Brucea javanica oil emulsion(BJOE)has been used to treat tumor in China for more than 40 years.However,its components and effectiveness in the treatment of acute lymphocytic leukemia(ALL)and its mechanism of anti-canc...Brucea javanica oil emulsion(BJOE)has been used to treat tumor in China for more than 40 years.However,its components and effectiveness in the treatment of acute lymphocytic leukemia(ALL)and its mechanism of anti-cancer activity remain unknown.In the current study,high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)was used to analyze the components of BJOE.Then,the anti-leukemia effects of BJOE were examined both in vitro and in vivo using ALL Jurkat cells and the p388 mouse leukemia transplant model,respectively.The primary ALL leukemia cells were also used to confirm the antileukemia effects of BJOE.The apoptotic-related results indicated that BJOE induced apoptosis in Jurkat cells and were suggestive of intrinsic apoptotic induction.Moreover,BJOE inhibited Akt(protein kinase B)activation and upregulated its downstream targets p53 and Fox O1(forkhead box gene,group O-1)to initiate apoptosis.The activation of GSK3βwas also involved.Our findings demonstrate that BJOE has anti-leukemia effects on ALL cells and can induce apoptosis in Jurkat cells through the phosphoinositide3-kinase(PI3 K)/Akt signaling pathway.展开更多
基金supported by the Young Scientific and Technological Talents Seeding Program of Liaoning Education Office(No.2019LQN10)Shenyang Young and Middle-Aged Science and Technology Innovation Program(No.RC180308)。
文摘Brucea javanica oil emulsion(BJOE)has been used to treat tumor in China for more than 40 years.However,its components and effectiveness in the treatment of acute lymphocytic leukemia(ALL)and its mechanism of anti-cancer activity remain unknown.In the current study,high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)was used to analyze the components of BJOE.Then,the anti-leukemia effects of BJOE were examined both in vitro and in vivo using ALL Jurkat cells and the p388 mouse leukemia transplant model,respectively.The primary ALL leukemia cells were also used to confirm the antileukemia effects of BJOE.The apoptotic-related results indicated that BJOE induced apoptosis in Jurkat cells and were suggestive of intrinsic apoptotic induction.Moreover,BJOE inhibited Akt(protein kinase B)activation and upregulated its downstream targets p53 and Fox O1(forkhead box gene,group O-1)to initiate apoptosis.The activation of GSK3βwas also involved.Our findings demonstrate that BJOE has anti-leukemia effects on ALL cells and can induce apoptosis in Jurkat cells through the phosphoinositide3-kinase(PI3 K)/Akt signaling pathway.
