Induced pluripotent stem cells （iPSCs） are an attractive cell source for regenerative medicine through cell therapy or drug screening. But application of iPSCs in regenerative medicine requires rapid and accurate ch...Induced pluripotent stem cells （iPSCs） are an attractive cell source for regenerative medicine through cell therapy or drug screening. But application of iPSCs in regenerative medicine requires rapid and accurate charac- terization of iPSCs. Here, we demonstrate the detection of multiple antigens present in iPSC lysate using rapid, label- free surface plasmon resonance imaging （SPRi） assay. Validation of pluripotency is an important aspect of iPSC research. In this study, we fabricated antibody array against pluripotency biomarkers and found that our array suc- cessfully detect corresponding antigens in stem cell lysate. Each antibody recognized its specific antigens presented in iPSC lysates and a certain degree of variability was observed in comparison with other cell lysates. The results suggested that SPRi is a versatile technology feasible for the detection of multiple antigens presented in iPSC lysate. Further extension of this method may be applied in the characterization and high-throughput biomarker profiling of iPSCs.展开更多
Erratum to:Protein Cell 2012,3(2):84-88 DOI 10.1007/s13238-012-2012-y Due to a typesetting error,(~3-7×10^(13)mol/L)in the fifth line of the“TUS-TER microarray”subsection on the right column of page 85 should b...Erratum to:Protein Cell 2012,3(2):84-88 DOI 10.1007/s13238-012-2012-y Due to a typesetting error,(~3-7×10^(13)mol/L)in the fifth line of the“TUS-TER microarray”subsection on the right column of page 85 should be(~3-7×10^(−13)mol/L).展开更多
Recently,in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins.In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA...Recently,in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins.In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA or RNA.The advances in in situ protein microarrays are exemplified by the ease of cDNA cloning and cell free protein expression.These technologies can evaluate,validate and monitor protein in a cost effective manner and address the issue of a high quality protein supply to use in the array.Here we review the importance of recently employed methods:PISA(protein in situ array),DAPA(DNA array to protein array),NAPPA(nucleic acid programmable protein array)and TUSTER microarrays and the role of these methods in proteomics.展开更多
基金partly supported by the National Natural Science Foundation of China(31171381)the National Basic Research Program of China,2012CB966701the core facility of the Tsinghua-Peking Center for Life Sciences
文摘Induced pluripotent stem cells （iPSCs） are an attractive cell source for regenerative medicine through cell therapy or drug screening. But application of iPSCs in regenerative medicine requires rapid and accurate charac- terization of iPSCs. Here, we demonstrate the detection of multiple antigens present in iPSC lysate using rapid, label- free surface plasmon resonance imaging （SPRi） assay. Validation of pluripotency is an important aspect of iPSC research. In this study, we fabricated antibody array against pluripotency biomarkers and found that our array suc- cessfully detect corresponding antigens in stem cell lysate. Each antibody recognized its specific antigens presented in iPSC lysates and a certain degree of variability was observed in comparison with other cell lysates. The results suggested that SPRi is a versatile technology feasible for the detection of multiple antigens presented in iPSC lysate. Further extension of this method may be applied in the characterization and high-throughput biomarker profiling of iPSCs.
文摘Erratum to:Protein Cell 2012,3(2):84-88 DOI 10.1007/s13238-012-2012-y Due to a typesetting error,(~3-7×10^(13)mol/L)in the fifth line of the“TUS-TER microarray”subsection on the right column of page 85 should be(~3-7×10^(−13)mol/L).
文摘Recently,in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins.In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA or RNA.The advances in in situ protein microarrays are exemplified by the ease of cDNA cloning and cell free protein expression.These technologies can evaluate,validate and monitor protein in a cost effective manner and address the issue of a high quality protein supply to use in the array.Here we review the importance of recently employed methods:PISA(protein in situ array),DAPA(DNA array to protein array),NAPPA(nucleic acid programmable protein array)and TUSTER microarrays and the role of these methods in proteomics.
