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口腔副溶血链球菌黏附蛋白糖基化相关基因产物的亚细胞定位及黏附功能研究 被引量:1
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作者 彭志翔 Hui Wu +1 位作者 Paula Fives-Taylor baiming sun 《口腔生物医学》 2010年第2期61-64,共4页
目的调查口腔副溶血链球菌黏附蛋白Fap1糖基化相关基因产物Gap1、Gap2和Gap3的亚细胞定位,以及相关基因缺陷株的黏附能力改变情况。方法等位置换技术获得口腔副溶血链球菌黏附蛋白糖基化相关基因缺陷株gap1-、gap2-和gap3-,穿梭质粒构... 目的调查口腔副溶血链球菌黏附蛋白Fap1糖基化相关基因产物Gap1、Gap2和Gap3的亚细胞定位,以及相关基因缺陷株的黏附能力改变情况。方法等位置换技术获得口腔副溶血链球菌黏附蛋白糖基化相关基因缺陷株gap1-、gap2-和gap3-,穿梭质粒构建该三种基因的补偿株,Western Blot检测Gap1、Gap2和Gap3在副溶血链球菌内的亚细胞定位;闪烁计数法检测gap1-、gap2-和gap3-对羟基磷灰石的黏附能力。结果 Gap1和Gap2被发现分布于所有亚细胞组分中,但主要集中于胞浆和胞膜内,而Gap3仅分布于胞浆和胞膜;体外黏附实验显示gap1-、gap2-和gap3-对羟基磷灰石的黏附能力显著下降。结论糖基化修饰对副溶血链球菌黏附蛋白Fap1的黏附功能至关重要;Gap1、Gap2和Gap3可能在Fap1糖基化修饰过程中协同作用,该协同作用发生在胞内环境。 展开更多
关键词 副溶血链球菌 糖基化相关基因 亚细胞定位 黏附功能
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Basic requirements of dunk tank settings and verification of the disinfection effect in high-level biosafety laboratories 被引量:2
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作者 Dong Wang baiming sun +6 位作者 Xianfeng Zhang Hailong Tong Xiaoyu Zhang Yunting Bu Weiyu Li Si Chen Donglai Wu 《Biosafety and Health》 2020年第3期120-123,共4页
This study presents the structure and application of dunk tanks in high-level biosafety laboratories.The test methods and sterilization effects of dunk tanks were evaluated by using Bacillus subtilis as indicator.Resu... This study presents the structure and application of dunk tanks in high-level biosafety laboratories.The test methods and sterilization effects of dunk tanks were evaluated by using Bacillus subtilis as indicator.Results showed that sterilization effects of dunk tanks could be achieved by immersing the goods in 0.5%sodium hypochlorite disinfectant solution for 30 min at room temperature.Neutralizer sodium thiosulfate had no effect on the growth of Bacillus subtilis,and thus would not affect the disinfection results of sodium hypochlorite.Furthermore,suggestions for safe operation of dunk tanks are also provided in this study. 展开更多
关键词 Biosafety laboratory Dunk tank DISINFECTION Sodium hypochlorite Sodium thiosulfate Bacillus subtilis NEUTRALIZATION
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