Fermented mustard seed is a unique delicacy from Assam,India,with many health benefits.The fermentation process involves a complex interplay of various microbes that give the final product its desired characteristics....Fermented mustard seed is a unique delicacy from Assam,India,with many health benefits.The fermentation process involves a complex interplay of various microbes that give the final product its desired characteristics.A comprehensive understanding of fermented mustard’s microbial and metabolite composition can aid in optimizing the fermentation process,enabling the retention of maximum beneficial effects.This study reports the traditional fermentation process of mustard seeds,the composition of its native microbe-metabolite,and the development of a starter culture that increases the polyunsaturated fatty acid(PUFA)content.The fermented mustard seeds have a complex bacterial ecosystem comprising of Staphylococcus,Bacillus,Mammaliicoccus,Weisella,Corynebacterium,Aerococcus,Ruoffia,Paenibacillus,and Enterococcus.Moreover,untargeted metabolomics reveals that it is a rich source of functional metabolites,especially PUFA.Based on multi-omics approaches,the study deduced the core bacterial composition to be S.succinus,S.saprophyticus,and B.subtilis,with a strong positive correlation(p<0.05)with the synthesis of PUFA.Optimized fermentation experiments of mustard seed with the core bacterial group resulted in an enhanced PUFA content of 52.9%.Transcriptome study on HT-29 cells treated with optimized fermented mustard seed extract exhibited differential expression of genes linked to cellular pathways associated with gastrointestinal health,such as gut homeostasis,intestinal barrier integrity,immuno-modulatory,and neuronal development.This study revealed an omics-guided strategy for the development of starter-assisted controlled fermentation with experimental validation on fermented mustard seed.展开更多
基金Department of Science and Technology(DST),Govt.of India(GOI)for funding the project entitled“ST/SC community development programme in IASST”vide letter no.SEED/TITE/2019/103/G dtd:March 12,2020SEED/TITE/2019/103/C dtd:March 12,2020and SEED/TITE/2019/103/C dtd:September 21,2020.
文摘Fermented mustard seed is a unique delicacy from Assam,India,with many health benefits.The fermentation process involves a complex interplay of various microbes that give the final product its desired characteristics.A comprehensive understanding of fermented mustard’s microbial and metabolite composition can aid in optimizing the fermentation process,enabling the retention of maximum beneficial effects.This study reports the traditional fermentation process of mustard seeds,the composition of its native microbe-metabolite,and the development of a starter culture that increases the polyunsaturated fatty acid(PUFA)content.The fermented mustard seeds have a complex bacterial ecosystem comprising of Staphylococcus,Bacillus,Mammaliicoccus,Weisella,Corynebacterium,Aerococcus,Ruoffia,Paenibacillus,and Enterococcus.Moreover,untargeted metabolomics reveals that it is a rich source of functional metabolites,especially PUFA.Based on multi-omics approaches,the study deduced the core bacterial composition to be S.succinus,S.saprophyticus,and B.subtilis,with a strong positive correlation(p<0.05)with the synthesis of PUFA.Optimized fermentation experiments of mustard seed with the core bacterial group resulted in an enhanced PUFA content of 52.9%.Transcriptome study on HT-29 cells treated with optimized fermented mustard seed extract exhibited differential expression of genes linked to cellular pathways associated with gastrointestinal health,such as gut homeostasis,intestinal barrier integrity,immuno-modulatory,and neuronal development.This study revealed an omics-guided strategy for the development of starter-assisted controlled fermentation with experimental validation on fermented mustard seed.
