This paper builds on exploring the applications of biomediated pathways to solve geotechnical challenges.First,the state of the art of biological remediation strategies including microbial remediation and phytoremedia...This paper builds on exploring the applications of biomediated pathways to solve geotechnical challenges.First,the state of the art of biological remediation strategies including microbial remediation and phytoremediation have been introduced and critically reviewed in the context of decontaminating the soils.Next,biopolymerisation,biomineralisation and bioneutralisation processes have been depicted with a special emphasis on the applications including but not limited to soil stabilisation,soil erosion prevention,anti-desertification and pH neutralisation.Each of these methods have their own limitations and bottlenecks while scaling up,and these challenges have been summarised and some possible paths to overcome the challenges have also been discussed.The state of the art of electromagnetic(EM)monitoring methods to capture the effects of biomediation on spatio-temporal soil properties are then highlighted as a non-invasive and rapid pathway to track the progress of biomediated soil processes.Finally,each of the technologies discussed have been evaluated for their maturity level using the principles of technology readiness level(TRL).A majority of the technologies amounting to around 77%are still in the TRL 4e7,i.e.in the valley of death.It is thus evident that development of these technologies needs to be supported with appropriate funding for improving their maturity to a level of industrial deployment.展开更多
The renewable hydrogen generation through ethanol steam reforming is one of the anticipated areas for sustainable hydrogen generation. To elucidate the role of Ni and Co with ZSM-5 support, catalysts were prepared by ...The renewable hydrogen generation through ethanol steam reforming is one of the anticipated areas for sustainable hydrogen generation. To elucidate the role of Ni and Co with ZSM-5 support, catalysts were prepared by wet impregnation method and ethanol steam reforming(ESR) was performed. The catalysts were characterized by HR-XRD, ATR–FTIR, HR-SEM, TEM with SAED, EDAX, surface area analyzer and TPR. It had shown complete ethanol conversion at 773 K, but the selectivity in hydrogen generation was found higher for 10% Ni/ZSM-5 catalyst as compared to 10% Co/ZSM-5. The 10% Ni/ZSM-5 catalyst has about 72% hydrogen selectivity at temperature 873 K. It indicates that Ni is a more sustainable catalyst as compared to Co with ZSM-5 support for ESR. The C_2H_4 was found major undesirable products up to 823 K temperature. Nevertheless, the 10% Ni/ZSM-5 catalyst had shown its stability for high temperature(873 K) ESR performance.展开更多
Neuropathic pain is triggered by the lesions to peripheral nerves which alter their structure and function. Neuroprotective approaches that jimit the pathological changes and improve the behavioral outcome have been w...Neuropathic pain is triggered by the lesions to peripheral nerves which alter their structure and function. Neuroprotective approaches that jimit the pathological changes and improve the behavioral outcome have been well explained in different experimental models of neuropathy but translation of such strategies to clinics has been disappointing. Experimental evidences revealed the role of free radicals, especially per- oxynitrite after the nerve injury. They provoke oxidative DNA damage and consequent over-activation of the poly（ADP-ribose） polymerase （PARP） upregulates pro-inflammatory pathways, causing bioenergetic crisis and neuronal death. Along with these changes, it causes mitochondrial dysfunction leading to neu- ronal apoptosis. In related preclinical studies agents that neutralize the free radicals and pharmacological inhibitors of PARP have shown benefits in treating experimental neuropathy. This article reviews the in- volvement of PARP over-activation in trauma induced neuropathy and therapeutic significance of PARP inhibitors in the experimental neuropathy and neuropathic pain.展开更多
In monocytic cells, Toll-like receptor 4 （TLR4）- and TLR2-induced reactive oxygen species （ROS） cause oxidative stress and inflammatory response; however, the mechanism is not well understood. The present study in...In monocytic cells, Toll-like receptor 4 （TLR4）- and TLR2-induced reactive oxygen species （ROS） cause oxidative stress and inflammatory response; however, the mechanism is not well understood. The present study investigated the role of interleukin-1 receptor-associated kinase （IRAK）, extracellular signal-regulated kinase （ERK）, p67phox and Nox-2 in TLR4- and TLR2-induced ROS generation during interleukin-1 beta （IL-1β） transcription, processing, and secretion. An IRAK1/4 inhibitor, U0126, PD98059, an NADPH oxidase inhibitor （diphenyleneiodonium （DPI））, and a free radical scavenger （N-acetyl cysteine （NAC））-attenuated TLR4 （lipopolysaccharide （LPS））- and TLR2 （Pam3csk4）-induced ROS generation and IL-1β production in THP-1 and primary human monocytes. An IRAK1/4 inhibitor and siRNA-attenuated LPS- and Pam3csk4-induced ERK-IRAK1 association and ERK phosphorylation and activity. LPS and Pam3csk4 also induced IRAK1/4-, ERK- and ROS-dependent activation of activator protein-1 （AP-1）, IL-1β transcription, and IL-1β processing because significant inhibition in AP-1 activity, IL-lp transcription, Pro- and mature IL-p expression, and caspase-1 activity was observed with PD98059, U0126, DPI, NAC, an IRAK1/4 inhibitor, tanshinone Ila, and IRAK1 siRNA treatment. IRAK-dependent ERK-p67phox interaction, p67phox translocation, and p67phox-Nox-2 interaction were observed. Nox-2 siRNA significantly reduced secreted IL-lp, IL-1β transcript, pro- and mature IL-1β expression, and caspase-1 activity indicating a role for Nox-2 in LPS- and Pam3csk4-induced IL-1β production, transcription, and processing. In the present study, we demonstrate that the TLR4- and TLR2-induced IRAK-ERK pathway cross-talks with p67phox-Nox-2 for ROS generation, thus regulating IL-1β transcription and orocessin; in monocvtic cells.展开更多
High-entropy concept introduced with a promising paradigm to obtain exotic physical properties has motivated us to explore the thermoelectric properties of Sr-substituted high-entropy rare-earth cobaltates i.e.,(LaNdP...High-entropy concept introduced with a promising paradigm to obtain exotic physical properties has motivated us to explore the thermoelectric properties of Sr-substituted high-entropy rare-earth cobaltates i.e.,(LaNdPrSmEu)_(1-x)Sr_(x)CoO_(3)(0≤x≤0.10).The structural analysis of the samples,synthesized using the standard solid-state route,confirms the orthorhombic structure with the Pbnm space group.The Seebeck coefficient and electrical resistivity decrease with rising Sr concentration as well as with an increase in temperature.The multiple A-site ions in high-entropy rare-earth cobaltates result in an improved Seebeck coefficient(α)compared to La_(0.95)Sr_(0.05)CoO_(3),associated with a decrease in the Cosingle bondOsingle bondCo bond angle,which further enhances the power factor.The random distribution of cations at the rare-earth site results in a significant lowering of phonon thermal conductivity.As a result,a maximum figure of merit(zT)of 0.23 is obtained at 350K for(LaNdPrSmEu)_(0.95)Sr_(0.05)CoO_(3),which is one of the highest values of zT reported at this temperature for oxide materials.This study shows promise to decouple thermoelectric parameters using the high-entropy concept in several materials.展开更多
In this article,published online on 31 August 2015,there was an unintended error during image processing of Fig.2b leading to pasting of total ERK andβ-Actin blots not intended for this experiment.These blots have no...In this article,published online on 31 August 2015,there was an unintended error during image processing of Fig.2b leading to pasting of total ERK andβ-Actin blots not intended for this experiment.These blots have now been replaced with the total ERK andβ-Actin panels that were generated for this experiment.The corrected Fig.2b is shown here.However,the conclusion made from the experiment remains unaltered,and this correction has no bearing on the final outcome of the study.The inconvenience caused by this error is deeply regretted.展开更多
文摘This paper builds on exploring the applications of biomediated pathways to solve geotechnical challenges.First,the state of the art of biological remediation strategies including microbial remediation and phytoremediation have been introduced and critically reviewed in the context of decontaminating the soils.Next,biopolymerisation,biomineralisation and bioneutralisation processes have been depicted with a special emphasis on the applications including but not limited to soil stabilisation,soil erosion prevention,anti-desertification and pH neutralisation.Each of these methods have their own limitations and bottlenecks while scaling up,and these challenges have been summarised and some possible paths to overcome the challenges have also been discussed.The state of the art of electromagnetic(EM)monitoring methods to capture the effects of biomediation on spatio-temporal soil properties are then highlighted as a non-invasive and rapid pathway to track the progress of biomediated soil processes.Finally,each of the technologies discussed have been evaluated for their maturity level using the principles of technology readiness level(TRL).A majority of the technologies amounting to around 77%are still in the TRL 4e7,i.e.in the valley of death.It is thus evident that development of these technologies needs to be supported with appropriate funding for improving their maturity to a level of industrial deployment.
基金Institute of Technology (BHU) Varanasi and MHRD, Govt. of India for financial assistance (TA) to Mr Ashutosh Kumar
文摘The renewable hydrogen generation through ethanol steam reforming is one of the anticipated areas for sustainable hydrogen generation. To elucidate the role of Ni and Co with ZSM-5 support, catalysts were prepared by wet impregnation method and ethanol steam reforming(ESR) was performed. The catalysts were characterized by HR-XRD, ATR–FTIR, HR-SEM, TEM with SAED, EDAX, surface area analyzer and TPR. It had shown complete ethanol conversion at 773 K, but the selectivity in hydrogen generation was found higher for 10% Ni/ZSM-5 catalyst as compared to 10% Co/ZSM-5. The 10% Ni/ZSM-5 catalyst has about 72% hydrogen selectivity at temperature 873 K. It indicates that Ni is a more sustainable catalyst as compared to Co with ZSM-5 support for ESR. The C_2H_4 was found major undesirable products up to 823 K temperature. Nevertheless, the 10% Ni/ZSM-5 catalyst had shown its stability for high temperature(873 K) ESR performance.
基金Department of Biotechnology Govt of India,for their financial support to Dr.Ashutosh Kumar via grant BT/527/NE/TBP/2013the financial support from Department of Pharmaceuticals,Ministry of Chemical and Fertilizers and NIPER Hyderabad for their support
文摘Neuropathic pain is triggered by the lesions to peripheral nerves which alter their structure and function. Neuroprotective approaches that jimit the pathological changes and improve the behavioral outcome have been well explained in different experimental models of neuropathy but translation of such strategies to clinics has been disappointing. Experimental evidences revealed the role of free radicals, especially per- oxynitrite after the nerve injury. They provoke oxidative DNA damage and consequent over-activation of the poly（ADP-ribose） polymerase （PARP） upregulates pro-inflammatory pathways, causing bioenergetic crisis and neuronal death. Along with these changes, it causes mitochondrial dysfunction leading to neu- ronal apoptosis. In related preclinical studies agents that neutralize the free radicals and pharmacological inhibitors of PARP have shown benefits in treating experimental neuropathy. This article reviews the in- volvement of PARP over-activation in trauma induced neuropathy and therapeutic significance of PARP inhibitors in the experimental neuropathy and neuropathic pain.
文摘In monocytic cells, Toll-like receptor 4 （TLR4）- and TLR2-induced reactive oxygen species （ROS） cause oxidative stress and inflammatory response; however, the mechanism is not well understood. The present study investigated the role of interleukin-1 receptor-associated kinase （IRAK）, extracellular signal-regulated kinase （ERK）, p67phox and Nox-2 in TLR4- and TLR2-induced ROS generation during interleukin-1 beta （IL-1β） transcription, processing, and secretion. An IRAK1/4 inhibitor, U0126, PD98059, an NADPH oxidase inhibitor （diphenyleneiodonium （DPI））, and a free radical scavenger （N-acetyl cysteine （NAC））-attenuated TLR4 （lipopolysaccharide （LPS））- and TLR2 （Pam3csk4）-induced ROS generation and IL-1β production in THP-1 and primary human monocytes. An IRAK1/4 inhibitor and siRNA-attenuated LPS- and Pam3csk4-induced ERK-IRAK1 association and ERK phosphorylation and activity. LPS and Pam3csk4 also induced IRAK1/4-, ERK- and ROS-dependent activation of activator protein-1 （AP-1）, IL-1β transcription, and IL-1β processing because significant inhibition in AP-1 activity, IL-lp transcription, Pro- and mature IL-p expression, and caspase-1 activity was observed with PD98059, U0126, DPI, NAC, an IRAK1/4 inhibitor, tanshinone Ila, and IRAK1 siRNA treatment. IRAK-dependent ERK-p67phox interaction, p67phox translocation, and p67phox-Nox-2 interaction were observed. Nox-2 siRNA significantly reduced secreted IL-lp, IL-1β transcript, pro- and mature IL-1β expression, and caspase-1 activity indicating a role for Nox-2 in LPS- and Pam3csk4-induced IL-1β production, transcription, and processing. In the present study, we demonstrate that the TLR4- and TLR2-induced IRAK-ERK pathway cross-talks with p67phox-Nox-2 for ROS generation, thus regulating IL-1β transcription and orocessin; in monocvtic cells.
基金This work was supported by the French Agence Nationale de la Recherche(ANR),through the project NEO(ANR 19-CE30-0030-01).
文摘High-entropy concept introduced with a promising paradigm to obtain exotic physical properties has motivated us to explore the thermoelectric properties of Sr-substituted high-entropy rare-earth cobaltates i.e.,(LaNdPrSmEu)_(1-x)Sr_(x)CoO_(3)(0≤x≤0.10).The structural analysis of the samples,synthesized using the standard solid-state route,confirms the orthorhombic structure with the Pbnm space group.The Seebeck coefficient and electrical resistivity decrease with rising Sr concentration as well as with an increase in temperature.The multiple A-site ions in high-entropy rare-earth cobaltates result in an improved Seebeck coefficient(α)compared to La_(0.95)Sr_(0.05)CoO_(3),associated with a decrease in the Cosingle bondOsingle bondCo bond angle,which further enhances the power factor.The random distribution of cations at the rare-earth site results in a significant lowering of phonon thermal conductivity.As a result,a maximum figure of merit(zT)of 0.23 is obtained at 350K for(LaNdPrSmEu)_(0.95)Sr_(0.05)CoO_(3),which is one of the highest values of zT reported at this temperature for oxide materials.This study shows promise to decouple thermoelectric parameters using the high-entropy concept in several materials.
文摘In this article,published online on 31 August 2015,there was an unintended error during image processing of Fig.2b leading to pasting of total ERK andβ-Actin blots not intended for this experiment.These blots have now been replaced with the total ERK andβ-Actin panels that were generated for this experiment.The corrected Fig.2b is shown here.However,the conclusion made from the experiment remains unaltered,and this correction has no bearing on the final outcome of the study.The inconvenience caused by this error is deeply regretted.
