Geometric and structural integrity often deteriorate in 3D printed cell-laden constructs over time due to cellular compaction and hydrogel shrinkage.This study introduces a new approach that synergizes the advantages ...Geometric and structural integrity often deteriorate in 3D printed cell-laden constructs over time due to cellular compaction and hydrogel shrinkage.This study introduces a new approach that synergizes the advantages of cell compatibility of biological hydrogels and mechanical stability of elastomeric polymers for structure fidelity maintenance upon stereolithography and extrusion 3D printing.Enabling this advance is the composite bioink,formulated by integrating elastomeric microparticles from poly(octamethylene maleate(anhydride)citrate)(POMaC)into biologically derived hydrogels(fibrin,gelatin methacryloyl(GelMA),and alginate).The composite bioink enhanced the elasticity and plasticity of the 3D printed constructs,effectively mitigating tissue compaction and swelling.It exhibited a low shear modulus and a rapid crosslinking time,along with a high ultimate compressive strength and resistance to deformation from cellular forces and physical handling;this was attributed to packing and stress dissipation of elastomeric particles,which was confirmed via mathematical modelling.Enhanced functional assembly and stability of human iPSC-derived cardiac tissues and primary vasculature proved the utility of the composite bioink in tissue engineering.In vivo implantation studies revealed that constructs containing POMaC particles exhibited improved resilience against host tissue stress,enhanced angiogenesis,and infiltration of pro-reparative macrophages.展开更多
The substantial economic impact of non-healing wounds,scarring,and burns stemming from skin injuries is evident,resulting in a financial burden on both patients and the healthcare system.This review paper provides an ...The substantial economic impact of non-healing wounds,scarring,and burns stemming from skin injuries is evident,resulting in a financial burden on both patients and the healthcare system.This review paper provides an overview of the skin’s vital role in guarding against various environmental challenges as the body’s largest protective organ and associated developments in biomaterials for wound healing.We first introduce the composition of skin tissue and the intricate processes of wound healing,with special attention to the crucial role of immunomodulation in both acute and chronic wounds.This highlights how the imbalance in the immune response,particularly in chronic wounds associated with underlying health conditions such as diabetes and immunosuppression,hinders normal healing stages.Then,this review distinguishes between traditional wound-healing strategies that create an optimal microenvironment and recent peptide-based biomaterials that modulate cellular processes and immune responses to facilitate wound closure.Additionally,we highlight the importance of considering the stages of wounds in the healing process.By integrating advanced materials engineering with an in-depth understanding of wound biology,this approach holds promise for reshaping the field of wound management and ultimately offering improved outcomes for patients with acute and chronic wounds.展开更多
文摘Geometric and structural integrity often deteriorate in 3D printed cell-laden constructs over time due to cellular compaction and hydrogel shrinkage.This study introduces a new approach that synergizes the advantages of cell compatibility of biological hydrogels and mechanical stability of elastomeric polymers for structure fidelity maintenance upon stereolithography and extrusion 3D printing.Enabling this advance is the composite bioink,formulated by integrating elastomeric microparticles from poly(octamethylene maleate(anhydride)citrate)(POMaC)into biologically derived hydrogels(fibrin,gelatin methacryloyl(GelMA),and alginate).The composite bioink enhanced the elasticity and plasticity of the 3D printed constructs,effectively mitigating tissue compaction and swelling.It exhibited a low shear modulus and a rapid crosslinking time,along with a high ultimate compressive strength and resistance to deformation from cellular forces and physical handling;this was attributed to packing and stress dissipation of elastomeric particles,which was confirmed via mathematical modelling.Enhanced functional assembly and stability of human iPSC-derived cardiac tissues and primary vasculature proved the utility of the composite bioink in tissue engineering.In vivo implantation studies revealed that constructs containing POMaC particles exhibited improved resilience against host tissue stress,enhanced angiogenesis,and infiltration of pro-reparative macrophages.
基金the Canadian Institutes of Health Research(CIHR)Foundation Grant FDN-167274Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery Grant(RGPIN 326982-10)Stem Cell Network Impact Award 920530 and National Institutes of Health Grant 2R01 HL076485.
文摘The substantial economic impact of non-healing wounds,scarring,and burns stemming from skin injuries is evident,resulting in a financial burden on both patients and the healthcare system.This review paper provides an overview of the skin’s vital role in guarding against various environmental challenges as the body’s largest protective organ and associated developments in biomaterials for wound healing.We first introduce the composition of skin tissue and the intricate processes of wound healing,with special attention to the crucial role of immunomodulation in both acute and chronic wounds.This highlights how the imbalance in the immune response,particularly in chronic wounds associated with underlying health conditions such as diabetes and immunosuppression,hinders normal healing stages.Then,this review distinguishes between traditional wound-healing strategies that create an optimal microenvironment and recent peptide-based biomaterials that modulate cellular processes and immune responses to facilitate wound closure.Additionally,we highlight the importance of considering the stages of wounds in the healing process.By integrating advanced materials engineering with an in-depth understanding of wound biology,this approach holds promise for reshaping the field of wound management and ultimately offering improved outcomes for patients with acute and chronic wounds.
