Honey is considered a promising strategy for chronic wound management,being a possible solution for bacterial resistance problem mostly due to its physical-chemical features.However,these properties might vary signifi...Honey is considered a promising strategy for chronic wound management,being a possible solution for bacterial resistance problem mostly due to its physical-chemical features.However,these properties might vary signifi-cantly depending on the honey floral source.Dark-colored honey,such as chestnut honey,is related to increased antioxidant and bactericidal properties.This study aims to assess biofilms of multidrug-resistant microorganisms in honey samples from the Natural Park of Montesinho,in Portugal,which is considered a region with pre-dominant chestnut floral source.The minimum inhibition concentration(MIC)of honey was tested for Staphy-lococcus aureus,Escherichia coli,Klebsiella pneumoniae,Pseudomonas aeruginosa,and Candida albicans.The biofilm removal as well as the metabolic inactivation of the biofilms were evaluated using three honey concentrations:1xMIC,5xMIC,and 10xMIC.The biofilm viability was analyzed with fluorescent microscopy.Honey samples were characterized according to the pollen profile,phenolic composition,H_(2)O_(2) levels,tyrosinase inhibition,and pH.The antioxidant capacity was determined through FRAP and ABTS assays.Most of the honey samples exhibited a MIC of 6.75%.Biofilm removal efficacy differs among samples.Regression analysis evidenced a positive correlation between the biofilm removal and concentrations of H_(2)O_(2),vanillic acid,pinocembrin,Cytisus striatus and Corrigiola telephiifolia,and pH.The honey concentration of 10xMIC was the most effective to inhibit the biofilm metabolism and viability.The concentrations of C.sativa,H_(2)O_(2),and the anti-tyrosinase and FRAP activities were also positively correlated.The honey samples had a dominant presence of Castanea sativa pollen and were effective against biofilms formed by multidrug-resistant microorganisms.展开更多
基金financial support from national funds(FCT/MCTES,FundaçÃo para a Ciancia e Tecnologia and Ministério da Ciencia,Tecnologia e Ensino Superior)through project MTS/SAS/0077/2020-"Honey+-New reasons to care honey from the Natural Park of Montesinho:A bioindicator of environmental quality&its therapeutic potential"and through the project UIDB/50006/2020funded by the Centre for the Research and Technology of Agro-Environmental and Biological Sciences(CITAB)research unit,grant numberUIDB/04033/2020,(https://doi.org/10.54499/UIDB/04033/2020,https://doi.org/10.54499/LA/P/0126/2020(accessedon April 17,2024))+1 种基金The Research Center in Physical Activity,Health and Leisure(CIAFEL),Faculty of Sport,University of Porto(FADEUP)is funded by FCT(UIDB/00617/2020:https://doi.org/10.54499/UIDB/00617/2020)is a member of the Laboratory for Integra-tive and Translational Research in Population Health(ITR),also funded by FCT(LA/P/0064/2020).
文摘Honey is considered a promising strategy for chronic wound management,being a possible solution for bacterial resistance problem mostly due to its physical-chemical features.However,these properties might vary signifi-cantly depending on the honey floral source.Dark-colored honey,such as chestnut honey,is related to increased antioxidant and bactericidal properties.This study aims to assess biofilms of multidrug-resistant microorganisms in honey samples from the Natural Park of Montesinho,in Portugal,which is considered a region with pre-dominant chestnut floral source.The minimum inhibition concentration(MIC)of honey was tested for Staphy-lococcus aureus,Escherichia coli,Klebsiella pneumoniae,Pseudomonas aeruginosa,and Candida albicans.The biofilm removal as well as the metabolic inactivation of the biofilms were evaluated using three honey concentrations:1xMIC,5xMIC,and 10xMIC.The biofilm viability was analyzed with fluorescent microscopy.Honey samples were characterized according to the pollen profile,phenolic composition,H_(2)O_(2) levels,tyrosinase inhibition,and pH.The antioxidant capacity was determined through FRAP and ABTS assays.Most of the honey samples exhibited a MIC of 6.75%.Biofilm removal efficacy differs among samples.Regression analysis evidenced a positive correlation between the biofilm removal and concentrations of H_(2)O_(2),vanillic acid,pinocembrin,Cytisus striatus and Corrigiola telephiifolia,and pH.The honey concentration of 10xMIC was the most effective to inhibit the biofilm metabolism and viability.The concentrations of C.sativa,H_(2)O_(2),and the anti-tyrosinase and FRAP activities were also positively correlated.The honey samples had a dominant presence of Castanea sativa pollen and were effective against biofilms formed by multidrug-resistant microorganisms.
