLCX20212400)Research Disci-pline Fund(KQXJXK2021)from Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,and College of Stomatology,Shanghai Jiao Tong University.
文摘High-purity magnesium(HP Mg)and Mg-1Ca,as representations of Mg-matrix implants produced by purifying and alloying,are employed in biomedical applications primarily because of their bioactivity and degradability.The superiority of both degradation properties,the match between degradation and osteo-genesis in vivo,and biosafety are critical problems that will decide future purifying or alloying to construct Mg-based implants and promote clinical translation.The present study investigated the benefits and limitations of degradation behavior and biosafety of HP Mg and Mg-1Ca according to the electro-chemical experiment,hydrogen evolution test,immersion test,and in vivo bone implantation assay.The results indicated that due to its Mg 2 Ca phase,Mg-1Ca exhibited a considerably higher corrosion current density and hydrogen production than HP Mg in vitro.Furthermore,HP Mg and Mg-1Ca display a favorable match between their degradation and the surrounding osteogenesis,resulting in no significant variation in degradation in vivo during 26 weeks.Additionally,the implantation and degradation of HP Mg and Mg-1Ca do not result in major organ dysfunction or pathological abnormalities.This work is expected to lay the foundation for future clinical translation of Mg and Mg alloy orthopedic implants.
基金grants from the National Natural Science Foundation of China(82271024,82072065,82202333)National Clinical Key Specialty(Z155080000004)+5 种基金Science and Technology Commission of Shanghai Municipality(22DZ2201300)Shanghai Pujiang Programme(23PJD050)Innovative Research Team of High-level Local Universities in Shanghai(SHSMU-ZLCX20212400)Research Discipline fund(KQXJXK2023)from Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,and College of Stomatology,Shanghai Jiao Tong University,the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA16021103)the Fundamental Research Funds for the Central Universities(E2EG6802X2,E2E46801)the National Youth Talent Support Program.
文摘The impaired differentiation ability of resident cells and disordered immune microenvironment in periodontitis pose a huge challenge for bone regeneration.Herein,we construct a piezoelectric hydrogel to rescue the impaired osteogenic capability and rebuild the regenerative immune microenvironment through bioenergetic activation.Under local mechanical stress,the piezoelectric hydrogel generated piezopotential that initiates osteogenic differentiation of inflammatory periodontal ligament stem cells(PDLSCs)via modulating energy metabolism and promoting adenosine triphosphate(ATP)synthesis.Moreover,it also reshapes an anti-inflammatory and pro-regenerative niche through switching M1 macrophages to the M2 phenotype.The synergy of tilapia gelatin and piezoelectric stimulation enhances in situ regeneration in periodontal inflammatory defects of rats.These findings pave a new pathway for treating periodontitis and other immune-related bone defects through piezoelectric stimulation-enabled energy metabolism modulation and immunomodulation.
基金This work was sponsored by grants from the National Nature Science Foundation of China(82072070)the National Key Research and Development Program of China(2018YFC1105202)Science and Technology Commission of Shanghai Municipality(21DZ2291700,18DZ2290300).
文摘Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroitin sulfate)double network hydrogel was constructed in this paper.It was not only multiply bionic in its composition,structure and mechanical strength,but also endowed with the ability to immunomodulate microenvironment and simultaneously induce in situ repair of defected TMJ discs.On the one hand,it inhibited inflammatory effects of inflammasome in macrophages,reduced the extracellular matrix(ECM)-degrading enzymes secreted by chondrocytes,reversed the local inflammatory state,promoted the proliferation of TMJ disc cells and induced fibrochondrogenic differentiation of synovium-derived mesenchymal stem cells(SMSCs).On the other hand,it gave an impetus to repairing a relatively-large(6 mm-sized)defect in mini pigs’TMJ discs in a rapid and high-quality manner,which suggested a promising clinical application.
