We studied the genetic polymorphism among 29 clones of shisham （Dalbergia sissoo Roxb） belonging to different geographic regions using random amplified polymorphic DNA （RAPD） markers. Out of 30 primers used, only ...We studied the genetic polymorphism among 29 clones of shisham （Dalbergia sissoo Roxb） belonging to different geographic regions using random amplified polymorphic DNA （RAPD） markers. Out of 30 primers used, only 20 primers generated polymorphism in amplified product. In total 232 bands were amplified with 20 primers, of which 192 （82%） were polymorphic with an average of 9.6 bands/primer. The resolving power （Rp） ranged from 2.14 （Primer 5） to 11.93 （Primer 4）. Primer 4 and Primer 3 possessed high Rp value. Polymorphism information content （PIC） ranged from 0.15 （Primer 5） to 0.37 （Primer 4）. Primer 4 amplified total 18 bands in 29 genotypes with PIC value of 0.37 hence; this set of primer was most informative. The similarity coefficient analysis revealed two clusters. The first cluster comprised of only 10 clones and the second major cluster comprised of 19 clones. The genetic similarity among 29 clones ranged from 25.86% （clone 10 and 235） to 100% （clone 19 and 59）, suggesting a wide genetic base in shisham clones.展开更多
The study of genetic variability is a prerequisite for any tree improvement program. Screening of clones showing the effect of dominance in growth and tree form is essential to identify productive clones, matched to s...The study of genetic variability is a prerequisite for any tree improvement program. Screening of clones showing the effect of dominance in growth and tree form is essential to identify productive clones, matched to sites. Our study performed in a clonal seed orchard at Lacchiwala of India, represented by 31 clones of Dalbergia sissoo （Shisham） Roxb. from diverse locations revealed significant variability at the 0.1% level of significance in various morphological traits. At the age of 13 years, clone 196 （Gonda） revealed maximum height of 20.2 m and a girth at breast height of 74.2 cm. The five identified clones viz. 196, 198, 192, 123 and 235 were well adapted to the climatic conditions of this zone, hence survived well and showed good performance compared to other clones. Heritability estimates of 87%, 83% and 80% for height, crown width and crown length respectively coupled with high genetic gains indicated strong genetic control of these variables. A correlation matrix of various growth attributes revealed positive correlation among these attributes. On the basis of the entire set of growth variables, the clones were grouped into two major clusters with three subgroups.展开更多
Stroke,a leading cause of mortality and morbidity worldwide,is a complex cerebrovascular disease.Stroke risk factors are diverse,encompassing age,sex,and ethnicity.Comorbid conditions,including hypertension,hyperglyce...Stroke,a leading cause of mortality and morbidity worldwide,is a complex cerebrovascular disease.Stroke risk factors are diverse,encompassing age,sex,and ethnicity.Comorbid conditions,including hypertension,hyperglycemia,hyperlipidemia,and atrial fibrillation,exacerbate stroke outcomes,contributing to the overall stroke burden within populations.In addition to these factors,lifestyle-related diseases can impact individuals across all age groups,and often include as comorbidities linked to stroke.Socioeconomic conditions,healthcare access,and the quality of clinical data significantly influence the prevalence of comorbidities.Asia,the largest continent and home to 60%of the world's population,includes many developing nations undergoing diverse economic transitions.In Southeast Asian countries,stroke prevalence is high,imposing a substantial burden on healthcare systems and economies.Research disparities in stroke are often attributed to insufficient demographic data on comorbidities.Hence,the review discusses all previously published results of hospital-based studies and data from national registries.It has been noticed that due to insufficient documentation on stroke-related comorbidities in various developing countries of Southeast Asia,stroke management becomes difficult.Therefore,this review aims to discuss the association between various comorbidities and stroke,with special emphasis on the incidence and prevalence of stroke burden in Southeast Asian countries.展开更多
文摘We studied the genetic polymorphism among 29 clones of shisham （Dalbergia sissoo Roxb） belonging to different geographic regions using random amplified polymorphic DNA （RAPD） markers. Out of 30 primers used, only 20 primers generated polymorphism in amplified product. In total 232 bands were amplified with 20 primers, of which 192 （82%） were polymorphic with an average of 9.6 bands/primer. The resolving power （Rp） ranged from 2.14 （Primer 5） to 11.93 （Primer 4）. Primer 4 and Primer 3 possessed high Rp value. Polymorphism information content （PIC） ranged from 0.15 （Primer 5） to 0.37 （Primer 4）. Primer 4 amplified total 18 bands in 29 genotypes with PIC value of 0.37 hence; this set of primer was most informative. The similarity coefficient analysis revealed two clusters. The first cluster comprised of only 10 clones and the second major cluster comprised of 19 clones. The genetic similarity among 29 clones ranged from 25.86% （clone 10 and 235） to 100% （clone 19 and 59）, suggesting a wide genetic base in shisham clones.
文摘The study of genetic variability is a prerequisite for any tree improvement program. Screening of clones showing the effect of dominance in growth and tree form is essential to identify productive clones, matched to sites. Our study performed in a clonal seed orchard at Lacchiwala of India, represented by 31 clones of Dalbergia sissoo （Shisham） Roxb. from diverse locations revealed significant variability at the 0.1% level of significance in various morphological traits. At the age of 13 years, clone 196 （Gonda） revealed maximum height of 20.2 m and a girth at breast height of 74.2 cm. The five identified clones viz. 196, 198, 192, 123 and 235 were well adapted to the climatic conditions of this zone, hence survived well and showed good performance compared to other clones. Heritability estimates of 87%, 83% and 80% for height, crown width and crown length respectively coupled with high genetic gains indicated strong genetic control of these variables. A correlation matrix of various growth attributes revealed positive correlation among these attributes. On the basis of the entire set of growth variables, the clones were grouped into two major clusters with three subgroups.
基金Department of Pharmaceuticals,Ministry of Chemical and Fertilizers,Government of IndiaIndian Council of Medical Research(ICMR),New Delhi,India,for the senior research fellowship grant to Ms.Aishika Datta(45/13/2020-PHA/BMS)ICMR Nanobio project grant to Dr.Pallab Bhattacharya(34/5/2019-TF/Nano/BMS).
文摘Stroke,a leading cause of mortality and morbidity worldwide,is a complex cerebrovascular disease.Stroke risk factors are diverse,encompassing age,sex,and ethnicity.Comorbid conditions,including hypertension,hyperglycemia,hyperlipidemia,and atrial fibrillation,exacerbate stroke outcomes,contributing to the overall stroke burden within populations.In addition to these factors,lifestyle-related diseases can impact individuals across all age groups,and often include as comorbidities linked to stroke.Socioeconomic conditions,healthcare access,and the quality of clinical data significantly influence the prevalence of comorbidities.Asia,the largest continent and home to 60%of the world's population,includes many developing nations undergoing diverse economic transitions.In Southeast Asian countries,stroke prevalence is high,imposing a substantial burden on healthcare systems and economies.Research disparities in stroke are often attributed to insufficient demographic data on comorbidities.Hence,the review discusses all previously published results of hospital-based studies and data from national registries.It has been noticed that due to insufficient documentation on stroke-related comorbidities in various developing countries of Southeast Asia,stroke management becomes difficult.Therefore,this review aims to discuss the association between various comorbidities and stroke,with special emphasis on the incidence and prevalence of stroke burden in Southeast Asian countries.
