Medium-and long-chain structured lipids(MLSLs)rich in docosahexaenoic acid(DHA)and eicosapentaenoic acid(EPA)were synthesized by acidolysis of fish oil(Omax 1812,a mixture of sardine and anchovy oils)with caprylic aci...Medium-and long-chain structured lipids(MLSLs)rich in docosahexaenoic acid(DHA)and eicosapentaenoic acid(EPA)were synthesized by acidolysis of fish oil(Omax 1812,a mixture of sardine and anchovy oils)with caprylic acid(CA)using a lipase bio-imprinted with fatty acids as a catalyst.A bio-imprinted lipase for the acidolysis reaction was first prepared under optimal conditions,and its activity was 1.43 times higher than that of the non-bioimprinted.The bio-imprinted lipase was used as a catalyst to produce such MLSLs,and the reaction conditions were optimized.Under the optimal conditions,equilibrium was achieved in 8 h,and the total and the sn-1,3 CA contents in the obtained product were 28.72%and 42.26%.Additionally,the total and the sn-2 DHA contents were 14.23%and 21.10%,respectively,and the total and the sn-2 EPA contents were 16.43%and 10.14%,respectively.These obtained MLSLs,rich in sn-1,3 CA,and sn-2 DHA,EPA can deliver significant health benefits.展开更多
基金supported by the National Natural Science Foundation of China(31601433).
文摘Medium-and long-chain structured lipids(MLSLs)rich in docosahexaenoic acid(DHA)and eicosapentaenoic acid(EPA)were synthesized by acidolysis of fish oil(Omax 1812,a mixture of sardine and anchovy oils)with caprylic acid(CA)using a lipase bio-imprinted with fatty acids as a catalyst.A bio-imprinted lipase for the acidolysis reaction was first prepared under optimal conditions,and its activity was 1.43 times higher than that of the non-bioimprinted.The bio-imprinted lipase was used as a catalyst to produce such MLSLs,and the reaction conditions were optimized.Under the optimal conditions,equilibrium was achieved in 8 h,and the total and the sn-1,3 CA contents in the obtained product were 28.72%and 42.26%.Additionally,the total and the sn-2 DHA contents were 14.23%and 21.10%,respectively,and the total and the sn-2 EPA contents were 16.43%and 10.14%,respectively.These obtained MLSLs,rich in sn-1,3 CA,and sn-2 DHA,EPA can deliver significant health benefits.
