Objectives To determine how the anti-inflammatory actions of interleukin-10(IL-10)and IL-6 differ across age and physical activity levels.Methods Using a cross-sectional design,fasted blood samples were obtained from ...Objectives To determine how the anti-inflammatory actions of interleukin-10(IL-10)and IL-6 differ across age and physical activity levels.Methods Using a cross-sectional design,fasted blood samples were obtained from younger physically inactive(YI:n=10,age:22.7±3.7 years,BMI:24.8±4.8 kg/m^(2),<150 min of weekly moderate-to-vigorous physical activity[MVPA]),younger highly active(YA:n=11 varsity cross country running athletes,20.7±2.7 years,21.1±1.8 kg/m^(2),>300 min of weekly MVPA),and older highly active(OA:12,56.0±10.3 years,22.8±3.2 kg/m^(2),>300 min of weekly MVPA)individuals and analyzed for leukocyte counts,IL-10 and IL-6-related signaling,and cytokine secretion ex vivo.Results Total white blood cells and monocytes were similar between groups(p=0.8)but YA and OA had lower lymphocyte counts than YI(p<0.01).The ability of IL-10(1 ng/mL)to phosphorylate signal transducer and activator of transcription 3(STAT3)in CD14 monocytes was greater in YA vs.YI(p<0.03)despite YA having lower IL-10 receptor expression(p<0.01).IL-6(10 ng/mL)mediated STAT3 phosphorylation in CD4 lymphocytes was higher in OA compared YI(p<0.01),with a similar tendency observed for YA vs.YI(p=0.08).Despite enhanced responsiveness of STAT3 to IL-10/6 in active individuals,the ability of IL-10/6 to inhibit tumor necrosis factor-alpha(TNF-⍺)secretion from lipopolysaccharide-stimulated whole-blood was similar between groups.Conclusions Highly active younger and older individuals demonstrate enhanced IL-10-and IL-6-mediated activation of immune cell STAT3.Although the ability of IL-10/6 to inhibit TNF-⍺secretion appeared unimpacted by activity level,anti-inflammatory cytokine actions were preserved in older active individuals.展开更多
基金H.I.was supported by a Natural Sciences and Engineering Council(NSERC)postdoctoral fellowship and a Michael Smith Health Research BC Research Trainee AwardJ.B.was supported by and NSERC Undergraduate Student Research Award+2 种基金G.S.J.was supported by an Indigenous Scholar’s AwardJ.P.L is supported by an NSERC Discovery Grant(RGPIN-2019-05204)Killam Accelerator Research Fellowship(AWD-018101).
文摘Objectives To determine how the anti-inflammatory actions of interleukin-10(IL-10)and IL-6 differ across age and physical activity levels.Methods Using a cross-sectional design,fasted blood samples were obtained from younger physically inactive(YI:n=10,age:22.7±3.7 years,BMI:24.8±4.8 kg/m^(2),<150 min of weekly moderate-to-vigorous physical activity[MVPA]),younger highly active(YA:n=11 varsity cross country running athletes,20.7±2.7 years,21.1±1.8 kg/m^(2),>300 min of weekly MVPA),and older highly active(OA:12,56.0±10.3 years,22.8±3.2 kg/m^(2),>300 min of weekly MVPA)individuals and analyzed for leukocyte counts,IL-10 and IL-6-related signaling,and cytokine secretion ex vivo.Results Total white blood cells and monocytes were similar between groups(p=0.8)but YA and OA had lower lymphocyte counts than YI(p<0.01).The ability of IL-10(1 ng/mL)to phosphorylate signal transducer and activator of transcription 3(STAT3)in CD14 monocytes was greater in YA vs.YI(p<0.03)despite YA having lower IL-10 receptor expression(p<0.01).IL-6(10 ng/mL)mediated STAT3 phosphorylation in CD4 lymphocytes was higher in OA compared YI(p<0.01),with a similar tendency observed for YA vs.YI(p=0.08).Despite enhanced responsiveness of STAT3 to IL-10/6 in active individuals,the ability of IL-10/6 to inhibit tumor necrosis factor-alpha(TNF-⍺)secretion from lipopolysaccharide-stimulated whole-blood was similar between groups.Conclusions Highly active younger and older individuals demonstrate enhanced IL-10-and IL-6-mediated activation of immune cell STAT3.Although the ability of IL-10/6 to inhibit TNF-⍺secretion appeared unimpacted by activity level,anti-inflammatory cytokine actions were preserved in older active individuals.
