BACKGROUND Propolis and honey are known for their antioxidant,hypoglycemic,and antiproteinuric effects.AIM To explore the effect of propolis,and honey,against D-glucose-induced hyperglycemia,acute kidney injury(AKI),l...BACKGROUND Propolis and honey are known for their antioxidant,hypoglycemic,and antiproteinuric effects.AIM To explore the effect of propolis,and honey,against D-glucose-induced hyperglycemia,acute kidney injury(AKI),liver injury,dyslipidemia,and changes in the oxidants and antioxidants in renal,hepatic,and pancreatic tissues.METHODS The chemical analysis and antioxidant content of propolis and honey and their effect on alpha-amylase and alpha-glucosidase activity were studied.The study included five groups of male rats;four groups(2,3,4 and 5)were treated with Dglucose,and one group was untreated,group 1.In addition to D-glucose,groups 3,4,and 5 were treated with propolis,honey,and their combination,respectively.Blood glucose levels,liver and renal function tests,urine protein and electrolytes,oxidant and antioxidant parameters,and histopathological changes in hepatic,renal,and pancreatic tissues were examined.RESULTS Propolis contains a higher level of total protein and exhibits a higher antioxidant activity.Honey has a higher alpha-amylase and glucosidase inhibitory activity than propolis.D-glucose caused a significant elevation of blood glucose,insulin,homeostasis model assessment,blood urea,creatinine,lipid parameters,liver enzymes,and urine protein levels.It significantly increases malondialdehyde and decreases antioxidant parameters in pancreatic,hepatic,and renal tissues.D-glucose caused histopathological changes in hepatic,renal,and pancreatic tissues;these changes were significantly ameliorated by honey and propolis.CONCLUSION Propolis,honey,or their combination treated hyperglycemia,AKI,proteinuria,liver injury,and dyslipidemia induced by D-glucose,most likely,through their antioxidant activity and alpha-amylase and alpha-glucosidase inhibitory activity.This will pave the way for testing this natural combination in the prevention of diabetic complications,as a complement to basic therapies.展开更多
Objective: To determine the phytochemical characterization and antibacterial activity of Melissa oi cinalis essential oil against bacteria responsible for nosocomial infections.Methods: The phytochemical characterizat...Objective: To determine the phytochemical characterization and antibacterial activity of Melissa oi cinalis essential oil against bacteria responsible for nosocomial infections.Methods: The phytochemical characterization of essential oil was evaluated using gas chromatography-flame ionization detector and gas chromatography-mass spectrometer analysis. Antibacterial activity of the oil was tested against four bacterial strains responsible for nosocomial infections: Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus and Citrobacter koseri using disc dif usion method.Results: Thirty three components were identified representing 89.30% of the total oil composition. The yield of essential oil was 0.4% and the predominant components were citronellal(14.40%), isogeraniol(6.40%), geraniol acetate(10.20%), nerol acetate(5.10%), caryophyllene(8.10%) and β-caryophyllene oxide(11.00%). Antibacterial activity of the oil showed the higher activity against all bacterial strains tested.Conclusions: The essential oil extracted from lemon balm can be used to clean the environment of reanimation polyvalent and anesthesia service.展开更多
Objective: To assess the antioxidant content, antimicrobial and antioxidant activities of various propolis samples. Methods: Seven propolis samples were collected from different locations in Morocco, which are charact...Objective: To assess the antioxidant content, antimicrobial and antioxidant activities of various propolis samples. Methods: Seven propolis samples were collected from different locations in Morocco, which are characterized by different plant predominant vegetations. The resin, wax and balsam of hydroalcoholic extract of propolis content were identified, and the antioxidant content was analyzed with the use of HPLC and colorimetric methods. The antioxidant activity was assessed by DPPH, ABTS.^+ and ferric reducing power assays. The antimicrobial activity was assessed against bacterial species, including methicillin resistant Staphylococcus aureus and Candida albicans, and expressed as the minimal inhibitory concentration. Results: The propolis samples showed significant variations in the chemical composition and in the antioxidant or antimicrobial activities even when the samples were collected from the same location. Propolis with high resin and low wax content had high level of antioxidant compounds, and strong antioxidant and antimicrobial activities. Gram-positive bacteria, especially, methicillin resistant Staphylococcus aureus were more sensitive to all propolis samples than Gram-negative bacteria and Candida albicans. Conclusions: The chemical composition and the antioxidant and antimicrobial activities of various propolis samples are different and rely on the geographic and plant origin of propolis collection. Propolis samples with low wax and high resin content might be more suitable to be used in future preclinical or clinical investigations.展开更多
Objective: To study the antioxidant properties of Capparis spinosa(C.spinosa) honey and propolis and the effect of combined honey and propolis administration on urine volume and electrolytes in rats.Methods: C.spinosa...Objective: To study the antioxidant properties of Capparis spinosa(C.spinosa) honey and propolis and the effect of combined honey and propolis administration on urine volume and electrolytes in rats.Methods: C.spinosa honey [1 000 mg/kg body weight(b.wt)], propolis(100 mg/kg b.wt), honey/propolis mixture(C.spinosa honey 1 000 mg/kg b.wt/propolis extract 100 mg/kg b.wt), distilled water(1 mL/kg b.wt) and furosemide(10 mg/kg b.wt) were orally administered to fi ve groups of rats for 21 d.Urine volume, blood and urine sodium,potassium and chloride were measured.The antioxidant activity of propolis and honey was assessed and their total phenols and flavonoids were determined.Results: Propolis and C.spinosa honey contain polyphenols including flavonoids and propolis demonstrated higher antioxidant activities than honey.Honey significantly increased urine volume and urine electrolyte excretion.Propolis had no significant effect on urine volume, but co-administration of propolis and honey caused significant diuresis.No major changes were observed in plasma electrolytes with the use of honey, propolis or their combination.Conclusions: Honey and propolis have antioxidant activity and contain polyphenols including flavonoids that are more pronounced in propolis.Honey has a significant diuretic activity alone or in combination with propolis.This is the first study comparing the diuretic effect of co-administration of propolis and C.spinosa honey with furosemide.展开更多
Objective: To investigate the diuretic, hypotensive and renal effect of Opuntia ficusindica in two different species in oral and intravenous administration.Methods: Diuretic activity was evaluated in rats with the pla...Objective: To investigate the diuretic, hypotensive and renal effect of Opuntia ficusindica in two different species in oral and intravenous administration.Methods: Diuretic activity was evaluated in rats with the plant cladode gel and aqueous extract administrated orally, and was evaluated in rabbits with plant extract administered intravenously. Single and repeated doses of cladode gel or aqueous extract of cladode were tested. Urine volume and blood and urine creatinine, sodium and potassium were measured, and creatinine clearance was calculated. The hypotensive effect of lyophilized extract of cladode was evaluated in rabbits. Two polyethylene PE50 catheters were used:one in the jugular vein for the infusion of the plant extract and the other in the carotid for the evaluation of the arterial pressure.Results: The cladode gel or aqueous extract increased urine volume, creatinine clearance and urinary excretion of sodium and potassium without significant effect on serum creatinine or blood urea. Furosemide, gel and aqueous extract of cladode insignificantly lowered plasma potassium in rats. Intravenous administration of the lyophilized extract caused a significant decrease in mean arterial pressure in rabbits with a significant increase in urine volume and urine sodium and potassium; the effect was dose dependent.Intravenous administration of lyophilized extract did not affect plasma sodium or potassium.Conclusions: Gel and aqueous extract of Opuntia ficus-indica cladode have a significant diuretic effect on rats, and the lyophilized extract has a diuretic and hypotensive effect on normotensive rabbits without deterioration in renal function test. Additional studies on active ingredients are essential to pave the way for clinical studies on diuretic and hypotensive effect of the plant.展开更多
Objective:To investigate the diuretic and renal effects of Silybum marianum L.and Cistus ladaniferus L.in normal rats.Methods:Four groups of rats were used in each experiment.The first group received water,the second ...Objective:To investigate the diuretic and renal effects of Silybum marianum L.and Cistus ladaniferus L.in normal rats.Methods:Four groups of rats were used in each experiment.The first group received water,the second group received Cistus ladaniferus L.extract(100 mg/kg b.wt),the third group received Silybum marianum L.extract(100 mg/kg b.wt),and the fourth group received furosemide(10 mg/kg b.wt).Variables including urine volume,plasma and urine sodium,potassium and creatinine,and creatinine clearance were measured.Two experiments were conducted.A single dose of each intervention was used and the variables were measured during 24 h,and the interventions were given daily for a total of 8 d and the variables were measured during various intervals.Results:The single dose of each plant extract increased urine volume at all-time intervals and increased urine sodium and potassium excretion without affecting plasma sodium and potassium(P<0.05).On the day 8 after daily administration,the plant extracts induced a significant diuresis and natriuresis without affecting serum electrolytes(P<0.05),while furosemide caused hypokalemia.Both plant extracts significantly increased creatinine clearance(P<0.05).Conclusions:Silybum marianum L.and Cistus ladaniferus L Increase creatinine clearance and have a significant diuretic effect without affecting serum electrolytes.Silybum marianum L.is more potent than furosemide or Cistus ladaniferus L.展开更多
Currently,several studies have demonstrated the benefits of medicinal plants in managing type 2 diabetes.In this work,we evaluated the beneficial effects of the polyphenolic extract(PESB)from Salvia blancoana subsp.me...Currently,several studies have demonstrated the benefits of medicinal plants in managing type 2 diabetes.In this work,we evaluated the beneficial effects of the polyphenolic extract(PESB)from Salvia blancoana subsp.mesatlan-tica in the management of hypercaloric-feeding and small-dose alloxan-brought type 2 diabetes in rats.We analyzed the chemical constituents of the extract,including flavones and flavonols content,to understand its biological action.The antioxidant activities were evaluated by total antioxidant action,scavenging effect of the free radical DPPH,and reducing power.The obtained results showed that the value of TFC was estimated at 31.90±0.34 mgEQ/g in the PESB extract.The total antioxidant capacity was estimated at 593.51±4.09 mg(EAA)/g,the value of DPPH IC50 was 7.3±0.00μg/mL,and the value of EC50 of reducing power was estimated at 6.43±0.01μg/mL.In total,14 phenolic compounds were identified and the naringin was the most dominant(63.19%)while the vanillin was the less recorded(0.10%).Serum glucose decreased significantly(p<0.05)in rats given PESB(100 mg/kg)after four weeks.Glibenclamide(GLB)and PESB reduced HbA1c and increased plasma insulin in diabetic rats,restoring HOMA-βand HOMA-IR levels to near-normal.Additionally,diabetic rats treated with GLB or PESB showed statistically equivalent results to those of non-diabetic rats regarding hepatic enzymes,renal and lipid markers,as well as cardiovascular indices.The weight loss was significantly lower in diabetic rats receiving a dose of PESB(100 mg/kg),and GLB compared to corresponding untreated diabetic rats(p<0.01).PESB and GLB showed a prominent protective function in the pancreas,liver,and kidney tissues.This investigation demonstrates the capacity of extracts from leaves of S.blancoana subsp.mesatlantica to manage diabetes mellitus due to their richness in a wide range of bioactive compounds.Therefore,more investigations are required to estimate the safety of the plant use.展开更多
文摘BACKGROUND Propolis and honey are known for their antioxidant,hypoglycemic,and antiproteinuric effects.AIM To explore the effect of propolis,and honey,against D-glucose-induced hyperglycemia,acute kidney injury(AKI),liver injury,dyslipidemia,and changes in the oxidants and antioxidants in renal,hepatic,and pancreatic tissues.METHODS The chemical analysis and antioxidant content of propolis and honey and their effect on alpha-amylase and alpha-glucosidase activity were studied.The study included five groups of male rats;four groups(2,3,4 and 5)were treated with Dglucose,and one group was untreated,group 1.In addition to D-glucose,groups 3,4,and 5 were treated with propolis,honey,and their combination,respectively.Blood glucose levels,liver and renal function tests,urine protein and electrolytes,oxidant and antioxidant parameters,and histopathological changes in hepatic,renal,and pancreatic tissues were examined.RESULTS Propolis contains a higher level of total protein and exhibits a higher antioxidant activity.Honey has a higher alpha-amylase and glucosidase inhibitory activity than propolis.D-glucose caused a significant elevation of blood glucose,insulin,homeostasis model assessment,blood urea,creatinine,lipid parameters,liver enzymes,and urine protein levels.It significantly increases malondialdehyde and decreases antioxidant parameters in pancreatic,hepatic,and renal tissues.D-glucose caused histopathological changes in hepatic,renal,and pancreatic tissues;these changes were significantly ameliorated by honey and propolis.CONCLUSION Propolis,honey,or their combination treated hyperglycemia,AKI,proteinuria,liver injury,and dyslipidemia induced by D-glucose,most likely,through their antioxidant activity and alpha-amylase and alpha-glucosidase inhibitory activity.This will pave the way for testing this natural combination in the prevention of diabetic complications,as a complement to basic therapies.
文摘Objective: To determine the phytochemical characterization and antibacterial activity of Melissa oi cinalis essential oil against bacteria responsible for nosocomial infections.Methods: The phytochemical characterization of essential oil was evaluated using gas chromatography-flame ionization detector and gas chromatography-mass spectrometer analysis. Antibacterial activity of the oil was tested against four bacterial strains responsible for nosocomial infections: Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus and Citrobacter koseri using disc dif usion method.Results: Thirty three components were identified representing 89.30% of the total oil composition. The yield of essential oil was 0.4% and the predominant components were citronellal(14.40%), isogeraniol(6.40%), geraniol acetate(10.20%), nerol acetate(5.10%), caryophyllene(8.10%) and β-caryophyllene oxide(11.00%). Antibacterial activity of the oil showed the higher activity against all bacterial strains tested.Conclusions: The essential oil extracted from lemon balm can be used to clean the environment of reanimation polyvalent and anesthesia service.
文摘Objective: To assess the antioxidant content, antimicrobial and antioxidant activities of various propolis samples. Methods: Seven propolis samples were collected from different locations in Morocco, which are characterized by different plant predominant vegetations. The resin, wax and balsam of hydroalcoholic extract of propolis content were identified, and the antioxidant content was analyzed with the use of HPLC and colorimetric methods. The antioxidant activity was assessed by DPPH, ABTS.^+ and ferric reducing power assays. The antimicrobial activity was assessed against bacterial species, including methicillin resistant Staphylococcus aureus and Candida albicans, and expressed as the minimal inhibitory concentration. Results: The propolis samples showed significant variations in the chemical composition and in the antioxidant or antimicrobial activities even when the samples were collected from the same location. Propolis with high resin and low wax content had high level of antioxidant compounds, and strong antioxidant and antimicrobial activities. Gram-positive bacteria, especially, methicillin resistant Staphylococcus aureus were more sensitive to all propolis samples than Gram-negative bacteria and Candida albicans. Conclusions: The chemical composition and the antioxidant and antimicrobial activities of various propolis samples are different and rely on the geographic and plant origin of propolis collection. Propolis samples with low wax and high resin content might be more suitable to be used in future preclinical or clinical investigations.
文摘Objective: To study the antioxidant properties of Capparis spinosa(C.spinosa) honey and propolis and the effect of combined honey and propolis administration on urine volume and electrolytes in rats.Methods: C.spinosa honey [1 000 mg/kg body weight(b.wt)], propolis(100 mg/kg b.wt), honey/propolis mixture(C.spinosa honey 1 000 mg/kg b.wt/propolis extract 100 mg/kg b.wt), distilled water(1 mL/kg b.wt) and furosemide(10 mg/kg b.wt) were orally administered to fi ve groups of rats for 21 d.Urine volume, blood and urine sodium,potassium and chloride were measured.The antioxidant activity of propolis and honey was assessed and their total phenols and flavonoids were determined.Results: Propolis and C.spinosa honey contain polyphenols including flavonoids and propolis demonstrated higher antioxidant activities than honey.Honey significantly increased urine volume and urine electrolyte excretion.Propolis had no significant effect on urine volume, but co-administration of propolis and honey caused significant diuresis.No major changes were observed in plasma electrolytes with the use of honey, propolis or their combination.Conclusions: Honey and propolis have antioxidant activity and contain polyphenols including flavonoids that are more pronounced in propolis.Honey has a significant diuretic activity alone or in combination with propolis.This is the first study comparing the diuretic effect of co-administration of propolis and C.spinosa honey with furosemide.
文摘Objective: To investigate the diuretic, hypotensive and renal effect of Opuntia ficusindica in two different species in oral and intravenous administration.Methods: Diuretic activity was evaluated in rats with the plant cladode gel and aqueous extract administrated orally, and was evaluated in rabbits with plant extract administered intravenously. Single and repeated doses of cladode gel or aqueous extract of cladode were tested. Urine volume and blood and urine creatinine, sodium and potassium were measured, and creatinine clearance was calculated. The hypotensive effect of lyophilized extract of cladode was evaluated in rabbits. Two polyethylene PE50 catheters were used:one in the jugular vein for the infusion of the plant extract and the other in the carotid for the evaluation of the arterial pressure.Results: The cladode gel or aqueous extract increased urine volume, creatinine clearance and urinary excretion of sodium and potassium without significant effect on serum creatinine or blood urea. Furosemide, gel and aqueous extract of cladode insignificantly lowered plasma potassium in rats. Intravenous administration of the lyophilized extract caused a significant decrease in mean arterial pressure in rabbits with a significant increase in urine volume and urine sodium and potassium; the effect was dose dependent.Intravenous administration of lyophilized extract did not affect plasma sodium or potassium.Conclusions: Gel and aqueous extract of Opuntia ficus-indica cladode have a significant diuretic effect on rats, and the lyophilized extract has a diuretic and hypotensive effect on normotensive rabbits without deterioration in renal function test. Additional studies on active ingredients are essential to pave the way for clinical studies on diuretic and hypotensive effect of the plant.
文摘Objective:To investigate the diuretic and renal effects of Silybum marianum L.and Cistus ladaniferus L.in normal rats.Methods:Four groups of rats were used in each experiment.The first group received water,the second group received Cistus ladaniferus L.extract(100 mg/kg b.wt),the third group received Silybum marianum L.extract(100 mg/kg b.wt),and the fourth group received furosemide(10 mg/kg b.wt).Variables including urine volume,plasma and urine sodium,potassium and creatinine,and creatinine clearance were measured.Two experiments were conducted.A single dose of each intervention was used and the variables were measured during 24 h,and the interventions were given daily for a total of 8 d and the variables were measured during various intervals.Results:The single dose of each plant extract increased urine volume at all-time intervals and increased urine sodium and potassium excretion without affecting plasma sodium and potassium(P<0.05).On the day 8 after daily administration,the plant extracts induced a significant diuresis and natriuresis without affecting serum electrolytes(P<0.05),while furosemide caused hypokalemia.Both plant extracts significantly increased creatinine clearance(P<0.05).Conclusions:Silybum marianum L.and Cistus ladaniferus L Increase creatinine clearance and have a significant diuretic effect without affecting serum electrolytes.Silybum marianum L.is more potent than furosemide or Cistus ladaniferus L.
基金financially supported by the Researchers Supporting Project(RSP2024R457).King Saud University,Riyadh,Saudi Arabia.
文摘Currently,several studies have demonstrated the benefits of medicinal plants in managing type 2 diabetes.In this work,we evaluated the beneficial effects of the polyphenolic extract(PESB)from Salvia blancoana subsp.mesatlan-tica in the management of hypercaloric-feeding and small-dose alloxan-brought type 2 diabetes in rats.We analyzed the chemical constituents of the extract,including flavones and flavonols content,to understand its biological action.The antioxidant activities were evaluated by total antioxidant action,scavenging effect of the free radical DPPH,and reducing power.The obtained results showed that the value of TFC was estimated at 31.90±0.34 mgEQ/g in the PESB extract.The total antioxidant capacity was estimated at 593.51±4.09 mg(EAA)/g,the value of DPPH IC50 was 7.3±0.00μg/mL,and the value of EC50 of reducing power was estimated at 6.43±0.01μg/mL.In total,14 phenolic compounds were identified and the naringin was the most dominant(63.19%)while the vanillin was the less recorded(0.10%).Serum glucose decreased significantly(p<0.05)in rats given PESB(100 mg/kg)after four weeks.Glibenclamide(GLB)and PESB reduced HbA1c and increased plasma insulin in diabetic rats,restoring HOMA-βand HOMA-IR levels to near-normal.Additionally,diabetic rats treated with GLB or PESB showed statistically equivalent results to those of non-diabetic rats regarding hepatic enzymes,renal and lipid markers,as well as cardiovascular indices.The weight loss was significantly lower in diabetic rats receiving a dose of PESB(100 mg/kg),and GLB compared to corresponding untreated diabetic rats(p<0.01).PESB and GLB showed a prominent protective function in the pancreas,liver,and kidney tissues.This investigation demonstrates the capacity of extracts from leaves of S.blancoana subsp.mesatlantica to manage diabetes mellitus due to their richness in a wide range of bioactive compounds.Therefore,more investigations are required to estimate the safety of the plant use.
