Objective:To evaluate antimicrobial and antimycobacterial activity of three seaweeds[Gelidiella acerosa(G.acerosa),Turbinaria conoides(T.conoides)and Sargassum wightii(S.wightii)]from Gulf of Manner.Methods:Three seaw...Objective:To evaluate antimicrobial and antimycobacterial activity of three seaweeds[Gelidiella acerosa(G.acerosa),Turbinaria conoides(T.conoides)and Sargassum wightii(S.wightii)]from Gulf of Manner.Methods:Three seaweeds G.acerosa,T.conoides and S.wightii were collected from Gulf of Mannar,South-East Coast of India.Solvent extraction of the selected seaweeds was done using hexane and ethanol.These extracts were tested for antibacterial activity against four bacterial strains(Escherichia coli,Pseudomonas aeruginosa,Bacillus subtilis and Staphylococcus aureus)by disc diffusion method.The active extracts of T.conoides were tested for antimycobacterial activity by luciferase reporter phage assay at two different concentrations(100μg/mL and 500μg/mL).Results:Among the tested seaweeds,ethanol extract of T.conoides showed maximum antibacterial activity against Bacillus subtilis and Staphylococcus aureus followed by hexane extract at concentration of 1 mg/disc.The remaining two seaweeds(G.acerosa and S.wightii)did not show any activity.The ethanol extract of T.conoides exhibited maximum antimycobacterial activity(87.33%)followed by hexane extract(74.68%)against Mycobacterium tuberculosis H37Rv at 500μg/mL.Conclusions:Ethanol extract of T.conoids showed both antibacterial and antimycobacterial activity.Further photochemical studies are needed to identify the active antimycobacterial agents.展开更多
文摘Objective:To evaluate antimicrobial and antimycobacterial activity of three seaweeds[Gelidiella acerosa(G.acerosa),Turbinaria conoides(T.conoides)and Sargassum wightii(S.wightii)]from Gulf of Manner.Methods:Three seaweeds G.acerosa,T.conoides and S.wightii were collected from Gulf of Mannar,South-East Coast of India.Solvent extraction of the selected seaweeds was done using hexane and ethanol.These extracts were tested for antibacterial activity against four bacterial strains(Escherichia coli,Pseudomonas aeruginosa,Bacillus subtilis and Staphylococcus aureus)by disc diffusion method.The active extracts of T.conoides were tested for antimycobacterial activity by luciferase reporter phage assay at two different concentrations(100μg/mL and 500μg/mL).Results:Among the tested seaweeds,ethanol extract of T.conoides showed maximum antibacterial activity against Bacillus subtilis and Staphylococcus aureus followed by hexane extract at concentration of 1 mg/disc.The remaining two seaweeds(G.acerosa and S.wightii)did not show any activity.The ethanol extract of T.conoides exhibited maximum antimycobacterial activity(87.33%)followed by hexane extract(74.68%)against Mycobacterium tuberculosis H37Rv at 500μg/mL.Conclusions:Ethanol extract of T.conoids showed both antibacterial and antimycobacterial activity.Further photochemical studies are needed to identify the active antimycobacterial agents.
