Sugar beet molasses,a sugar-rich byproduct of sugar beet industry,have great potential as a biorefinery feedstock for the production of value-added bio-products by microbial fermentation.The production of functional e...Sugar beet molasses,a sugar-rich byproduct of sugar beet industry,have great potential as a biorefinery feedstock for the production of value-added bio-products by microbial fermentation.The production of functional exopolysaccharides(EPS)by lactic acid bacteria(LAB)represents a promising strategy for valorizing sugar processing waste.This study screened and identified high-efficiency EPS-producing LAB isolates from beet juice.Among the 32 isolates,six LAB strains including Limosilactobacillus fermentum YL7,Lactiplantibacillus plantarum YL4,TC10,and CJ10,Leuconostoc mesenteroides TCT3 and TCT4,exhibited efficient bio-conversion of beet molasses to LAB-EPS,with yields varying from 13.96 to 22.26 g/L.Antioxidant activity analysis revealed that EPS from these strains displayed significant scavenging activities against ABTS^(+),DPPH,and-OH radicals.Notably,EPS produced by L.plantarum TC10 and L.fermentum YL7 exhibited superior ABTS+scavenging capacity,with rates up to 99.48% and 95.89% at 4 mg/mL,respectively.Additionally,EPS from strains YL4,YL7,TCT3 and TCT4 showed significant antibiofilm activity against S.aureus and E.coli,with EPS-YL7 inhibiting S.aureus biofilm formation by up to 90.49% at 8.0 mg/mL.Furthermore,EPS from strains TCT4 demonstrated great tolerance to digestive fluid compared to EPS from other strains,with a retention rate of 72% at the end of intestinal digestion.These findings suggest that EPS-producing LAB strains from beet juice can efficiently convert beet molasses into valuable EPS utilized as functional food ingredients.展开更多
This study aimed to determine the efficacy of D-limonene in preventing biofilm formation by Bacillus amyloli-quefaciens,a soy food spoilage bacterium.The minimal biofilm inhibitory concentrations(MBICs)of D-limonene a...This study aimed to determine the efficacy of D-limonene in preventing biofilm formation by Bacillus amyloli-quefaciens,a soy food spoilage bacterium.The minimal biofilm inhibitory concentrations(MBICs)of D-limonene against two strains of B.amyloliquefaciens were determined using a twofold dilution method.Furthermore,the ability of D-limonene to eradicate preformed biofilms was evaluated using the TTC reduction method.Subsequent analyses investigated the effects of D-limonene on biofilm morphology,cell motility,cell surface characteristics,and the composition of the biofilm matrix.The MBICs of D-limonene for B.amyloliquefaciens strains DY1a and DY1b were determined to be 24 and 36μL/mL,respectively.At 2 MBIC,D-limonene eradicated 63.67%of the DY1a biofilms and 52.63%of the DY1b biofilms.Significantly,D-limonene markedly reduced swimming and swarming motility at concentrations ranging from 1/2 to 1 MBIC.In particular,at 1/2 MBIC,D-limonene decreased swimming and swarming abilities by 85.88%and 37.84%,respectively,for strain DY1a and by 75.00%and 95.07%,respectively,for strain DY1b.Furthermore,D-limonene suppressed the biosynthesis of poly-saccharides and proteins in the extracellular biofilm matrix and reduced cell surface hydrophobicity,surface charge,and autoaggregation.These findings suggest that D-limonene could be an effective antibiofilm agent for the control of B.amyloliquefaciens in the soybean food industry.展开更多
The present study sought to explore the digestion and fermentation properties of chayote pectin(CP)and its impact on human gut microbiota through simulated digestion and fecal fermentation.Results revealed that the di...The present study sought to explore the digestion and fermentation properties of chayote pectin(CP)and its impact on human gut microbiota through simulated digestion and fecal fermentation.Results revealed that the digestibility of CP in saliva,saliva-gastric liquid,and saliva-gastrointestinal digestion was 1.33%,4.61%,and 11.98%,respectively.Significant changes in the physicochemical properties of CP were observed during the simulated digestion process,such as total uronic acid,total protein,total phenols,reducing sugar,and molecular weight(Mw).The reducing sugar increased from 1.27×10^(-2) mg/mL to 140.16×10^(-2) mg/mL,and the Mw decreased from 4.00×10^(5) Da to 3.64×10^(5) Da.The Mw was reduced,which was primarily due to aggregate disruption during saliva digestion.However,during saliva-gastric and saliva-gastrointestinal digestions,the Mw was reduced due to aggregate disruption and glycosidic bond cleavage.The results indicate that CP was partially degraded during in vitro digestion.In addition,CP retained its antioxidant activity,binding capacity,and prebiotic activity during digestion.Additionally,the relative abundance of Bacteroides,Klebsiella,and Bifidobacterium exhibited a substantial increase,suggesting that the saliva-gastrointestinal digested sample of CP(CP-I)could alter the composition and abundance of human gut microbiota.Moreover,CP-I could promote the production of SCFAs during fermentation for 48 h.Especially,acetic acid increased from 44.45 to 1029.53μg/mL.The results indicate that CP-I was utilized by human gut microbiota.Overall,the results can provide valuable scientific basis for CP as a functional food ingredient in the future.展开更多
基金supported by Bofeng Talent Plan Project of Xinjiang Changji Hui Autonomous Prefecture,China(2021015)Students’Research Interesting Training Support by Sichuan Agricultural University,China(2023243).
文摘Sugar beet molasses,a sugar-rich byproduct of sugar beet industry,have great potential as a biorefinery feedstock for the production of value-added bio-products by microbial fermentation.The production of functional exopolysaccharides(EPS)by lactic acid bacteria(LAB)represents a promising strategy for valorizing sugar processing waste.This study screened and identified high-efficiency EPS-producing LAB isolates from beet juice.Among the 32 isolates,six LAB strains including Limosilactobacillus fermentum YL7,Lactiplantibacillus plantarum YL4,TC10,and CJ10,Leuconostoc mesenteroides TCT3 and TCT4,exhibited efficient bio-conversion of beet molasses to LAB-EPS,with yields varying from 13.96 to 22.26 g/L.Antioxidant activity analysis revealed that EPS from these strains displayed significant scavenging activities against ABTS^(+),DPPH,and-OH radicals.Notably,EPS produced by L.plantarum TC10 and L.fermentum YL7 exhibited superior ABTS+scavenging capacity,with rates up to 99.48% and 95.89% at 4 mg/mL,respectively.Additionally,EPS from strains YL4,YL7,TCT3 and TCT4 showed significant antibiofilm activity against S.aureus and E.coli,with EPS-YL7 inhibiting S.aureus biofilm formation by up to 90.49% at 8.0 mg/mL.Furthermore,EPS from strains TCT4 demonstrated great tolerance to digestive fluid compared to EPS from other strains,with a retention rate of 72% at the end of intestinal digestion.These findings suggest that EPS-producing LAB strains from beet juice can efficiently convert beet molasses into valuable EPS utilized as functional food ingredients.
基金supported by the Project of Science and Technology Innovation Center of National Modern Agricultural Industry(Ya’an),China(kczx2023-2025-07)Sichuan Province Science and Technology Support Program,China(2022ZHCG0064).
文摘This study aimed to determine the efficacy of D-limonene in preventing biofilm formation by Bacillus amyloli-quefaciens,a soy food spoilage bacterium.The minimal biofilm inhibitory concentrations(MBICs)of D-limonene against two strains of B.amyloliquefaciens were determined using a twofold dilution method.Furthermore,the ability of D-limonene to eradicate preformed biofilms was evaluated using the TTC reduction method.Subsequent analyses investigated the effects of D-limonene on biofilm morphology,cell motility,cell surface characteristics,and the composition of the biofilm matrix.The MBICs of D-limonene for B.amyloliquefaciens strains DY1a and DY1b were determined to be 24 and 36μL/mL,respectively.At 2 MBIC,D-limonene eradicated 63.67%of the DY1a biofilms and 52.63%of the DY1b biofilms.Significantly,D-limonene markedly reduced swimming and swarming motility at concentrations ranging from 1/2 to 1 MBIC.In particular,at 1/2 MBIC,D-limonene decreased swimming and swarming abilities by 85.88%and 37.84%,respectively,for strain DY1a and by 75.00%and 95.07%,respectively,for strain DY1b.Furthermore,D-limonene suppressed the biosynthesis of poly-saccharides and proteins in the extracellular biofilm matrix and reduced cell surface hydrophobicity,surface charge,and autoaggregation.These findings suggest that D-limonene could be an effective antibiofilm agent for the control of B.amyloliquefaciens in the soybean food industry.
基金supported by the Innovation project of the key cooperative research and development project between Ya’an City and SICAU[23ZDYF0001]the National Modern Agriculture Industry Technology Innovation Center[KCZX2023-2025-06]the Natural Science Foundation of Sichuan Province[2024NSFSC1251].
文摘The present study sought to explore the digestion and fermentation properties of chayote pectin(CP)and its impact on human gut microbiota through simulated digestion and fecal fermentation.Results revealed that the digestibility of CP in saliva,saliva-gastric liquid,and saliva-gastrointestinal digestion was 1.33%,4.61%,and 11.98%,respectively.Significant changes in the physicochemical properties of CP were observed during the simulated digestion process,such as total uronic acid,total protein,total phenols,reducing sugar,and molecular weight(Mw).The reducing sugar increased from 1.27×10^(-2) mg/mL to 140.16×10^(-2) mg/mL,and the Mw decreased from 4.00×10^(5) Da to 3.64×10^(5) Da.The Mw was reduced,which was primarily due to aggregate disruption during saliva digestion.However,during saliva-gastric and saliva-gastrointestinal digestions,the Mw was reduced due to aggregate disruption and glycosidic bond cleavage.The results indicate that CP was partially degraded during in vitro digestion.In addition,CP retained its antioxidant activity,binding capacity,and prebiotic activity during digestion.Additionally,the relative abundance of Bacteroides,Klebsiella,and Bifidobacterium exhibited a substantial increase,suggesting that the saliva-gastrointestinal digested sample of CP(CP-I)could alter the composition and abundance of human gut microbiota.Moreover,CP-I could promote the production of SCFAs during fermentation for 48 h.Especially,acetic acid increased from 44.45 to 1029.53μg/mL.The results indicate that CP-I was utilized by human gut microbiota.Overall,the results can provide valuable scientific basis for CP as a functional food ingredient in the future.
