AIM： TO investigate the ABH and Lewis antigen expression in erythrocytes, saliva and gastric epithelium, as well as the association between H pylori and the presence of gastric epithelial lesions.METHODS： The distri...AIM： TO investigate the ABH and Lewis antigen expression in erythrocytes, saliva and gastric epithelium, as well as the association between H pylori and the presence of gastric epithelial lesions.METHODS： The distribution of ABH and Lewis blood group antigens in erythrocytes, saliva and gastric mucosa of Hpylori-infected gastric ulcer patients was analyzed. Forty-two patients with gastric ulcer were studied, and fifty healthy individuals were used as control group. The blood group antigens were determined by direct hemagglutination, dot-ELISA and immunohistochemi- cal methods in erythrocytes, saliva and gastric mucosa specimens, respectively. Diagnosis for H pylori infection was performed by conventional optical microscopy and ELISA.RESULTS： A higher seroprevalence of IgG H pylori specific antibodies was observed in gastric ulcer patients （90%） compared to the control group （60%）. We observed a significant increase of phenotypes O, A2 and Lewis b in H pylori-infected patients. The expression of these antigens had progressive alterations in areas of ulcerous lesions and intestinal metaplasia.CONCLUSION： ABH and Lewis blood group antigens are a good indicator for cellular alterations in the gastric epithelium.展开更多
基金Supported by the Secretaria Executiva de Ci ncia,Tecnologia e Meio Ambiente-SECTAM Coordena o de Aperfei oamento de Pessoal de Nivel Superior-CAPES.Co-first-authors:Luisa Caricio Martins,Tereza Cristina de Oliveira Corvelo
文摘AIM： TO investigate the ABH and Lewis antigen expression in erythrocytes, saliva and gastric epithelium, as well as the association between H pylori and the presence of gastric epithelial lesions.METHODS： The distribution of ABH and Lewis blood group antigens in erythrocytes, saliva and gastric mucosa of Hpylori-infected gastric ulcer patients was analyzed. Forty-two patients with gastric ulcer were studied, and fifty healthy individuals were used as control group. The blood group antigens were determined by direct hemagglutination, dot-ELISA and immunohistochemi- cal methods in erythrocytes, saliva and gastric mucosa specimens, respectively. Diagnosis for H pylori infection was performed by conventional optical microscopy and ELISA.RESULTS： A higher seroprevalence of IgG H pylori specific antibodies was observed in gastric ulcer patients （90%） compared to the control group （60%）. We observed a significant increase of phenotypes O, A2 and Lewis b in H pylori-infected patients. The expression of these antigens had progressive alterations in areas of ulcerous lesions and intestinal metaplasia.CONCLUSION： ABH and Lewis blood group antigens are a good indicator for cellular alterations in the gastric epithelium.
