Background:In the present study prevalence of mastitis causing pathogens and their antimicrobial resistance was studied with an objective to identify most common causative agents as well as their antibiotic resistance...Background:In the present study prevalence of mastitis causing pathogens and their antimicrobial resistance was studied with an objective to identify most common causative agents as well as their antibiotic resistance.Methods:The study encompassed collection of 880 quarters milk samples from 231 animals from different dairy farms of Punjab.The samples were subjected to bacterial isolation and further confirmation by MALDI-TOF.The antibiotic sensitivity testing of isolates was also done.Results:Out of 880 milk samples,562(63.86%)were found positive by California Mastitis Test(CMT).On the basis of CMT,left hind quarters were found to be more affected 26.86%(151/222)followed by left forequarters 24.90%(140/219),right hind quarters 24.70%(139/222)and right forequarters 23.30%(13/217).Out of 562 quarter milk samples,347(61.74%)samples yielded bacterial growth and a total of 364 isolates were identified and confirmed by Matrix Assisted Laser Desorption Ionization-Time Of Flight(MALDI-TOF)analysis.It was observed that among the Gram positive organisms the prevalence of Staphylococcus spp.was(58.24%),followed by Streptococcus spp.(8.51%),Enterococcus spp.(2.74%),and Corynebacterium spp.(1.09%).Among the Gram negative organisms,most prevalent was Escherichia coli(17.58%),followed by Pseudomonas spp.(6.59%),Enterobacter spp.(2.47%),Acinetobacter spp.(1.92%)and Klebsiella spp.(0.84%).On the basis of antibiotic resistance,Oxacillin,Methicillin,Penicillin,ampicillin,chloramphenicol,co-trimoxazole,ampicillin/sulbactam,gentamicin and enrofloxacin were resistant antibiotics.Conclusion:There is high prevalence of mastitis and the most prevalent bacteria involved in the mastitis were coagulase negative staphylococci,Staphylococcus aureus and Escherichia coli.On the basis of antibiotic sensitivity test these bacteria were found resistant to various antibiotics which requires attention.展开更多
文摘Background:In the present study prevalence of mastitis causing pathogens and their antimicrobial resistance was studied with an objective to identify most common causative agents as well as their antibiotic resistance.Methods:The study encompassed collection of 880 quarters milk samples from 231 animals from different dairy farms of Punjab.The samples were subjected to bacterial isolation and further confirmation by MALDI-TOF.The antibiotic sensitivity testing of isolates was also done.Results:Out of 880 milk samples,562(63.86%)were found positive by California Mastitis Test(CMT).On the basis of CMT,left hind quarters were found to be more affected 26.86%(151/222)followed by left forequarters 24.90%(140/219),right hind quarters 24.70%(139/222)and right forequarters 23.30%(13/217).Out of 562 quarter milk samples,347(61.74%)samples yielded bacterial growth and a total of 364 isolates were identified and confirmed by Matrix Assisted Laser Desorption Ionization-Time Of Flight(MALDI-TOF)analysis.It was observed that among the Gram positive organisms the prevalence of Staphylococcus spp.was(58.24%),followed by Streptococcus spp.(8.51%),Enterococcus spp.(2.74%),and Corynebacterium spp.(1.09%).Among the Gram negative organisms,most prevalent was Escherichia coli(17.58%),followed by Pseudomonas spp.(6.59%),Enterobacter spp.(2.47%),Acinetobacter spp.(1.92%)and Klebsiella spp.(0.84%).On the basis of antibiotic resistance,Oxacillin,Methicillin,Penicillin,ampicillin,chloramphenicol,co-trimoxazole,ampicillin/sulbactam,gentamicin and enrofloxacin were resistant antibiotics.Conclusion:There is high prevalence of mastitis and the most prevalent bacteria involved in the mastitis were coagulase negative staphylococci,Staphylococcus aureus and Escherichia coli.On the basis of antibiotic sensitivity test these bacteria were found resistant to various antibiotics which requires attention.
