Corneal diseases,the second leading cause of global vision loss affecting over 10.5 million people,underscores the unmet demand for corneal tissue replacements.Given the scarcity of fresh donor corneas and the associa...Corneal diseases,the second leading cause of global vision loss affecting over 10.5 million people,underscores the unmet demand for corneal tissue replacements.Given the scarcity of fresh donor corneas and the associated risks of immune rejection,corneal tissue engineering becomes imperative.Developing nanofibrous scaffolds that mimic the natural corneal structure is crucial for creating transparent and mechanically robust corneal equivalents in tissue engineering.Herein,Aloe Vera Extract(AVE)/Polycaprolactone(PCL)nanofibrous scaffolds were primed using electrospinning.The electrospun AVE/PCL fibers exhibit a smooth,bead-free morphology with a mean diameter of approximately 340±95 nm and appropriate light transparency.Mechanical measurements reveal Young’s modulus and ultimate tensile strength values of around 3.34 MPa and 4.58 MPa,respectively,within the range of stromal tissue.In addition,cell viability of AVE/PCL fibers was measured against Human Stromal Keratocyte Cells(HSKCs),and improved cell viability was observed.The cell-fiber interactions were investigated using scanning electron microscopy.In conclusion,the incorporation of Aloe Vera Extract enhances the mechanical,optical,hydrophilic,and biological properties of PCL fibers,positioning PCL/AVE fiber scaffolds as promising candidates for corneal stromal regeneration.展开更多
AIM:To compare the visual results of non-topographyguided and topography-guided photorefractive keratectomy(PRK)applying sequential and simultaneous corneal crosslinking(CXL)treatment for keratoconus.METHODS:Intervent...AIM:To compare the visual results of non-topographyguided and topography-guided photorefractive keratectomy(PRK)applying sequential and simultaneous corneal crosslinking(CXL)treatment for keratoconus.METHODS:Interventional and comparative prospective study.Sixty-nine eyes(36 patients)suffering from keratoconus(stages 1 Amsler-Krumeich classification)were divided into four groups:sequential topography-guided photorefractive keratectomy with CXL,simultaneous topography-guided photorefractive keratectomy with CXL,simultaneous nontopography guided photorefractive keratectomy with CXL,and sequential non-topography guided photorefractive keratectomy with CXL.The main outcome measures were pre-and postoperative uncorrected distance visual acuity(UDVA),best corrected distance visual acuity(CDVA),manifest refraction,contrast sensitivity,and keratometry.RESULTS:All analyzed visual,contrast sensitivity,and refractive parameters showed a significant improvement in the four groups(all P<0.05).A noticeable improvement was seen in keratometry in all the groups,and a remarkable difference was observed between topography-guided groups in comparison to non-topography-guided groups(P<0.05).Interestingly,the improvement in all parameters showed a degree of stability to the end of the follow-up.CONCLUSION:The treatment priorities in all four groups are safety,efficacy,and predictability in the correction of the sphero-cylindrical errors in mild and moderate keratoconus.No significant differences among groups in the recorded objective outcomes were found.展开更多
基金supported by the Consejo Nacional de Ciencia y Tecnología(CONACyT)and Tecnológico de Monterreyreceived by CONACYT in the form of a Graduate Studies Scholarship。
文摘Corneal diseases,the second leading cause of global vision loss affecting over 10.5 million people,underscores the unmet demand for corneal tissue replacements.Given the scarcity of fresh donor corneas and the associated risks of immune rejection,corneal tissue engineering becomes imperative.Developing nanofibrous scaffolds that mimic the natural corneal structure is crucial for creating transparent and mechanically robust corneal equivalents in tissue engineering.Herein,Aloe Vera Extract(AVE)/Polycaprolactone(PCL)nanofibrous scaffolds were primed using electrospinning.The electrospun AVE/PCL fibers exhibit a smooth,bead-free morphology with a mean diameter of approximately 340±95 nm and appropriate light transparency.Mechanical measurements reveal Young’s modulus and ultimate tensile strength values of around 3.34 MPa and 4.58 MPa,respectively,within the range of stromal tissue.In addition,cell viability of AVE/PCL fibers was measured against Human Stromal Keratocyte Cells(HSKCs),and improved cell viability was observed.The cell-fiber interactions were investigated using scanning electron microscopy.In conclusion,the incorporation of Aloe Vera Extract enhances the mechanical,optical,hydrophilic,and biological properties of PCL fibers,positioning PCL/AVE fiber scaffolds as promising candidates for corneal stromal regeneration.
文摘AIM:To compare the visual results of non-topographyguided and topography-guided photorefractive keratectomy(PRK)applying sequential and simultaneous corneal crosslinking(CXL)treatment for keratoconus.METHODS:Interventional and comparative prospective study.Sixty-nine eyes(36 patients)suffering from keratoconus(stages 1 Amsler-Krumeich classification)were divided into four groups:sequential topography-guided photorefractive keratectomy with CXL,simultaneous topography-guided photorefractive keratectomy with CXL,simultaneous nontopography guided photorefractive keratectomy with CXL,and sequential non-topography guided photorefractive keratectomy with CXL.The main outcome measures were pre-and postoperative uncorrected distance visual acuity(UDVA),best corrected distance visual acuity(CDVA),manifest refraction,contrast sensitivity,and keratometry.RESULTS:All analyzed visual,contrast sensitivity,and refractive parameters showed a significant improvement in the four groups(all P<0.05).A noticeable improvement was seen in keratometry in all the groups,and a remarkable difference was observed between topography-guided groups in comparison to non-topography-guided groups(P<0.05).Interestingly,the improvement in all parameters showed a degree of stability to the end of the follow-up.CONCLUSION:The treatment priorities in all four groups are safety,efficacy,and predictability in the correction of the sphero-cylindrical errors in mild and moderate keratoconus.No significant differences among groups in the recorded objective outcomes were found.
