BACKGROUND Advanced materials and techniques are used to successfully manage the apexification of immature teeth with open apices.The use of mineral trioxide aggregate(MTA),bioceramic sealers,and sodium hypochlorite(N...BACKGROUND Advanced materials and techniques are used to successfully manage the apexification of immature teeth with open apices.The use of mineral trioxide aggregate(MTA),bioceramic sealers,and sodium hypochlorite(NaOCl),combined with internal heating and ultrasonic activation,ensures that canals are cleaned,disinfected,and sealed properly.Magnification devices,such as dental operating microscopes(DOM),provide precise visualization for accurate material placement,while the micro apical placement system ensures void-free MTA delivery.This modern approach improves procedural outcomes,lowers iatrogenic errors,and increases long-term success in apexification,making it a dependable and predictable treatment option for immature teeth.CASE SUMMARY Apexification is a regenerative endodontic procedure that involves creating a calcified barrier at the apex of a nonvital tooth with an open apex.This technique is commonly used in immature teeth with necrotic pulps to ensure proper root canal sealing.Traditionally,calcium hydroxide was the preferred material,but advances have introduced bioceramic cements like MTA or Biodentine,which provide superior results with less treatment time.Apexification not only helps to maintain the tooth's structural integrity but also prevents further complications,making it an important procedure in such cases.CONCLUSION This case demonstrates the effectiveness of integrating advanced materials,precise irrigation protocols,and magnification tools in the apexification of immature teeth with open apices.The use of MTA created a stable apical barrier,while bioceramic sealers enhanced the seal and promoted long-term healing.NaOCl with internal heating,ultrasonic activation,and double-sided vented needles ensured thorough irrigation and disinfection,especially in complex canal anatomy.展开更多
BACKGROUND The primary issue in managing edentulous patients is the severely resorbed mandibular ridge,particularly in older individuals with diminished adaptive capacities.This compromised situation leads to the fabr...BACKGROUND The primary issue in managing edentulous patients is the severely resorbed mandibular ridge,particularly in older individuals with diminished adaptive capacities.This compromised situation leads to the fabrication of inadequate dentures that lack retention and stability,potentially causing psychosocial issues.AIM To determine the difference in retentive capacity between three attachment systems in implant-retained overdentures.METHODS Three edentulous mandibular models were fabricated using heat-cured polymethacrylate resin,with two implant replicas placed in the intra-foraminal region of each model.30 acrylic resin mandibular overdentures were fabricated with provisions for three different overdenture attachment systems:A prefabricated ball/O-ring attachment,a locator attachment system,and an equator attachment system.Each model was subjected to 15000 pulls using a universal testing machine to remove the overdenture from the acrylic model and the force data were recorded.RESULTS The ball/O-ring attachment system demonstrated superior retentive capacity for 15 years,while the locator and equator attachment systems maintained excellent retentive capacity for 5 years.CONCLUSION The ball/O-ring attachment system outperformed better than the other two attachment systems regarding retentive capacity.The locator and equator attachment systems presented sufficient retentive abilities until 15000 cycles.After 7500 cycles,significant differences in retentive force between the systems evolved.展开更多
During restorative dental procedures,complete control over the operative site is critical for patient comfort,safety,and the operator’s access and visibility.The success of a fixed prosthesis depends on accurate impr...During restorative dental procedures,complete control over the operative site is critical for patient comfort,safety,and the operator’s access and visibility.The success of a fixed prosthesis depends on accurate impression making of the prepared finish lines on the abutment teeth.To optimise long-term outcomes for the fixed restoration,gingival retraction techniques should be used to decrease the marginal discrepancy among the restoration and the prepared abutment.Accurate marginal positioning of the restoration along the prepared finish line of the abutment is essential for therapeutic,preventive,and aesthetic purposes.展开更多
A simple, precise and rapid liquid chromatography-tandem mass spectrometry （LC-MS/MS） method has been developed and validated for the simultaneous determination of oseltamivir and oseltamivir carboxylate, a neuramin...A simple, precise and rapid liquid chromatography-tandem mass spectrometry （LC-MS/MS） method has been developed and validated for the simultaneous determination of oseltamivir and oseltamivir carboxylate, a neuraminidase inhibitor, using their deuterated analogs as internal standards （ISs）. The method involved solid phase extraction of the analytes and ISs from 200 μL human plasma with no reconstitution and drying steps. The chromatographic separation was achieved on a Symmetry C18 （100 mm × 4.6 mm, 5 μm） column using 10 mM ammonium formate and acetonitrile （30：70, v/v） as the mobile phase in a run time of 2.0 min. Quantitation of analytes and ISs were done by multiple reaction monitoring on a triple quadrupole mass spectrometer in the positive ionization mode. The linearity of the method was established in the concentration range of 0.5-200 ng/mL and 2.0-800 ng/mL for oseltamivir and oseltamivir carboxylate respectively. The mean extraction recovery for oseltamivir （94.4%） and oseltamivir carboxylate （92.7%） from spiked plasma samples was consistent and reproducible. The application of this method was demonstratedby a bioequivalence study in 42 healthy Indian subjects with 75 mg oseltamivir phosphate capsules. The assay reproducibility was established by reanalysis of 151 incurred subject samples.展开更多
Background One potential modifiable factor to improve the mental health of healthcare professionals(HCPs)during the pandemic is lifestyle.Aims This study aimed to assess whether an improved lifestyle during the pandem...Background One potential modifiable factor to improve the mental health of healthcare professionals(HCPs)during the pandemic is lifestyle.Aims This study aimed to assess whether an improved lifestyle during the pandemic is associated with improved mental health symptoms and mental well-being in HCPs over time.Methods This was a cohort study involving an online survey distributed at two separate time points during the pandemic(baseline(July–September 2020)and follow-up(December 2020–March 2021))to HCPs working in primary or secondary care in the UK.Both surveys assessed for major depressive disorder(MDD)(Patient Health Questionnaire-9(PHQ-9)),generalised anxiety disorder(GAD)(Generalised Anxiety Disorder-7(GAD-7)),mental well-being(Short Warwick-Edinburgh Mental Well-being Score(SWEMWBS))and self-reported lifestyle change(compared with the start of the pandemic)on multiple domains.Cumulative scores were calculated to estimate overall lifestyle change compared with that before the pandemic(at both baseline and follow-up).At each time point,separate logistic regression models were constructed to relate the lifestyle change score with the presence of MDD,GAD and low mental well-being.Linear regression models were also developed relating the change in lifestyle scores from baseline to follow-up to changes in PHQ-9,GAD-7 and SWEMWBS scores.Results 613 HCPs completed both baseline assessment and follow-up assessment.Consistent significant cross-sectional associations between increased lifestyle change scores and a reduced risk of MDD,GAD and low mental well-being were observed at both baseline and follow-up.Over the study period,a whole unit increase in the change in novel scores(ie,improved overall lifestyle)over 4 months was inversely associated with changes in PHQ-9(adjusted coefficient:−0.51,95%confidence interval(CI):−0.73 to−0.30,p<0.001)and GAD-7 scores(adjusted coefficient:−0.32,95%CI:−0.53 to−0.10,p=0.004)and positively associated with the change in SWEMWBS scores(adjusted coefficient:0.37,95%CI:0.18 to 0.55,p<0.001).Conclusions Improved lifestyle over time is associated with improved mental health and mental well-being in HCPs during the pandemic.Improving lifestyle could be a recommended intervention for HCPs to help mitigate the mental health impact during the current and future pandemics.展开更多
A selective and rapid high-performance liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of raltegravir using raltegravir-d3 as an internal standard（IS）. The a...A selective and rapid high-performance liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of raltegravir using raltegravir-d3 as an internal standard（IS）. The analyte and IS were extracted with methylene chloride and n-hexane solvent mixture from 100 mL human plasma. The chromatographic separation was achieved on a Chromolith RP-18 e endcapped C18（100 mm 4.6 mm） column in a run time of 2.0 min. Quantitation was performed in the negative ionization mode using the transitions of m/z 443.1-316.1 for raltegravir and m/z 446.1-319.0 for IS. The linearity of the method was established in the concentration range of 2.0–6000 ng/m L.The mean extraction recovery for raltegravir and IS was 92.6% and 91.8%, respectively, and the IS-normalized matrix factors for raltegravir ranged from 0.992 to 0.999. The application of this method was demonstrated by a bioequivalence study on 18 healthy subjects.展开更多
Prime editing(PE)technology enables precise alterations in the genetic code of a genome of interest.PE offers great potential for identifying major agronomically important genes in plants and editing them into superio...Prime editing(PE)technology enables precise alterations in the genetic code of a genome of interest.PE offers great potential for identifying major agronomically important genes in plants and editing them into superior variants,ideally targeting multiple loci simultaneously to realize the collective effects of the edits.Here,we report the development of a modular assembly-based multiplex PE system in rice and demon-strate its efficacy in editing up to four genes in a single transformation experiment.The duplex PE(DPE)system achieved a co-editing efficiency of 46.1%in the T0 generation,converting TFIIAg5 to xa5 and xa23 to Xa23SW11.The resulting double-mutant lines exhibited robust broad-spectrum resistance against multiple Xanthomonas oryzae pathovar oryzae(Xoo)strains in the T1 generation.In addition,we success-fully edited OsEPSPS1 to an herbicide-tolerant variant and OsSWEET11a to a Xoo-resistant allele,achieving a co-editing rate of 57.14%.Furthermore,with the quadruple PE(QPE)system,we edited four genes—two for herbicide tolerance(OsEPSPS1 and OsALS1)and two for Xoo resistance(TFIIAg5 and OsSWEET11a)—using one construct,with a co-editing efficiency of 43.5%for all four genes in the T0 gen-eration.We performed multiplex PE usingfive more constructs,including two for triplex PE(TPE)and three for QPE,each targeting a different set of genes.The editing rates were dependent on the activity of pegRNA and/or ngRNA.For instance,optimization of ngRNA increased the PE rates for one of the targets(OsSPL13)from 0%to 30%but did not improve editing at another target(OsGS2).Overall,our modular assembly-based system yielded high PE rates and streamlined the cloning of PE reagents,making it feasible for more labs to utilize PE for their editing experiments.Thesefindings have significant implications for advancing gene editing techniques in plants and may pave the way for future agricultural applications.展开更多
Evasion of immune surveillance is one of the hallmarks of cancer.Although the adaptive immune system has been targeted via checkpoint inhibition,many patients do not sustain durable remissions due to the heterogeneity...Evasion of immune surveillance is one of the hallmarks of cancer.Although the adaptive immune system has been targeted via checkpoint inhibition,many patients do not sustain durable remissions due to the heterogeneity of the tumor microenvironment,so additional strategies are needed.The innate immune system has its own set of checkpoints,and tumors have co-opted this system by expressing surface receptors that inhibit phagocytosis.One of these receptors,CD47,also known as the“don’t eat me”signal,has been found to be overexpressed by most cancer histologies and has been successfully targeted by antibodies blocking the receptor or its ligand,signal regulatory proteinα(SIRPα).By enabling phagocytosis via antigen-presenting cells,interruption of CD47-SIRPαbinding leads to earlier downstream activation of the adaptive immune system.Recent and ongoing clinical trials are demonstrating the safety and efficacy of CD47 blockade in combination with monoclonal antibodies,chemotherapy,or checkpoint inhibitors for adult cancer histologies.The aim of this review is to highlight the current literature and research on CD47,provide an impetus for investigation of its blockade in pediatric cancer histologies,and provide a rationale for new combination therapies in these patients.展开更多
Background and Purpose:With the increasing prevalence of cerebrovascular risk factors in young adults,accurate differentiation of multiple sclerosis(MS)from cerebral small vessel disease(SVD)remains challenging becaus...Background and Purpose:With the increasing prevalence of cerebrovascular risk factors in young adults,accurate differentiation of multiple sclerosis(MS)from cerebral small vessel disease(SVD)remains challenging because of the significant overlap in the appearance of white matter lesions on conventional magnetic resonance(MR)images.The purpose of this study was to use quantitative susceptibility mapping(QSM)to differentiate patients with MS from patients with SVD.Materials and Methods:Thirty-two patients with relapsing-remitting MS and 40 patients with SVD were selected in this institution review board-approved retrospective study.All patient examinations included QSM and T2-weighted FLAIR(T2w).All T2w hyperintense lesions were analyzed on QSM for susceptibility relative to normal-appearing white matter and were evaluated for the presence of hyperintense rims and the central vein sign.Receiver operating characteristic(ROC)curve analysis was performed to assess the diagnostic accuracy of differentiating MS patients from SVD patients.Results:The mean susceptibility value of SVD lesions was significantly lower than MS lesions(2.31±9.68 ppb vs.27.77±20.27 ppb,p<0.001).Of MS patients and MS lesions,68.8%and 22.2%had hyperintense rims on QSM,respectively.Of MS patients and MS lesions,75%and 37.6%had a central vein sign on QSM,respectively.Lesion susceptibility values allowed accurate differentiation MS from SVD with an area under the ROC curve(AUC)=0.848 for lesion differentiation,AUC=0.999 for patient differentiation,and an optimal cut-off susceptibility value of 14.88 ppb.Conclusions:Magnetic susceptibility measured using QSM allows accurate differentiation of MS patients from SVD patients.展开更多
文摘BACKGROUND Advanced materials and techniques are used to successfully manage the apexification of immature teeth with open apices.The use of mineral trioxide aggregate(MTA),bioceramic sealers,and sodium hypochlorite(NaOCl),combined with internal heating and ultrasonic activation,ensures that canals are cleaned,disinfected,and sealed properly.Magnification devices,such as dental operating microscopes(DOM),provide precise visualization for accurate material placement,while the micro apical placement system ensures void-free MTA delivery.This modern approach improves procedural outcomes,lowers iatrogenic errors,and increases long-term success in apexification,making it a dependable and predictable treatment option for immature teeth.CASE SUMMARY Apexification is a regenerative endodontic procedure that involves creating a calcified barrier at the apex of a nonvital tooth with an open apex.This technique is commonly used in immature teeth with necrotic pulps to ensure proper root canal sealing.Traditionally,calcium hydroxide was the preferred material,but advances have introduced bioceramic cements like MTA or Biodentine,which provide superior results with less treatment time.Apexification not only helps to maintain the tooth's structural integrity but also prevents further complications,making it an important procedure in such cases.CONCLUSION This case demonstrates the effectiveness of integrating advanced materials,precise irrigation protocols,and magnification tools in the apexification of immature teeth with open apices.The use of MTA created a stable apical barrier,while bioceramic sealers enhanced the seal and promoted long-term healing.NaOCl with internal heating,ultrasonic activation,and double-sided vented needles ensured thorough irrigation and disinfection,especially in complex canal anatomy.
文摘BACKGROUND The primary issue in managing edentulous patients is the severely resorbed mandibular ridge,particularly in older individuals with diminished adaptive capacities.This compromised situation leads to the fabrication of inadequate dentures that lack retention and stability,potentially causing psychosocial issues.AIM To determine the difference in retentive capacity between three attachment systems in implant-retained overdentures.METHODS Three edentulous mandibular models were fabricated using heat-cured polymethacrylate resin,with two implant replicas placed in the intra-foraminal region of each model.30 acrylic resin mandibular overdentures were fabricated with provisions for three different overdenture attachment systems:A prefabricated ball/O-ring attachment,a locator attachment system,and an equator attachment system.Each model was subjected to 15000 pulls using a universal testing machine to remove the overdenture from the acrylic model and the force data were recorded.RESULTS The ball/O-ring attachment system demonstrated superior retentive capacity for 15 years,while the locator and equator attachment systems maintained excellent retentive capacity for 5 years.CONCLUSION The ball/O-ring attachment system outperformed better than the other two attachment systems regarding retentive capacity.The locator and equator attachment systems presented sufficient retentive abilities until 15000 cycles.After 7500 cycles,significant differences in retentive force between the systems evolved.
文摘During restorative dental procedures,complete control over the operative site is critical for patient comfort,safety,and the operator’s access and visibility.The success of a fixed prosthesis depends on accurate impression making of the prepared finish lines on the abutment teeth.To optimise long-term outcomes for the fixed restoration,gingival retraction techniques should be used to decrease the marginal discrepancy among the restoration and the prepared abutment.Accurate marginal positioning of the restoration along the prepared finish line of the abutment is essential for therapeutic,preventive,and aesthetic purposes.
文摘A simple, precise and rapid liquid chromatography-tandem mass spectrometry （LC-MS/MS） method has been developed and validated for the simultaneous determination of oseltamivir and oseltamivir carboxylate, a neuraminidase inhibitor, using their deuterated analogs as internal standards （ISs）. The method involved solid phase extraction of the analytes and ISs from 200 μL human plasma with no reconstitution and drying steps. The chromatographic separation was achieved on a Symmetry C18 （100 mm × 4.6 mm, 5 μm） column using 10 mM ammonium formate and acetonitrile （30：70, v/v） as the mobile phase in a run time of 2.0 min. Quantitation of analytes and ISs were done by multiple reaction monitoring on a triple quadrupole mass spectrometer in the positive ionization mode. The linearity of the method was established in the concentration range of 0.5-200 ng/mL and 2.0-800 ng/mL for oseltamivir and oseltamivir carboxylate respectively. The mean extraction recovery for oseltamivir （94.4%） and oseltamivir carboxylate （92.7%） from spiked plasma samples was consistent and reproducible. The application of this method was demonstratedby a bioequivalence study in 42 healthy Indian subjects with 75 mg oseltamivir phosphate capsules. The assay reproducibility was established by reanalysis of 151 incurred subject samples.
基金This work was supported by a Barts Charity project grant(MGU0586).
文摘Background One potential modifiable factor to improve the mental health of healthcare professionals(HCPs)during the pandemic is lifestyle.Aims This study aimed to assess whether an improved lifestyle during the pandemic is associated with improved mental health symptoms and mental well-being in HCPs over time.Methods This was a cohort study involving an online survey distributed at two separate time points during the pandemic(baseline(July–September 2020)and follow-up(December 2020–March 2021))to HCPs working in primary or secondary care in the UK.Both surveys assessed for major depressive disorder(MDD)(Patient Health Questionnaire-9(PHQ-9)),generalised anxiety disorder(GAD)(Generalised Anxiety Disorder-7(GAD-7)),mental well-being(Short Warwick-Edinburgh Mental Well-being Score(SWEMWBS))and self-reported lifestyle change(compared with the start of the pandemic)on multiple domains.Cumulative scores were calculated to estimate overall lifestyle change compared with that before the pandemic(at both baseline and follow-up).At each time point,separate logistic regression models were constructed to relate the lifestyle change score with the presence of MDD,GAD and low mental well-being.Linear regression models were also developed relating the change in lifestyle scores from baseline to follow-up to changes in PHQ-9,GAD-7 and SWEMWBS scores.Results 613 HCPs completed both baseline assessment and follow-up assessment.Consistent significant cross-sectional associations between increased lifestyle change scores and a reduced risk of MDD,GAD and low mental well-being were observed at both baseline and follow-up.Over the study period,a whole unit increase in the change in novel scores(ie,improved overall lifestyle)over 4 months was inversely associated with changes in PHQ-9(adjusted coefficient:−0.51,95%confidence interval(CI):−0.73 to−0.30,p<0.001)and GAD-7 scores(adjusted coefficient:−0.32,95%CI:−0.53 to−0.10,p=0.004)and positively associated with the change in SWEMWBS scores(adjusted coefficient:0.37,95%CI:0.18 to 0.55,p<0.001).Conclusions Improved lifestyle over time is associated with improved mental health and mental well-being in HCPs during the pandemic.Improving lifestyle could be a recommended intervention for HCPs to help mitigate the mental health impact during the current and future pandemics.
文摘A selective and rapid high-performance liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of raltegravir using raltegravir-d3 as an internal standard（IS）. The analyte and IS were extracted with methylene chloride and n-hexane solvent mixture from 100 mL human plasma. The chromatographic separation was achieved on a Chromolith RP-18 e endcapped C18（100 mm 4.6 mm） column in a run time of 2.0 min. Quantitation was performed in the negative ionization mode using the transitions of m/z 443.1-316.1 for raltegravir and m/z 446.1-319.0 for IS. The linearity of the method was established in the concentration range of 2.0–6000 ng/m L.The mean extraction recovery for raltegravir and IS was 92.6% and 91.8%, respectively, and the IS-normalized matrix factors for raltegravir ranged from 0.992 to 0.999. The application of this method was demonstrated by a bioequivalence study on 18 healthy subjects.
基金supported by an NSF award (IOS-2210259 to B.Y.)a subaward to the University of Missouri from the Heinrich Heine University of Dusseldorf funded by the Bill&Melinda Gates Foundation (OPP1155704)supported by the Daniel Millikan Award for Outstanding Research in Plant–Microbe Interactions at the University of Missouri.
文摘Prime editing(PE)technology enables precise alterations in the genetic code of a genome of interest.PE offers great potential for identifying major agronomically important genes in plants and editing them into superior variants,ideally targeting multiple loci simultaneously to realize the collective effects of the edits.Here,we report the development of a modular assembly-based multiplex PE system in rice and demon-strate its efficacy in editing up to four genes in a single transformation experiment.The duplex PE(DPE)system achieved a co-editing efficiency of 46.1%in the T0 generation,converting TFIIAg5 to xa5 and xa23 to Xa23SW11.The resulting double-mutant lines exhibited robust broad-spectrum resistance against multiple Xanthomonas oryzae pathovar oryzae(Xoo)strains in the T1 generation.In addition,we success-fully edited OsEPSPS1 to an herbicide-tolerant variant and OsSWEET11a to a Xoo-resistant allele,achieving a co-editing rate of 57.14%.Furthermore,with the quadruple PE(QPE)system,we edited four genes—two for herbicide tolerance(OsEPSPS1 and OsALS1)and two for Xoo resistance(TFIIAg5 and OsSWEET11a)—using one construct,with a co-editing efficiency of 43.5%for all four genes in the T0 gen-eration.We performed multiplex PE usingfive more constructs,including two for triplex PE(TPE)and three for QPE,each targeting a different set of genes.The editing rates were dependent on the activity of pegRNA and/or ngRNA.For instance,optimization of ngRNA increased the PE rates for one of the targets(OsSPL13)from 0%to 30%but did not improve editing at another target(OsGS2).Overall,our modular assembly-based system yielded high PE rates and streamlined the cloning of PE reagents,making it feasible for more labs to utilize PE for their editing experiments.Thesefindings have significant implications for advancing gene editing techniques in plants and may pave the way for future agricultural applications.
文摘Evasion of immune surveillance is one of the hallmarks of cancer.Although the adaptive immune system has been targeted via checkpoint inhibition,many patients do not sustain durable remissions due to the heterogeneity of the tumor microenvironment,so additional strategies are needed.The innate immune system has its own set of checkpoints,and tumors have co-opted this system by expressing surface receptors that inhibit phagocytosis.One of these receptors,CD47,also known as the“don’t eat me”signal,has been found to be overexpressed by most cancer histologies and has been successfully targeted by antibodies blocking the receptor or its ligand,signal regulatory proteinα(SIRPα).By enabling phagocytosis via antigen-presenting cells,interruption of CD47-SIRPαbinding leads to earlier downstream activation of the adaptive immune system.Recent and ongoing clinical trials are demonstrating the safety and efficacy of CD47 blockade in combination with monoclonal antibodies,chemotherapy,or checkpoint inhibitors for adult cancer histologies.The aim of this review is to highlight the current literature and research on CD47,provide an impetus for investigation of its blockade in pediatric cancer histologies,and provide a rationale for new combination therapies in these patients.
文摘Background and Purpose:With the increasing prevalence of cerebrovascular risk factors in young adults,accurate differentiation of multiple sclerosis(MS)from cerebral small vessel disease(SVD)remains challenging because of the significant overlap in the appearance of white matter lesions on conventional magnetic resonance(MR)images.The purpose of this study was to use quantitative susceptibility mapping(QSM)to differentiate patients with MS from patients with SVD.Materials and Methods:Thirty-two patients with relapsing-remitting MS and 40 patients with SVD were selected in this institution review board-approved retrospective study.All patient examinations included QSM and T2-weighted FLAIR(T2w).All T2w hyperintense lesions were analyzed on QSM for susceptibility relative to normal-appearing white matter and were evaluated for the presence of hyperintense rims and the central vein sign.Receiver operating characteristic(ROC)curve analysis was performed to assess the diagnostic accuracy of differentiating MS patients from SVD patients.Results:The mean susceptibility value of SVD lesions was significantly lower than MS lesions(2.31±9.68 ppb vs.27.77±20.27 ppb,p<0.001).Of MS patients and MS lesions,68.8%and 22.2%had hyperintense rims on QSM,respectively.Of MS patients and MS lesions,75%and 37.6%had a central vein sign on QSM,respectively.Lesion susceptibility values allowed accurate differentiation MS from SVD with an area under the ROC curve(AUC)=0.848 for lesion differentiation,AUC=0.999 for patient differentiation,and an optimal cut-off susceptibility value of 14.88 ppb.Conclusions:Magnetic susceptibility measured using QSM allows accurate differentiation of MS patients from SVD patients.
