The gene of Helicobacter pylori can encode three to four type IV secretory systems,of which a new gene region has been found in the H.pylori plasticity region.The coding products of this region can form a new T4SS nam...The gene of Helicobacter pylori can encode three to four type IV secretory systems,of which a new gene region has been found in the H.pylori plasticity region.The coding products of this region can form a new T4SS named tfs3,but its function is unclear.This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H.pylori clinical strain SBK on GES-1 cells.We observed changes in cell morphology after VirD4 treatment.Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB.VirD4 can also inhibited proliferation,and induced migration of cells.Moreover,VirD4 caused apoptosis in GES-1 cells in caspase and ERK1/2/Ras dependent signaling events.Our study laid a foundation for further research on the biological function of VirD4 and the detection and treatment of H.pylori-related diseases.展开更多
Exosomes,especially from mesenchymal stem cells,have attracted extensive attention in regeneration medicine.Mesenchymal stem cells derived exosomes(MSCs-exosomes)have shown anti-inflammatory,anti-oxidant,antiapoptosis...Exosomes,especially from mesenchymal stem cells,have attracted extensive attention in regeneration medicine.Mesenchymal stem cells derived exosomes(MSCs-exosomes)have shown anti-inflammatory,anti-oxidant,antiapoptosis and tissue regeneration effects in a variety of tissue injury repair models.MSCs-exosomes hold many excellent properties such as low immunogenicity,biocompatibility,and targeting capability.With the in-depth study on the generation and function of exosomes,MSCs-exosomes are considered to be the bright stars in the field of regenerative medicine.However,there are still many obstacles to overcome in terms of exosomes isolation,clinical trials and safety evaluation.In this article,what we should focus on about MSCs-exosomes in regeneration medicine will be discussed.展开更多
基金This work was supported by the Natural Science Foundation of Jiangsu Province(H2018072)Taizhou Social Development Project(TS201623).
文摘The gene of Helicobacter pylori can encode three to four type IV secretory systems,of which a new gene region has been found in the H.pylori plasticity region.The coding products of this region can form a new T4SS named tfs3,but its function is unclear.This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H.pylori clinical strain SBK on GES-1 cells.We observed changes in cell morphology after VirD4 treatment.Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB.VirD4 can also inhibited proliferation,and induced migration of cells.Moreover,VirD4 caused apoptosis in GES-1 cells in caspase and ERK1/2/Ras dependent signaling events.Our study laid a foundation for further research on the biological function of VirD4 and the detection and treatment of H.pylori-related diseases.
基金This work was supported by the National Natural Science Foundation of China(81871496,81971757)Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and Transformation Application(ss2018003)Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD III).
文摘Exosomes,especially from mesenchymal stem cells,have attracted extensive attention in regeneration medicine.Mesenchymal stem cells derived exosomes(MSCs-exosomes)have shown anti-inflammatory,anti-oxidant,antiapoptosis and tissue regeneration effects in a variety of tissue injury repair models.MSCs-exosomes hold many excellent properties such as low immunogenicity,biocompatibility,and targeting capability.With the in-depth study on the generation and function of exosomes,MSCs-exosomes are considered to be the bright stars in the field of regenerative medicine.However,there are still many obstacles to overcome in terms of exosomes isolation,clinical trials and safety evaluation.In this article,what we should focus on about MSCs-exosomes in regeneration medicine will be discussed.
