<b>Context and Aim:</b> Mesenchymal stem cells (MSCs) and platelet-rich fibrin (PRF) have emerged as ideal candidates for advanced therapies of various therapeutically-challenging diseases;however, their r...<b>Context and Aim:</b> Mesenchymal stem cells (MSCs) and platelet-rich fibrin (PRF) have emerged as ideal candidates for advanced therapies of various therapeutically-challenging diseases;however, their regenerative potential in diabetic foot ulcers (DFU) has not been well determined. In this study, we reviewed our clinical experience in mitigating chronic ulcer complications of diabetic foot through a conventional treatment of autologous adipose-derived MSCs embedded in PRF with pure PRF injections. <b>Materials and Methods:</b> The present study was carried out in 10 patients with an open DFU wound selected over a period of 1 year starting from April 2019. Patients were either injected with PRF alone (Group A) or injected with MSCs derived from adipose tissue (ADMSC) embedded in (PRF (Group B). <b>Results:</b> Patients in Group B had a better healing index when compared to Group A. <b>Conclusion:</b> Use of ADMSC embedded in PRF showed promising results to treat DFU.展开更多
Background: Corticosteroid, hyaluronic acid (HA) injections and Anti-inflammatory agents are considered as non-invasive treatments for knee osteoarthritis (OA), Chronic Tendinitis (CT) and Tennis elbow (TE) that are s...Background: Corticosteroid, hyaluronic acid (HA) injections and Anti-inflammatory agents are considered as non-invasive treatments for knee osteoarthritis (OA), Chronic Tendinitis (CT) and Tennis elbow (TE) that are supposed to provide symptomatic relief and to help surgical delay intervention. Platelet rich plasma (PRP) is a biological component shown to be beneficial for different orthopedic dysfunctionalities treatment. The presence of GFs in PRPs such as transforming growth factor-β, insulin-like growth factor 1co-stimulate the mesenchymal stem cells and fibroblasts secretions and promotes the fibrin matrix formation which effectively drive the healing process, induces regenerative response and lead to the damage structure repair in orthopedics trauma. Methods: Three groups of a total of 30 patients presenting OA, CT and TE diagnosis, non-responding to corticosteroid, HA and non-steroid anti-inflammatory treatments were randomized to undergo one intra-articular injections of single high dose of PRP. The efficacy of Intra-articular PRP Injections was evaluated before the injection and one month after. The efficiency assessment score was based on [1] Knee injury and Osteoarthritis Outcome Score, [2] Physical Function Short Form (KOOS-PS) Arabic (KSA) version LK 1.0, [3] HOOS-Physical Function Short form (HOOS-PS), and [4] Macdermid patient-rated Tennis Elbow. Results: A significant reduction of pain and a marked improvement in movements was observed in the 3 patient’s groups, PRP-injected patients showed significantly higher values compared with baseline: (p < 0.005 vs baseline), improve functional status and reduce clearly the articular dysfunctions over the time. In our study, single High dose injection of PRP provided an overall superior clinical improvement compared with HA and corticosteroid treatments over the time and the different follow-up checkpoints of the study.展开更多
Stem cells are the foundation of cellular therapy. They are multipotent cells capable of self-renewing and differentiating into several cell lineages. They are being investigated and used for the treatment of a wide r...Stem cells are the foundation of cellular therapy. They are multipotent cells capable of self-renewing and differentiating into several cell lineages. They are being investigated and used for the treatment of a wide range of diseases. There are various stem cell types and sources, with mesenchymal stem cells standing out as one noteworthy example. With neurological disorders being a major cause of deaths and disabilities worldwide, ongoing studies are investigating the therapeutic potential of mesenchymal stem cells for treating neuropathies. This review comprehensively outlines various neurological diseases and explores the therapeutic potential of mesenchymal stem cells in ischemic stroke, multiple sclerosis, ALS, Alzheimer’s, hypoxia, and glioblastoma. However, there are challenges and limitations in mesenchymal stem cell-based therapies, including concerns about immunocompatibility, maintenance of stemness and differentiation stability, and the potential risk of tumor formation.展开更多
文摘<b>Context and Aim:</b> Mesenchymal stem cells (MSCs) and platelet-rich fibrin (PRF) have emerged as ideal candidates for advanced therapies of various therapeutically-challenging diseases;however, their regenerative potential in diabetic foot ulcers (DFU) has not been well determined. In this study, we reviewed our clinical experience in mitigating chronic ulcer complications of diabetic foot through a conventional treatment of autologous adipose-derived MSCs embedded in PRF with pure PRF injections. <b>Materials and Methods:</b> The present study was carried out in 10 patients with an open DFU wound selected over a period of 1 year starting from April 2019. Patients were either injected with PRF alone (Group A) or injected with MSCs derived from adipose tissue (ADMSC) embedded in (PRF (Group B). <b>Results:</b> Patients in Group B had a better healing index when compared to Group A. <b>Conclusion:</b> Use of ADMSC embedded in PRF showed promising results to treat DFU.
文摘Background: Corticosteroid, hyaluronic acid (HA) injections and Anti-inflammatory agents are considered as non-invasive treatments for knee osteoarthritis (OA), Chronic Tendinitis (CT) and Tennis elbow (TE) that are supposed to provide symptomatic relief and to help surgical delay intervention. Platelet rich plasma (PRP) is a biological component shown to be beneficial for different orthopedic dysfunctionalities treatment. The presence of GFs in PRPs such as transforming growth factor-β, insulin-like growth factor 1co-stimulate the mesenchymal stem cells and fibroblasts secretions and promotes the fibrin matrix formation which effectively drive the healing process, induces regenerative response and lead to the damage structure repair in orthopedics trauma. Methods: Three groups of a total of 30 patients presenting OA, CT and TE diagnosis, non-responding to corticosteroid, HA and non-steroid anti-inflammatory treatments were randomized to undergo one intra-articular injections of single high dose of PRP. The efficacy of Intra-articular PRP Injections was evaluated before the injection and one month after. The efficiency assessment score was based on [1] Knee injury and Osteoarthritis Outcome Score, [2] Physical Function Short Form (KOOS-PS) Arabic (KSA) version LK 1.0, [3] HOOS-Physical Function Short form (HOOS-PS), and [4] Macdermid patient-rated Tennis Elbow. Results: A significant reduction of pain and a marked improvement in movements was observed in the 3 patient’s groups, PRP-injected patients showed significantly higher values compared with baseline: (p < 0.005 vs baseline), improve functional status and reduce clearly the articular dysfunctions over the time. In our study, single High dose injection of PRP provided an overall superior clinical improvement compared with HA and corticosteroid treatments over the time and the different follow-up checkpoints of the study.
文摘Stem cells are the foundation of cellular therapy. They are multipotent cells capable of self-renewing and differentiating into several cell lineages. They are being investigated and used for the treatment of a wide range of diseases. There are various stem cell types and sources, with mesenchymal stem cells standing out as one noteworthy example. With neurological disorders being a major cause of deaths and disabilities worldwide, ongoing studies are investigating the therapeutic potential of mesenchymal stem cells for treating neuropathies. This review comprehensively outlines various neurological diseases and explores the therapeutic potential of mesenchymal stem cells in ischemic stroke, multiple sclerosis, ALS, Alzheimer’s, hypoxia, and glioblastoma. However, there are challenges and limitations in mesenchymal stem cell-based therapies, including concerns about immunocompatibility, maintenance of stemness and differentiation stability, and the potential risk of tumor formation.
