To promote sustainable aquaculture,plant-based ingredients are increasingly replacing fish meal(FM)and fish oil(FO)in aquafeeds,altering broodstock diets and reducing omega-3 long-chain polyunsaturated fatty acids(ω-...To promote sustainable aquaculture,plant-based ingredients are increasingly replacing fish meal(FM)and fish oil(FO)in aquafeeds,altering broodstock diets and reducing omega-3 long-chain polyunsaturated fatty acids(ω-3 LC-PUFAs),essential for reproductive success and progeny growth.Despite the critical role ofω-3 LC-PUFAs,particularly docosahexaenoic acid(DHA)in brain function,data on how fry cope with FM/FO-free diets during early development remain limited.To address this,we conducted a 2-year experiment comparing three broodstock diets:a commercial diet(C diet),a total plant-based diet(V1 diet),and a plant-based diet supplemented with DHA-rich Schizochytrium sp.microalgae oil(V2 diet).After reproduc-tion,progeny were fed either a C diet or a plant-based diet(V).Six groups(C-C,C-V,V1-C,V1-V,V2-C,V2-V)were analyzed for survival,feed intake,and growth,as well as neuropeptide,neurotransmitter,and intestinal hormone expres-sion.Results showed enhanced robustness in fry-fed V diets,particularly from V1 and V2-fed broodstock,with improved survival and feed intake.Fry from DHA-supplemented broodstock(V2-V)compensated for initial growth delays,achieving growth comparable to fry from commercial-fed mothers(C-V)within 30 days.Neurophysiological and gut-brain adapta-tions revealed complex compensatory mechanisms enabling fish to thrive on sustainable diets.These findings highlight the potential of DHA supplementation in plant-based diets to support sustainable aquaculture and warrant further validation under diverse nutritional and environmental conditions.展开更多
基金funded by the French National Research Agency(grant no.ANR-21-CE20-0009-01“FEEDOMEGA”).
文摘To promote sustainable aquaculture,plant-based ingredients are increasingly replacing fish meal(FM)and fish oil(FO)in aquafeeds,altering broodstock diets and reducing omega-3 long-chain polyunsaturated fatty acids(ω-3 LC-PUFAs),essential for reproductive success and progeny growth.Despite the critical role ofω-3 LC-PUFAs,particularly docosahexaenoic acid(DHA)in brain function,data on how fry cope with FM/FO-free diets during early development remain limited.To address this,we conducted a 2-year experiment comparing three broodstock diets:a commercial diet(C diet),a total plant-based diet(V1 diet),and a plant-based diet supplemented with DHA-rich Schizochytrium sp.microalgae oil(V2 diet).After reproduc-tion,progeny were fed either a C diet or a plant-based diet(V).Six groups(C-C,C-V,V1-C,V1-V,V2-C,V2-V)were analyzed for survival,feed intake,and growth,as well as neuropeptide,neurotransmitter,and intestinal hormone expres-sion.Results showed enhanced robustness in fry-fed V diets,particularly from V1 and V2-fed broodstock,with improved survival and feed intake.Fry from DHA-supplemented broodstock(V2-V)compensated for initial growth delays,achieving growth comparable to fry from commercial-fed mothers(C-V)within 30 days.Neurophysiological and gut-brain adapta-tions revealed complex compensatory mechanisms enabling fish to thrive on sustainable diets.These findings highlight the potential of DHA supplementation in plant-based diets to support sustainable aquaculture and warrant further validation under diverse nutritional and environmental conditions.
