Currently,teas and herbal infusions represent an important part of the human diet.Most contain phenolic com-pounds with high antioxidant activity,usually associated with human health protective functions.This attribut...Currently,teas and herbal infusions represent an important part of the human diet.Most contain phenolic com-pounds with high antioxidant activity,usually associated with human health protective functions.This attribute defines teas and infusions as nutraceutical foods.In Argentina,several native species are traditionally used for medi-cal purposes.Some of those species are Larrea cuneifolia,Larrea nitida,Grindelia chiloensis,Pteromonnina dictyo-carpa,Mandevilla laxa,and Monttea aphylla.The objectives of this study were to analyze the antioxidant power and the scavenging capacity of infusions obtained from those six medicinal plants,to characterize the phenolic profile,and to study in vitro their safety or cytotoxicity.Additionally,the potential use of two infusions as antioxidant additives in a food model was evaluated.The results indicated that the analyzed plant species are rich in phenolic acids(e.g.,caffeic,ferulic,and chlorogenic acid),and flavonoids(e.g.,quercetin and kaempferol),with high antioxi-dant power.The infusion blend obtained with G.chiloensis and L.cuneifolia exhibited the highest value of antioxidant capacity measured with the FRAP technique(193.4μg EAA/mg DW).On the other hand,L.cuneifolia infusion showed the greatest antioxidant capacity determined by FRAP(131.9±5.2μg EAA/mg DW)and DPPH assays(0.453 mL/mg s).Additionally,L.cuneifolia infusion showed the highest phenolic content(232.8μg GAE/mg DW)and flavonoid content(153.3μg QE/mg DW).None of the infusions showed toxicity in mammalian cells,except for G.chiloensis.Furthermore,the L.cuneifolia and L.nitida infusions showed a high inhibitory effect on lipid oxidation in ground beef(55%and 51%at 4 days of storage,respectively).The results suggest that the studied infusions are safe and a rich source of antioxidants,which supports their use in traditional medicine.However,further exhaustive studies of G.chiloensis infusion are needed to ensure its safety,as it has shown cytotoxicity.Besides,it is worthwhile to advance the study of L.cuneifolia and L.nitida as sources of dietary antioxidants,due to their high antioxidant power and ability to protect against lipid peroxidation.展开更多
基金supported by the following grants:Universidad Nacional del Comahue(UNCo)(PIN 04/N037)Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)(PIP 2020-1243)Agencia Nacional de Promoción Científica y Tecnológica(ANPCyT)(PICT 2020-3049).
文摘Currently,teas and herbal infusions represent an important part of the human diet.Most contain phenolic com-pounds with high antioxidant activity,usually associated with human health protective functions.This attribute defines teas and infusions as nutraceutical foods.In Argentina,several native species are traditionally used for medi-cal purposes.Some of those species are Larrea cuneifolia,Larrea nitida,Grindelia chiloensis,Pteromonnina dictyo-carpa,Mandevilla laxa,and Monttea aphylla.The objectives of this study were to analyze the antioxidant power and the scavenging capacity of infusions obtained from those six medicinal plants,to characterize the phenolic profile,and to study in vitro their safety or cytotoxicity.Additionally,the potential use of two infusions as antioxidant additives in a food model was evaluated.The results indicated that the analyzed plant species are rich in phenolic acids(e.g.,caffeic,ferulic,and chlorogenic acid),and flavonoids(e.g.,quercetin and kaempferol),with high antioxi-dant power.The infusion blend obtained with G.chiloensis and L.cuneifolia exhibited the highest value of antioxidant capacity measured with the FRAP technique(193.4μg EAA/mg DW).On the other hand,L.cuneifolia infusion showed the greatest antioxidant capacity determined by FRAP(131.9±5.2μg EAA/mg DW)and DPPH assays(0.453 mL/mg s).Additionally,L.cuneifolia infusion showed the highest phenolic content(232.8μg GAE/mg DW)and flavonoid content(153.3μg QE/mg DW).None of the infusions showed toxicity in mammalian cells,except for G.chiloensis.Furthermore,the L.cuneifolia and L.nitida infusions showed a high inhibitory effect on lipid oxidation in ground beef(55%and 51%at 4 days of storage,respectively).The results suggest that the studied infusions are safe and a rich source of antioxidants,which supports their use in traditional medicine.However,further exhaustive studies of G.chiloensis infusion are needed to ensure its safety,as it has shown cytotoxicity.Besides,it is worthwhile to advance the study of L.cuneifolia and L.nitida as sources of dietary antioxidants,due to their high antioxidant power and ability to protect against lipid peroxidation.
