Cocoa bean shell(CBS),a by-product of the chocolate industry,was employed as substrate for the sustainable recovery of bioactive compounds using ohmic heating(OH).Total phenolic content and antioxidant activity of the...Cocoa bean shell(CBS),a by-product of the chocolate industry,was employed as substrate for the sustainable recovery of bioactive compounds using ohmic heating(OH).Total phenolic content and antioxidant activity of the treated CBS were optimized by experimental design.Maximum extraction of antioxidant phenolic compounds(23 mg GAE/g CBS)was obtained at 67℃,50 min and 44%ethanol(v/v).The antioxidant activity of the extracts obtained under of the central point conditions was 284.5μM Fe^(2+)/g extract(FRAP)and 36.4μM TE/g extract(DPPH).The use of OH increased the extraction of bioactive phenolic compounds when compared to the conventional process(CH)(approximately 40%).An increased on the chemical antioxidant activity was also observed,ranging from 4 to 20%.The metabolic activity of the extracts obtained by the two methods(OH and CH)was evaluated in non-tumoral(HEK293T and L929)and tumoral cell lines(Caco-2,HT-29,and HeLa).The CBS extracts presented low toxicity in non-tumoral cells and ROS preventive effects.These characteristics make them ideal to be used in food processing and formulation,as well as nutraceutical products due to their antioxidant protection.The use of OH results in an extract with higher phenolic content and higher antioxidant activity and low environmental impact.展开更多
基金supported by the Portuguese Foundation for Science and Technology(FCT)under the scope of both the strategic funding of UIDB/04469/2020 unit and LA/P/0029/2020(LABBELS-Associate Laboratory in Biotechnology,Bioengineering and Microelectromechanical Systems)by the Project SV-PA-21-AYUD/2021/51041 from the Economy and Employment Office of Principality of Asturias(Spain)J.S.G.-D.acknowledges the PhD scholarship 2021.06136.BD provided by FCT.Pedro Ferreira-Santos would like to express gratitude to the Spanish Ministry of Science,Innovation and Universities for financial support(FJC2021-046978-I).
文摘Cocoa bean shell(CBS),a by-product of the chocolate industry,was employed as substrate for the sustainable recovery of bioactive compounds using ohmic heating(OH).Total phenolic content and antioxidant activity of the treated CBS were optimized by experimental design.Maximum extraction of antioxidant phenolic compounds(23 mg GAE/g CBS)was obtained at 67℃,50 min and 44%ethanol(v/v).The antioxidant activity of the extracts obtained under of the central point conditions was 284.5μM Fe^(2+)/g extract(FRAP)and 36.4μM TE/g extract(DPPH).The use of OH increased the extraction of bioactive phenolic compounds when compared to the conventional process(CH)(approximately 40%).An increased on the chemical antioxidant activity was also observed,ranging from 4 to 20%.The metabolic activity of the extracts obtained by the two methods(OH and CH)was evaluated in non-tumoral(HEK293T and L929)and tumoral cell lines(Caco-2,HT-29,and HeLa).The CBS extracts presented low toxicity in non-tumoral cells and ROS preventive effects.These characteristics make them ideal to be used in food processing and formulation,as well as nutraceutical products due to their antioxidant protection.The use of OH results in an extract with higher phenolic content and higher antioxidant activity and low environmental impact.
