Lithium metal batteries(LMBs)have attracted huge attention due to super-high capacity and low reduction potential of lithium anode constructing high-energy/power density.However,the practical application of LMBs is si...Lithium metal batteries(LMBs)have attracted huge attention due to super-high capacity and low reduction potential of lithium anode constructing high-energy/power density.However,the practical application of LMBs is significantly constrained by lithium dendrite growth and high reactivity of lithium anode.Herein,a novel functionalized interlayer that SbF3 is tandem on HKUST-1 skeleton forming favorable Sb-terminated groups structure(HKSF@PE),which were proposed and fabricated to construct highly stable LMBs.Theoretical calculations demonstrate that the Sb-terminated groups structure in this configuration display strong interaction with lithium,which can act as a cation receptor and adsorption sites,thereby promoting lithium-ion desolvation and improving lithium-ion transport kinetics.Meanwhile,in-situ XRD,Raman,and DRT analyses indicate that the HKSF assist the formation of LiF-rich and lithiophilic Li3Sb alloys at SEI/Li interface,regulating lithium depo-sition morphology and reconstructing a reinforced SEI interlayer.Consequently,Li|HKSF@PE|Li symmetric cell exhibits exceptional stability over 2500 h at 2 mA cm^(-2) with 1 mAh cm^(-2),and Li|HKSF@PE|LFP full cell demonstrates a high-capacity retention of 92.0%after 220 cycles even at a high rate of 5C.This work reveals the important role of terminated groups to achieve homogeneous lithium deposition and provide a way to construct stable LMBs.展开更多
为了研究砂藓bZIP转录因子基因RcbZIP3和RcbZIP4的功能,本研究利用实时荧光定量PCR技术,对砂藓RcbZIP3和RcbZIP4基因在非生物胁迫下的表达进行分析,并构建了植物表达载体pRI-101-AN-RcbZIP3和p RI 101-AN-RcbZIP4。结果表明,RcbZIP3在...为了研究砂藓bZIP转录因子基因RcbZIP3和RcbZIP4的功能,本研究利用实时荧光定量PCR技术,对砂藓RcbZIP3和RcbZIP4基因在非生物胁迫下的表达进行分析,并构建了植物表达载体pRI-101-AN-RcbZIP3和p RI 101-AN-RcbZIP4。结果表明,RcbZIP3在干旱、高温和低温处理下表达量均升高,在水渍处理下表达量先上升后下降再上升;RcbZIP4在干旱、高温和水渍处理下表达量均升高,在低温处理下表达量先下降后上升。RcbZIP3和RcbZIP4基因成功构建到植物表达载体pRI 101-AN上。通过农杆菌介导法对烟草进行遗传转化,PCR检测鉴定阳性转化植株,证明RcbZIP3和RcbZIP4基因已整合到烟草的基因组中。本试验为进一步研究砂藓RcbZIP3和RcbZIP4基因的生物学功能提供了科学依据。展开更多
[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rat...[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling,arthritis score,immune organ index and hematoxylin-eosin( HE) staining. The content of inflammatory factors TNF-α,IL-6,and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay( ELISA) respectively. [Results]Compared with the model group,the total extract of K. coccinea( KCTE) could significantly inhibit the primary and secondary articular swelling of AA rats,lower the arthritis score and immune organ index,and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE( KCTE-H and KCTE-M),KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats,exhibiting a certain dose-effect relationship. [Conclusions]KCTE had significant therapeutic effect on the AA of rats,and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.展开更多
OBJECTIVE To probe into the anti-esophagus cancer activity and mechanisms of DN3,a novel natural diterpenoid derivative.METHODS The anti-tumor activity in vitro of DN3 was evaluated by MTT,and by using human esophagea...OBJECTIVE To probe into the anti-esophagus cancer activity and mechanisms of DN3,a novel natural diterpenoid derivative.METHODS The anti-tumor activity in vitro of DN3 was evaluated by MTT,and by using human esophageal carcinoma cells xenografted into athymic mice model in vivo.The specific mechanisms of DN3,as a dual inhibitor of glycolysis and oxidative phos.phorylation(OXPHOS) were explored through cell and molecular biology techniques.For instance,the manner of cancer cell death induced by DN3 was characterized by hoechst33342,FITC-Annexin V/PI staining and flow cytometric analysis,then these changes of glucose consumption,glucose uptake and lactate production in glycolysis,as well as oxygen consumption rate(OCR) and ATP content in OXPHOS caused by DN3 were performed separately through related kits and SeahorseBioscience XF24 Extra.cellular Flux Analyzer.Furthermore,in order to obtain a clear understanding of the inhibition of DN3 to glycolysis and OXPHOS,these regulatory factors were investigated by Western blot,such as PI3K/AKT,c-Myc and p53 of glycolysis,Bax and HK2 of mitochondrial function.RESULTS DN3 inhibited the growth of esophagus cancer cell EC9706,EC109 and EC1 cells in a dose and time dependent manner,but showed no significant effects on human esophageal epithelial cells(HEECs).DN3 caused significant G2/M arrest of esophagus cancer cell lines and induced apoptosis of these cell lines,which indicated DN3 inhibited the growth of esophagus cancer cell through blocking cell cycle and inducing apoptosis in a dose and time-dependent manner.Importantly,8 μM DN3 decreased the extracellular acidification rate(ECAR) by 45% in EC109,which indicated glycolysis was inhibited by DN3.Mean.while,DN3 decreased the oxygen consumption rate(OCR) and the OCR linked to intracellular ATP production in EC109 cells,but that was not obvious in HEECs,so which indicated that DN3 could selec.tively block OXPHOS of cancer cells.In addition,the accumulation of reactive oxygen species(ROS)and the drop of mitochondrial membrane potential(MMP) were also observed in EC109 incubated by DN3,which suggested mitochondrial biological function was disturbed.Furthermore,the expression of PI3K/AKT,c-Myc and HK2 related to glycolysis were down-regulated by DN3,but the p53 and Bax were up-regulated in esophageal carcinoma cells.The changes of these enzymes accounted for the decreased glycolysisand OXPHOS in esophageal carcinoma cells treated by DN3.CONCLUSION The new compound DN3 has a strong anti-esophageal carcinoma activity,and it is tolerable that DN3 is seen as a dual inhibitor of glycolysis and oxidative phosphorylation.展开更多
OBJECTIVE To study the anti-tumor activity and molecular mechanism of natural diter.pene derivative JD20 in vitro.METHODS Screening the sensitive of gastric carcinoma cell lines to JD20 by cytotoxicity test for 24 h.C...OBJECTIVE To study the anti-tumor activity and molecular mechanism of natural diter.pene derivative JD20 in vitro.METHODS Screening the sensitive of gastric carcinoma cell lines to JD20 by cytotoxicity test for 24 h.Cell morphology was evaluated by using DAPI.After staining of can.cer cells with PI or annexin V-FITC/PI respectively,the cell cycle and apoptosis induced by JD20 were detectded by flow cytometry.The change in cell membrane potential was detected by JC-1 test kit.Western blot method was used to detect the apoptosis-related protein.RESULTS The novel natural kaurane diterpene derivative JD20 had a significant inhibitory effect on tumor cells and was particularly active on gastric cancer cell lines HGC-27(IC50=4.72±1.37 μmol·L-1) and MGC-803(IC50=7.36±0.86 μmol·L^(-1)).Further studies found that JD20 resulted in thecell cycle arrest in the G2/M phase,and induced a significant apoptosis in HGC-27.In addition,JD20 also caused the drop of mitochondrial membrane potential of HGC-27 within a short time(3 h).Furthermore,the Western blotting analysis showed that JD20 could induce the up-regulation of p53,Bax and Bim protein expression in gastric can.cer cells,and the releasing of cytochrome c from the mitochondria into the cytoplasm,as well as the ac.tivation of casepase-9/3.CONCLUSION The natural kaurane diterpene derivative JD20 can inhibit the proliferation of various human cancer cells by blocking the cell cycle and inducing apoptosis,and its mechanism of inducing apoptosis may be related to the mitochondria-mediated apoptosis pathway.展开更多
OBJECTIVE Gastric cancer is one of the most common malignant tumors,and the incidence rate is the highest in all kinds of tumors in China.However,it remains unclear that how signifi.cantly gastric cells are dependent ...OBJECTIVE Gastric cancer is one of the most common malignant tumors,and the incidence rate is the highest in all kinds of tumors in China.However,it remains unclear that how signifi.cantly gastric cells are dependent on glycolysis,and which type of gastric cells are sensitive to glycolysis inhibition.In this study,several kind of gastric cancer cell lines were used as the research object,and the metabolic characteristics of different cell lines were systematically analyzed to provide theoretical support for the accurate treatment of gastric cancer.METHODS We examined the energy metabolism of four gastric cancer cell lines(MGC-803,SGC-7901,HGC-27 and BGC-823) by using glycolysis inhibitor,2-deoxy-D-glucose(2-DG) and inhibitor of oxidative phosphorylation,oligomycin.Oxygen consumption rates(OCR) and L-lactate were also measured with an XF96 Analyzer(Seahorse Biosciences) to deter.mine the significance of metabolism of oxidative phosphorylation and aerobic glycolysisin gastric cells.In addition,western blot was used to detect the contribution of AMP-activated protein kinase(AMPK),and anti-apoptotic proteins(Bcl-2 and survivin) to clarify the mechanism of death or survival of gastric cancer cells treated by 2-DG or oligomycin.RESULTS In this study,it was shown that the growth of gastric cell lines were suppressed by 2-DG.However,the sensitivity to 2-DG was quite different among cell lines:IC 50 of 2-DG was from 3.28 mmol·L^(-1)(MGC-803) to 15.57 mmol·L^(-1)(BGC-823).MGC-803 was relatively sensitive to 2-DG(IC 50:3.28 mmol·L^(-1)),consumed more glucose and produced more lactate(waste product of glycolysis) than the three other cell lines.Consequently,MGC-803 could be more dependent on glycolysis than other cell lines,which was further confirmed by the fact that glucose(+) FCS(-) medium showed more growth and survival than glucose(-) FCS(+) medium.Alternatively,BGC-823,most resistant to 2-DG(IC50:15.57 mmol · L-1),was most sensitive to oligomycin,and showed more growth and survival in glucose(-) FCS(+) medium than in glucose(+) FCS(-) medium.Thus,we had reasons to think BGC-823 cells depended on oxidative phosphorylation for energy production.In BGC-823,AMPK,which is activated when ATP becomes limiting,was rapidly phosphorylated by 2-DG,and expression of Bcl-2 was augmented,which might result in resistance to 2-DG.Interestingly,AMPK phosphorylation and augmentation of Bcl-2 expression by 2-DG were not observed in MGC-803,which is 2-DG sensitive.CONCLUSION There is a large metabolic difference between gastric cancer cell lines,which will facilitate the future gastric cancer therapy by targeting metabolic pathways.展开更多
基金supported by National Natural Science Foundation of China(52372180,92572105)The Natural Science Foundation of Jiangsu Province(BK20250069)+3 种基金Project on Carbon Emission Peak and Neutrality of Jiangsu Province(BE2022031-4)the Fundamental Research Funds for the Central Universities(2242022K40001)The Start-up Research Fund of Southeast University(RF1028623081)Natural Science Foundation of Chongqing(CSTB2025NSCQ-GPX0662).
文摘Lithium metal batteries(LMBs)have attracted huge attention due to super-high capacity and low reduction potential of lithium anode constructing high-energy/power density.However,the practical application of LMBs is significantly constrained by lithium dendrite growth and high reactivity of lithium anode.Herein,a novel functionalized interlayer that SbF3 is tandem on HKUST-1 skeleton forming favorable Sb-terminated groups structure(HKSF@PE),which were proposed and fabricated to construct highly stable LMBs.Theoretical calculations demonstrate that the Sb-terminated groups structure in this configuration display strong interaction with lithium,which can act as a cation receptor and adsorption sites,thereby promoting lithium-ion desolvation and improving lithium-ion transport kinetics.Meanwhile,in-situ XRD,Raman,and DRT analyses indicate that the HKSF assist the formation of LiF-rich and lithiophilic Li3Sb alloys at SEI/Li interface,regulating lithium depo-sition morphology and reconstructing a reinforced SEI interlayer.Consequently,Li|HKSF@PE|Li symmetric cell exhibits exceptional stability over 2500 h at 2 mA cm^(-2) with 1 mAh cm^(-2),and Li|HKSF@PE|LFP full cell demonstrates a high-capacity retention of 92.0%after 220 cycles even at a high rate of 5C.This work reveals the important role of terminated groups to achieve homogeneous lithium deposition and provide a way to construct stable LMBs.
文摘为了研究砂藓bZIP转录因子基因RcbZIP3和RcbZIP4的功能,本研究利用实时荧光定量PCR技术,对砂藓RcbZIP3和RcbZIP4基因在非生物胁迫下的表达进行分析,并构建了植物表达载体pRI-101-AN-RcbZIP3和p RI 101-AN-RcbZIP4。结果表明,RcbZIP3在干旱、高温和低温处理下表达量均升高,在水渍处理下表达量先上升后下降再上升;RcbZIP4在干旱、高温和水渍处理下表达量均升高,在低温处理下表达量先下降后上升。RcbZIP3和RcbZIP4基因成功构建到植物表达载体pRI 101-AN上。通过农杆菌介导法对烟草进行遗传转化,PCR检测鉴定阳性转化植株,证明RcbZIP3和RcbZIP4基因已整合到烟草的基因组中。本试验为进一步研究砂藓RcbZIP3和RcbZIP4基因的生物学功能提供了科学依据。
基金Supported by National Natural Science Foundation of China(No.81303162)China Postdoctoral Science Foundation(No.2015T80784,2014M552017)Postdoctoral Scientific Research Project of Henan Province(No.2013010)
文摘[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling,arthritis score,immune organ index and hematoxylin-eosin( HE) staining. The content of inflammatory factors TNF-α,IL-6,and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay( ELISA) respectively. [Results]Compared with the model group,the total extract of K. coccinea( KCTE) could significantly inhibit the primary and secondary articular swelling of AA rats,lower the arthritis score and immune organ index,and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE( KCTE-H and KCTE-M),KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats,exhibiting a certain dose-effect relationship. [Conclusions]KCTE had significant therapeutic effect on the AA of rats,and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.
基金supported by National Natural Science Foundation of China(81502952)
文摘OBJECTIVE To probe into the anti-esophagus cancer activity and mechanisms of DN3,a novel natural diterpenoid derivative.METHODS The anti-tumor activity in vitro of DN3 was evaluated by MTT,and by using human esophageal carcinoma cells xenografted into athymic mice model in vivo.The specific mechanisms of DN3,as a dual inhibitor of glycolysis and oxidative phos.phorylation(OXPHOS) were explored through cell and molecular biology techniques.For instance,the manner of cancer cell death induced by DN3 was characterized by hoechst33342,FITC-Annexin V/PI staining and flow cytometric analysis,then these changes of glucose consumption,glucose uptake and lactate production in glycolysis,as well as oxygen consumption rate(OCR) and ATP content in OXPHOS caused by DN3 were performed separately through related kits and SeahorseBioscience XF24 Extra.cellular Flux Analyzer.Furthermore,in order to obtain a clear understanding of the inhibition of DN3 to glycolysis and OXPHOS,these regulatory factors were investigated by Western blot,such as PI3K/AKT,c-Myc and p53 of glycolysis,Bax and HK2 of mitochondrial function.RESULTS DN3 inhibited the growth of esophagus cancer cell EC9706,EC109 and EC1 cells in a dose and time dependent manner,but showed no significant effects on human esophageal epithelial cells(HEECs).DN3 caused significant G2/M arrest of esophagus cancer cell lines and induced apoptosis of these cell lines,which indicated DN3 inhibited the growth of esophagus cancer cell through blocking cell cycle and inducing apoptosis in a dose and time-dependent manner.Importantly,8 μM DN3 decreased the extracellular acidification rate(ECAR) by 45% in EC109,which indicated glycolysis was inhibited by DN3.Mean.while,DN3 decreased the oxygen consumption rate(OCR) and the OCR linked to intracellular ATP production in EC109 cells,but that was not obvious in HEECs,so which indicated that DN3 could selec.tively block OXPHOS of cancer cells.In addition,the accumulation of reactive oxygen species(ROS)and the drop of mitochondrial membrane potential(MMP) were also observed in EC109 incubated by DN3,which suggested mitochondrial biological function was disturbed.Furthermore,the expression of PI3K/AKT,c-Myc and HK2 related to glycolysis were down-regulated by DN3,but the p53 and Bax were up-regulated in esophageal carcinoma cells.The changes of these enzymes accounted for the decreased glycolysisand OXPHOS in esophageal carcinoma cells treated by DN3.CONCLUSION The new compound DN3 has a strong anti-esophageal carcinoma activity,and it is tolerable that DN3 is seen as a dual inhibitor of glycolysis and oxidative phosphorylation.
基金supported by National Natural Science Foundation of China(81502952)
文摘OBJECTIVE To study the anti-tumor activity and molecular mechanism of natural diter.pene derivative JD20 in vitro.METHODS Screening the sensitive of gastric carcinoma cell lines to JD20 by cytotoxicity test for 24 h.Cell morphology was evaluated by using DAPI.After staining of can.cer cells with PI or annexin V-FITC/PI respectively,the cell cycle and apoptosis induced by JD20 were detectded by flow cytometry.The change in cell membrane potential was detected by JC-1 test kit.Western blot method was used to detect the apoptosis-related protein.RESULTS The novel natural kaurane diterpene derivative JD20 had a significant inhibitory effect on tumor cells and was particularly active on gastric cancer cell lines HGC-27(IC50=4.72±1.37 μmol·L-1) and MGC-803(IC50=7.36±0.86 μmol·L^(-1)).Further studies found that JD20 resulted in thecell cycle arrest in the G2/M phase,and induced a significant apoptosis in HGC-27.In addition,JD20 also caused the drop of mitochondrial membrane potential of HGC-27 within a short time(3 h).Furthermore,the Western blotting analysis showed that JD20 could induce the up-regulation of p53,Bax and Bim protein expression in gastric can.cer cells,and the releasing of cytochrome c from the mitochondria into the cytoplasm,as well as the ac.tivation of casepase-9/3.CONCLUSION The natural kaurane diterpene derivative JD20 can inhibit the proliferation of various human cancer cells by blocking the cell cycle and inducing apoptosis,and its mechanism of inducing apoptosis may be related to the mitochondria-mediated apoptosis pathway.
基金supported by National Natural Science Foundation of China(81502952)
文摘OBJECTIVE Gastric cancer is one of the most common malignant tumors,and the incidence rate is the highest in all kinds of tumors in China.However,it remains unclear that how signifi.cantly gastric cells are dependent on glycolysis,and which type of gastric cells are sensitive to glycolysis inhibition.In this study,several kind of gastric cancer cell lines were used as the research object,and the metabolic characteristics of different cell lines were systematically analyzed to provide theoretical support for the accurate treatment of gastric cancer.METHODS We examined the energy metabolism of four gastric cancer cell lines(MGC-803,SGC-7901,HGC-27 and BGC-823) by using glycolysis inhibitor,2-deoxy-D-glucose(2-DG) and inhibitor of oxidative phosphorylation,oligomycin.Oxygen consumption rates(OCR) and L-lactate were also measured with an XF96 Analyzer(Seahorse Biosciences) to deter.mine the significance of metabolism of oxidative phosphorylation and aerobic glycolysisin gastric cells.In addition,western blot was used to detect the contribution of AMP-activated protein kinase(AMPK),and anti-apoptotic proteins(Bcl-2 and survivin) to clarify the mechanism of death or survival of gastric cancer cells treated by 2-DG or oligomycin.RESULTS In this study,it was shown that the growth of gastric cell lines were suppressed by 2-DG.However,the sensitivity to 2-DG was quite different among cell lines:IC 50 of 2-DG was from 3.28 mmol·L^(-1)(MGC-803) to 15.57 mmol·L^(-1)(BGC-823).MGC-803 was relatively sensitive to 2-DG(IC 50:3.28 mmol·L^(-1)),consumed more glucose and produced more lactate(waste product of glycolysis) than the three other cell lines.Consequently,MGC-803 could be more dependent on glycolysis than other cell lines,which was further confirmed by the fact that glucose(+) FCS(-) medium showed more growth and survival than glucose(-) FCS(+) medium.Alternatively,BGC-823,most resistant to 2-DG(IC50:15.57 mmol · L-1),was most sensitive to oligomycin,and showed more growth and survival in glucose(-) FCS(+) medium than in glucose(+) FCS(-) medium.Thus,we had reasons to think BGC-823 cells depended on oxidative phosphorylation for energy production.In BGC-823,AMPK,which is activated when ATP becomes limiting,was rapidly phosphorylated by 2-DG,and expression of Bcl-2 was augmented,which might result in resistance to 2-DG.Interestingly,AMPK phosphorylation and augmentation of Bcl-2 expression by 2-DG were not observed in MGC-803,which is 2-DG sensitive.CONCLUSION There is a large metabolic difference between gastric cancer cell lines,which will facilitate the future gastric cancer therapy by targeting metabolic pathways.
