Four extracts of the marine-derived fungus Penicillium velutinum J.F.H.Beyma were obtained via metal ions stress conditions based on the OSMAC(One Strain Many Compounds)strategy.Using a combination of modern approache...Four extracts of the marine-derived fungus Penicillium velutinum J.F.H.Beyma were obtained via metal ions stress conditions based on the OSMAC(One Strain Many Compounds)strategy.Using a combination of modern approaches such as LC/UV,LC/MS and bioactivity data analysis,as well as in silico calculations,influence metal stress factors to change metabolite profiles Penicillium velutinum were analyzed.From the ethyl acetate extract of the P.velutinum were isolated two new piperazine derivatives helvamides B(1)and C(2)together with known saroclazin A(3)(4S,5R,7S)-4,11-dihydroxy-guaia-1(2),9(10)-dien(4).Their structures were established based on spectroscopic methods.The absolute configuration of helvamide B(1)as 2R,5R was determined by a combination of the X-ray analysis and by time-dependent density functional theory(TD-DFT)calculations of electronic circular dichroism(ECD)spectra.The cytotoxic activity of the isolated compounds against human prostate cancer PC-3 and human embryonic kidney HEK-293 cells and growth inhibition activity against yeast-like fungi Candida albicans were assayed.展开更多
The marine holothurian-derived fungal strain KMM 4401 has been identified as Paragliomastix luzulae using 28S rDNA,ITS regions and the partial TEF1 gene sequences.The metabolite profile of the fungal culture was studi...The marine holothurian-derived fungal strain KMM 4401 has been identified as Paragliomastix luzulae using 28S rDNA,ITS regions and the partial TEF1 gene sequences.The metabolite profile of the fungal culture was studied by UPLC-MS technique.The strain KMM 4401 is a source of various virescenoside-type isopimarane glycosides suggested as chemotaxonomic feature for this fungal species.Also Px.luzulae KMM 4401 was proposed as possible source of new bioactive secondary metabolites especially antimicrobials.Moreover,the co-cultures of Px.luzulae KMM 4401 with another marine fungus Penicillium hispanicum KMM 4689 inoculated simultaneously or after two weeks were investigated by same way.It was shown,that P.hispanicum KMM 4689 suppressed the production of most of Px.luzulae KMM 4401 metabolites.On the other hand,the co-cultivation of P.hispanicum KMM 4689 and Px.luzulae KMM 4401 resulted in increasing of production of main deoxyisoaustamide alkaloids of P.hispanicum KMM 4689 on 50-190%.展开更多
基金funded by Russian Science Foundation,grant number№22-73-00190,https://rscf.ru/en/project/22-73-00190/.
文摘Four extracts of the marine-derived fungus Penicillium velutinum J.F.H.Beyma were obtained via metal ions stress conditions based on the OSMAC(One Strain Many Compounds)strategy.Using a combination of modern approaches such as LC/UV,LC/MS and bioactivity data analysis,as well as in silico calculations,influence metal stress factors to change metabolite profiles Penicillium velutinum were analyzed.From the ethyl acetate extract of the P.velutinum were isolated two new piperazine derivatives helvamides B(1)and C(2)together with known saroclazin A(3)(4S,5R,7S)-4,11-dihydroxy-guaia-1(2),9(10)-dien(4).Their structures were established based on spectroscopic methods.The absolute configuration of helvamide B(1)as 2R,5R was determined by a combination of the X-ray analysis and by time-dependent density functional theory(TD-DFT)calculations of electronic circular dichroism(ECD)spectra.The cytotoxic activity of the isolated compounds against human prostate cancer PC-3 and human embryonic kidney HEK-293 cells and growth inhibition activity against yeast-like fungi Candida albicans were assayed.
基金funded by a grant from the Ministry of Science and Higher Education of the Russian Federation,15.BRK.21.0004(Contract No.075-15-2021-1052).
文摘The marine holothurian-derived fungal strain KMM 4401 has been identified as Paragliomastix luzulae using 28S rDNA,ITS regions and the partial TEF1 gene sequences.The metabolite profile of the fungal culture was studied by UPLC-MS technique.The strain KMM 4401 is a source of various virescenoside-type isopimarane glycosides suggested as chemotaxonomic feature for this fungal species.Also Px.luzulae KMM 4401 was proposed as possible source of new bioactive secondary metabolites especially antimicrobials.Moreover,the co-cultures of Px.luzulae KMM 4401 with another marine fungus Penicillium hispanicum KMM 4689 inoculated simultaneously or after two weeks were investigated by same way.It was shown,that P.hispanicum KMM 4689 suppressed the production of most of Px.luzulae KMM 4401 metabolites.On the other hand,the co-cultivation of P.hispanicum KMM 4689 and Px.luzulae KMM 4401 resulted in increasing of production of main deoxyisoaustamide alkaloids of P.hispanicum KMM 4689 on 50-190%.
