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藏药经典名方索罗西汤颗粒通过Nrf2/HO-1及PI3K/AKT/mTOR双通路调节氧化应激缓解大鼠肺纤维化进程
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作者 杜金洋 白玛拉宗 +3 位作者 德吉白珍 巴桑德吉 边巴次仁 叶本贵 《四川大学学报(医学版)》 北大核心 2026年第2期482-490,共9页
目的考察藏药经典名方索罗西汤颗粒(Srolo Bzhtang,SBT)通过核因子E2相关因子2(nuclear factor erythroid-2-related actor 2,Nrf2)/血红素氧合酶1(heme oxygenase-1,HO-1)及磷脂酰肌醇3-激酶(phosphatidylinositide 3-kinases,PI3K)/... 目的考察藏药经典名方索罗西汤颗粒(Srolo Bzhtang,SBT)通过核因子E2相关因子2(nuclear factor erythroid-2-related actor 2,Nrf2)/血红素氧合酶1(heme oxygenase-1,HO-1)及磷脂酰肌醇3-激酶(phosphatidylinositide 3-kinases,PI3K)/蛋白激酶B(protein kinase B,AKT)/雷帕霉素靶点(mammalian target of rapamycin,mTOR)通路改善氧化应激,最终缓解大鼠肺纤维化进程的作用机制。方法将72只SD大鼠随机分为12只假手术组(Sham组,注等量生理盐水)和60只造模组(采用博来霉素气管滴注法建立大鼠肺纤维化模型)。造模24 h后,造模组随机分为模型组(Model)、阳性药组(pirfenidone,150 mg/kg)及索罗西汤低、中、高剂量组(SBT-L组0.5 g/kg、SBT-M组1.5 g/kg、SBT-H组4.5 g/kg),每组12只,每日1次灌胃给药共21 d。Sham组与Model组灌胃等量生理盐水。HE及Masson染色观察各组大鼠肺纤维化病理情况,ELISA测定血清中炎症因子(TNF-α、IL-8)及基质金属蛋白酶(MMP-2、MMP-9)水平;并检测各组大鼠血清中丙二醛(malondialdehyde,MDA)及超氧化物歧化酶(superoxide dismutase,SOD)活力;蛋白质免疫印迹技术测定肺组织中Nrf2/HO-1与PI3K/AKT/mTOR信号通路蛋白的表达水平。结果HE及Masson染色见Model组较Sham组大鼠肺纤维化程度加重,各给药组可不同程度逆转此进程。SBT能够抑制炎症因子TNF-α、IL-8水平(P均<0.05),同时与Model组相比,基质金属蛋白酶MMP-2、MMP-9水平降低(P均<0.05)。病理切片结果表明SBT能一定程度上改善肺纤维化大鼠的肺组织损伤。与Model组相比,低、中、高剂量SBT组的MDA水平均下降(P均<0.05),SOD酶活力呈现促进趋势。蛋白质免疫印迹结果提示,与Model组相比,低、中、高剂量SBT组能够激活Nrf2及HO-1的蛋白表达(P均<0.05),下调p-PI3K/PI3K、p-AKT/AKT、p-mTOR/mTOR的表达水平(P均<0.05)。结论SBT通过影响Nrf2/HO-1与PI3K/AKT/mTOR信号通路,抑制氧化应激,从而延缓大鼠肺纤维化进程。 展开更多
关键词 藏药经典名方 索罗西汤颗粒 肺纤维化 氧化应激 机制通路
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Prevention and Control and Methods of Agricultural Diseases and Insect Pests in Tibet
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作者 baimalazong 《外文科技期刊数据库(文摘版)自然科学》 2022年第3期132-135,共4页
As a large agricultural country,China's agricultural development level not only affects the quality of life of the residents,but also affects China's economic development level.However,the occurrence of plant ... As a large agricultural country,China's agricultural development level not only affects the quality of life of the residents,but also affects China's economic development level.However,the occurrence of plant diseases and insect pests every year will bring certain disasters and great economic losses to agriculture.Therefore,it is particularly important to do a good job in the prevention and control of agricultural plant diseases and insect pests.However,as an extremely cold region,Tibet's economic development is relatively backward.Although relevant measures have been taken to control plant diseases and insect pests,the overall effect is still not satisfactory.Therefore,in order to avoid the occurrence of large-scale diseases and insect pests in Tibet,we should do a good job in the reasonable control of diseases and insect pests,and adopt relevant measures to reduce the occurrence rate of diseases and insect pests,and ensure that the economic losses caused by diseases and insect pests to farmers are minimized,so as to effectively improve the planting efficiency of farmers'crops. 展开更多
关键词 TIBET agricultural development pest control WAY
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