The Klotho.(KL)gene is related to aging.In this study,SKL(secreted KL)and heparin were cross:linked to the acellular small intestinal submucosa(SIS).Based on this,small-diameter tissue engineered blood vessels(TEVs)we...The Klotho.(KL)gene is related to aging.In this study,SKL(secreted KL)and heparin were cross:linked to the acellular small intestinal submucosa(SIS).Based on this,small-diameter tissue engineered blood vessels(TEVs)were constructed.The role of SKL on improving the patency of TEVs through promoting cell adherence was investigated.The SIS membrane was cross linked with heparin and SKL,respectively,to prepare heparin group and SKL group artificial blood vessels.The CCK8 assay showed that SKL treatment promoted endothelial cells proliferation.Western blot showed that SKL upregulated the levels of FAK and RHOA.展开更多
Objective To determine the feasibility and accuracy of high-frequency ultrasound in evaluating the left ventricular(LV)structure and function in normal and pressure overload rats and to examine the changes of the left...Objective To determine the feasibility and accuracy of high-frequency ultrasound in evaluating the left ventricular(LV)structure and function in normal and pressure overload rats and to examine the changes of the left ventricle during its transition from hypertrophy to heart failure.Methods Thirty-eight female rats were randomly assigned to normal(n=10),operated(n=16)and sham-operated(n=12)groups.Parastemal long axis and short axis images were acquired by a 7.5 mHz linear ultrasound probe at 12 weeks and 20 weeks after the operation respectively.Resuits Left ventricular structure and function could be satisfactorily imaged for dimensions and mass.Compared to the sham-operated groups,at 12 weeks after the opuration,the operated rats had increased LV wall thickness and mass(P<0.01)with normal cavity and FS%(P>0.05).At 20 weeks after the operation,the LV wall thickness showed no further progressive change and the LV mass increased greatly with slightly dilatedLV cavity and decreased FS%(P<0.05).Conclusions High-frequency echocardiography provides a useful means to noninvasively evaluate LV dimensions,mass and function in rats.It will have great value for evaluating LV remodeling during the transition from LV hypertrophy to heart failure,as well as the effects of intervening drugs.展开更多
文摘The Klotho.(KL)gene is related to aging.In this study,SKL(secreted KL)and heparin were cross:linked to the acellular small intestinal submucosa(SIS).Based on this,small-diameter tissue engineered blood vessels(TEVs)were constructed.The role of SKL on improving the patency of TEVs through promoting cell adherence was investigated.The SIS membrane was cross linked with heparin and SKL,respectively,to prepare heparin group and SKL group artificial blood vessels.The CCK8 assay showed that SKL treatment promoted endothelial cells proliferation.Western blot showed that SKL upregulated the levels of FAK and RHOA.
文摘Objective To determine the feasibility and accuracy of high-frequency ultrasound in evaluating the left ventricular(LV)structure and function in normal and pressure overload rats and to examine the changes of the left ventricle during its transition from hypertrophy to heart failure.Methods Thirty-eight female rats were randomly assigned to normal(n=10),operated(n=16)and sham-operated(n=12)groups.Parastemal long axis and short axis images were acquired by a 7.5 mHz linear ultrasound probe at 12 weeks and 20 weeks after the operation respectively.Resuits Left ventricular structure and function could be satisfactorily imaged for dimensions and mass.Compared to the sham-operated groups,at 12 weeks after the opuration,the operated rats had increased LV wall thickness and mass(P<0.01)with normal cavity and FS%(P>0.05).At 20 weeks after the operation,the LV wall thickness showed no further progressive change and the LV mass increased greatly with slightly dilatedLV cavity and decreased FS%(P<0.05).Conclusions High-frequency echocardiography provides a useful means to noninvasively evaluate LV dimensions,mass and function in rats.It will have great value for evaluating LV remodeling during the transition from LV hypertrophy to heart failure,as well as the effects of intervening drugs.
