Chronic diabetic wounds confront a significant medical challenge because of increasing prevalence and difficult-healing circumstances.It is vital to develop multifunctional hydrogel dressings,with well-designed morpho...Chronic diabetic wounds confront a significant medical challenge because of increasing prevalence and difficult-healing circumstances.It is vital to develop multifunctional hydrogel dressings,with well-designed morphology and structure to enhance flexibility and effectiveness in wound management.To achieve these,we propose a self-healing hydrogel dressing based on structural color microspheres for wound management.The microsphere comprised a photothermal-responsive inverse opal framework,which was constructed by hyaluronic acid methacryloyl,silk fibroin methacryloyl and black phosphorus quantum dots(BPQDs),and was further re-filled with a dynamic hydrogel.The dynamic hydrogel filler was formed by Knoevenagel condensation reaction between cyanoacetate and benzaldehyde-functionalized dextran(DEX-CA and DEX-BA).Notably,the composite microspheres can be applied arbitrarily,and they can adhere together upon near-infrared irradiation by leveraging the BPQDs-mediated photothermal effect and the thermoreversible stiffness change of dynamic hydrogel.Additionally,eumenitin and vascular endothelial growth factor were co-loaded in the microspheres and their release behavior can be regulated by the same mechanism.Moreover,effective monitoring of the drug release process can be achieved through visual color variations.The microsphere system has demonstrated desired capabilities of controllable drug release and efficient wound management.These characteristics suggest broad prospects for the proposed composite microspheres in clinical applications.展开更多
Hydrogel-based patches have demonstrated their values in diabetic wounds repair,particularly those intelligent dressings with continuous repair promoting and monitoring capabilities.Here,we propose a type of dual phys...Hydrogel-based patches have demonstrated their values in diabetic wounds repair,particularly those intelligent dressings with continuous repair promoting and monitoring capabilities.Here,we propose a type of dual physiological responsive structural color particles for wound repair.The particles are composed of a hyaluronic acid methacryloyl(HAMA)-sodium alginate(Alg)inverse opal scaffold,filled with oxidized dextran(ODex)/quaternized chitosan(QCS)hydrogel.The photo-polymerized HAMA and ionically cross-linked Ca-Alg constitute to the dual-network hydrogel with stable structural color.Furthermore,the ODex/QCS hydrogel,combined with glucose oxidase(GOX),exhibits pH/glucose dual responsiveness.Moreover,antimmicrobial peptide(AMP)plus vascular endothelial growth factor(VEGF)are comprised within the GOX-doped ODex/QCS hydrogel.In the high-glucose wound environment,GOX catalyzes glucose to generate acidic products,triggering rapid release of AMP and VEGF.Importantly,this process also leads to structural color changes of the particles,offering significant potential for wound monitoring.It has been demonstrated that such particles greatly promote the healing progress of diabetic wound in vivo.These results indicate that the present dual responsive particles would find valuable applications in diabetic wounds repair and the associated areas.展开更多
Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions.Stem cells have gained attention in anti-photoaging,while controlling the t...Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions.Stem cells have gained attention in anti-photoaging,while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulating cell fate and achieving optimal therapeutic performances.Here,we introduce a mechanically regulated human recombinant collagen(RHC)microcarrier generated through microfluidics,which is capable of modulating stem cell differentiation to treat photoaged skin.By controlling the cross-linking parameters,the mechanical properties of microcarriers could precisely tuned to optimize the stem cell differentiation.The microcarriers are surface functionalized with fibronectin(Fn)-platelet derived growth factor-BB(PDGF-BB)to facilitate adipose derived mesenchymal stem cells(Ad-MSCs)loading.In in vivo experiments,subcutaneous injection of stem cell loaded RHC microcarriers significantly reduced skin wrinkles after ultraviolet-injury,effectively promoted collagen synthesis,and increased vascular density.These encouraging results indicate that the present mechanically regulated microcarriers have great potential to deliver stem cells and regulate their differentiation for anti-photoaging treatments.展开更多
The development of tumor drug microcarriers has attracted considerable interest due to their distinctive therapeutic performances.Current attempts tend to elab-orate on the micro/nano-structure design of the microcarr...The development of tumor drug microcarriers has attracted considerable interest due to their distinctive therapeutic performances.Current attempts tend to elab-orate on the micro/nano-structure design of the microcarriers to achieve multiple drug delivery and spatiotemporal responsive features.Here,the desired hydrogel microspheres are presented with spatiotemporal responsiveness for the treatment of gastric cancer.The microspheres are generated based on inverse opals,their skele-ton is fabricated by biofriendly hyaluronic acid methacrylate(HAMA)and gelatin methacrylate(GelMA),and is thenfilled with a phase-changing hydrogel composed offish gelatin and agarose.Besides,the incorporated black phosphorus quantum dots(BPQDs)within thefilling hydrogel endow the microspheres with outstanding pho-tothermal responsiveness.Two antitumor drugs,sorafenib(SOR)and doxorubicin(DOX),are loaded in the skeleton andfilling hydrogel,respectively.It is found that the drugs show different release profiles upon near-infrared(NIR)irradiation,which exerts distinct performances in a controlled manner.Through both in vitro and in vivo experiments,it is demonstrated that such microspheres can significantly reduce tumor cell viability and enhance the efficiency in treating gastric cancer,indicating a promising stratagem in thefield of drug delivery and tumor therapy.展开更多
基金supported by the Ruijin Hospital Guangci Introducing Talent Projectfinancial support from National Natural Science Foundation of China(82372145)+4 种基金the Research Fellow(Grant No.353146)Research Project(347897)Solutions for Health Profile(336355)InFLAMES Flagship(337531)grants from Academy of Finlandthe Finland China Food and Health International Pilot Project funded by the Finnish Ministry of Education and Culture.
文摘Chronic diabetic wounds confront a significant medical challenge because of increasing prevalence and difficult-healing circumstances.It is vital to develop multifunctional hydrogel dressings,with well-designed morphology and structure to enhance flexibility and effectiveness in wound management.To achieve these,we propose a self-healing hydrogel dressing based on structural color microspheres for wound management.The microsphere comprised a photothermal-responsive inverse opal framework,which was constructed by hyaluronic acid methacryloyl,silk fibroin methacryloyl and black phosphorus quantum dots(BPQDs),and was further re-filled with a dynamic hydrogel.The dynamic hydrogel filler was formed by Knoevenagel condensation reaction between cyanoacetate and benzaldehyde-functionalized dextran(DEX-CA and DEX-BA).Notably,the composite microspheres can be applied arbitrarily,and they can adhere together upon near-infrared irradiation by leveraging the BPQDs-mediated photothermal effect and the thermoreversible stiffness change of dynamic hydrogel.Additionally,eumenitin and vascular endothelial growth factor were co-loaded in the microspheres and their release behavior can be regulated by the same mechanism.Moreover,effective monitoring of the drug release process can be achieved through visual color variations.The microsphere system has demonstrated desired capabilities of controllable drug release and efficient wound management.These characteristics suggest broad prospects for the proposed composite microspheres in clinical applications.
基金supported by the National Key Research and Development Program of China(2022YFA1105300)the National Natural Science Foundation of China(82372145,82102181,52403189)+5 种基金the Natural Science Foundation of Jiangsu Province(BK20210009)the Nanjing Distinguished Young Scholars Foundation(JQX22002)supported by the Research Project(347897)Solution for Health Profile(336355)InFLAMES Flagship(337531)"Printed Intelligence Infrastructure(PII-FIRI)"from Research Council of Finland.
文摘Hydrogel-based patches have demonstrated their values in diabetic wounds repair,particularly those intelligent dressings with continuous repair promoting and monitoring capabilities.Here,we propose a type of dual physiological responsive structural color particles for wound repair.The particles are composed of a hyaluronic acid methacryloyl(HAMA)-sodium alginate(Alg)inverse opal scaffold,filled with oxidized dextran(ODex)/quaternized chitosan(QCS)hydrogel.The photo-polymerized HAMA and ionically cross-linked Ca-Alg constitute to the dual-network hydrogel with stable structural color.Furthermore,the ODex/QCS hydrogel,combined with glucose oxidase(GOX),exhibits pH/glucose dual responsiveness.Moreover,antimmicrobial peptide(AMP)plus vascular endothelial growth factor(VEGF)are comprised within the GOX-doped ODex/QCS hydrogel.In the high-glucose wound environment,GOX catalyzes glucose to generate acidic products,triggering rapid release of AMP and VEGF.Importantly,this process also leads to structural color changes of the particles,offering significant potential for wound monitoring.It has been demonstrated that such particles greatly promote the healing progress of diabetic wound in vivo.These results indicate that the present dual responsive particles would find valuable applications in diabetic wounds repair and the associated areas.
基金supported by the National Key Research and Development Program of China(2022YFA1105300)the National Natural Science Foundation of China(52073060,61927805 and 82400718)+5 种基金the Nanjing Medical Science and Technique Development Foundation(ZKX21019)the Clinical Trials from Nanjing Drum Tower Hospital(2022-LCYJ-ZD-01)supported by the Research Project(347897)Solution for Health Profile(336355)InFLAMES Flagship(337531)"Printed Intelligence Infrastructure"(PII-FIRI)"from Research Council of Finland.
文摘Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions.Stem cells have gained attention in anti-photoaging,while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulating cell fate and achieving optimal therapeutic performances.Here,we introduce a mechanically regulated human recombinant collagen(RHC)microcarrier generated through microfluidics,which is capable of modulating stem cell differentiation to treat photoaged skin.By controlling the cross-linking parameters,the mechanical properties of microcarriers could precisely tuned to optimize the stem cell differentiation.The microcarriers are surface functionalized with fibronectin(Fn)-platelet derived growth factor-BB(PDGF-BB)to facilitate adipose derived mesenchymal stem cells(Ad-MSCs)loading.In in vivo experiments,subcutaneous injection of stem cell loaded RHC microcarriers significantly reduced skin wrinkles after ultraviolet-injury,effectively promoted collagen synthesis,and increased vascular density.These encouraging results indicate that the present mechanically regulated microcarriers have great potential to deliver stem cells and regulate their differentiation for anti-photoaging treatments.
基金National Natural Science Foundation of China,Grant/Award Number:82372145Research Fellow,Grant/Award Number:353146+3 种基金Research Project,Grant/Award Number:347897Solutions for Health Profile,Grant/Award Number:336355InFLAMES Flagship,Grant/Award Number:337531Finland China Food and Health International Pilot project funded by Finnish MInistry of Education and Culture。
文摘The development of tumor drug microcarriers has attracted considerable interest due to their distinctive therapeutic performances.Current attempts tend to elab-orate on the micro/nano-structure design of the microcarriers to achieve multiple drug delivery and spatiotemporal responsive features.Here,the desired hydrogel microspheres are presented with spatiotemporal responsiveness for the treatment of gastric cancer.The microspheres are generated based on inverse opals,their skele-ton is fabricated by biofriendly hyaluronic acid methacrylate(HAMA)and gelatin methacrylate(GelMA),and is thenfilled with a phase-changing hydrogel composed offish gelatin and agarose.Besides,the incorporated black phosphorus quantum dots(BPQDs)within thefilling hydrogel endow the microspheres with outstanding pho-tothermal responsiveness.Two antitumor drugs,sorafenib(SOR)and doxorubicin(DOX),are loaded in the skeleton andfilling hydrogel,respectively.It is found that the drugs show different release profiles upon near-infrared(NIR)irradiation,which exerts distinct performances in a controlled manner.Through both in vitro and in vivo experiments,it is demonstrated that such microspheres can significantly reduce tumor cell viability and enhance the efficiency in treating gastric cancer,indicating a promising stratagem in thefield of drug delivery and tumor therapy.
