Mulberry,an edible and medicinal fruit rich in polyphenolic compounds that improve gut health and metabolic diseases.Nevertheless,the effects of mulberry polyphenols(MP)against nonalcoholic fatty liver disease(NAFLD)r...Mulberry,an edible and medicinal fruit rich in polyphenolic compounds that improve gut health and metabolic diseases.Nevertheless,the effects of mulberry polyphenols(MP)against nonalcoholic fatty liver disease(NAFLD)remain unknown.This study aimed at utilizing 16s rRNA sequencing,network pharmacology combined transcriptomics technologies to explore the effects and molecular mechanisms of MP on NAFLD.Based on liquid chromatography-electrospray ionization high-resolution mass spectrometry(LC-ESI-HRMS)analysis,40 chemical ingredients were identified in MP.Male C57BL/6J mice were fed a Choline deficient,Lamino acid-defined,high-fat diet(CDAHFD)for six weeks to induce a NAFLD model and treated with MP at low(1 g/kg),medium(2 g/kg)and high(4 g/kg)doses.Pharmacodynamic analysis showed that MP significantly rectified serum biochemical indicators of liver function and lipid metabolism in NAFLD mice.Histopathological evaluation manifested MP was able to attenuate lipid accumulation,reduce hepatocellular necrosis and inflammatory infiltrates.Integrated network pharmacology and liver transcriptome sequencing revealed that MP regulated genes and signaling pathways involved in lipid metabolism and inflammatory response,such as PI3K-Akt,and related core proteins(PI3K,Akt,p-P3K,p-Akt,Nur77,and p21)were further validated by western blotting.Additionally,16s rRNA gene sequencing demonstrated that MP treatment effectively remodeled intestinal microbiota imbalances by reducing possibly harmful bacteria(Romboutsia and Roseburia)and promote potential beneficial bacteria(Muribaculaceae and Bacteroidota).Collectively,this study depicted the comprehensive mechanisms though which MP can alleviate NAFLD,which highlights the nutritional value of MP as a dietary adjuvant for the treatment of NAFLD.展开更多
The fruit of Phyllanthus emblica(PE)is a‘drug homologous food’that is rich in polyphenols for treating in-flammatory diseases.Nevertheless,the ability of PE polyphenols(PEP)to alleviate ulcerative colitis(UC)remains...The fruit of Phyllanthus emblica(PE)is a‘drug homologous food’that is rich in polyphenols for treating in-flammatory diseases.Nevertheless,the ability of PE polyphenols(PEP)to alleviate ulcerative colitis(UC)remains unclear.The aim of this study was to reveal the chemical composition of PEP and its therapeutic potential for treating UC.In this study,PEP was purified with an NKA-2 macroporous resin and identified via liquid chro-matography combined with mass spectrometry(LC‒MS).Furthermore,a dextran sulfate sodium salt-induced UC mice model was used to evaluate the ability of PEP to alleviate UC.The symptoms,histopathology,expression of inflammatory cytokines,mucus and tight junction levels,changes in the gut microbiome and colon tran-scriptomics of UC mice were assessed after PEP intervention.The results suggested that PEP strikingly improved weight loss,disease activity index scores,colon length changes and histopathological damage.Moreover,PEP inhibited the expression of serum proinflammatory cytokines(TNF-α,IL-1β,and IL-6)and sustained intestinal integrity by increasing the expression of Mucin 2,Zonula occludens 1 and Occludin.16S rRNA sequencing revealed that PEP regulated disorders of the gut microflora related to inflammation and the mucus barrier,including Escherichia_Shigella,Rikenellaceae_RC9_gut_group,Bacteroides,Muribaculaceae,Helicobacter and Anae-rostipes.Transcriptome sequencing analysis,western blotting,and immunohistochemistry confirmed that PEP regulated reversed genes as well as effector proteins enriched in the PPAR signaling pathway(PPARγ,MMP1,CYP4A12A,and UCP1)and the Wnt/β-catenin signaling pathway(Wnt8B,FZD9,GSK3β,p-GSK3β,β-catenin,Axin2 and Lgr5).These findings indicate that PEP has therapeutic potential for alleviating UC and could serve as a functional dietary supplement.展开更多
The authors regret the following corrections in the original article:1.Adjust the sequence of Fig.2 C and D and E.The corrected version of Fig.2 provided below:Fig.2.MP attenuates hepatic injury and steatosis in CDAHF...The authors regret the following corrections in the original article:1.Adjust the sequence of Fig.2 C and D and E.The corrected version of Fig.2 provided below:Fig.2.MP attenuates hepatic injury and steatosis in CDAHFD-fed mice.(A)Experimental design(B)Liver index.(C)Serum level of AST.(D)Serum level of ALT.(E)Serum level of TG.展开更多
基金financially supported by the National Natural Science Foundation of China(No.82004069)the Fundamental Research Funds for the Central Universities of Southwest Minzu University(3300224036)the Innovation and Entrepreneurship Training Program of Southwest Minzu University(202310656009).
文摘Mulberry,an edible and medicinal fruit rich in polyphenolic compounds that improve gut health and metabolic diseases.Nevertheless,the effects of mulberry polyphenols(MP)against nonalcoholic fatty liver disease(NAFLD)remain unknown.This study aimed at utilizing 16s rRNA sequencing,network pharmacology combined transcriptomics technologies to explore the effects and molecular mechanisms of MP on NAFLD.Based on liquid chromatography-electrospray ionization high-resolution mass spectrometry(LC-ESI-HRMS)analysis,40 chemical ingredients were identified in MP.Male C57BL/6J mice were fed a Choline deficient,Lamino acid-defined,high-fat diet(CDAHFD)for six weeks to induce a NAFLD model and treated with MP at low(1 g/kg),medium(2 g/kg)and high(4 g/kg)doses.Pharmacodynamic analysis showed that MP significantly rectified serum biochemical indicators of liver function and lipid metabolism in NAFLD mice.Histopathological evaluation manifested MP was able to attenuate lipid accumulation,reduce hepatocellular necrosis and inflammatory infiltrates.Integrated network pharmacology and liver transcriptome sequencing revealed that MP regulated genes and signaling pathways involved in lipid metabolism and inflammatory response,such as PI3K-Akt,and related core proteins(PI3K,Akt,p-P3K,p-Akt,Nur77,and p21)were further validated by western blotting.Additionally,16s rRNA gene sequencing demonstrated that MP treatment effectively remodeled intestinal microbiota imbalances by reducing possibly harmful bacteria(Romboutsia and Roseburia)and promote potential beneficial bacteria(Muribaculaceae and Bacteroidota).Collectively,this study depicted the comprehensive mechanisms though which MP can alleviate NAFLD,which highlights the nutritional value of MP as a dietary adjuvant for the treatment of NAFLD.
基金supported by the National Natural Science Foundation of China(No.82004069)the Fundamental Research Funds for the Central Universities of Southwest Minzu University(ZYN2024035).
文摘The fruit of Phyllanthus emblica(PE)is a‘drug homologous food’that is rich in polyphenols for treating in-flammatory diseases.Nevertheless,the ability of PE polyphenols(PEP)to alleviate ulcerative colitis(UC)remains unclear.The aim of this study was to reveal the chemical composition of PEP and its therapeutic potential for treating UC.In this study,PEP was purified with an NKA-2 macroporous resin and identified via liquid chro-matography combined with mass spectrometry(LC‒MS).Furthermore,a dextran sulfate sodium salt-induced UC mice model was used to evaluate the ability of PEP to alleviate UC.The symptoms,histopathology,expression of inflammatory cytokines,mucus and tight junction levels,changes in the gut microbiome and colon tran-scriptomics of UC mice were assessed after PEP intervention.The results suggested that PEP strikingly improved weight loss,disease activity index scores,colon length changes and histopathological damage.Moreover,PEP inhibited the expression of serum proinflammatory cytokines(TNF-α,IL-1β,and IL-6)and sustained intestinal integrity by increasing the expression of Mucin 2,Zonula occludens 1 and Occludin.16S rRNA sequencing revealed that PEP regulated disorders of the gut microflora related to inflammation and the mucus barrier,including Escherichia_Shigella,Rikenellaceae_RC9_gut_group,Bacteroides,Muribaculaceae,Helicobacter and Anae-rostipes.Transcriptome sequencing analysis,western blotting,and immunohistochemistry confirmed that PEP regulated reversed genes as well as effector proteins enriched in the PPAR signaling pathway(PPARγ,MMP1,CYP4A12A,and UCP1)and the Wnt/β-catenin signaling pathway(Wnt8B,FZD9,GSK3β,p-GSK3β,β-catenin,Axin2 and Lgr5).These findings indicate that PEP has therapeutic potential for alleviating UC and could serve as a functional dietary supplement.
文摘The authors regret the following corrections in the original article:1.Adjust the sequence of Fig.2 C and D and E.The corrected version of Fig.2 provided below:Fig.2.MP attenuates hepatic injury and steatosis in CDAHFD-fed mice.(A)Experimental design(B)Liver index.(C)Serum level of AST.(D)Serum level of ALT.(E)Serum level of TG.
